##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065364_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79818903
Total Bases	11.9 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218419664324376	32.0	32.0	32.0	32.0	32.0
2	31.46841245112076	32.0	32.0	32.0	32.0	32.0
3	35.08342335900056	37.0	32.0	37.0	32.0	37.0
4	35.79455058408909	37.0	37.0	37.0	32.0	37.0
5	36.17117183381987	37.0	37.0	37.0	37.0	37.0
6	39.66450496068582	41.0	41.0	41.0	37.0	41.0
7	39.83349474246721	41.0	41.0	41.0	37.0	41.0
8	39.94332477859286	41.0	41.0	41.0	37.0	41.0
9	39.984459157500574	41.0	41.0	41.0	37.0	41.0
10-14	40.00898066213714	41.0	41.0	41.0	37.0	41.0
15-19	39.98470437510273	41.0	41.0	41.0	37.0	41.0
20-24	39.95340174244189	41.0	41.0	41.0	37.0	41.0
25-29	39.82094180372286	41.0	41.0	41.0	37.0	41.0
30-34	39.72917494493754	41.0	41.0	41.0	37.0	41.0
35-39	39.67802607109246	41.0	41.0	41.0	37.0	41.0
40-44	39.60399777481282	41.0	41.0	41.0	37.0	41.0
45-49	39.55512144034352	41.0	41.0	41.0	37.0	41.0
50-54	39.478511986064255	41.0	41.0	41.0	37.0	41.0
55-59	39.40067246727257	41.0	41.0	41.0	37.0	41.0
60-64	39.30449126818994	41.0	41.0	41.0	37.0	41.0
65-69	39.19123557736693	41.0	41.0	41.0	37.0	41.0
70-74	39.109024307186985	41.0	41.0	41.0	37.0	41.0
75-79	38.935867605196236	41.0	40.2	41.0	36.0	41.0
80-84	39.16890280489071	41.0	41.0	41.0	37.0	41.0
85-89	39.078737298607074	41.0	41.0	41.0	37.0	41.0
90-94	38.97456515782984	41.0	41.0	41.0	37.0	41.0
95-99	38.84603503257868	41.0	41.0	41.0	37.0	41.0
100-104	38.71446060339868	41.0	41.0	41.0	32.0	41.0
105-109	38.583143494217154	41.0	41.0	41.0	32.0	41.0
110-114	38.43653744026024	41.0	41.0	41.0	32.0	41.0
115-119	38.28023685817882	41.0	37.0	41.0	32.0	41.0
120-124	38.042421347234	41.0	37.0	41.0	32.0	41.0
125-129	37.85941743899938	41.0	37.0	41.0	32.0	41.0
130-134	37.62807091698566	41.0	37.0	41.0	28.0	41.0
135-139	37.34600061842494	41.0	37.0	41.0	27.0	41.0
140-144	37.09748366499098	41.0	37.0	41.0	27.0	41.0
145-149	36.83215121360412	41.0	37.0	41.0	27.0	41.0
150	36.6640489408881	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	13.0
13	13.0
14	23.0
15	94.0
16	388.0
17	1223.0
18	2808.0
19	5028.0
20	8803.0
21	14795.0
22	25074.0
23	41865.0
24	70442.0
25	114301.0
26	177747.0
27	266302.0
28	385564.0
29	536673.0
30	727420.0
31	958484.0
32	1243110.0
33	1588651.0
34	2030140.0
35	2607303.0
36	3442721.0
37	4811623.0
38	7590455.0
39	1.5644877E7
40	3.7522963E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.927889335574527	40.94212356636286	43.75496051752164	0.37502658054098004
2	17.030202165252938	40.63799428547581	42.08615099805972	0.24565255121152532
3	15.069718073891744	37.712758816889696	46.916756072905876	0.3007670363126831
4	15.890182161023759	36.7778205950522	47.01927995210346	0.3127172918205909
5	16.31448630658329	36.58090866019544	46.796765673414484	0.3078393598067866
6	16.43000638332981	36.12612249967091	47.12167150806734	0.32219960893194105
7	16.849617875867484	36.28169246373787	46.56471778621217	0.3039718741824752
