##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR546472_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68365315
Total Bases	6.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90
%GC	18
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.045891721555	38.0	38.0	38.0	36.0	38.0
2	37.00830130015491	38.0	38.0	38.0	36.0	38.0
3	36.99896987236876	38.0	38.0	38.0	36.0	38.0
4	36.98275113630355	38.0	38.0	38.0	36.0	38.0
5	36.99414423820032	38.0	38.0	38.0	36.0	38.0
6	37.017510078027136	38.0	38.0	38.0	36.0	38.0
7	36.966914991907814	38.0	38.0	38.0	36.0	38.0
8	36.923747063843706	38.0	38.0	38.0	36.0	38.0
9	36.92443720913156	38.0	38.0	38.0	36.0	38.0
10-11	36.91538386095347	38.0	38.0	38.0	36.0	38.0
12-13	36.88022617902075	38.0	38.0	38.0	35.0	38.0
14-15	36.77609334499519	38.0	38.0	38.0	35.0	38.0
16-17	36.79239695597102	38.0	38.0	38.0	35.0	38.0
18-19	36.7400350821173	38.0	37.0	38.0	35.0	38.0
20-21	36.695375432702974	38.0	37.0	38.0	35.0	38.0
22-23	36.6364350036272	38.0	37.0	38.0	35.0	38.0
24-25	36.571550741776	38.0	37.0	38.0	35.0	38.0
26-27	36.51496289456138	38.0	37.0	38.0	35.0	38.0
28-29	36.443658183978236	38.0	37.0	38.0	35.0	38.0
30-31	36.38033029614506	38.0	37.0	38.0	35.0	38.0
32-33	36.28522447384321	38.0	37.0	38.0	34.0	38.0
34-35	36.20355035298236	38.0	37.0	38.0	34.0	38.0
36-37	36.217572968105245	38.0	37.0	38.0	34.0	38.0
38-39	36.20383089729053	38.0	37.0	38.0	34.0	38.0
40-41	36.18415390172633	38.0	37.0	38.0	34.0	38.0
42-43	36.10721275839949	38.0	37.0	38.0	33.5	38.0
44-45	36.053950896006256	38.0	37.0	38.0	33.0	38.0
46-47	35.750016495938034	38.0	36.0	38.0	33.0	38.0
48-49	35.712662129911934	38.0	36.0	38.0	33.0	38.0
50-51	35.568108367525255	37.5	36.0	38.0	32.5	38.0
52-53	35.41244273503311	37.0	36.0	38.0	32.0	38.0
54-55	35.41160976878407	37.0	36.0	38.0	32.0	38.0
56-57	35.91450049634087	38.0	36.5	38.0	33.0	38.0
58-59	35.83988183189092	38.0	36.0	38.0	33.0	38.0
60-61	35.80479341753929	38.0	36.0	38.0	33.0	38.0
62-63	35.738171885845915	38.0	36.0	38.0	33.0	38.0
64-65	35.681778318435306	38.0	36.0	38.0	32.0	38.0
66-67	35.64384719795411	38.0	36.0	38.0	32.5	38.0
68-69	35.55072978161513	38.0	36.0	38.0	32.0	38.0
70-71	35.5000213704859	38.0	36.0	38.0	32.0	38.0
72-73	35.39585053473388	38.0	36.0	38.0	32.0	38.0
74-75	35.32706846300643	38.0	36.0	38.0	32.0	38.0
76-77	35.244860891813346	38.0	36.0	38.0	32.0	38.0
78-79	35.131179231749314	38.0	36.0	38.0	31.5	38.0
80-81	35.03237955533446	38.0	36.0	38.0	31.0	38.0
82-83	34.88256753442882	38.0	35.5	38.0	30.5	38.0
84-85	34.74898014439047	37.0	35.0	38.0	30.0	38.0
86-87	34.53839961097232	37.0	35.0	38.0	30.0	38.0
88-89	34.31490470716035	37.0	35.0	38.0	30.0	38.0
90	34.11147867891781	37.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	484068.0
3	63985.0
4	24696.0
5	31075.0
6	26657.0
7	40823.0
8	31821.0
9	47098.0
10	41660.0
11	58478.0
12	52958.0
13	71910.0
14	62553.0
15	76096.0
16	67366.0
17	80260.0
18	73897.0
19	90177.0
20	90437.0
21	115697.0
22	128904.0
23	162457.0
24	191736.0
25	238297.0
26	278229.0
27	345883.0
28	414488.0
29	522415.0
30	649117.0
31	830231.0
32	1082859.0
33	1504215.0
34	2227149.0
35	3782617.0
36	8654388.0
37	4.5715742E7
38	4876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.43719829943196264	47.01763059194678	32.12894501635852	20.416226092262736
2	0.43461937380162285	52.2464753013106	31.090977427578466	16.22792789730931
3	0.36752189643132116	48.69754160890529	32.981414280533485	17.9535222141299
4	0.3534832623879679	49.20891219923152	32.477225214333515	17.960379324047
5	0.34346187239234777	49.834715806356286	32.40270778174502	17.41911453950635
