##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR546473_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78826347
Total Bases	7 Gbp
Sequences flagged as poor quality	0
Sequence length	90
%GC	18
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.20537043280719	38.0	38.0	38.0	36.0	38.0
2	37.17862709025448	38.0	38.0	38.0	36.0	38.0
3	37.15659822470271	38.0	38.0	38.0	36.0	38.0
4	37.15204186742283	38.0	38.0	38.0	36.0	38.0
5	37.08181230318843	38.0	38.0	38.0	36.0	38.0
6	37.16527325565398	38.0	38.0	38.0	36.0	38.0
7	37.12514528930283	38.0	38.0	38.0	36.0	38.0
8	37.00310894782426	38.0	38.0	38.0	36.0	38.0
9	37.09070892248755	38.0	38.0	38.0	36.0	38.0
10-11	37.081165070353954	38.0	38.0	38.0	36.0	38.0
12-13	37.044268923181235	38.0	38.0	38.0	36.0	38.0
14-15	37.012241205342164	38.0	38.0	38.0	35.5	38.0
16-17	36.98041783542246	38.0	38.0	38.0	35.5	38.0
18-19	36.92541413215558	38.0	38.0	38.0	35.0	38.0
20-21	36.87312870149875	38.0	38.0	38.0	35.0	38.0
22-23	36.78661845131552	38.0	37.0	38.0	35.0	38.0
24-25	36.733102182192965	38.0	37.0	38.0	35.0	38.0
26-27	36.628296577031534	38.0	37.0	38.0	35.0	38.0
28-29	36.64442265731279	38.0	37.0	38.0	35.0	38.0
30-31	36.6221841473892	38.0	37.0	38.0	35.0	38.0
32-33	36.5796557399774	38.0	37.0	38.0	35.0	38.0
34-35	36.522513031080834	38.0	37.0	38.0	35.0	38.0
36-37	36.438632935761945	38.0	37.0	38.0	34.5	38.0
38-39	36.46105832482634	38.0	37.0	38.0	35.0	38.0
40-41	36.417432099193945	38.0	37.0	38.0	34.5	38.0
42-43	36.35172921307644	38.0	37.0	38.0	34.0	38.0
44-45	36.26488440470291	38.0	37.0	38.0	34.0	38.0
46-47	35.85030800044559	38.0	36.0	38.0	33.0	38.0
48-49	35.80101964511942	38.0	36.0	38.0	33.0	38.0
50-51	35.670362937153484	37.5	36.0	38.0	32.5	38.0
52-53	35.52646840909677	37.0	36.0	38.0	32.0	38.0
54-55	35.51574561738856	37.0	36.0	38.0	32.0	38.0
56-57	36.026868897019924	38.0	36.5	38.0	33.5	38.0
58-59	35.96674594726558	38.0	36.0	38.0	33.0	38.0
60-61	35.92941852931482	38.0	36.0	38.0	33.0	38.0
62-63	35.8560220988041	38.0	36.0	38.0	33.0	38.0
64-65	35.79885540046655	38.0	36.0	38.0	33.0	38.0
66-67	35.7165994638823	38.0	36.0	38.0	33.0	38.0
68-69	35.62818341055434	38.0	36.0	38.0	32.0	38.0
70-71	35.566989905037715	38.0	36.0	38.0	32.0	38.0
72-73	35.48395707085094	38.0	36.0	38.0	32.0	38.0
74-75	35.41814902953704	38.0	36.0	38.0	32.0	38.0
76-77	35.35282198222379	38.0	36.0	38.0	32.0	38.0
78-79	35.236166030883055	37.5	36.0	38.0	32.0	38.0
80-81	35.1534459664863	37.5	36.0	38.0	32.0	38.0
82-83	34.998890681817336	37.0	35.0	38.0	31.0	38.0
84-85	34.87614408289147	37.0	35.0	38.0	31.0	38.0
86-87	34.6693008049707	37.0	35.0	38.0	30.5	38.0
88-89	34.470065116679834	37.0	35.0	38.0	30.0	38.0
90	34.296227985802766	37.0	35.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	304885.0
3	19026.0
4	16679.0
5	24437.0
6	27245.0
7	39972.0
8	37714.0
9	60902.0
10	49760.0
11	71815.0
12	68466.0
13	90030.0
14	78396.0
15	90340.0
16	76366.0
17	90247.0
18	81394.0
19	102399.0
20	102238.0
21	134445.0
22	152604.0
23	196561.0
24	232943.0
25	290466.0
26	338299.0
27	412781.0
28	481988.0
29	586708.0
30	707356.0
31	888549.0
32	1134813.0
33	1541618.0
34	2238217.0
35	3863005.0
36	9490307.0
37	5.4700966E7
38	2410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.3917176039873181	48.88883168172205	31.959259681771552	18.76019103251908
2	0.40375938535460426	52.37777813457151	31.45406067902882	15.764401801045064
3	0.344812020983339	49.67191284020488	32.42427094780765	17.559004191004128
4	0.3362263625306495	49.37490360176709	32.32668566629404	17.96218436940822
5	0.32191638560019725	49.8334961144041	32.4389148115991	17.405672688396603