8	16.109258903964157	35.742607332304786	47.837178534413525	0.3109552293175327
9	16.449680707263315	35.606801403665024	47.63283778942241	0.31068009964925664
10-14	16.351403445085495	36.21857507012417	47.13349848360845	0.2965230011818885
15-19	16.333487355794	35.83931805742029	47.52839027809507	0.29880430869063834
20-24	16.519301853160457	35.81860322893873	47.359440826801894	0.30265409109892355
25-29	16.649217031582523	35.61759667011265	47.420714311353066	0.3124719869517688
30-34	16.754967901139327	35.5782878320957	47.351990420221135	0.31475384654384575
35-39	16.78888006243593	35.4873434419286	47.40654111409704	0.3172353815384286
40-44	16.795851305334335	35.50710842964252	47.37731305139092	0.3197272136322303
45-49	16.794178951834795	35.48795214848728	47.39894928739767	0.3189196122802523
50-54	16.794977699104376	35.50535697651071	47.37845803849965	0.3212072858852564
55-59	16.797650953245416	35.49577484628776	47.383438475291236	0.32313572517559563
60-64	16.779124720957146	35.501400297399655	47.39379653459572	0.32567844704748095
65-69	16.79621408068042	35.50475011193836	47.37118709417285	0.3278487132083588
70-74	16.781914607995475	35.51065727636453	47.37599927571643	0.331428839923564
75-79	16.786471102611884	35.50889502981837	47.379396174437424	0.325237693132314
80-84	16.785506216360158	35.489868562574856	47.39887426863827	0.3257509524267163
85-89	16.797717629801802	35.49512288748358	47.36568540822044	0.34147407449417794
90-94	16.773605643240643	35.488223926894655	47.39964067806288	0.3385297518018196
95-99	16.784135682539755	35.50313461175379	47.367643488801946	0.3450862169045187
100-104	16.758633818394962	35.500725957945534	47.395153881349586	0.34548634230991515
105-109	16.76401069215141	35.50504687763785	47.376533239750586	0.35440919046015606
110-114	16.764858647862564	35.498092036239875	47.384956704704	0.35209261119356616
115-119	16.744931385867876	35.4942841668879	47.40092518186678	0.35985926537744184
120-124	16.74035496287599	35.48784370753593	47.398946343434325	0.37285498615376156
125-129	16.739547847553247	35.49847909807553	47.38219421564897	0.3797788387222545
130-134	16.725915453256896	35.49192628733099	47.389586714371525	0.3925715450405911
135-139	16.73091770632572	35.47113679561848	47.38922959002284	0.4087159080329571
140-144	16.718851767549246	35.47330759602472	47.39235102778061	0.41548960864542006
145-149	16.71132933240361	35.457693911737195	47.40521671401972	0.42576004183947347
150	16.702402891065898	35.47928884746391	47.39097956186824	0.4273286996019442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5570.0
1	7820.0
2	15084.5
3	32667.0
4	72350.0
5	157010.0
6	320983.5
7	605635.0
8	1058743.5
9	1698164.0
10	2509617.0
11	3439041.5
12	4381965.0
13	5225858.5
14	5872509.0
15	6257242.0
16	6357857.0
17	6183083.5
18	5775682.0
19	5203460.5
20	4543104.0
21	3858507.5
22	3200824.0
23	2612457.5
24	2108923.5
25	1690387.5
26	1348281.5
27	1070909.5
28	847881.0
29	673331.5
30	538642.0
31	430436.0
32	340437.5
33	266758.5
