##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065365_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70095226
Total Bases	10.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16203579969911	32.0	32.0	32.0	32.0	32.0
2	31.422336123147673	32.0	32.0	32.0	32.0	32.0
3	34.967019965097194	37.0	32.0	37.0	32.0	37.0
4	35.82042851249242	37.0	37.0	37.0	32.0	37.0
5	36.131267670069285	37.0	37.0	37.0	37.0	37.0
6	39.67505611580452	41.0	41.0	41.0	37.0	41.0
7	39.837333886333425	41.0	41.0	41.0	37.0	41.0
8	39.943932372798116	41.0	41.0	41.0	37.0	41.0
9	39.97914889382053	41.0	41.0	41.0	37.0	41.0
10-14	39.99927274647777	41.0	41.0	41.0	37.0	41.0
15-19	39.98765854610413	41.0	41.0	41.0	37.0	41.0
20-24	39.95218379636867	41.0	41.0	41.0	37.0	41.0
25-29	39.825549129408614	41.0	41.0	41.0	37.0	41.0
30-34	39.73302353572552	41.0	41.0	41.0	37.0	41.0
35-39	39.6846199397374	41.0	41.0	41.0	37.0	41.0
40-44	39.610586912152904	41.0	41.0	41.0	37.0	41.0
45-49	39.563899986569695	41.0	41.0	41.0	37.0	41.0
50-54	39.48811459142738	41.0	41.0	41.0	37.0	41.0
55-59	39.41188314022984	41.0	41.0	41.0	37.0	41.0
60-64	39.3162267313326	41.0	41.0	41.0	37.0	41.0
65-69	39.20451876137756	41.0	41.0	41.0	37.0	41.0
70-74	39.123066386860636	41.0	41.0	41.0	37.0	41.0
75-79	38.94829470126824	41.0	40.2	41.0	36.0	41.0
80-84	39.1821936917644	41.0	41.0	41.0	37.0	41.0
85-89	39.093254436471895	41.0	41.0	41.0	37.0	41.0
90-94	38.98918369134012	41.0	41.0	41.0	37.0	41.0
95-99	38.86263691624306	41.0	41.0	41.0	37.0	41.0
100-104	38.73173304270394	41.0	41.0	41.0	32.0	41.0
105-109	38.60333260927071	41.0	41.0	41.0	32.0	41.0
110-114	38.456674025132614	41.0	41.0	41.0	32.0	41.0
115-119	38.30250721211741	41.0	37.8	41.0	32.0	41.0
120-124	38.06531430257461	41.0	37.0	41.0	32.0	41.0
125-129	37.883346666148135	41.0	37.0	41.0	32.0	41.0
130-134	37.65252296640002	41.0	37.0	41.0	29.0	41.0
135-139	37.37198072233907	41.0	37.0	41.0	27.0	41.0
140-144	37.1259010649313	41.0	37.0	41.0	27.0	41.0
145-149	36.861352860179096	41.0	37.0	41.0	27.0	41.0
150	36.695140564922355	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	7.0
13	7.0
14	19.0
15	84.0
16	300.0
17	1058.0
18	2468.0
19	4254.0
20	7517.0
21	12821.0
22	21760.0
23	36505.0
24	60693.0
25	99170.0
26	154225.0
27	232991.0
28	334901.0
29	468855.0
30	637477.0
31	840985.0
32	1091722.0
33	1396205.0
34	1782836.0
35	2286086.0
36	3010360.0
37	4186917.0
38	6567758.0
39	1.354773E7
40	3.3309515E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.832854865272907	39.950457989187576	44.83472055362257	0.381966591916948
2	16.963302118024913	40.08307561679559	42.69495986087573	0.2586624043037699
3	16.06873377042152	36.81046622874532	46.82165296350033	0.299147037332833
4	16.417397519040247	36.09637187008332	47.171354557136546	0.314876053739889
5	16.504981380614993	36.09443387770802	47.08810697036628	0.3124777713107024
6	16.56564710953777	35.556359922244575	47.55057669948257	0.3274162687350811
7	17.132168090710223	35.770055288998414	46.79407339372188	0.3037032265694778
8	16.377450977175243	35.2328816895124	48.076310668093335	0.31335666521901656
9	16.76164473974981	35.232746425193675	47.692845291411565	0.3127635436449604
