##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR546473_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78826347
Total Bases	7 Gbp
Sequences flagged as poor quality	0
Sequence length	90
%GC	18
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.20345902874327	38.0	37.0	38.0	36.0	38.0
2	37.139625600054764	38.0	37.0	38.0	36.0	38.0
3	37.12784566814951	38.0	37.0	38.0	36.0	38.0
4	37.07216287721668	38.0	37.0	38.0	36.0	38.0
5	37.096125195297965	38.0	37.0	38.0	35.0	38.0
6	37.15079855216429	38.0	37.0	38.0	36.0	38.0
7	37.11512870182859	38.0	37.0	38.0	36.0	38.0
8	37.07839755659361	38.0	37.0	38.0	35.0	38.0
9	37.06570919745907	38.0	37.0	38.0	35.0	38.0
10-11	37.027211688498014	38.0	37.0	38.0	35.0	38.0
12-13	36.960032633505136	38.0	37.0	38.0	35.0	38.0
14-15	36.93066056961894	38.0	37.0	38.0	35.0	38.0
16-17	36.84095264493229	38.0	37.0	38.0	35.0	38.0
18-19	36.78808582237104	38.0	37.0	38.0	35.0	38.0
20-21	36.70337184976998	38.0	36.5	38.0	35.0	38.0
22-23	36.58467800112569	38.0	36.0	38.0	34.0	38.0
24-25	36.57645418986624	38.0	36.0	38.0	34.0	38.0
26-27	36.60767417650345	38.0	36.0	38.0	35.0	38.0
28-29	36.592489253624805	38.0	36.0	38.0	34.0	38.0
30-31	36.57706760786466	38.0	36.0	38.0	34.5	38.0
32-33	36.553608706489975	38.0	36.0	38.0	34.5	38.0
34-35	36.4954629890435	38.0	36.0	38.0	34.0	38.0
36-37	36.36374768197744	38.0	36.0	38.0	34.0	38.0
38-39	36.27212228292147	38.0	36.0	38.0	34.0	38.0
40-41	36.1791164507471	38.0	36.0	38.0	34.0	38.0
42-43	36.077828616109784	37.0	36.0	38.0	33.0	38.0
44-45	35.99562123562569	37.0	36.0	38.0	33.0	38.0
46-47	35.82422625140805	37.0	36.0	38.0	33.0	38.0
48-49	35.684960956011324	37.0	36.0	38.0	32.5	38.0
50-51	35.36446907148951	37.0	35.5	38.0	32.0	38.0
52-53	35.38883738961035	37.0	35.0	38.0	32.0	38.0
54-55	35.262492152528644	37.0	35.0	38.0	32.0	38.0
56-57	35.16689646293009	37.0	35.0	38.0	32.0	38.0
58-59	35.01962237321489	37.0	35.0	38.0	31.0	38.0
60-61	34.84503188381925	37.0	35.0	38.0	31.0	38.0
62-63	34.66142530999185	36.0	34.5	38.0	30.0	38.0
64-65	34.52321725628107	36.0	34.0	38.0	30.0	38.0
66-67	34.257724368477966	36.0	34.0	38.0	30.0	38.0
68-69	34.02100861657334	36.0	34.0	37.5	29.0	38.0
70-71	33.478814152075316	36.0	33.5	37.0	28.0	38.0
72-73	32.891865361970915	35.0	32.0	37.0	27.0	38.0
74-75	32.656247586863316	35.0	32.0	37.0	26.0	38.0
76-77	33.130800784159135	36.0	33.0	37.0	28.0	38.0
78-79	33.016653943128944	36.0	33.0	37.0	27.5	38.0
80-81	33.29082380285871	36.0	33.5	37.5	27.5	38.0
82-83	33.46151338206755	36.0	34.0	38.0	28.0	38.0
84-85	33.17394711313972	36.0	33.0	37.5	26.5	38.0
86-87	32.48844965503729	36.0	33.0	37.0	25.0	38.0
88-89	32.11702034092738	36.0	32.0	37.0	23.5	38.0
90	31.832237081847772	36.0	32.0	37.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47226.0
3	3225.0
4	3146.0
5	6666.0
6	11445.0
7	15140.0
8	22464.0
9	30084.0
10	37694.0
11	55230.0
12	60787.0
13	87671.0
14	93861.0
15	116984.0
16	112606.0
17	137035.0
18	146110.0
19	177325.0
20	187374.0
21	220083.0
22	237273.0
23	281682.0
24	322949.0
25	393480.0
26	472416.0
27	583103.0
28	712890.0
29	891010.0
30	1120233.0
31	1444309.0
32	1915856.0
33	2694133.0
34	4281776.0
35	8378699.0
36	2.685821E7
37	2.6666159E7
38	13.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.277202549007395	34.63772034678302	48.36781533224912	0.7172617719604618
2	18.037033809589193	34.86309695533034	46.678737612920436	0.4211316221600317
3	17.019829676998732	33.376294857352704	49.20394700010645	0.3999284655421112
