##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR546474_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77213856
Total Bases	6.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.077815411783085	38.0	37.0	38.0	35.0	38.0
2	36.97401949463578	38.0	37.0	38.0	35.0	38.0
3	36.9510896334461	38.0	37.0	38.0	35.0	38.0
4	36.876058592385284	38.0	37.0	38.0	35.0	38.0
5	36.941842795676465	38.0	37.0	38.0	35.0	38.0
6	36.981412999242	38.0	37.0	38.0	35.0	38.0
7	36.93870782725836	38.0	37.0	38.0	35.0	38.0
8	36.887558859902036	38.0	37.0	38.0	35.0	38.0
9	36.884896190134576	38.0	37.0	38.0	35.0	38.0
10-11	36.82172776088271	38.0	37.0	38.0	35.0	38.0
12-13	36.750179527363585	38.0	37.0	38.0	35.0	38.0
14-15	36.72026779753105	38.0	36.5	38.0	35.0	38.0
16-17	36.63057247134504	38.0	36.0	38.0	34.0	38.0
18-19	36.58639575648184	38.0	36.0	38.0	34.0	38.0
20-21	36.51527715440089	38.0	36.0	38.0	34.0	38.0
22-23	36.393448120244116	38.0	36.0	38.0	33.5	38.0
24-25	36.35399791457119	38.0	36.0	38.0	33.0	38.0
26-27	36.13037510391917	38.0	36.0	38.0	33.0	38.0
28-29	35.995043577411806	37.0	36.0	38.0	32.5	38.0
30-31	35.86100710344009	37.0	36.0	38.0	32.0	38.0
32-33	35.781621740274176	37.0	36.0	38.0	32.0	38.0
34-35	35.80928541504261	37.0	36.0	38.0	32.0	38.0
36-37	35.87011637522674	37.5	36.0	38.0	32.5	38.0
38-39	35.90815887371303	38.0	36.0	38.0	33.0	38.0
40-41	35.885440211663564	37.5	36.0	38.0	33.0	38.0
42-43	35.85446557027278	38.0	36.0	38.0	32.5	38.0
44-45	35.767470471880074	37.5	36.0	38.0	32.0	38.0
46-47	35.66967944199031	37.5	36.0	38.0	32.0	38.0
48-49	35.553893015004974	37.0	36.0	38.0	32.0	38.0
50-51	35.456590394863845	37.0	36.0	38.0	32.0	38.0
52-53	35.34667355558567	37.0	35.0	38.0	31.5	38.0
54-55	35.23989731195396	37.0	35.0	38.0	31.0	38.0
56-57	35.07931105655441	37.0	35.0	38.0	30.5	38.0
58-59	34.96417637787705	37.0	35.0	38.0	30.0	38.0
60-61	34.81320313804817	37.0	35.0	38.0	30.0	38.0
62-63	34.71157246699349	37.0	35.0	38.0	30.0	38.0
64-65	34.56368404914268	37.0	35.0	38.0	30.0	38.0
66-67	34.40476046812116	37.0	34.5	38.0	29.0	38.0
68-69	34.246801500238504	36.5	34.0	38.0	29.0	38.0
70-71	33.86849346702748	36.0	33.5	38.0	28.0	38.0
72-73	33.43223341287346	36.0	33.0	37.0	27.5	38.0
74-75	33.256047593323146	36.0	33.0	37.0	27.0	38.0
76-77	33.106225364525244	36.0	33.0	37.0	26.0	38.0
78-79	33.03076365594279	36.0	33.0	37.0	26.0	38.0
80-81	33.24648021075389	36.0	33.5	37.5	26.0	38.0
82-83	33.36776011419505	36.0	33.5	38.0	26.0	38.0
84-85	33.11627886450846	36.0	33.0	38.0	25.0	38.0
86-87	32.66715095150798	36.0	33.0	38.0	24.5	38.0
88-89	32.31846208405911	36.0	32.5	38.0	21.5	38.0
90	32.01205011701527	36.0	32.0	38.0	20.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15264.0
3	3382.0
4	5285.0
5	8404.0
6	12975.0
7	20053.0
8	33498.0
9	45565.0
10	59997.0
11	87051.0
12	83225.0
13	111808.0
14	97232.0
15	122690.0
16	123179.0
17	152088.0
18	149045.0
19	171691.0
20	185791.0
21	220101.0
22	247081.0
23	301857.0
24	356487.0
25	450633.0
26	554843.0
27	703224.0
28	876192.0
29	1115722.0
30	1405064.0
31	1805940.0
32	2355505.0
33	3216407.0
34	4752370.0
35	7788760.0
36	1.6261098E7
37	3.3313846E7
38	503.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.17572267190322	34.79549281451647	48.324268474105466	0.704516039474837
2	17.96155635696811	34.89150545579863	46.732235167537226	0.41470301969603945
3	16.904890789367332	33.455862854933926	49.24846244723723	0.39078390846151245