6	0.30412611272816575	49.82353121279605	31.82210019250932	18.050242481966464
7	0.32271195063262936	50.00018158134342	32.24905483736254	17.428051630661415
8	0.28980281430019533	49.463149542474376	32.565553225617336	17.68149441760809
9	0.2948204831720452	49.04127146410108	32.99095764981596	17.672950402910914
10-11	0.29570826099632613	49.29187992476598	32.74149352954409	17.67091828469361
12-13	0.28648365895048017	49.2267752716434	32.76879736503023	17.71794370437589
14-15	0.28711543936292216	49.203234441555374	32.76685451519629	17.742795603885416
16-17	0.28929853113856757	49.147140973312794	32.694283530390564	17.86927696515808
18-19	0.2936599163113943	49.1003819456576	32.589607498685474	18.01635063934553
20-21	0.28908167162415577	49.093299944025986	32.53932540465038	18.07829297969948
22-23	0.2918435214210214	49.12873764660276	32.46912518998999	18.11029364198623
24-25	0.2938713829728485	49.16716166909447	32.36802875919345	18.170938188739232
26-27	0.2977576797597866	49.23445406301127	32.29156768876481	18.17622056846413
28-29	0.2943777546420631	49.27587312833309	32.24817361752823	18.181575499496617
30-31	0.2949209273546919	49.23991924116546	32.22717317913114	18.237986652348713
32-33	0.29571706611533244	49.26954105588365	32.217483524523004	18.217258353478012
34-35	0.29634871358581916	49.30120613570219	32.175292820102634	18.227152330609357
36-37	0.3008373108230096	49.29116831966995	32.151700227955324	18.256294141551717
38-39	0.3037166763259994	49.28636627014982	32.146404999388764	18.26351205413541
40-41	0.3090312018238731	49.292112245172284	32.12708164589765	18.2717749071062
42-43	0.3184220103481167	49.25658125490463	32.125249950158924	18.299746784588333
44-45	0.323370669471857	49.27815849688925	32.116447026208036	18.282023807430864
46-47	0.33157832916823693	49.280216245003864	32.104536963646396	18.283668462181506
48-49	0.34372179200610214	49.283815904992814	32.07389292722343	18.29856937577766
50-51	0.35950980193439547	49.28728010901901	32.07568144489755	18.27752864414905
52-53	0.37273046869013743	49.27074194512409	32.08870264278404	18.267824943401735
54-55	0.3952762284217478	49.24149052611628	32.064548311191615	18.298684934270355
56-57	0.4154950964446453	49.25893494794211	32.04064140972476	18.284928545888487
58-59	0.44128818560790267	49.25326644811521	32.02153058457967	18.283914781697213
60-61	0.4712011428333469	49.21725353033195	32.017659665452044	18.293885661382657
62-63	0.5008991476642485	49.206631781175	32.000776689990964	18.291692381169796
64-65	0.5341596299626519	49.19509016458059	31.988011268752942	18.282738936703822
66-67	0.5674621341487629	49.1697524323895	31.981145784520077	18.28163964894166
68-69	0.6039394221088505	49.16979652200422	31.963894877927384	18.26236917795955
70-71	0.6453540152773282	49.14913971858489	31.947159086058235	18.258347180079546
72-73	0.6851439599925785	49.11274565532158	31.92787244113292	18.274237943552922
74-75	0.7277306771556664	49.1007873137589	31.921324907885797	18.250157101199637
76-77	0.7731519827121212	49.07334663270105	31.901682868901197	18.251818515685628
78-79	0.8211605159791961	49.04972927170713	31.88079099142985	18.248319220883825
80-81	0.8696613963704658	49.03759601521961	31.862585606846398	18.230156981563518
82-83	0.9188531173960821	49.00542935841258	31.8481754462051	18.227542077986243
84-85	0.9695399322969698	48.955599234115475	31.837506787175556	18.237354046411998
86-87	1.0202564777206922	48.94473317237	31.815867440945738	18.21914290896357
88-89	1.0734221785531883	48.910245405598545	31.809171254869412	18.207161160978856
