##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065364_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79818903
Total Bases	11.9 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.877526104311407	32.0	32.0	32.0	32.0	32.0
2	31.302267659579336	32.0	32.0	32.0	32.0	32.0
3	34.56875354450812	37.0	32.0	37.0	32.0	37.0
4	35.47539410557923	37.0	37.0	37.0	32.0	37.0
5	35.84345542809577	37.0	37.0	37.0	37.0	37.0
6	39.38749673119411	41.0	41.0	41.0	37.0	41.0
7	39.53577071837231	41.0	41.0	41.0	37.0	41.0
8	39.571316596521	41.0	41.0	41.0	37.0	41.0
9	39.55211713445874	41.0	41.0	41.0	37.0	41.0
10-14	39.63898996707585	41.0	41.0	41.0	37.0	41.0
15-19	39.56784200855278	41.0	41.0	41.0	37.0	41.0
20-24	39.49231026640393	41.0	41.0	41.0	37.0	41.0
25-29	39.425956357731444	41.0	41.0	41.0	37.0	41.0
30-34	39.3574985088432	41.0	41.0	41.0	37.0	41.0
35-39	39.27825499180313	41.0	41.0	41.0	37.0	41.0
40-44	39.21030780390455	41.0	41.0	41.0	37.0	41.0
45-49	39.11799089245814	41.0	41.0	41.0	37.0	41.0
50-54	38.98982592381657	41.0	41.0	41.0	37.0	41.0
55-59	38.87253861657307	41.0	41.0	41.0	37.0	41.0
60-64	38.76628028075004	41.0	41.0	41.0	36.0	41.0
65-69	38.665887232251244	41.0	41.0	41.0	32.0	41.0
70-74	38.54504533594003	41.0	41.0	41.0	32.0	41.0
75-79	38.321840271345245	41.0	39.4	41.0	32.0	41.0
80-84	38.498605183787106	41.0	41.0	41.0	32.0	41.0
85-89	38.372287882733744	41.0	41.0	41.0	32.0	41.0
90-94	38.196016023923555	41.0	41.0	41.0	32.0	41.0
95-99	37.99962189658257	41.0	39.4	41.0	31.0	41.0
100-104	37.7882657169568	41.0	37.0	41.0	27.0	41.0
105-109	37.54555535547764	41.0	37.0	41.0	27.0	41.0
110-114	37.337632755990136	41.0	37.0	41.0	27.0	41.0
115-119	37.07573441093271	41.0	37.0	41.0	27.0	41.0
120-124	36.730019897667596	41.0	37.0	41.0	26.0	41.0
125-129	36.365145664805745	41.0	37.0	41.0	22.0	41.0
130-134	36.0022977138636	41.0	37.0	41.0	22.0	41.0
135-139	35.55386195924041	41.0	32.0	41.0	22.0	41.0
140-144	34.98662097122532	39.4	32.0	41.0	22.0	41.0
145-149	34.41276679284856	37.0	32.0	41.0	14.0	41.0
150	34.03425759935588	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	54.0
12	843.0
13	2191.0
14	6030.0
15	14354.0
16	24849.0
17	36113.0
18	52464.0
19	80748.0
20	128123.0
21	202354.0
22	294684.0
23	387745.0
24	479552.0
25	578093.0
26	685841.0
27	806965.0
28	942306.0
29	1085453.0
30	1233218.0
31	1374725.0
32	1514181.0
33	1682977.0
34	1945443.0
35	2399332.0
36	3189440.0
37	4592297.0
38	7361938.0
39	1.4659312E7
40	3.4057277E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	0.12628559964734282	51.33445944706817	32.426529565380186	16.1127253879043
2	0.1358959272116531	52.88041356363762	34.06127769309419	12.92241281605654
3	0.19834770632557114	48.383300690705205	35.36472851430194	16.053623088667283
4	0.24386970603334346	47.39711071803925	35.6190227792136	16.739996796713804
5	0.2571414067134721	47.75048407185842	35.54110007866825	16.45127444275985
6	0.2727709877569147	47.43958069124886	35.6394948816821	16.648153439312132
7	0.27375855305203745	48.07972865967193	35.21635408646947	16.43015870080656
8	0.25452202050964695	46.68102313051361	36.41832181693312	16.64613303204362