10-14	16.620307189100004	35.812794786676825	47.27042231948006	0.2964757047431088
15-19	16.612345966030194	35.411592000794236	47.67804338251815	0.29801865065742417
20-24	16.775324133610987	35.449799039914595	47.47250068314417	0.3023761433302517
25-29	16.892580070016404	35.20257064567074	47.592974421599536	0.3118748627133264
30-34	16.982629854520255	35.21856087269017	47.48455395807232	0.31425531471725937
35-39	17.003098996926887	35.10734235155589	47.57421859304682	0.3153400584703933
40-44	17.002380182104453	35.16134536692511	47.51754241278663	0.3187320381838056
45-49	16.992821581737807	35.12385944722469	47.56587226333922	0.31744670769827854
50-54	16.988272592601266	35.15146468995513	47.54071158135682	0.3195511360867871
55-59	16.98258818506046	35.15151021103569	47.54479350722763	0.32110809667621376
60-64	16.959656183171944	35.17681671407954	47.539350037470115	0.3241770652784005
65-69	16.963609211232434	35.16246035959145	47.548107470185805	0.3258229589903061
70-74	16.952594207310046	35.174401440205855	47.54324383609229	0.32976051639181053
75-79	16.945732005741515	35.17686544486201	47.554472334204284	0.3229302151921956
80-84	16.937570817219772	35.17135492738765	47.56740634315196	0.3236679122406307
85-89	16.94557751098154	35.18009002768008	47.53541514746633	0.3389173138720471
90-94	16.925356218137246	35.17004679629906	47.568736487713416	0.335860497850279
95-99	16.92842512204594	35.1846410265137	47.5449693119238	0.3419645395165605
100-104	16.897448396845498	35.180050843422705	47.57907037224495	0.34343038748684235
105-109	16.898953052516106	35.201355206610465	47.54780614077453	0.351885600098892
110-114	16.891757340870747	35.18941333169563	47.569836582994405	0.348992744439211
115-119	16.865115633406255	35.19685440311729	47.58091375594512	0.35711620753133316
120-124	16.860677057854524	35.18557535403619	47.583049865216935	0.3706977228923479
125-129	16.861392892380223	35.20084712488614	47.5599756351958	0.37778434753784884
130-134	16.846447569168074	35.19489048448203	47.56677159662308	0.3918903497268162
135-139	16.846398457794127	35.182055672201464	47.56327864997033	0.40826722003408566
140-144	16.831895902660975	35.17281289038874	47.57868014981797	0.4166110571323142
145-149	16.825128373817606	35.16132745708484	47.584058173827245	0.4294859952703177
150	16.810330535852483	35.18005181709373	47.57689115585424	0.4327264911995459
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4923.0
1	6877.0
2	13138.0
3	27674.5
4	60749.0
5	131603.0
6	267933.5
7	506008.5
8	883718.0
9	1419057.0
10	2103461.0
11	2892168.0
12	3700076.0
13	4434355.5
14	5013923.0
15	5380497.5
16	5507585.5
17	5397810.0
18	5087140.0
19	4621177.0
20	4065482.5
21	3482948.0
22	2914411.0
23	2395799.0
24	1946797.5
25	1572826.5
26	1263561.0
27	1007131.5
28	799249.5
29	637582.5
30	512262.0
31	410261.0
32	325098.5
33	254744.0
34	199138.0
35	157110.0
36	125341.0
37	100380.0
38	80037.5
39	64238.5
40	52116.5
41	42034.5
42	33878.0
43	27553.5
44	22273.0
45	18080.0
46	14698.0
47	12131.5
48	10197.5
49	10499.0
50	11811.0
51	11633.5
52	9892.0
53	7529.5
54	6034.5
55	5054.5
56	4174.5
57	3569.5
58	3224.5
59	2839.5
60	2373.0
61	1995.0