4	17.57728867051756	32.933252253310435	49.113639500870036	0.375819575301966
5	17.696983353575163	33.06104186409186	48.90146698491678	0.34050779741619225
6	17.663251602919008	32.99619478751185	49.001486774466414	0.3390668351027354
7	17.86856874136258	33.00742199812964	48.81551849662652	0.3084907638812693
8	17.19643940876773	32.97199221980945	49.525924371454124	0.3056439999686907
9	17.27012923737288	32.530015630433816	49.892942520855364	0.30691261133793246
10-11	17.341427099241326	32.80412766051432	49.56037668471431	0.2940685555300438
12-13	17.260719946847214	32.8533497816409	49.59428412939141	0.29164614212047657
14-15	17.326039634817093	32.77645965290832	49.60948018760256	0.28802052467202705
16-17	17.45013483202999	32.6170195277741	49.64247434697619	0.2903712932197129
18-19	17.52972698477169	32.49944374037963	49.68075727882475	0.29007199602393147
20-21	17.59588453337816	32.43008394135022	49.68391596276813	0.2901155625034863
22-23	17.689115417209425	32.40996059350562	49.61118203282971	0.2897419564552446
24-25	17.784802332651545	32.37122798548561	49.55103780211964	0.2929318797432031
26-27	17.86872437293998	32.38314319428177	49.454899254114856	0.2932331786633875
28-29	17.910764198923783	32.36269442685896	49.431845897866246	0.2946954763510139
30-31	17.92175254953372	32.33780001580817	49.4439912716723	0.2964561629858035
32-33	17.96579731387755	32.33512459330656	49.40070863436191	0.29836945845398105
34-35	18.02603423877266	32.32358239459903	49.35131275345877	0.2990706131695427
36-37	18.053354808911976	32.28011385725232	49.364140442964136	0.3023908908715622
38-39	18.07750430909086	32.25868123577714	49.35911184347238	0.3047026116596242
40-41	18.105396592723014	32.240481372124385	49.3453777947591	0.3087442403935027
42-43	18.117587628453062	32.222852210427334	49.348601841184944	0.31095831993466194
44-45	18.15242586253791	32.21565158551937	49.318184493262834	0.31373805867989074
46-47	18.188107405599247	32.205654442628685	49.28810597026762	0.3181321815044486
48-49	18.214197320346734	32.18110778813381	49.27062733853219	0.33406755298727475
50-51	18.174463686536228	32.13792000958658	49.18317925586295	0.5044370480142434
52-53	18.260846616248045	32.14837577793713	49.252590036333416	0.33818756948140083
54-55	18.27460527080524	32.12832836598009	49.24834298201106	0.34872338120360236
56-57	18.30447613678668	32.13558087867031	49.20327284973851	0.3566701348044989
58-59	18.33558139996809	32.11842210004611	49.17814906154987	0.3678474384359349
60-61	18.34808758939268	32.08729774195838	49.18426103585865	0.380353632790293
62-63	18.37783888332337	32.078104042007645	49.15261644637075	0.3914406282982289
64-65	18.40696059665566	32.08017660749899	49.10612409764386	0.40673869820149233
66-67	18.438190605897933	32.053245196975574	49.08459572675747	0.42396847036902324
68-69	18.473418127791188	32.036393688144976	49.04344332835491	0.4467448557089216
70-71	18.49382683349078	32.0303851131413	49.014683305601295	0.46110474776662097
72-73	18.507219938146765	32.00197847112033	49.00728162261787	0.4835199681150447
74-75	18.57683596787502	31.987661001731816	48.92960463436574	0.5058983960274284
76-77	18.417593718101006	31.998824176562074	49.059136844739875	0.5244452605970399
78-79	18.46235843206284	31.991171740218537	49.00107627809523	0.545393549623387
80-81	18.50175055702917	31.979346180700375	48.94550294969023	0.5734003125802267
82-83	18.512705980774886	31.960624616829236	48.92587211587468	0.6007972865211996
84-85	18.525838254356554	31.946224904280353	48.89486782570053	0.6330690156625616