6	0.33697520657707214	50.10355714440381	31.534157292860797	18.025310356158325
7	0.3411354301997907	50.162296050129655	32.05989729228043	17.43667122739012
8	0.3155562530026203	49.55129805802084	32.29807912847914	17.8350665604974
9	0.3188258839273221	49.056826237041534	32.65252936832906	17.97181851070208
10-11	0.3244120777547971	49.38299835172678	32.50772727059919	17.784862299919226
12-13	0.3105312205581907	49.4438163966159	32.54548306791335	17.70016931491255
14-15	0.32064609547251316	49.20993207249544	32.75012171390825	17.719300118123797
16-17	0.30937002792719903	49.14228718891411	32.72013539513542	17.82820738802326
18-19	0.31467436600694065	49.08450326833344	32.64737851043837	17.953443855221245
20-21	0.3107717028972838	49.12055334260724	32.563172164437745	18.005502790057736
22-23	0.3070327076072136	49.08523044752241	32.58067041409979	18.02706643077058
24-25	0.3095860537281276	49.13763542919462	32.523894322210126	18.028884194867135
26-27	0.31291983736211176	49.208148391542636	32.45946743601324	18.01946433508201
28-29	0.30821338041014634	49.233805268208684	32.42538933949004	18.032592011891126
30-31	0.30733004357202237	49.203145455040904	32.4079875334649	18.081536967922176
32-33	0.30818571022761676	49.21917574389105	32.413016949776605	18.05962159610473
34-35	0.3042687727099239	49.21440344537564	32.41413710432189	18.067190677592553
36-37	0.31977187100734594	49.21654185776763	32.37681811871197	18.086868152513063
38-39	0.3296794919443841	49.22365518352406	32.36373103668275	18.082934287848804
40-41	0.335012735257483	49.23016247561776	32.34624076778576	18.088584021339003
42-43	0.34727275144297726	49.203971397738925	32.34705155354977	18.101704297268324
44-45	0.34707160681451776	49.22129512104702	32.339020578485446	18.092612693653017
46-47	0.370988811933647	49.22594506566762	32.298605161516214	18.10446096088252
48-49	0.39313486429705685	49.226371541786726	32.26269602346944	18.117797570446786
50-51	0.4027425216317422	49.2310077705917	32.29444141659395	18.071808291182606
52-53	0.4149322701140888	49.2035928266193	32.31795489338031	18.063520009886304
54-55	0.44067644464718064	49.188115567963706	32.28105657970596	18.09015140768315
56-57	0.45973899354241726	49.20172052644093	32.256162121239704	18.08237835877695
58-59	0.48789813281431266	49.18986835210438	32.2534259547953	18.068807560286015
60-61	0.5255246690346096	49.14947731494128	32.258446126840305	18.066551889183806
62-63	0.5548173618530312	49.14029590729996	32.27076048069006	18.034126250156948
64-65	0.5899263553516452	49.12941688361078	32.24290774181202	18.03774901922555
66-67	0.6244006945820076	49.117752996521574	32.21776494199532	18.040081366901102
68-69	0.6594771815201217	49.12181605891579	32.193909118747314	18.024797640816782
70-71	0.7057328648045866	49.08235219964417	32.21156285736456	18.000352078186683
72-73	0.7446842623570537	49.05965923089766	32.21210438563524	17.983552121110048
74-75	0.7887393846280917	49.076067639918406	32.17607084297698	17.95912213247652
76-77	0.833254942254463	49.02043534998922	32.17806026790473	17.968249439851586
78-79	0.8810675840713511	49.00205440724952	32.14279826432628	17.974079744352856
80-81	0.9287026569660252	48.999929987138415	32.13724619197279	17.93412116392277
82-83	0.9756626045316935	48.978263777192346	32.126936725699075	17.919136892576887
84-85	1.02417070182347	48.925259571860465	32.133437394868544	17.917132331447526
86-87	1.0722267587369445	48.901652461858916	32.136872281055716	17.889248498348422
88-89	1.1176501183310479	48.875785389364005	32.11904230236179	17.887522189943155
