Bismark report for: SRR546474_val_1.fq.gz and SRR546474_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_03/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 76205076 Number of paired-end alignments with a unique best hit: 42511969 Mapping efficiency: 55.8% Sequence pairs with no alignments under any condition: 22606771 Sequence pairs did not map uniquely: 11086336 Sequence pairs which were discarded because genomic sequence could not be extracted: 12 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 21231724 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 21280233 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1223982381 Total methylated C's in CpG context: 15940837 Total methylated C's in CHG context: 1019580 Total methylated C's in CHH context: 3961972 Total methylated C's in Unknown context: 29232 Total unmethylated C's in CpG context: 135325921 Total unmethylated C's in CHG context: 210700145 Total unmethylated C's in CHH context: 857033926 Total unmethylated C's in Unknown context: 2867255 C methylated in CpG context: 10.5% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in Unknown context (CN or CHN): 1.0% Bismark completed in 0d 7h 50m 29s