SUMMARISING RUN PARAMETERS
==========================
Input filename: SRR10065364_1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.10
Cutadapt version: 4.2
Python version: could not detect
Number of cores used for trimming: 2
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Illumina adapter for trimming (count: 437). Second best hit was smallRNA (count: 0)
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed


This is cutadapt 4.2 with Python 3.10.8
Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR10065364_1.fastq.gz
Processing single-end reads on 2 cores ...
Finished in 1874.163 s (23.480 µs/read; 2.56 M reads/minute).

=== Summary ===

Total reads processed:              79,818,903
Reads with adapters:                33,382,849 (41.8%)
Reads written (passing filters):    79,818,903 (100.0%)

Total basepairs processed: 11,972,835,450 bp
Quality-trimmed:              22,705,178 bp (0.2%)
Total written (filtered):  11,906,035,988 bp (99.4%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 33382849 times

Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 41.9%
  C: 0.1%
  G: 12.8%
  T: 45.2%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	26726955	19954725.8	0	26726955
2	4343650	4988681.4	0	4343650
3	1630752	1247170.4	0	1630752
4	616955	311792.6	0	616955
5	4668	77948.1	0	4668
6	4647	19487.0	0	4647
7	3189	4871.8	0	3189
8	2874	1217.9	0	2874
9	3623	304.5	0	2610 1013
10	4144	76.1	1	2656 1488
11	2724	19.0	1	2278 446
12	2926	4.8	1	2667 259
13	1893	1.2	1	1803 90
14	1979	1.2	1	1853 126
15	1708	1.2	1	1634 74
16	1767	1.2	1	1672 95
17	1541	1.2	1	1470 71
18	1608	1.2	1	1517 91
19	1355	1.2	1	1279 76
20	1291	1.2	1	1228 63
21	1154	1.2	1	1113 41
22	1195	1.2	1	1132 63
23	1111	1.2	1	1051 60
24	1256	1.2	1	1186 70
25	1027	1.2	1	978 49
26	921	1.2	1	870 51
27	811	1.2	1	762 49
28	774	1.2	1	739 35
29	759	1.2	1	721 38
30	786	1.2	1	736 50
31	644	1.2	1	612 32
32	600	1.2	1	569 31
33	650	1.2	1	625 25
34	583	1.2	1	550 33
35	587	1.2	1	525 62
36	588	1.2	1	555 33
37	490	1.2	1	443 47
38	468	1.2	1	445 23
39	503	1.2	1	462 41
40	446	1.2	1	397 49
41	384	1.2	1	356 28
42	330	1.2	1	298 32
43	347	1.2	1	318 29
44	301	1.2	1	284 17
45	428	1.2	1	414 14
46	150	1.2	1	136 14
47	209	1.2	1	194 15
48	251	1.2	1	231 20
49	227	1.2	1	216 11
50	228	1.2	1	215 13
51	226	1.2	1	211 15
52	213	1.2	1	197 16
53	197	1.2	1	183 14
54	173	1.2	1	156 17
55	207	1.2	1	189 18
56	127	1.2	1	118 9
57	171	1.2	1	160 11
58	108	1.2	1	93 15
59	107	1.2	1	102 5
60	133	1.2	1	120 13
61	96	1.2	1	86 10
62	108	1.2	1	98 10
63	143	1.2	1	125 18
64	108	1.2	1	98 10
65	60	1.2	1	53 7
66	105	1.2	1	87 18
67	74	1.2	1	68 6
68	95	1.2	1	84 11
69	98	1.2	1	79 19
70	116	1.2	1	104 12
71	227	1.2	1	163 64
72	554	1.2	1	519 35
73	144	1.2	1	133 11
74	43	1.2	1	35 8
75	38	1.2	1	26 12
76	27	1.2	1	18 9
77	23	1.2	1	16 7
78	25	1.2	1	17 8
79	16	1.2	1	10 6
80	15	1.2	1	10 5
81	24	1.2	1	18 6
82	21	1.2	1	17 4
83	17	1.2	1	12 5
84	18	1.2	1	11 7
85	21	1.2	1	14 7
86	20	1.2	1	15 5
87	15	1.2	1	8 7
88	16	1.2	1	9 7
89	17	1.2	1	8 9
90	10	1.2	1	6 4
91	16	1.2	1	12 4
92	17	1.2	1	10 7
93	18	1.2	1	12 6
94	9	1.2	1	6 3
95	15	1.2	1	9 6
96	11	1.2	1	5 6
97	11	1.2	1	8 3
98	10	1.2	1	6 4
99	8	1.2	1	7 1
100	11	1.2	1	9 2
101	16	1.2	1	10 6
102	7	1.2	1	5 2
103	12	1.2	1	9 3
104	12	1.2	1	11 1
105	11	1.2	1	3 8
106	6	1.2	1	5 1
107	5	1.2	1	4 1
108	13	1.2	1	9 4
109	5	1.2	1	2 3
110	13	1.2	1	7 6
111	6	1.2	1	3 3
112	10	1.2	1	6 4
113	5	1.2	1	3 2
114	11	1.2	1	7 4
115	11	1.2	1	9 2
116	6	1.2	1	2 4
117	7	1.2	1	6 1
118	8	1.2	1	6 2
119	9	1.2	1	7 2
120	9	1.2	1	5 4
121	12	1.2	1	6 6
122	5	1.2	1	5
123	6	1.2	1	6
124	3	1.2	1	2 1
125	7	1.2	1	4 3
126	2	1.2	1	2
127	1	1.2	1	0 1
128	5	1.2	1	3 2
129	3	1.2	1	2 1
130	4	1.2	1	3 1
132	1	1.2	1	1
134	2	1.2	1	0 2
135	1	1.2	1	1
136	1	1.2	1	0 1
137	1	1.2	1	0 1
139	1	1.2	1	1
141	4	1.2	1	1 3
142	2	1.2	1	0 2
144	2	1.2	1	0 2
146	2	1.2	1	0 2
147	3	1.2	1	2 1
148	1	1.2	1	0 1
149	6	1.2	1	0 6
150	53	1.2	1	6 47

RUN STATISTICS FOR INPUT FILE: SRR10065364_1.fastq.gz
=============================================
79818903 sequences processed in total