62	1568.5
63	1170.0
64	970.0
65	762.0
66	589.0
67	477.5
68	393.5
69	308.5
70	236.5
71	200.5
72	174.0
73	143.5
74	117.5
75	96.5
76	90.0
77	89.0
78	94.5
79	96.5
80	77.0
81	66.0
82	57.0
83	46.5
84	48.0
85	44.5
86	32.0
87	23.5
88	18.0
89	16.5
90	14.0
91	11.0
92	7.5
93	5.0
94	7.5
95	10.0
96	12.0
97	11.5
98	22.5
99	77.0
100	847.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.753844491492188
2	0.001101358885696438
3	8.545517778914073E-4
4	0.008645381926580847
5	0.0
6	7.561142609055858E-5
7	0.0011569974822536416
8	2.8532613619078706E-5
9	1.4266306809539354E-4
10-14	2.4794841234979396E-4
15-19	3.09578857767004E-4
20-24	3.024457043622343E-4
25-29	0.001459157860479685
30-34	3.5095114751466813E-4
35-39	9.62119731235334E-4
40-44	6.008968428177976E-4
45-49	0.0011627040049774573
50-54	6.656658757331062E-4
55-59	2.870380930079318E-4
60-64	9.886550619010773E-4
65-69	0.0011119159527354973
70-74	5.101631315091274E-4
75-79	3.817663702232731E-4
80-84	0.001743057365989518
85-89	0.0011184784538678853
90-94	5.840626007825412E-4
95-99	0.001842636187520103
100-104	0.002951984205029883
105-109	2.4595112939645845E-4
110-114	3.486685384251418E-4
115-119	5.643750973853769E-4
120-124	3.104348361755764E-4
125-129	0.0014320518775415604
130-134	0.0010288860471039782
135-139	0.0016180845183379536
140-144	0.0011855300958727203
145-149	0.0018531932545591619
150	0.0058063868714825165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.0095226E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.72286734821685
#Duplication Level	Percentage of total
1	74.60559698311971
2	16.100663285915996
3	3.9078805931760607
4	1.2863245382404427
5	0.6982800971610112
6	0.49029234155832707
7	0.3391659313462697
8	0.2912344164698806
9	0.2241293049502654
>10	1.7449254066976245
>50	0.09583916880603584
>100	0.13448232254294576
>500	0.030593959792582014
>1k	0.0320864141383512
>5k	0.018505236084451804
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.4266306809539355E-5	0.0	0.0	0.0	0.003446739725184708	0.001813247595492452
2	1.4266306809539355E-5	0.0	0.0	0.0	0.01117622475459313	0.005658017280663308
3	1.7119568171447224E-5	0.0	0.0	0.0	0.017354962233804623	0.00850414548916641
4	1.7119568171447224E-5	0.0	0.0	0.0	0.023694908979963913	0.0116726922315651
5	1.7119568171447224E-5	0.0	0.0	0.0	0.03025455685099011	0.014972488996611553
6	1.9972829533355097E-5	0.0	0.0	0.0	0.036691514483454264	0.018885736954468196
7	2.139946021430903E-5	0.0	0.0	0.0	0.04339525205325681	0.02304151212808701
8	2.2826090895262967E-5	0.0	0.0	0.0	0.049495524845015834	0.026826363324657803
9	2.7105982938124773E-5	0.0	0.0	0.0	0.055635743295841575	0.030930779793762273
10-11	2.853261361907871E-5	0.0	0.0	0.0	0.06472837394090147	0.03717086239225479
12-13	3.0672559640509614E-5	0.0	0.0	0.0	0.07588105928925887	0.045981020162485814
14-15	3.637908236432535E-5	0.0	0.0	0.0	0.08636180158688696	0.05525982611141021
16-17	4.4225551109572E-5	0.0	0.0	0.0	0.09674909957491257	0.064898142991935
18-19	5.777854257863438E-5	0.0	0.0	0.0	0.10674764070237822	0.07462847755138131
20-21	7.846468745246644E-5	0.0	0.0	7.133153404769677E-7	0.11644016954877925	0.08432671292050617