9	0.26631275232110957	46.99685534059326	36.30231221875847	16.434519688327157
10-14	0.26538598589983947	47.459227374466195	35.98594343844253	16.289443201191425
15-19	0.28310803602207857	47.09735158403364	36.19532807745208	16.424212302492204
20-24	0.31129891079684113	47.275697914040364	35.796131877603266	16.61687129755953
25-29	0.318276347880363	47.25872712432912	35.68882185963913	16.73417466815139
30-34	0.3075859073869085	47.39121506618996	35.53494693415449	16.766252092268648
35-39	0.31500588918399575	47.346504330735875	35.55371390409371	16.78477587598641
40-44	0.31181241267750004	47.40067611254112	35.49789646214103	16.78961501264035
45-49	0.3141980680797201	47.36679081985331	35.52227798719708	16.796733124869892
50-54	0.31586037171207176	47.39097699233367	35.49951724583898	16.793645390115277
55-59	0.32242739268543064	47.37643070853709	35.501770538781315	16.799371359996158
60-64	0.32620859635475924	47.359156375198815	35.49107026913288	16.823564759313548
65-69	0.3319647935294916	47.34085426335545	35.49887324668711	16.828307696427945
70-74	0.34084636448101374	47.31733095792676	35.50180667739514	16.840016000197092
75-79	0.34668567395672717	47.30759532067554	35.50056415102596	16.845154854341764
80-84	0.35004270548820043	47.2886891678882	35.511453675103624	16.84981445151997
85-89	0.35677734785714865	47.27363091245361	35.507632190487	16.861959549202236
90-94	0.369868419646476	47.238527723174194	35.50927110968131	16.882332747498026
95-99	0.378784410682788	47.222206979264435	35.49636993178189	16.902638678270886
100-104	0.3855289058053229	47.19342210034475	35.49484250325741	16.926206490592524
105-109	0.3956158319069352	47.1552780870674	35.491285707841314	16.957820373184358
110-114	0.3972718209557119	47.13504983295304	35.50650009180299	16.961178254288264
115-119	0.42088749467230346	47.11376582070131	35.47154707788146	16.993799606744926
120-124	0.42883474581606384	47.074955199261666	35.47855928377652	17.01765077114575
125-129	0.44730800804448945	47.057195446622465	35.45578700796697	17.039709537366083
130-134	0.4560469743515432	47.02711180867013	35.453004893844	17.063836323134332
135-139	0.48202041414002816	46.98586622155071	35.43217558316449	17.099937781144778
140-144	0.5061910016858808	46.94054964528344	35.41641007599494	17.136849277035736
145-149	0.5186189248789063	46.880506739630775	35.407089394538374	17.193784940951947
150	0.5355773011764089	46.852065292762276	35.37238342348897	17.239973982572344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5459.0
1	7001.0
2	13180.5
3	29807.5
4	69512.0
5	156445.0
6	325659.0
7	620662.0
8	1087613.5
9	1745917.0
10	2578503.5
11	3521526.5
12	4470267.5
13	5313170.0
14	5942998.5
15	6296574.0
16	6358371.5
17	6144108.5
18	5709226.0
19	5119632.0
20	4450255.0
21	3767709.0
22	3122048.5
23	2550626.0
24	2058284.0
25	1648658.0
26	1315055.5
27	1040892.0
28	820984.5
29	650086.0
30	519311.5
31	415205.5
32	329125.0
33	260541.5
34	207819.0
35	166456.5
36	134922.0
37	111014.5
38	92000.0
39	76508.5
40	63888.0
41	54060.5
42	46268.0
43	39703.5
44	34008.5
45	29550.0
46	25774.5
