Bismark report for: SRR546472_val_1.fq.gz and SRR546472_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_03/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 67603212 Number of paired-end alignments with a unique best hit: 38591038 Mapping efficiency: 57.1% Sequence pairs with no alignments under any condition: 19264242 Sequence pairs did not map uniquely: 9747932 Sequence pairs which were discarded because genomic sequence could not be extracted: 8 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 19280123 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 19310907 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1111132155 Total methylated C's in CpG context: 15190491 Total methylated C's in CHG context: 938643 Total methylated C's in CHH context: 3575331 Total methylated C's in Unknown context: 26115 Total unmethylated C's in CpG context: 126068858 Total unmethylated C's in CHG context: 194670823 Total unmethylated C's in CHH context: 770688009 Total unmethylated C's in Unknown context: 2502993 C methylated in CpG context: 10.8% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in Unknown context (CN or CHN): 1.0% Bismark completed in 0d 6h 55m 34s