##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR546474_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77213856
Total Bases	6.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90
%GC	18
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.87837444616158	38.0	38.0	38.0	35.0	38.0
2	36.811482035037855	38.0	37.0	38.0	35.0	38.0
3	36.80969859865566	38.0	37.0	38.0	35.0	38.0
4	36.77080188819996	38.0	37.0	38.0	35.0	38.0
5	36.79707431008238	38.0	38.0	38.0	35.0	38.0
6	36.8025280333105	38.0	38.0	38.0	35.0	38.0
7	36.743242391624634	38.0	37.0	38.0	35.0	38.0
8	36.696367164981375	38.0	37.0	38.0	35.0	38.0
9	36.68306606783114	38.0	37.0	38.0	35.0	38.0
10-11	36.67798867602208	38.0	37.0	38.0	35.0	38.0
12-13	36.63336425006413	38.0	37.0	38.0	35.0	38.0
14-15	36.57389441216354	38.0	37.0	38.0	35.0	38.0
16-17	36.5418286906951	38.0	37.0	38.0	35.0	38.0
18-19	36.458849697909145	38.0	37.0	38.0	35.0	38.0
20-21	36.4051787803474	38.0	37.0	38.0	34.5	38.0
22-23	36.343864798048685	38.0	37.0	38.0	34.0	38.0
24-25	36.279344895040595	38.0	37.0	38.0	34.0	38.0
26-27	36.22630077560173	38.0	37.0	38.0	34.0	38.0
28-29	36.13441148438436	38.0	37.0	38.0	33.0	38.0
30-31	36.06010307269203	38.0	36.5	38.0	33.0	38.0
32-33	35.96902491827374	38.0	36.0	38.0	33.0	38.0
34-35	35.885005969653946	38.0	36.0	38.0	32.5	38.0
36-37	35.86050152708343	38.0	36.0	38.0	32.5	38.0
38-39	35.829322550605426	38.0	36.0	38.0	32.5	38.0
40-41	35.81123136759288	38.0	36.0	38.0	32.5	38.0
42-43	35.734438926350215	38.0	36.0	38.0	32.0	38.0
44-45	35.63123922343679	38.0	36.0	38.0	32.0	38.0
46-47	35.30383948834261	37.0	36.0	38.0	32.0	38.0
48-49	35.26371773221635	37.0	36.0	38.0	32.0	38.0
50-51	35.13097785195445	37.0	35.0	38.0	31.5	38.0
52-53	35.01446724795094	37.0	35.0	38.0	31.0	38.0
54-55	34.99987157745366	37.0	35.0	38.0	31.0	38.0
56-57	35.582209260472624	38.0	36.0	38.0	32.0	38.0
58-59	35.501297351345855	38.0	36.0	38.0	32.0	38.0
60-61	35.46299081993781	38.0	36.0	38.0	32.0	38.0
62-63	35.36812142887929	38.0	36.0	38.0	32.0	38.0
64-65	35.31670059969548	38.0	36.0	38.0	32.0	38.0
66-67	35.24040614549803	38.0	36.0	38.0	31.5	38.0
68-69	35.139876928306755	38.0	36.0	38.0	31.0	38.0
70-71	35.07088986075245	38.0	36.0	38.0	31.0	38.0
72-73	34.95170783855167	38.0	35.5	38.0	30.0	38.0
74-75	34.87086949782692	37.5	35.0	38.0	30.0	38.0
76-77	34.79933683793748	37.5	35.0	38.0	30.0	38.0
78-79	34.64727124882871	37.0	35.0	38.0	30.0	38.0
80-81	34.542975913545874	37.0	35.0	38.0	30.0	38.0
82-83	34.34552315325374	37.0	35.0	38.0	29.0	38.0
84-85	34.19008685435941	37.0	35.0	38.0	29.0	38.0
86-87	33.9367107893174	37.0	35.0	38.0	28.0	38.0
88-89	33.693389305670735	37.0	35.0	38.0	28.0	38.0
90	33.48187555870801	37.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	669830.0
3	80620.0
4	33240.0
5	35824.0
6	35140.0
7	48211.0
8	43300.0
9	59133.0
10	53161.0
11	72846.0
12	70254.0
13	90175.0
14	83598.0
15	97442.0
16	88406.0
17	102460.0
18	99578.0
19	118918.0
20	121603.0
21	153627.0
22	176041.0
23	220178.0
24	262363.0
25	325383.0
26	386662.0
27	477252.0
28	582176.0
29	727236.0
30	902840.0
31	1166230.0
32	1545163.0
33	2148001.0
34	3173986.0
35	5287831.0
36	1.1333905E7
37	4.6339306E7
38	1937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.4212088425055742	48.95085171812159	32.003227765613154	18.624711673759688
2	0.43790528919016736	52.43882656099898	31.61530787115024	15.507960278660601
3	0.3707943866597614	49.79858815829914	32.602245427354426	17.22837202768667
4	0.3622716359637829	49.54115962493641	32.516233828041564	17.580334911058237