4	17.457770539164535	33.052365794276575	49.12094029682932	0.368923369729576
5	17.495410845766575	33.19823144049776	48.97238923301232	0.3339684807233505
6	17.45345679925634	33.10065488764089	49.11228627152101	0.33360204158175966
7	17.63474669007022	33.13031233971124	48.928924611044685	0.3060163591738571
8	16.967058347662366	33.076326352617336	49.65129833692025	0.3053169628000446
9	17.028088844572146	32.64816226766346	50.020043034763084	0.30370585300130587
10-11	17.08658417473672	32.893236804544514	49.72655490745081	0.2936241132679606
12-13	16.998629753706382	32.94061236884737	49.76823266021062	0.2925252172356215
14-15	17.06189624825886	32.8418872125749	49.80673287447269	0.2894836646935493
16-17	17.165458619240567	32.69099395839006	49.85327244892419	0.2902749734451806
18-19	17.224108066821582	32.57513068638872	49.91116102270556	0.2896002240841333
20-21	17.286895372768328	32.50737859795527	49.91549120406576	0.29023482521064614
22-23	17.35808068617454	32.48065321327401	49.87079367549161	0.2904724250598364
24-25	17.44012024567899	32.46412443298016	49.803408771968805	0.29234654937205085
26-27	17.509737652635476	32.496217508061065	49.703594115622565	0.29045072368089336
28-29	17.54555445754032	32.46788791255371	49.69837775233856	0.28817987756742175
30-31	17.547024616793884	32.435058975288264	49.72883988237183	0.2890765255460214
32-33	17.588319409319126	32.434086419355104	49.68672880684677	0.2908653644789956
34-35	17.636503060643836	32.41967236164037	49.65176385587809	0.29206072183770987
36-37	17.65694831004573	32.38927273900004	49.66213620176705	0.29164274918718386
38-39	17.69520297013169	32.37954458678397	49.63123792944395	0.2940145136403909
40-41	17.69474097395048	32.373365924422565	49.63595473316523	0.2959383684617295
42-43	17.70129701733586	32.34527965559943	49.65685714594935	0.2965661811153626
44-45	17.729874116103282	32.33550600011612	49.637282355810754	0.2973375279698454
46-47	17.761173295370767	32.32248317952573	49.616393988831284	0.29994953627222143
48-49	17.76979513777625	32.30030457510534	49.627618255543844	0.3022820315745678
50-51	17.788953575707218	32.292242092688646	49.61507391472274	0.3037304168813919
52-53	17.789560705465288	32.27886502622434	49.62532453811641	0.306249730193965
54-55	17.808888196906533	32.24320624212319	49.63756529036946	0.31034027060081765
56-57	17.826535823136755	32.238779253806115	49.62161566937433	0.313069253682803
58-59	17.847161358240715	32.23161860456036	49.6035906680927	0.3176293691062253
60-61	17.843829595438525	32.19736631181206	49.636670023432764	0.3221340693166505
62-63	17.85008607846584	32.19442404185921	49.62800516150889	0.32748471816606584
64-65	17.872347363524472	32.1833576364107	49.612474605885204	0.33182039417963216
66-67	17.88380768199131	32.1634206256364	49.61374379691594	0.3390278954563421
68-69	17.89757233340826	32.15836862145335	49.59872406582521	0.34533497931318147
70-71	17.911721026313185	32.14213742370599	49.59320657663441	0.35293497334641466
72-73	17.906460999742517	32.114337310737206	49.61808753925771	0.36111415026256677
74-75	17.93543014757742	32.11391617813576	49.58049275754431	0.37016091674250473
76-77	17.74738285402282	32.147920139520956	49.731701037090836	0.3729959693653876
78-79	17.778156861400994	32.132454999578236	49.709214744132154	0.3801733948886159