86-87	18.551639769637077	31.921753654610978	48.864048035233786	0.6625585405181597
88-89	18.57504137656312	31.906232238659634	48.823118287532054	0.695608097245195
90	18.555714657707416	31.872072905366732	48.85082827735209	0.7213841595737531
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12025.0
1	12104.0
2	15168.5
3	25803.0
4	52673.5
5	116841.0
6	257029.0
7	528121.0
8	990411.0
9	1681157.5
10	2085464.0
11	2585765.0
12	3629449.0
13	4683379.0
14	5603371.0
15	6268591.0
16	6612906.0
17	6611381.0
18	6278803.0
19	5720272.0
20	5404254.0
21	5035618.5
22	4305786.5
23	3600253.5
24	2948575.0
25	2378565.5
26	1900825.5
27	1510427.0
28	1193092.5
29	941051.5
30	831019.0
31	743671.0
32	586103.0
33	457539.5
34	352125.5
35	271518.5
36	212499.5
37	165783.0
38	128806.0
39	99296.0
40	85529.0
41	74597.5
42	55088.0
43	40454.0
44	29728.0
45	21984.5
46	16545.5
47	12521.0
48	9909.5
49	13576.0
50	18195.0
51	14081.0
52	8081.5
53	5146.0
54	3447.5
55	2409.5
56	1782.5
57	1331.0
58	989.5
59	772.0
60	682.0
61	615.0
62	475.5
63	345.5
64	256.0
65	207.0
66	157.5
67	113.0
68	91.5
69	75.5
70	69.0
71	63.5
72	50.5
73	47.0
74	38.0
75	25.5
76	24.5
77	20.5
78	18.5
79	17.0
80	15.0
81	13.5
82	12.5
83	16.5
84	15.5
85	11.5
86	10.5
87	11.5
88	12.5
89	13.0
90	15.0
91	12.5
92	14.0
93	15.0
94	15.0
95	22.5
96	34.0
97	43.0
98	50.5
99	95.0
100	134.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03789469021062209
2	2.0297781907868952E-4
3	0.0
4	0.13834587564992706
5	0.0014373366813509702
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0017874734192617095
16-17	0.001565466429644393
18-19	9.438468587159061E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.2686113692418093E-6
28-29	0.00438178366936121
30-31	2.7275144438698902E-5
32-33	1.0402613227782839E-4
34-35	0.0014589030746280807
36-37	0.038677423425444286
38-39	0.031616332544244376
40-41	0.09278242971223823
42-43	0.039607315559098535
44-45	0.03386494619622548
46-47	0.03707643587746112
48-49	0.014145016767046176
50-51	0.1686993055760912
52-53	0.01590775226460767
54-55	0.07363147248216387
56-57	0.10814087325396418
58-59	0.10922109583487358
60-61	0.1555520516509537
62-63	0.10090407969812429
64-65	0.09973188279294486
66-67	0.07715694347728685
68-69	0.08977645507281976
70-71	0.09066131150286591
72-73	0.022859742568052788
74-75	0.02839279105500094
76-77	0.07753752688805939
78-79	0.08671783306157775
80-81	0.050558603204078456
82-83	0.06378007089431659
84-85	0.12117204923881605
86-87	0.12956771928045835
88-89	0.16175746416360004
90	0.18513987461578044
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
90	7.8826347E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.06448831808676
#Duplication Level	Percentage of total
1	82.61900845179495
2	7.902251872803974
3	1.95284441029922
4	1.012050143018902
5	0.744513931100107
6	0.64085264673355
7	0.4799455590211143
8	0.37297317714539374
9	0.34311436655863525
>10	3.098254041178193
>50	0.39382759087659197
>100	0.3034194242588604
>500	0.07495154014230235
>1k	0.04653443893956973
>5k	0.0
>10k+	0.015458406128540724
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.074445476967238E-6	0.0	0.0	0.0	0.07074918745124648	0.001019963540870415
2	6.3430568462090476E-6	0.0	0.0	0.0	0.07598474657210742	0.0015286766999363804
3	1.0148890953934476E-5	0.0	0.0	0.0	0.07892665633737918	0.0020259723566791697
4	1.1417502323176286E-5	0.0	0.0	0.0	0.08186476026854321	0.002568938022714664
5	1.6491947800143523E-5	0.0	0.0	0.0	0.08482189337024587	0.0032057809300740526