5	0.3512617201976513	49.96312787825503	32.64218662008228	17.043423781465037
6	0.34190107331012753	49.950848429340944	32.041188982320946	17.66606151502798
7	0.34422537332896397	50.08279945324629	32.50837695837898	17.064598215045773
8	0.3202910010320205	49.55308333978756	32.82968224710488	17.296943412075542
9	0.317947800768179	49.14742958027631	33.26002206097576	17.274600557979756
10-11	0.3170418012757469	49.37971970333978	33.03663354318454	17.26660495219993
12-13	0.3051912221018875	49.30884419432087	33.071578022331046	17.314386561246195
14-15	0.30349443654318337	49.27824831016395	33.063544363791934	17.354712889500934
16-17	0.3044257290758992	49.221136908953746	33.011477648014775	17.46295971395558
18-19	0.3052597136360686	49.18029496016382	32.919271130581386	17.595174195618725
20-21	0.29978386297301957	49.18515746465227	32.86656036603593	17.648498306338777
22-23	0.2987371193647033	49.223543283163245	32.79956825003664	17.678151347435406
24-25	0.29866243125705194	49.26008573525568	32.70973931633146	17.731512517155814
26-27	0.3019816929399578	49.33873523070432	32.62897944107394	17.730303635281786
28-29	0.29273746534933287	49.38637345308681	32.59486406156864	17.726025019995216
30-31	0.2940095287442058	49.35645767624192	32.580533092256786	17.768999702757093
32-33	0.2937992257498548	49.379642376647	32.57886532972893	17.747693067874216
34-35	0.29257295638251124	49.41841144579057	32.53298421738454	17.756031380442383
36-37	0.30000268774072586	49.40226633555873	32.5096171435506	17.788113833149943
38-39	0.30697625385948063	49.41302582639447	32.492423060757964	17.787574858988087
40-41	0.30846440723636875	49.42210995692588	32.47934375878702	17.79008187705074
42-43	0.3100245680240026	49.39178029409282	32.48357082996972	17.814624307913462
44-45	0.31093894728609794	49.40884145422492	32.47510242254054	17.805117175948443
46-47	0.32246289721760707	49.41055536731595	32.44552606612363	17.821455669342814
48-49	0.3322381687158315	49.41067904116656	32.4245910990046	17.832491691113002
50-51	0.3323532028484826	49.40873299833254	32.4403961336742	17.818517665144785
52-53	0.33279640634731106	49.387126955169606	32.46741020386426	17.81266643461883
54-55	0.33849226212815964	49.368737382877484	32.46035200505086	17.832418349943495
56-57	0.34590485362976797	49.380091094700695	32.44337144365906	17.83063260801047
58-59	0.3531895093985755	49.38860914045854	32.43857771660028	17.8196236335426
60-61	0.36304184528299777	49.37365120750169	32.432825574439384	17.83048137277593
62-63	0.3708453790938599	49.36407338259761	32.44234847834766	17.822732759960864
64-65	0.3835181732110098	49.36499812734924	32.43555348328326	17.8159302161565
66-67	0.393228099725358	49.360308386689	32.43584705996727	17.810616453618373
68-69	0.4057150062989596	49.36848744833083	32.42730649572603	17.798491049644177
70-71	0.41839546476795064	49.357755643980454	32.427682923247	17.796165968004594
72-73	0.43141891089791023	49.33022759736269	32.44283127056883	17.79552222117056
74-75	0.4459562007149323	49.33382376646732	32.44401419285661	17.776205839961133
76-77	0.45962443945980136	49.330915044817246	32.43826629691673	17.771194218806226
78-79	0.4744358676346961	49.32322246739992	32.428926478400896	17.773415186564478
80-81	0.4921644518902429	49.32668872271663	32.42370088805727	17.75744593733586
82-83	0.5068078334070825	49.320346334419526	32.43734829233415	17.73549753983924
84-85	0.5228245090090089	49.294091852415136	32.44297463973549	17.740108998840366
86-87	0.5404110898487807	49.28620746126367	32.44831149896658	17.72506994992097