34	209437.5
35	166239.0
36	132605.0
37	106141.0
38	85915.0
39	69413.5
40	56058.5
41	45397.0
42	36880.5
43	30000.5
44	24258.0
45	19750.5
46	16043.0
47	13215.0
48	11007.5
49	10255.0
50	10852.5
51	10530.5
52	8955.0
53	7196.0
54	5955.5
55	5007.5
56	4207.5
57	3653.0
58	3336.5
59	3026.0
60	2557.5
61	2107.0
62	1682.0
63	1291.0
64	1007.0
65	803.5
66	642.0
67	513.0
68	440.0
69	366.5
70	295.0
71	241.0
72	207.0
73	162.5
74	116.5
75	99.5
76	106.0
77	95.5
78	105.5
79	113.5
80	90.5
81	84.5
82	76.5
83	59.5
84	47.0
85	42.5
86	41.5
87	35.0
88	24.5
89	19.5
90	14.5
91	10.5
92	8.0
93	7.5
94	10.0
95	13.0
96	10.0
97	9.0
98	24.5
99	66.0
100	764.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7543551431670265
2	0.0011075070776154364
3	8.118377673018132E-4
4	0.00861825926121786
5	0.0
6	7.517016363905677E-5
7	0.0012428133721657387
8	2.1298213031066086E-5
9	1.8040839273373628E-4
10-14	2.563302580091836E-4
15-19	3.0243462504113845E-4
20-24	3.209765987387724E-4
25-29	0.0014883692400533242
30-34	3.452816183154008E-4
35-39	9.483968979127664E-4
40-44	6.091288926884901E-4
45-49	0.0011533608774352611
50-54	6.755292039029903E-4
55-59	3.0669426764735164E-4
60-64	9.86232546944425E-4
65-69	0.0010877122678571516
70-74	5.53502971595588E-4
75-79	3.6933607067989895E-4
80-84	0.0017832868487305568
85-89	0.001122791677555378
90-94	5.677853026870088E-4
95-99	0.00182713611085334
100-104	0.003049152404412273
105-109	2.5733252685770436E-4
110-114	3.7710365425593485E-4
115-119	5.981039353547617E-4
120-124	3.2248000201155356E-4
125-129	0.0014194632567175222
130-134	0.0010533845597953157
135-139	0.0016412152394527394
140-144	0.0012205128902861519
145-149	0.001822876468247127
150	0.005593913010806475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.9818903E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.10375926011959
#Duplication Level	Percentage of total
1	73.77261936246599
2	16.292011583810414
3	4.102382225789903
4	1.39511436898536
5	0.7090662193082033
6	0.4800599537933418
7	0.4182176679148215
8	0.27298671231246713
9	0.25309254668867814
>10	1.9442215182301932
>50	0.144424311765803
>100	0.1265293335919262
>500	0.042826357100670454
>1k	0.02652122111210804
>5k	0.019926617130169473
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.5056721213018928E-5	0.0	0.0	0.0	0.0037484854934676315	0.0014770937155074656
2	2.5056721213018928E-5	0.0	0.0	0.0	0.012692482130454737	0.004983781849269464
3	2.6309557273669873E-5	0.0	0.0	0.0	0.020346057624971367	0.007499476659056564
4	3.006806545562271E-5	0.0	0.0	0.0	0.028162501807372623	0.010403550647645457
5	3.132090151627366E-5	0.0	0.0	0.0	0.03577222803976647	0.0137686683065539
6	3.132090151627366E-5	0.0	0.0	0.0	0.043508490714286065	0.017574784258811475
7	3.132090151627366E-5	0.0	0.0	0.0	0.05102550707819174	0.021276914818035023
8	3.257373757692461E-5	0.0	0.0	0.0	0.05821302755814622	0.024844991918768915
9	3.382657363757555E-5	0.0	0.0	0.0	0.06532287220234034	0.028806459542547208
10-11	3.8837917880179335E-5	0.0	0.0	0.0	0.07577403062029053	0.03496540161670726