90	1.11148405570467	48.859636221871725	31.801847403512262	18.227032318911345
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26439.0
1	26761.0
2	29028.5
3	37388.0
4	61691.0
5	122229.5
6	255295.0
7	512009.5
8	948614.5
9	1604208.0
10	1989495.0
11	2472280.0
12	3489762.0
13	4542205.0
14	5485299.0
15	6182027.5
16	6556752.5
17	6585560.0
18	6289676.0
19	5755100.5
20	5441872.0
21	5082524.0
22	4362936.0
23	3661252.0
24	3015265.5
25	2450402.0
26	1973797.0
27	1579867.0
28	1255027.0
29	992549.0
30	877265.0
31	784807.5
32	617436.0
33	480850.5
34	367880.0
35	281497.0
36	220005.5
37	170457.5
38	129989.0
39	98520.5
40	84380.0
41	72460.5
42	52401.5
43	37996.5
44	27762.5
45	21158.5
46	16708.5
47	13594.0
48	11629.5
49	15481.0
50	19997.0
51	13931.5
52	6607.0
53	4701.5
54	3612.0
55	2926.0
56	2431.0
57	2045.5
58	1788.0
59	1586.5
60	1509.0
61	1397.0
62	1244.0
63	1173.0
64	1104.5
65	1040.0
66	1007.5
67	1023.5
68	1026.0
69	978.5
70	951.0
71	951.0
72	936.5
73	920.5
74	858.5
75	816.0
76	808.0
77	774.5
78	749.0
79	716.0
80	701.0
81	682.0
82	613.5
83	559.5
84	541.5
85	506.0
86	448.0
87	394.5
88	377.5
89	336.5
90	295.0
91	278.5
92	238.5
93	187.0
94	152.5
95	121.0
96	79.5
97	63.5
98	62.5
99	110.0
100	159.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15988182225417602
2	0.1274941739974326
3	0.14485512058550679
4	0.1641139097819667
5	0.3995694485246158
6	0.14370449007360445
7	0.09357658042938359
8	0.42679384850854496
9	0.1076683155189216
10-11	0.09717880241234571
12-13	0.09968050403249057
14-15	0.057268923041683004
16-17	0.03396897232850331
18-19	0.040126177609118435
20-21	0.0368721894470132
22-23	0.10327638295860647
24-25	0.0941411124886962
26-27	0.0398369342169313
28-29	0.10805143615243264
30-31	0.07609130992712373
32-33	0.04738707478097393
34-35	0.10246764321071482
36-37	0.09174470561219841
38-39	0.1118426812294118
40-41	0.07738465921806575
42-43	0.14101122813670408
44-45	0.20391659149192842
46-47	0.3541265206670049
48-49	0.1968428144970361
50-51	0.20305837590063636
52-53	0.1655195311790866
54-55	0.1892958454614166
56-57	0.1427720607172117
58-59	0.10920079805296572
60-61	0.10853921722390611
62-63	0.08433791813288012
64-65	0.12908501265446184
66-67	0.17217847225623686
68-69	0.15877813036293564
70-71	0.21996769684126047
72-73	0.15534082785797496
74-75	0.10468327296709563
76-77	0.0684168454488954
78-79	0.08363130160021243
80-81	0.07057538769366034
82-83	0.05630921854085157
84-85	0.056448765791468174
86-87	0.04848252069831423
88-89	0.05046345735138532
90	0.06075252986162101
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
90	7.8826347E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.40741911315756
#Duplication Level	Percentage of total
1	83.11724250676588
2	7.686003420838435
3	1.8660151420809463
4	1.0091154337629986
5	0.7485659301207487
6	0.5667918637530773
7	0.4779739331725417
8	0.3649772669210419
9	0.3070183938257832
>10	3.066596532017244
>50	0.3862329205531187
>100	0.27981013893727014
>500	0.05873808395727169
>1k	0.03770080272725431
>5k	0.0064339340529809075
>10k+	0.0207836965134448
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.537222738483619E-6	0.0	0.0	0.0	0.23047370189563648	7.611668215450857E-5
2	3.8058341077254285E-6	0.0	0.0	0.0	0.28522316275800524	1.1163780049327924E-4
3	7.611668215450857E-6	0.0	0.0	0.0	0.328404156544258	1.3701002787811542E-4
4	8.880279584692667E-6	0.0	0.0	0.0	0.3719467045707446	1.7253114621688608E-4
5	1.2686113692418095E-5	0.0	0.0	0.0	0.41717904294106134	1.9409753949399685E-4
6	1.7760559169385334E-5	0.0	0.0	0.0	0.46198766511405126	2.2454421235580029E-4