88-89	0.5562210153372206	49.28672495418252	32.44667923482773	17.710374795652527
90	0.5678178249570868	49.26768335011826	32.45247689417965	17.712021930745003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	393366.0
1	227817.5
2	59605.5
3	68175.0
4	112755.0
5	216351.5
6	414718.5
7	750176.5
8	1260271.0
9	1960284.0
10	2357543.0
11	2820213.0
12	3767022.0
13	4703421.5
14	5511384.5
15	6078684.5
16	6348068.0
17	6299876.0
18	5956814.0
19	5403543.5
20	5087357.0
21	4737392.0
22	4041367.0
23	3365758.0
24	2750037.0
25	2215438.0
26	1765120.5
27	1397513.0
28	1096643.0
29	857675.0
30	753860.0
31	669908.5
32	519222.5
33	398931.0
34	302748.5
35	229706.5
36	176607.5
37	135276.5
38	102605.5
39	76581.5
40	64580.0
41	55298.0
42	39256.0
43	27703.0
44	20167.5
45	15549.0
46	12073.0
47	9351.0
48	7828.0
49	9251.0
50	11075.0
51	7973.5
52	3993.0
53	2704.5
54	1937.5
55	1387.5
56	1030.0
57	785.0
58	611.0
59	446.5
60	376.0
61	329.5
62	245.0
63	182.5
64	133.5
65	107.5
66	97.5
67	73.0
68	69.5
69	77.5
70	73.0
71	63.5
72	50.0
73	53.0
74	55.0
75	43.5
76	43.0
77	43.5
78	43.0
79	40.5
80	33.0
81	25.5
82	29.5
83	33.0
84	21.0
85	21.5
86	24.5
87	23.0
88	20.0
89	18.5
90	20.0
91	16.5
92	13.0
93	11.5
94	11.5
95	15.0
96	17.5
97	14.5
98	11.5
99	21.5
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6228143819161162
2	0.6676729109345348
3	0.6593130124209831
4	0.6738427880094474
5	0.668719355241111
6	0.6450888814567168
7	0.5774054335532731
8	0.6566813604024646
9	0.7510219409324668
10-11	0.6081285462547034
12-13	0.6004291509544608
14-15	0.5710756110923926
16-17	0.6229691468847249
18-19	0.6460660376811126
20-21	0.6385926380881691
22-23	0.6463658543357814
24-25	0.592900061874905
26-27	0.6500400653478567
28-29	0.5874716320345406
30-31	0.5654198904403894
32-33	0.5786597421063908
34-35	0.5833648561729645
36-37	0.6171735549640209
38-39	0.6253275318875411
40-41	0.6049121546267551
42-43	0.6838125012173981
44-45	0.6401616569958636
46-47	0.6936280970089099
48-49	0.6408124469266242
50-51	0.634676242564547
52-53	0.6416976507428925
54-55	0.6349132466587344
56-57	0.6207635841940079
58-59	0.5968727944373092
60-61	0.6296790824693433
62-63	0.6307158134933709
64-65	0.641162772650546
66-67	0.6272766639189733
68-69	0.5992311794401253
70-71	0.5787704735274456
72-73	0.6396215984861577
74-75	0.5921411307317692
76-77	0.6305416219596649
78-79	0.6380111362395889
80-81	0.6464545689830592
82-83	0.6036837481604338
84-85	0.5787724161839554
86-87	0.5973895410688983
88-89	0.5831926072957683
90	0.5809397732966477
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
90	7.7213856E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.59872137155574
#Duplication Level	Percentage of total
1	83.57242238295427
2	7.2679201984621145
3	1.7885633061154709
4	0.9929709245811182
5	0.72254425449665
6	0.5863930045604678
7	0.4879836380427444
8	0.33951596638645193
9	0.3057648326205772
>10	2.74522935697453
>50	0.29597411552125297
>100	0.3507531422156035
>500	0.06561169035165408
>1k	0.03172301796454784
>5k	0.0
>10k+	0.44663016875258527
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	323705	0.41923175032211835	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.295104339822117E-6	0.0	0.0	0.0	0.24368424237224987	4.7918860573418324E-5
2	2.590208679644234E-6	0.0	0.0	0.0	0.3022501557233458	7.123073869021642E-5
3	5.180417359288468E-6	0.0	0.0	0.0	0.34834291917761495	8.806709510790394E-5
4	5.180417359288468E-6	0.0	0.0	0.0	0.39480090205571394	1.074936602052357E-4
5	6.4755216991105845E-6	0.0	0.0	0.0	0.4426511220991217	1.2562512096274533E-4