12-13	4.19700080318067E-5	0.0	0.0	0.0	0.08864504188938804	0.04354106445186298
14-15	4.572851621375954E-5	0.0	0.0	0.0	0.10102556784074068	0.05243870615460601
16-17	5.073986045636332E-5	0.0	0.0	0.0	0.11280160039282924	0.06164391911023884
18-19	5.8256876820269E-5	0.0	0.0	0.0	0.12434022051142447	0.0709975931390588
20-21	6.827956530547658E-5	0.0	0.0	0.0	0.1355205295166735	0.08020531176681293
22-23	8.64456881849153E-5	0.0	0.0	0.0	0.14614457931099353	0.08913427437107223
24-25	1.0523822909467949E-4	0.0	0.0	0.0	0.15643036336893779	0.09815469400775903
26-27	1.309213683380239E-4	0.0	0.0	0.0	0.1667111360826395	0.10703604884171361
28-29	1.5535167152071734E-4	0.0	0.0	0.0	0.17696747849315844	0.11606398549476432
30-31	1.8980466318861838E-4	0.0	0.0	0.0	0.1873929537718653	0.1250787673691782
32-33	2.4618228591791094E-4	0.0	0.0	0.0	0.1976060733382918	0.13420567807102035
34-35	3.0631841682915635E-4	0.0	0.0	0.0	0.20788434037987216	0.1433056527975585
36-37	3.464091707699867E-4	0.0	0.0	0.0	0.2183956349287336	0.15232231392606335
38-39	3.827414165288641E-4	0.0	0.0	0.0	0.2288950275350189	0.16123874816971615
40-41	4.2533784259099624E-4	0.0	0.0	0.0	0.23927915922372422	0.1700637253809414
42-43	4.760777030473596E-4	0.0	0.0	1.2528360606509463E-6	0.24976978197758493	0.17896387275580572
44-45	5.393459241102323E-4	0.0	0.0	1.2528360606509463E-6	0.26013010978113793	0.188025635982494
46-47	5.857008583543174E-4	0.0	0.0	1.8792540909764194E-6	0.2707228386739417	0.19688443976735687
48-49	6.452105712352373E-4	0.0	0.0	3.758508181952839E-6	0.28144210400887115	0.20595246717434842
50-51	7.116108824497375E-4	0.0	0.0	3.758508181952839E-6	0.2921131351554656	0.21511821579407073
52-53	7.667356691183791E-4	0.0	0.0	3.758508181952839E-6	0.3029301217031259	0.2241849903650016
54-55	8.350152344238557E-4	0.0	0.0	3.758508181952839E-6	0.3138291690127588	0.23335261823881492
56-57	9.058004718506342E-4	0.0	0.0	3.758508181952839E-6	0.3245070907576868	0.24263863912035977
58-59	9.859819797322948E-4	0.0	0.0	3.758508181952839E-6	0.3356723657302081	0.25182631237114345
60-61	0.001068669159735257	0.0	0.0	3.758508181952839E-6	0.3468132103995466	0.261005842187533
62-63	0.001146344995495616	0.0	0.0	3.758508181952839E-6	0.35773856726645314	0.27033070098695794
64-65	0.0012359227738321585	0.0	0.0	3.758508181952839E-6	0.36887314775548846	0.2799280516295745
66-67	0.0013298854783809796	0.0	0.0	3.758508181952839E-6	0.38013614394074047	0.2895435683950705
68-69	0.0014194632567175222	0.0	0.0	3.758508181952839E-6	0.39135466444583933	0.29925179502905475
70-71	0.0015102938711147157	0.0	0.0	3.758508181952839E-6	0.4029834887608014	0.30899071614652485
72-73	0.001624301952633952	0.0	0.0	3.758508181952839E-6	0.4146599208460683	0.3189952385088529
74-75	0.0017527176488506737	0.0	0.0	3.758508181952839E-6	0.42626932620209024	0.32887385084708565
76-77	0.0018805069270370705	0.0	0.0	3.758508181952839E-6	0.4380766796556951	0.3387261536280447
78-79	0.0020220774018906273	0.0	0.0	3.758508181952839E-6	0.4500256035841535	0.34837487054914795