6	2.283500464635257E-5	0.0	0.0	0.0	0.08761283838257784	0.00396821636298838
7	2.9178061492561617E-5	0.0	0.0	0.0	0.0905940751002961	0.004848632653242196
8	3.4252506969528854E-5	0.0	0.0	0.0	0.09330129176225811	0.005802628402912037
9	3.552111833877067E-5	0.0	0.0	0.0	0.09615186150894446	0.007023032540122657
10-11	4.757292634656785E-5	0.0	0.0	0.0	0.10031861555121918	0.009234856462395753
12-13	6.469917983133229E-5	0.0	0.0	0.0	0.105884013628083	0.012875771092119745
14-15	1.1988377439335099E-4	0.0	0.0	0.0	0.11112528150010553	0.017307664910566005
16-17	1.763369803246115E-4	0.0	0.0	0.0	0.11593775872932435	0.02174717039722772
18-19	1.9219462244013413E-4	0.0	0.0	0.0	0.12055804133610301	0.026300216601436575
20-21	0.0024801352268677378	0.0	0.0	0.0	0.12504258252637282	0.0309312824048538
22-23	0.002617145254745853	0.0	0.0	0.0	0.12944149244921876	0.0355978693266098
24-25	0.0026900904084772572	0.0	0.0	0.0	0.13373954776821004	0.04020673443106529
26-27	0.002875941974071182	0.0	0.0	0.0	0.13803887169857054	0.044977347485099115
28-29	0.003381483604714043	0.0	0.0	0.0	0.1424130436997163	0.04983930055771835
30-31	0.0044261850672846735	0.0	0.0	0.0	0.1468462061295318	0.05478878781481527
32-33	0.00640775602604038	0.0	0.0	0.0	0.1511670964531694	0.0596659642238654
34-35	0.010141279285719024	0.0	0.0	0.0	0.15550574733597639	0.06468903094037835
36-37	0.016228710941025848	0.0	0.0	0.0	0.15995540171359204	0.06984783907340017
38-39	0.025143243032688042	0.0	6.343056846209048E-7	0.0	0.16445897207440047	0.07504343693613
40-41	0.03715064964256177	0.0	1.2686113692418096E-6	0.0	0.16898030299437827	0.08016608964512842
42-43	0.05261755945635791	0.0	1.2686113692418096E-6	0.0	0.1734997309973022	0.08526527304379589
44-45	0.07226327512043657	0.0	1.2686113692418096E-6	0.0	0.1779779291307258	0.09037714255615575
46-47	0.09676142927186515	0.0	1.2686113692418096E-6	0.0	0.18246310462668022	0.09570721322402521
48-49	0.12636701279586127	0.0	1.2686113692418096E-6	0.0	0.1870205909706814	0.10101444888724832
50-51	0.16099756595342418	0.0	1.2686113692418096E-6	0.0	0.19163009038082152	0.10664962058942043
52-53	0.20142821029116065	0.0	1.2686113692418096E-6	0.0	0.1962529102103387	0.11247508399697884
54-55	0.24742869284555327	0.0	1.2686113692418096E-6	0.0	0.20080849363728603	0.11822506502806733
56-57	0.2994487363469983	0.0	1.2686113692418096E-6	0.0	0.20535139095054095	0.12406575177205661
58-59	0.35697645610800666	0.0	1.2686113692418096E-6	0.0	0.20982514894417217	0.12991087865583825
60-61	0.42060239072096034	0.0	1.2686113692418096E-6	0.0	0.21437756084269644	0.13573317053497352
62-63	0.49013815139752703	0.0	1.2686113692418096E-6	0.0	0.21889698884562037	0.14159288644950146
64-65	0.5646392569733061	0.0	1.2686113692418096E-6	0.0	0.22352868895472222	0.14751349570975297
66-67	0.6445896826856634	0.0	1.2686113692418096E-6	0.0	0.2281134504431621	0.15307128211840137
68-69	0.7299926000630221	0.0	1.2686113692418096E-6	0.0	0.23277876875354886	0.1586347772782113
70-71	0.8205574971018257	0.0	1.2686113692418096E-6	0.0	0.23752400958019787	0.16435875177623036
72-73	0.9160909105682646	0.0	2.537222738483619E-6	0.0	0.24226227304431602	0.17010429266752652
74-75	1.0159756610312032	0.0	2.537222738483619E-6	0.0	0.24692441982627966	0.17576991104256043
76-77	1.1198032556297453	0.0	2.537222738483619E-6	0.0	0.2515618286865431	0.18119449325743842
78-79	1.2275552994990369	0.0	2.537222738483619E-6	0.0	0.256317218404146	0.18682205329139506
>>END_MODULE