80-81	17.781323691080242	32.109573264869596	49.71736963510761	0.3917334089425562
82-83	17.77229356459842	32.0939418456755	49.733068747177086	0.4006958425489897
84-85	17.74166699864679	32.08294847178268	49.762736323985585	0.41264820558494786
86-87	17.763600513001137	32.065647107413994	49.74290208915264	0.4278502904322256
88-89	17.743603319002972	32.04603478687022	49.775053092448054	0.43530880167875596
90	17.731680333988635	32.033692525396084	49.79156208173327	0.4430650588820151
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26980.0
1	25518.5
2	30942.0
3	54853.5
4	107731.5
5	215918.0
6	419779.0
7	764290.5
8	1292368.0
9	2018607.5
10	2429755.0
11	2912655.5
12	3893524.0
13	4846965.0
14	5652269.0
15	6205098.0
16	6450111.0
17	6369732.0
18	5994307.5
19	5414392.0
20	5087507.0
21	4728081.0
22	4015425.0
23	3328497.5
24	2701532.0
25	2160187.0
26	1709312.0
27	1341819.5
28	1046357.0
29	813368.0
30	711147.0
31	633078.5
32	491417.0
33	377207.0
34	286560.0
35	217598.0
36	167218.5
37	128332.0
38	97359.5
39	72899.0
40	61972.0
41	53453.0
42	38695.0
43	27640.0
44	19931.5
45	14941.5
46	11242.5
47	8454.0
48	6624.0
49	9213.5
50	12446.0
51	9020.5
52	4547.5
53	2993.5
54	2123.0
55	1523.0
56	1137.0
57	824.0
58	611.0
59	498.0
60	435.0
61	379.5
62	269.0
63	193.0
64	132.5
65	95.5
66	81.0
67	54.5
68	41.0
69	36.0
70	35.0
71	28.5
72	22.5
73	19.0
74	14.0
75	12.5
76	10.0
77	7.0
78	6.0
79	6.0
80	6.0
81	7.5
82	5.5
83	3.0
84	2.5
85	2.0
86	4.5
87	4.5
88	3.5
89	4.0
90	4.0
91	2.5
92	5.0
93	8.0
94	4.5
95	5.5
96	9.5
97	14.0
98	21.0
99	40.0
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02205951222019012
2	0.0059937428846967575
3	1.074936602052357E-4
4	0.2141260760244897
5	3.6521942382983696E-4
6	0.0
7	0.0021291515346675604
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.010459910200573326
24-25	0.0042278681173493005
26-27	0.004549701545795096
28-29	0.032490282573117445
30-31	0.07734168851766708
32-33	0.12372131758320683
34-35	0.11345114016841744
36-37	0.1066272354018947
38-39	0.12859803297480701
40-41	0.18770918525296806
42-43	0.19921553976011766
44-45	0.26842721078455145
46-47	0.26688085620280383
48-49	0.22990756995739212
50-51	0.3214908733479131
52-53	0.2472755667065766
54-55	0.3235002277311471
56-57	0.27668673871176697
58-59	0.2939336432051781
60-61	0.29535243000945327
62-63	0.20725749015824307
64-65	0.27138587664887504
66-67	0.27160992969966424
68-69	0.27994133591773995
70-71	0.22648396163507234
72-73	0.22994642308758675
74-75	0.2767579694504572
76-77	0.2639092392950819
78-79	0.22570107106164985
80-81	0.2411691497443153
82-83	0.20191518475647685
84-85	0.2768460365455651
86-87	0.21255835222113503
88-89	0.24997456415076588
90	0.2062557269513907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
90	7.7213856E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.87990528722024
#Duplication Level	Percentage of total
1	83.65106260117828
2	7.642303094174256
3	1.832351453107634
4	1.0002987808357515
5	0.6906566471764702
6	0.5909468485381988
7	0.4790794565030699
8	0.3450017314744275
9	0.29705554390612826
>10	2.7865318238652788
>50	0.31884243391056527
>100	0.24671146293527643
>500	0.06542183175987913
>1k	0.030330406914198017
>5k	0.0
>10k+	0.0234058837206129
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.590208679644234E-6	0.0	0.0	0.0	0.008396161435066784	8.405227165445538E-4
2	3.885313019466351E-6	0.0	0.0	0.0	0.011387852460055874	0.0012562512096274533