6	6.4755216991105845E-6	0.0	0.0	0.0	0.4893512894887674	1.4116637304061074E-4
7	7.770626038932701E-6	0.0	0.0	0.0	0.5350801286235465	1.6318314681758672E-4
8	7.770626038932701E-6	0.0	0.0	0.0	0.5799554939983829	1.8131460757509637E-4
9	7.770626038932701E-6	0.0	0.0	0.0	0.6249461236594634	2.0980690305118295E-4
10-11	7.770626038932701E-6	0.0	0.0	0.0	0.691908845997796	2.4218451154673585E-4
12-13	1.2303491228310111E-5	0.0	0.0	0.0	0.7803243241731121	2.7197191136264456E-4
14-15	2.3311878116798104E-5	0.0	0.0	0.0	0.8661308146558566	2.9657889381926473E-4
16-17	3.43202650052861E-5	0.0	0.0	0.0	0.9513447949031324	3.1212014589713014E-4
18-19	4.338599538404092E-5	0.0	0.0	0.0	1.0358231558853892	3.4190754571303885E-4
20-21	7.576360387959384E-4	0.0	0.0	0.0	1.1210054060763395	3.710473933590365E-4
22-23	8.062024515392678E-4	0.0	0.0	0.0	1.2069737846015616	3.898264062864572E-4
24-25	8.41170268714465E-4	0.0	0.0	2.590208679644234E-6	1.2926611254850424	4.027774496846784E-4
26-27	9.065730378754818E-4	0.0	0.0	2.590208679644234E-6	1.3779515168883678	4.202613582722769E-4
28-29	0.0010684610803532463	0.0	0.0	2.590208679644234E-6	1.4625406092916795	4.4163057987934185E-4
30-31	0.0014323853998432614	0.0	0.0	3.2377608495552923E-6	1.5468085158187153	4.5004875808818564E-4
32-33	0.0021006592391914734	0.0	0.0	3.885313019466351E-6	1.6310142573374395	4.597620406368515E-4
34-35	0.0033070489317357753	0.0	0.0	3.885313019466351E-6	1.7147220312375024	4.6818021884569526E-4
36-37	0.005286615915153881	0.0	0.0	3.885313019466351E-6	1.7984064932594481	4.7595084488462797E-4
38-39	0.00817987901031649	0.0	0.0	3.885313019466351E-6	1.8824425761096557	4.8307391875364964E-4
40-41	0.012166210168288966	0.0	0.0	3.885313019466351E-6	1.9653998370447916	4.927872013023155E-4
42-43	0.017231363241333265	0.0	0.0	4.532865189377409E-6	2.0469091713280063	4.953774099819597E-4
44-45	0.023605866801937724	0.0	0.0	7.123073869021643E-6	2.1287850201393903	4.999102751713371E-4
46-47	0.0318492059249055	0.0	0.0	7.770626038932701E-6	2.2098119280560216	5.018529316810703E-4
48-49	0.04168876114670403	0.0	0.0	7.770626038932701E-6	2.2902741704804903	5.050906925306257E-4
50-51	0.05313683595856163	0.0	0.0	7.770626038932701E-6	2.3716015426039596	5.076809012102699E-4
52-53	0.06640647502437905	0.0	0.0	9.065730378754818E-6	2.452683492455033	5.109186620598251E-4
54-55	0.08136233994064485	0.0	0.0	9.713282548665878E-6	2.534117063134368	5.167466315890246E-4
56-57	0.09820387677569166	0.0	0.0	1.0360834718576935E-5	2.615361548580089	5.199843924385799E-4
58-59	0.11738825736147668	0.0	0.0	1.0360834718576935E-5	2.696549049434858	5.20631944608491E-4
60-61	0.13844017840528516	0.0	0.0	1.0360834718576935E-5	2.7772353449101157	5.225746011182242E-4
62-63	0.161208112699358	0.0	0.0	1.0360834718576935E-5	2.8575337566355965	5.284025706474237E-4
64-65	0.18585848115136228	0.0	0.0	1.0360834718576935E-5	2.9380931318855517	5.355256445164453E-4
66-67	0.21194447276405934	0.0	0.0	1.1655939058399052E-5	3.018499684823408	5.394109575359116E-4
68-69	0.2398792258218525	0.0	0.0	1.1655939058399052E-5	3.099144536959791	5.43296270555378E-4
70-71	0.26934802997016494	0.0	0.0	1.2951043398221169E-5	3.179508999006603	5.491242400845777E-4
72-73	0.3007439493761327	0.0	0.0	1.2951043398221169E-5	3.2595704066378968	5.530095531040439E-4
74-75	0.3333708654571014	0.0	0.0	1.2951043398221169E-5	3.339726356885997	5.588375226332435E-4
76-77	0.36711480903116667	0.0	0.0	1.2951043398221169E-5	3.4204961865911736	5.640179399925319E-4
78-79	0.402059314328247	0.0	0.0	1.2951043398221169E-5	3.5009526010461127	5.64665492162443E-4
>>END_MODULE