47	22592.0
48	20255.5
49	19044.0
50	18163.0
51	17031.0
52	15656.5
53	13810.5
54	12303.5
55	11188.5
56	10198.5
57	9204.0
58	8390.5
59	7668.5
60	7014.0
61	6520.5
62	6015.5
63	5522.5
64	5083.5
65	4636.0
66	4187.5
67	3827.5
68	3451.0
69	3086.0
70	2834.0
71	2625.0
72	2370.0
73	2137.0
74	1981.5
75	1801.5
76	1643.0
77	1544.5
78	1396.0
79	1234.5
80	1175.5
81	1150.0
82	1041.5
83	964.0
84	885.0
85	825.5
86	770.5
87	688.5
88	692.0
89	705.5
90	691.0
91	658.5
92	609.0
93	602.0
94	590.0
95	596.0
96	604.5
97	647.5
98	819.5
99	1196.0
100	2824.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9432364661789452
2	0.005198016815640776
3	0.001871737074612514
4	0.0010912202088269743
5	2.192463106139156E-4
6	3.7209231001333106E-4
7	3.382657363757555E-5
8	3.3701290031510456E-4
9	9.020419636686814E-4
10-14	6.52727587599143E-4
15-19	7.649816986334678E-4
20-24	7.146176889952997E-4
25-29	2.71364290736995E-4
30-34	2.024583074011929E-4
35-39	2.565808252213138E-4
40-44	0.0026707959140956873
45-49	1.799072583094759E-4
50-54	0.002095744162256903
55-59	4.693123883198445E-4
60-64	5.081503062000237E-4
65-69	0.0037953415621359765
70-74	0.002363099377599815
75-79	0.003144618512233875
80-84	4.465107720159972E-4
85-89	0.002375627738206324
90-94	8.519285212426435E-4
95-99	7.810180002097999E-4
100-104	5.665324666263579E-4
105-109	0.002665534002640953
110-114	1.9218505170385516E-4
115-119	0.003149629856476479
120-124	5.813159321420391E-4
125-129	0.002729428641734152
130-134	6.296754040831657E-4
135-139	0.0020290932838302727
140-144	0.003837436853773848
145-149	0.0012891683064098238
150	0.011678937757388121
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.9818903E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.05019943900247
#Duplication Level	Percentage of total
1	75.24886324133736
2	15.49034732739775
3	3.864050257045736
4	1.293257590663632
5	0.7212061341821886
6	0.4761292331425242
7	0.38117032669388373
8	0.2649986087324543
9	0.22902274830344838
>10	1.6753619146421366
>50	0.14035383130813758
>100	0.15239232467696384
>500	0.025122111718124347
>1k	0.029992474473623555
>5k	0.007731875682105436
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.2551049091717035E-5	0.0	0.0	0.0	0.16079399136818506	0.0
2	2.2551049091717035E-5	0.0	0.0	0.0	0.20688457720347272	1.2528360606509463E-6
3	2.2551049091717035E-5	0.0	0.0	0.0	0.24079884936529383	1.2528360606509463E-6
4	2.7562393334320817E-5	0.0	0.0	0.0	0.2778226606296506	1.2528360606509463E-6
5	2.7562393334320817E-5	0.0	0.0	0.0	0.3156370114482781	1.2528360606509463E-6
6	2.7562393334320817E-5	0.0	0.0	0.0	0.35381593756055507	3.758508181952839E-6
7	2.7562393334320817E-5	0.0	0.0	0.0	0.39166912629706274	5.011344242603785E-6
8	2.8815229394971766E-5	0.0	0.0	1.2528360606509463E-6	0.4277984126141147	5.011344242603785E-6
9	3.006806545562271E-5	0.0	0.0	1.2528360606509463E-6	0.4656340616457733	5.011344242603785E-6
10-11	3.1947319546599126E-5	0.0	0.0	1.2528360606509463E-6	0.521715388646722	6.264180303254732E-6
12-13	3.946433591050481E-5	0.0	0.0	1.2528360606509463E-6	0.5939188364941573	8.769852424556624E-6