3	7.770626038932701E-6	0.0	0.0	0.0	0.014312198059374214	0.0016810454330891077
4	7.770626038932701E-6	0.0	0.0	0.0	0.01710573812037052	0.0021006592391914734
5	9.065730378754818E-6	0.0	0.0	0.0	0.01992518026816327	0.002670505148713205
6	9.065730378754818E-6	0.0	0.0	0.0	0.02265396511216847	0.0033387789880614176
7	1.1655939058399052E-5	0.0	0.0	0.0	0.025527801642233745	0.004118431800634332
8	1.1655939058399052E-5	0.0	0.0	0.0	0.028291554303414145	0.004988741916994794
9	1.1655939058399052E-5	0.0	0.0	0.0	0.031118767077245826	0.00605720299734804
10-11	1.4246147738043286E-5	0.0	0.0	0.0	0.03523072335618105	0.008003097267930772
12-13	2.007411726724281E-5	0.0	0.0	0.0	0.04078154055665864	0.011431238455439915
14-15	3.1082504155730806E-5	0.0	0.0	0.0	0.046138739658332824	0.015499161186821184
16-17	4.338599538404092E-5	0.0	0.0	0.0	0.051277713678746985	0.019905753703065937
18-19	5.309927793270679E-5	0.0	0.0	0.0	0.05615119130949761	0.024414659462156638
20-21	8.502359990932198E-4	0.0	0.0	0.0	0.06123382829112951	0.0291029371723127
22-23	8.981548596666381E-4	0.0	0.0	0.0	0.06602506679630143	0.033744591126235166
24-25	9.279422594825467E-4	0.0	0.0	0.0	0.07069586059786989	0.03850021426206198
26-27	9.926974764736525E-4	0.0	0.0	0.0	0.07545536904671618	0.04315999967674196
28-29	0.0011610610406505278	0.0	0.0	0.0	0.08023559916499962	0.04790461442568028
30-31	0.0015431168208980524	0.0	0.0	0.0	0.08495625448365123	0.052790395547659213
32-33	0.0022437682687418176	0.0	0.0	0.0	0.08951437420765516	0.05776424376474606
34-35	0.003488363539310872	0.0	0.0	0.0	0.09428230083471029	0.06280155209448418
36-37	0.00554304657443866	0.0	0.0	0.0	0.09893755338420089	0.06780583526355684
38-39	0.008550278851505616	0.0	0.0	0.0	0.10365756115068259	0.07284702890631442
40-41	0.012719867273562922	0.0	0.0	0.0	0.10860421217663316	0.07805011059154979
42-43	0.01802202444079467	0.0	0.0	0.0	0.11346797652483512	0.08316447762950732
44-45	0.024681450956159992	0.0	0.0	0.0	0.11831296186010967	0.08841871593616565
46-47	0.03328936195078769	0.0	0.0	0.0	0.12316701292576296	0.09381023530284513
48-49	0.043543350561329304	0.0	0.0	0.0	0.12800876050018795	0.09924578821707855
50-51	0.05550817200477593	0.0	0.0	0.0	0.1328375570312147	0.10467292295310313
52-53	0.06930038567171157	0.0	0.0	0.0	0.13774211716612106	0.11021596952754179
54-55	0.08485653144948492	0.0	0.0	0.0	0.14263825912281858	0.11578815594962645
56-57	0.10239548197152594	0.0	0.0	0.0	0.14758231994008952	0.12152676327937825
58-59	0.1223543349525246	0.0	0.0	0.0	0.15249076538801531	0.12720061539213895
60-61	0.14434844440355368	0.0	0.0	0.0	0.15743935907047563	0.13302146184746944
62-63	0.1682683740078983	0.0	0.0	0.0	0.1622908199274493	0.1388908747155433
64-65	0.19410376293083975	0.0	0.0	0.0	0.16722516746217156	0.14476870576182596
66-67	0.22133786454078916	0.0	0.0	0.0	0.17212325207537882	0.15013432304170898
68-69	0.250665502316061	0.0	0.0	0.0	0.17722790583078768	0.15555498225603448
70-71	0.28127270317907704	0.0	0.0	0.0	0.18251711195462123	0.16103651137433156
72-73	0.3138024864345591	0.0	0.0	0.0	0.18763795451427784	0.1665471803402747
74-75	0.34765262856448975	0.0	0.0	0.0	0.1927944124432796	0.17206626748442663
76-77	0.3827745631561258	0.0	0.0	0.0	0.19817686607958035	0.1773412274605221
78-79	0.4190251811799167	0.0	0.0	0.0	0.20339613656906347	0.18268936083181755
>>END_MODULE