90	1.1490121802500348	48.83394197890204	32.123441154141005	17.893604686706915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	287354.0
1	174053.5
2	54493.5
3	53727.5
4	81047.0
5	152717.0
6	293882.0
7	537893.5
8	921415.0
9	1467971.0
10	1783696.0
11	2173668.0
12	2997215.5
13	3860093.5
14	4650242.5
15	5251987.0
16	5585616.5
17	5627052.0
18	5398969.0
19	4960722.5
20	4699263.0
21	4397131.0
22	3781557.0
23	3173660.5
24	2613971.5
25	2119569.0
26	1701104.5
27	1355299.5
28	1071055.5
29	843355.5
30	743391.0
31	664943.0
32	522217.0
33	405760.0
34	311530.5
35	238197.5
36	185950.0
37	146127.0
38	113539.5
39	87218.0
40	74626.0
41	64441.0
42	46155.0
43	32342.5
44	23162.0
45	17082.5
46	13356.5
47	10868.5
48	8549.5
49	8984.5
50	10366.0
51	7455.0
52	3757.5
53	2566.0
54	1877.0
55	1382.5
56	996.5
57	761.5
58	600.5
59	424.5
60	350.0
61	300.5
62	217.5
63	166.0
64	134.0
65	110.5
66	96.0
67	79.0
68	61.0
69	62.5
70	70.0
71	61.0
72	51.0
73	52.0
74	46.5
75	38.5
76	43.5
77	41.0
78	33.0
79	27.5
80	22.0
81	23.0
82	25.5
83	22.0
84	16.5
85	18.5
86	18.5
87	17.0
88	14.0
89	10.0
90	10.0
91	10.5
92	11.0
93	11.0
94	13.5
95	13.5
96	11.5
97	14.0
98	17.0
99	21.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5741186155581964
2	0.4522966068393015
3	0.4399877335458778
4	0.4117131618570031
5	0.4500644807970241
6	0.48189494921511006
7	0.5030957584266231
8	0.4871929574229271
9	0.4919658455460931
10-11	0.4909426658825458
12-13	0.5007861076921828
14-15	0.5048627363159227
16-17	0.5424512415396608
18-19	0.5506586197986508
20-21	0.5462455632655243
22-23	0.5211326825598623
24-25	0.5208993771183531
26-27	0.5170567853011429
28-29	0.5201665493679068
30-31	0.5062428221094278
32-33	0.5242870598928712
34-35	0.5299178391849727
36-37	0.5310967995978663
38-39	0.5363779864102141
40-41	0.5346453826768736
42-43	0.5307647598786022
44-45	0.5917554830252738
46-47	0.6179017825047687
48-49	0.5900587161779332
50-51	0.5528490580347651
52-53	0.5977914385386801
54-55	0.5844725501520763
56-57	0.5690422109515622
58-59	0.5398227156563237
60-61	0.5696433929983356
62-63	0.557374744781034
64-65	0.5789690283735254
66-67	0.557894013945522
68-69	0.5319137343256591
70-71	0.5356729505305431
72-73	0.5571026769934433
74-75	0.560836295422613
76-77	0.5532037700696618
78-79	0.5829103544684904
80-81	0.5944139948744477
82-83	0.5623282800642402
84-85	0.5435087953591672
86-87	0.5476848896256823
88-89	0.5144092439272752
90	0.5401218439496696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
90	6.8365315E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.0672999357925
#Duplication Level	Percentage of total
1	85.46097616135977
2	6.67982654547416
3	1.6607190516005517
4	0.9217488366160782
5	0.6978099862990228
6	0.4995158900586298
7	0.35691659454554453
8	0.3021513991963713
9	0.254314104476746
>10	2.3069720776356055
>50	0.2436959619812802
>100	0.21920516664228484
>500	0.012174205244619294
>1k	0.034358293757583536
>5k	0.009643895015815413
>10k+	0.3399718300958783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	218411	0.3194763309435494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.850920163243598E-6	0.0	0.0	0.0	0.22251780745835809	4.095644114270519E-5
2	1.3164570367298096E-5	0.0	0.0	0.0	0.2805121281164286	5.850920163243598E-5
3	1.6090030448919895E-5	0.0	0.0	0.0	0.32450519682385726	8.191288228541038E-5
4	2.0478220571352594E-5	0.0	0.0	1.4627300408108995E-6	0.36986006719928083	9.946564277514116E-5
5	2.3403680652974392E-5	0.0	0.0	1.4627300408108995E-6	0.41669229491592336	1.1409294318325016E-4
6	2.486641069378529E-5	0.0	0.0	1.4627300408108995E-6	0.46228997847812153	1.2872024359135916E-4
7	2.925460081621799E-5	0.0	0.0	1.4627300408108995E-6	0.5067057761673445	1.3895935387703545E-4