14-15	4.510209818343407E-5	0.0	0.0	1.2528360606509463E-6	0.6652904011973204	1.3781196667160409E-5
16-17	6.452105712352374E-5	0.0	0.0	1.2528360606509463E-6	0.7370616206038312	1.5034032727811355E-5
18-19	9.020419636686814E-5	0.0	0.0	1.2528360606509463E-6	0.8077929860800016	1.6286868788462304E-5
20-21	1.2966853227737294E-4	0.0	0.0	1.2528360606509463E-6	0.878601400973902	1.8792540909764193E-5
22-23	1.8291406485503817E-4	0.0	0.0	1.2528360606509463E-6	0.9496403377029624	1.8792540909764193E-5
24-25	2.493143760695383E-4	0.0	0.0	1.2528360606509463E-6	1.0208747168574843	1.8792540909764193E-5
26-27	3.413978265273829E-4	0.0	0.0	1.2528360606509463E-6	1.0920101219632146	1.8792540909764193E-5
28-29	4.4225112940978405E-4	0.0	0.0	3.758508181952839E-6	1.163499453256079	2.0045376970415138E-5
30-31	5.70666825626506E-4	0.0	0.0	3.758508181952839E-6	1.2349637278277301	2.192463106139156E-5
32-33	7.310298413898272E-4	0.0	0.0	3.758508181952839E-6	1.3073845427316884	2.2551049091717035E-5
34-35	9.10811816093238E-4	0.0	0.0	3.758508181952839E-6	1.3791200563104709	2.380388515236798E-5
36-37	0.0010636578154926534	0.0	0.0	5.011344242603785E-6	1.451086718142443	2.380388515236798E-5
38-39	0.001222141577164998	0.0	0.0	5.011344242603785E-6	1.5231078783430536	2.8188811364646295E-5
40-41	0.0013806253388373429	0.0	0.0	5.011344242603785E-6	1.595261212748063	3.570582772855197E-5
42-43	0.0015447468627826167	0.0	0.0	5.011344242603785E-6	1.6672974069814015	4.259642606213217E-5
44-45	0.0017270345096073295	0.0	0.0	5.011344242603785E-6	1.7393962430177723	4.823418833506143E-5
46-47	0.0019168391727959477	0.0	0.0	5.011344242603785E-6	1.8107878530979058	5.324553257766522E-5
48-49	0.0021191721965910758	0.0	0.0	5.011344242603785E-6	1.8829568479536734	6.0762548941570895E-5
50-51	0.0023240108925075055	0.0	0.0	5.011344242603785E-6	1.9550174724901943	6.76531472751511E-5
52-53	0.0025344873506968645	0.0	0.0	5.011344242603785E-6	2.027113802854444	8.206076197263699E-5
54-55	0.0027825488907057515	0.0	0.0	6.890598333580205E-6	2.099187582169602	8.707210621524077E-5
56-57	0.0030093122176835727	0.0	0.0	8.769852424556624E-6	2.1704576671518527	9.584195863979739E-5
58-59	0.0032730342084505972	0.0	0.0	9.396270454882098E-6	2.2417465446750127	1.0774390121598139E-4
60-61	0.0035355033631569704	0.0	0.0	1.002268848520757E-5	2.31268337526513	1.1964584379216538E-4
62-63	0.0037998517719543203	0.0	0.0	1.002268848520757E-5	2.3833495932661464	1.2778927818639653E-4
64-65	0.004086751229843387	0.0	0.0	1.1275524545858517E-5	2.4545351118143026	1.421968928838824E-4
66-67	0.004396828154854496	0.0	0.0	1.1275524545858517E-5	2.524920694537734	1.5911017970267018E-4
68-69	0.00467307850622803	0.0	0.0	1.1275524545858517E-5	2.595306903679195	1.791555566730853E-4
70-71	0.004943691095328634	0.0	0.0	1.1901942576183991E-5	2.66550831950171	1.9857451561317499E-4
72-73	0.005241239659733233	0.0	0.0	1.2528360606509464E-5	2.736108137191512	2.192463106139156E-4
74-75	0.00559328659277615	0.0	0.0	1.2528360606509464E-5	2.8077133558199865	2.386652695540053E-4
76-77	0.00595034487006167	0.0	0.0	1.3154778636834936E-5	2.878710322541015	2.80635277585812E-4