7	2.410361601559438E-5	0.0	0.0	0.0	0.5057547573528937	2.511850511098783E-4
8	2.6640838754077998E-5	0.0	0.0	0.0	0.5479906356690613	2.854375580794071E-4
9	2.790945012331981E-5	0.0	0.0	0.0	0.5910663347116669	3.184214536796942E-4
10-11	3.9961258131117E-5	0.0	0.0	1.2686113692418096E-6	0.6542495239567552	3.6662868571088296E-4
12-13	5.4550288877397803E-5	0.0	0.0	1.2686113692418096E-6	0.7378878029194985	4.4528259060387513E-4
14-15	1.06563355016312E-4	0.0	0.0	1.2686113692418096E-6	0.819827411258827	5.156905215967956E-4
16-17	1.604793382090889E-4	0.0	0.0	3.8058341077254285E-6	0.90105659723138	5.880013696435787E-4
18-19	1.769712860092324E-4	0.0	0.0	5.708751161588143E-6	0.9808547895794283	6.672895802211918E-4
20-21	0.002322827417081753	0.0	0.0	6.3430568462090476E-6	1.0604658617505134	7.351602884756286E-4
22-23	0.0024465170255828297	0.0	0.0	6.3430568462090476E-6	1.139681127174395	7.947850228299936E-4
24-25	0.00251692495657575	0.0	0.0	6.3430568462090476E-6	1.2188729232879458	8.334776695918688E-4
26-27	0.002688187491423394	0.0	0.0	6.3430568462090476E-6	1.2982676972205751	8.778790675153321E-4
28-29	0.0031924605106970136	0.0	0.0	6.3430568462090476E-6	1.375859774397512	9.114972688002401E-4
30-31	0.004216229885675154	0.0	0.0	6.3430568462090476E-6	1.4536535607821581	9.406753302928018E-4
32-33	0.006182577507999958	0.0	0.0	6.977362530829952E-6	1.5309766923488157	9.616074178852916E-4
34-35	0.009926883964317158	0.0	0.0	7.611668215450857E-6	1.6078171172894768	9.793679770546768E-4
36-37	0.015831635582453162	0.0	0.0	7.611668215450857E-6	1.683932530832616	9.888825623239905E-4
38-39	0.02455841319146757	0.0	0.0	7.611668215450857E-6	1.7601944689889029	0.001004105898754892
40-41	0.036484628673709815	0.0	0.0	8.245973900071762E-6	1.8369885896145866	0.0010167920124473104
42-43	0.05166483231805731	0.0	0.0	8.880279584692667E-6	1.9131750453944034	0.00103074673750897
44-45	0.07136509827101337	0.0	0.0	8.880279584692667E-6	1.989067817642241	0.0010504102137322184
46-47	0.09544841650469987	0.0	0.0	8.880279584692667E-6	2.0652282668889885	0.00106563355016312
48-49	0.12513075101653512	0.0	0.0	8.880279584692667E-6	2.141360172379928	0.001085297026386368
50-51	0.15992939518052257	0.0	0.0	8.880279584692667E-6	2.2173728284021585	0.0011030575855557534
52-53	0.20029026589295074	0.0	0.0	8.880279584692667E-6	2.2933892902584967	0.0011214524504097596
54-55	0.24615944717062682	0.0	0.0	8.880279584692667E-6	2.3691602758148873	0.0011404816209483867
56-57	0.29810908274107895	0.0	0.0	8.880279584692667E-6	2.4445646326855663	0.001151264817586942
58-59	0.35552072456180167	0.0	0.0	8.880279584692667E-6	2.5194330012527413	0.0011690253767563275
60-61	0.41926146850367174	0.0	0.0	1.0783196638555382E-5	2.594265214395892	0.0011848830188718501
62-63	0.4886754424887912	0.0	0.0	1.1417502323176286E-5	2.6692376090953447	0.0012039121894104772
64-65	0.5633960178314492	0.0	0.0	1.1417502323176286E-5	2.74382878607834	0.0012229413599491043
66-67	0.6436547161065322	0.0	0.0	1.1417502323176286E-5	2.817981657833262	0.001244507753226215
68-69	0.7294547088424636	0.0	0.0	1.1417502323176286E-5	2.892541753837711	0.0012565595612340124
70-71	0.8206437626749341	0.0	0.0	1.1417502323176286E-5	2.96661724029911	0.0012762230374572603
72-73	0.9171800134287589	0.0	0.0	1.1417502323176286E-5	3.0405385904791453	0.0013022295705267175
74-75	1.0184348641704783	0.0	0.0	1.1417502323176286E-5	3.114186554909109	0.001323795963803828
76-77	1.1239325856366273	0.0	0.0	1.1417502323176286E-5	3.1871875275407597	0.001357414165088736
78-79	1.2336059414246356	0.0	0.0	1.1417502323176286E-5	3.260570352194552	0.0013916666720582651
>>END_MODULE