8	3.364279093865069E-5	0.0	0.0	1.4627300408108995E-6	0.5495374372223693	1.5943757444838804E-4
9	3.510552097946159E-5	0.0	0.0	1.4627300408108995E-6	0.5931779879899625	1.6675122465244254E-4
10-11	4.241917118351608E-5	0.0	0.0	1.4627300408108995E-6	0.6591310228000851	1.996626505706878E-4
12-13	6.362875677527413E-5	0.0	0.0	2.1940950612163492E-6	0.745289479028949	2.2745452134609486E-4
14-15	2.47201376897042E-4	0.0	0.0	2.925460081621799E-6	0.8287938115987616	2.4647001187663655E-4
16-17	4.395503772636753E-4	0.0	0.0	2.925460081621799E-6	0.9121482143393913	2.574404871827183E-4
18-19	5.031791340389494E-4	0.0	0.0	5.119555142838149E-6	0.9958405077194481	2.7572461269285455E-4
20-21	0.008592076259723223	0.0	0.0	5.850920163243598E-6	1.0792973015629344	2.881578180397472E-4
22-23	0.008841471731681483	0.0	0.0	5.850920163243598E-6	1.1626224497027478	3.0424784848866713E-4
24-25	0.009009685686374735	0.0	0.0	5.850920163243598E-6	1.2461816346490906	3.1960651391718155E-4
26-27	0.009353427245965298	0.0	0.0	5.850920163243598E-6	1.329162309864293	3.3423381432529055E-4
28-29	0.01026397669637008	0.0	0.0	5.850920163243598E-6	1.4113436031121922	3.4447292461096686E-4
30-31	0.012097508802526545	0.0	0.0	5.850920163243598E-6	1.493434207097561	3.5910022501907586E-4
32-33	0.01545593697622837	0.0	0.0	5.850920163243598E-6	1.575257862850482	3.7080206534556304E-4
34-35	0.020879739967555183	0.0	0.0	5.850920163243598E-6	1.6558557508292033	3.810411756312393E-4
36-37	0.02901690718458622	0.0	0.0	5.850920163243598E-6	1.7362481252371906	3.8908619085569927E-4
38-39	0.04048324797450286	0.0	0.0	5.850920163243598E-6	1.816275548499996	3.920116509373211E-4
40-41	0.055625429356977285	0.0	0.0	5.850920163243598E-6	1.8965618749800246	3.956684760393483E-4
42-43	0.07495248138621172	0.0	0.0	5.850920163243598E-6	1.9757650498648327	3.9786257110056465E-4
44-45	0.09888859577404127	0.0	0.0	5.850920163243598E-6	2.0544665083456426	4.00056666161781E-4
46-47	0.12807737373842276	0.0	0.0	5.850920163243598E-6	2.1331292044803716	4.0371349126380827E-4
48-49	0.1626672823784985	0.0	0.0	5.850920163243598E-6	2.2113311406522445	4.0663895134543E-4
50-51	0.20271829362594174	0.0	0.0	5.850920163243598E-6	2.289937521680402	4.102957764474573E-4
52-53	0.24840374099058857	0.0	0.0	5.850920163243598E-6	2.368411525639866	4.132212365290791E-4
54-55	0.299913048012724	0.0	0.0	5.850920163243598E-6	2.4471714933223083	4.1834079167191727E-4
56-57	0.3571489431446341	0.0	0.0	5.850920163243598E-6	2.5252907852468756	4.2272898179434996E-4
58-59	0.4205677981590519	0.0	0.0	5.850920163243598E-6	2.6035344092249115	4.3004263199840446E-4
60-61	0.48949310041210226	0.0	0.0	5.850920163243598E-6	2.6810700718631955	4.3662491718205354E-4
62-63	0.5642671287333351	0.0	0.0	5.850920163243598E-6	2.758975073836784	4.4759539248813527E-4
64-65	0.6440766052200593	0.0	0.0	5.850920163243598E-6	2.8370256174494335	4.5929723281462246E-4
66-67	0.7293874093902735	0.0	0.0	5.850920163243598E-6	2.9146527007152674	4.717304381615151E-4
68-69	0.8194652507634903	0.0	0.0	5.850920163243598E-6	2.9921561832926535	4.841636435084077E-4
70-71	0.9141777522710164	0.0	0.0	5.850920163243598E-6	3.068937077229879	5.039104990593548E-4
72-73	1.0132016505738326	0.0	0.0	5.850920163243598E-6	3.145706269326778	5.207318945286803E-4
74-75	1.1157397577996973	0.0	0.0	5.850920163243598E-6	3.2227204687055124	5.404787500796274E-4
76-77	1.221392017282448	0.0	0.0	5.850920163243598E-6	3.2994011656349422	5.521805904061146E-4
78-79	1.3293195533436801	0.0	0.0	5.850920163243598E-6	3.37845953024571	5.733901759978725E-4
>>END_MODULE