22-23	1.0343072436916032E-4	0.0	0.0	1.4266306809539354E-6	0.12564407738695357	0.09350708135244475
24-25	1.32676653328716E-4	0.0	0.0	1.4266306809539354E-6	0.13467108302068959	0.10281156665362631
26-27	1.7048236637399528E-4	0.0	0.0	1.4266306809539354E-6	0.14362747043571839	0.1120411538440578
28-29	2.032948720359358E-4	0.0	0.0	1.4266306809539354E-6	0.15270084156658542	0.12123364863678449
30-31	2.3468074701692236E-4	0.0	0.0	1.4266306809539354E-6	0.16195396816325267	0.13048463528743026
32-33	2.9745249697889556E-4	0.0	0.0	1.4266306809539354E-6	0.17108725778271974	0.13985117902323335
34-35	3.545177242170529E-4	0.0	0.0	1.4266306809539354E-6	0.18047448766339663	0.1490015882108719
36-37	3.9660332930519405E-4	0.0	0.0	1.4266306809539354E-6	0.18989310341905452	0.15816840365134138
38-39	4.4582208779810485E-4	0.0	0.0	1.4266306809539354E-6	0.19916120963787176	0.16695858859203905
40-41	5.028873150362623E-4	0.0	0.0	2.139946021430903E-6	0.2087545876519465	0.17583508468893444
42-43	5.628058036363275E-4	0.0	0.0	2.853261361907871E-6	0.21810401181957811	0.18476436612102515
44-45	6.077446700863765E-4	0.0	0.0	4.279892042861806E-6	0.22759253247860275	0.1935395714395728
46-47	6.747963120912115E-4	0.0	0.0	4.279892042861806E-6	0.23708033982228688	0.202529484675604
48-49	7.582542069270166E-4	0.0	0.0	4.279892042861806E-6	0.24666943794431878	0.211743378928545
50-51	8.281591102937596E-4	0.0	0.0	4.279892042861806E-6	0.2563776597282103	0.2207154592810643
52-53	8.916441755962097E-4	0.0	0.0	4.279892042861806E-6	0.2660623421058661	0.22992792690332434
54-55	9.558425562391367E-4	0.0	0.0	4.279892042861806E-6	0.2757077921397957	0.23919817306816302
56-57	0.001032880613010649	0.0	0.0	4.279892042861806E-6	0.28541458729300623	0.24829223034390388
58-59	0.0011170518231869314	0.0	0.0	4.279892042861806E-6	0.29511139603145015	0.25737915446624
60-61	0.0011883833572346283	0.0	0.0	4.279892042861806E-6	0.3049629941987775	0.2665445432760285
62-63	0.0012704146213894795	0.0	0.0	4.279892042861806E-6	0.31490019020696214	0.27590894706581015
64-65	0.00139025159858961	0.0	0.0	4.279892042861806E-6	0.3248580723600206	0.2851399608869226
66-67	0.0015150817831730795	0.0	0.0	4.279892042861806E-6	0.33471894362677423	0.2944744054324042
68-69	0.0016506116978637032	0.0	0.0	4.279892042861806E-6	0.3448815472825496	0.30423255929012916
70-71	0.0017718753057447878	0.0	0.0	4.279892042861806E-6	0.35507197023660353	0.3139286547132325
72-73	0.0019159650045211354	7.133153404769677E-7	0.0	4.279892042861806E-6	0.3652787994434885	0.32382376511632904
74-75	0.0020871606862356076	1.4266306809539354E-6	0.0	4.279892042861806E-6	0.3758487061586762	0.33359190538882066
76-77	0.002277615882142958	1.4266306809539354E-6	0.0	4.279892042861806E-6	0.38625027045351135	0.343196696448343
78-79	0.0025122966291598802	1.4266306809539354E-6	0.0	4.279892042861806E-6	0.3965961961517893	0.35259105948242464
80-81	0.0027519705835601415	1.4266306809539354E-6	0.0	4.279892042861806E-6	0.4071632496056151	0.3621188124851755
82-83	0.002997351060684218	1.4266306809539354E-6	0.0	4.279892042861806E-6	0.4180120626189293	0.3717828087179575
84-85	0.003256284529277358	1.4266306809539354E-6	0.0	4.279892042861806E-6	0.4286875400045076	0.38158590144213245