80-81	0.002173044147199066	0.0	0.0	3.758508181952839E-6	0.46189309316866956	0.3580574140438888
82-83	0.002335286417053364	0.0	0.0	4.384926212278312E-6	0.4737774960400044	0.3678539656201489
84-85	0.0025094306294838453	0.0	0.0	5.011344242603785E-6	0.48562744591967144	0.37779647259747484
86-87	0.0027054994729757186	0.0	0.0	5.011344242603785E-6	0.49751811297130955	0.38766318299313135
88-89	0.002947923250711677	0.0	0.0	5.011344242603785E-6	0.5096242678253797	0.3974716365119676
90-91	0.0032229207660245595	0.0	0.0	5.011344242603785E-6	0.521655878933841	0.40750309484959973
92-93	0.0035167108222472062	0.0	0.0	5.011344242603785E-6	0.5336874900423023	0.41751012288404915
94-95	0.003856855812713938	0.0	0.0	5.011344242603785E-6	0.5460316837478961	0.42756225301668205
96-97	0.00424272931939443	0.0	0.0	5.011344242603785E-6	0.5584767307563723	0.4375373337315849
98-99	0.004691244629107468	0.0	0.0	5.011344242603785E-6	0.5705979196431703	0.4475230635530032
100-101	0.005206160250035008	0.0	0.0	5.011344242603785E-6	0.5830166570943728	0.45752946516942233
102-103	0.005769310059297608	0.0	0.0	5.011344242603785E-6	0.5955744593483074	0.4676116633675108
104-105	0.006365033606137133	0.0	0.0	5.011344242603785E-6	0.6079587438078421	0.4776807067869625
106-107	0.007095437029496634	0.0	0.0	5.011344242603785E-6	0.6206330598154174	0.48782116186187124
108-109	0.007838368813462646	0.0	0.0	5.637762272929259E-6	0.6330361368158618	0.4981107044279975
110-111	0.00876358824425337	0.0	0.0	6.264180303254732E-6	0.6456283920614645	0.5084578774529136
112-113	0.0097652306747438	0.0	0.0	6.264180303254732E-6	0.6582544738807047	0.5187518049452522
114-115	0.010971711801150662	0.0	0.0	6.264180303254732E-6	0.6709312955604012	0.5289549018231934
116-117	0.012309740713925873	0.0	0.0	6.264180303254732E-6	0.68365071366616	0.539031462259009
118-119	0.013850729068526538	0.0	0.0	6.264180303254732E-6	0.6964027055094957	0.5492784083990732
120-121	0.015489438635857975	0.0	0.0	6.264180303254732E-6	0.7090832856973742	0.5594746146786809
122-123	0.0173417567515304	0.0	0.0	6.264180303254732E-6	0.7221390902854177	0.5697879611299594
124-125	0.01945591760387887	0.0	0.0	7.517016363905678E-6	0.7351692117342179	0.5802835952280627
126-127	0.021946555692452952	0.0	0.0	7.517016363905678E-6	0.7481417027242281	0.5906664740807074
128-129	0.0246601985998229	0.0	0.0	8.769852424556624E-6	0.7611517787960579	0.6006885361478846
130-131	0.027692688284628515	0.0	0.0	8.769852424556624E-6	0.7743209650475903	0.6105633899779355
132-133	0.031131723271115366	0.0	0.0	9.396270454882098E-6	0.7877169146261005	0.6207733774542104
134-135	0.035077530444135516	1.2528360606509463E-6	0.0	1.1275524545858517E-5	0.8009932183608186	0.6309695837338181
136-137	0.03951946069717345	1.2528360606509463E-6	0.0	1.1275524545858517E-5	0.8141304572928044	0.6411219407513029
138-139	0.04453706912008049	1.2528360606509463E-6	0.0	1.1901942576183991E-5	0.8273359757900958	0.6515774840954655
>>END_MODULE