78-79	0.006292369114619377	0.0	0.0	1.3781196667160409E-5	2.9493045024685447	3.200996134963168E-4
80-81	0.006669472768875313	0.0	0.0	1.3781196667160409E-5	3.0194902578403013	3.4452991667901025E-4
82-83	0.007056599111616455	0.0	0.0	1.3781196667160409E-5	3.089956648489644	3.7209231001333106E-4
84-85	0.007471914265722244	0.0	0.0	1.3781196667160409E-5	3.160742512334453	3.9526977713537354E-4
86-87	0.007893493600131286	0.0	0.0	1.5034032727811355E-5	3.2311237101316714	4.3285485895490193E-4
88-89	0.008371450557269624	0.0	0.0	1.5034032727811355E-5	3.301055766201147	4.6480217850150107E-4
90-91	0.008856924530771866	0.0	0.0	1.5034032727811355E-5	3.3710373092950174	4.986287521390766E-4
92-93	0.009392511946700145	0.0	0.0	1.5034032727811355E-5	3.441219932576623	5.312024897160012E-4
94-95	0.009968816534599578	0.0	0.0	1.5034032727811355E-5	3.5108964100897255	5.73172497747808E-4
96-97	0.010578321278106265	0.0	0.0	1.6286868788462304E-5	3.5806962919548018	6.095047435066853E-4
98-99	0.01124608289843322	0.0	0.0	1.753970484911325E-5	3.6502368367553233	6.546068416901195E-4
100-101	0.011995278862702484	0.0	0.0	1.9418958940089667E-5	3.7191797787549143	6.928183415399734E-4
102-103	0.012788324089094534	0.0	0.0	2.004537697041514E-5	3.7888374637271074	7.397996938143837E-4
104-105	0.013618954397306112	0.0	0.0	2.004537697041514E-5	3.858961203713862	7.874074641191197E-4
106-107	0.014591155180371247	0.0	0.0	2.004537697041514E-5	3.9288788521686397	8.362680704845066E-4
108-109	0.015546442676617592	0.0	0.0	2.004537697041514E-5	3.997452207530339	9.014155456383559E-4
110-111	0.01671032737696232	0.0	0.0	2.1298213031066086E-5	4.0662210053175	9.628045126102523E-4
112-113	0.017959404929431315	0.0	0.0	2.1298213031066086E-5	4.134995440866934	0.0010266991517034504
114-115	0.019398913563119253	0.0	0.0	2.1298213031066086E-5	4.203771755670458	0.0010956051350392527
116-117	0.020973102073327168	0.0	0.0	2.2551049091717035E-5	4.271740617632894	0.0011538620118595215
118-119	0.0227370952467237	0.0	0.0	2.2551049091717035E-5	4.340380999723838	0.0012133717247404415
120-121	0.02460507381315426	0.0	0.0	2.2551049091717035E-5	4.409206801551758	0.0012916739785311256
122-123	0.026673506149288973	0.0	0.0	2.2551049091717035E-5	4.47842223539454	0.0013549421995939985
124-125	0.0290275851072521	0.0	0.0	2.5056721213018928E-5	4.547194791689883	0.0014319916173240318
126-127	0.03172619398189424	0.0	0.0	2.56831392433444E-5	4.616269907893873	0.0015096674530843904
128-129	0.034679754994878846	0.0	0.0	2.6309557273669873E-5	4.685248555721193	0.0015904753789963764
130-131	0.03800854040802841	0.0	0.0	2.6309557273669873E-5	4.754152659802903	0.0016769210671812916
132-133	0.04171192380331261	0.0	0.0	2.7562393334320817E-5	4.822513659452323	0.001772763025821089
134-135	0.04584189787724845	0.0	0.0	2.818881136464629E-5	4.8902883318253565	0.0018723634926428394
136-137	0.05050119518680932	0.0	0.0	2.8815229394971766E-5	4.958256567369762	0.001956929926736778
138-139	0.05575621604320971	0.0	0.0	2.9441647425297236E-5	5.026947689321162	0.0020790814426502454
>>END_MODULE