86-87	0.003580129693853901	1.4266306809539354E-6	0.0	4.279892042861806E-6	0.4394436220235598	0.3912834234959168
88-89	0.003916814534559029	1.4266306809539354E-6	0.0	5.706522723815742E-6	0.45009270674154045	0.40105156376840845
90-91	0.004286311880926099	1.4266306809539354E-6	0.0	7.133153404769677E-6	0.46062694768970425	0.41073909940742614
92-93	0.004672928795464616	1.4266306809539354E-6	0.0	7.133153404769677E-6	0.4714515079814423	0.42062992991847975
94-95	0.005174389479819924	1.4266306809539354E-6	0.0	7.133153404769677E-6	0.48227036175045646	0.4302939261512617
96-97	0.005765727897075331	1.4266306809539354E-6	0.0	7.133153404769677E-6	0.4931983527665636	0.43981454600060776
98-99	0.006409138334185555	1.4266306809539354E-6	0.0	7.133153404769677E-6	0.5041263437826707	0.44936726504027535
100-101	0.007149559657600647	1.4266306809539354E-6	0.0	8.559784085723612E-6	0.5151449258470184	0.4590284080116954
102-103	0.007936346478146743	2.853261361907871E-6	0.0	1.1413045447631483E-5	0.5263382701697831	0.46911326029535877
104-105	0.008816577608295321	2.853261361907871E-6	0.0	1.2839676128585418E-5	0.5375886797197857	0.47882718860197415
106-107	0.009805945985536874	2.853261361907871E-6	0.0	1.2839676128585418E-5	0.5487313786533765	0.48871801911302776
108-109	0.011037841578540599	2.853261361907871E-6	0.0	1.2839676128585418E-5	0.5599810748880387	0.4986673414820005
110-111	0.012413826870320669	2.853261361907871E-6	0.0	1.2839676128585418E-5	0.5709554314012768	0.5087593269190687
112-113	0.013963147789836643	4.279892042861806E-6	0.0	1.2839676128585418E-5	0.5824797540420228	0.5187564414158534
114-115	0.015668684768917075	4.279892042861806E-6	0.0	1.4266306809539355E-5	0.5940939544156687	0.5285645273474117
116-117	0.017592496242183453	4.279892042861806E-6	0.0	1.4266306809539355E-5	0.6055398123689621	0.538540242383982
118-119	0.01982374662719541	4.279892042861806E-6	0.0	1.4266306809539355E-5	0.6170776880011771	0.5483904139206285
120-121	0.022254011992200437	4.279892042861806E-6	0.0	1.4266306809539355E-5	0.6284871668721063	0.5582348789345511
122-123	0.02501810893654869	4.279892042861806E-6	0.0	1.4266306809539355E-5	0.6401826851945667	0.568040824920088
124-125	0.028185229048266425	4.279892042861806E-6	0.0	1.4266306809539355E-5	0.651939548636308	0.5778524774283487
126-127	0.0317197065603298	4.279892042861806E-6	0.0	1.4979622150016323E-5	0.6634210723566252	0.5876405905303737
128-129	0.03589830782484388	4.279892042861806E-6	0.0	1.569293749049329E-5	0.6751601029148547	0.5972874671949842
130-131	0.04066111435320859	4.279892042861806E-6	0.0	1.569293749049329E-5	0.6872579310893441	0.6070606006748591
132-133	0.04586546307732855	4.279892042861806E-6	0.0	1.569293749049329E-5	0.6991510377611165	0.6169721173307865
134-135	0.05166685674142773	4.279892042861806E-6	0.0	1.569293749049329E-5	0.7110548441629962	0.6269050334469284
136-137	0.058217231513027726	4.279892042861806E-6	0.0	1.569293749049329E-5	0.7229957429625806	0.6370248381822751
138-139	0.06571417574144064	4.279892042861806E-6	0.0	1.569293749049329E-5	0.7350243795490438	0.6468757230342619
>>END_MODULE
