##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR546472_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68365315
Total Bases	6.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90
%GC	18
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.17412325241243	38.0	37.0	38.0	35.0	38.0
2	37.07327350133617	38.0	37.0	38.0	35.0	38.0
3	37.0525402830368	38.0	37.0	38.0	35.0	38.0
4	36.96423206709426	38.0	37.0	38.0	35.0	38.0
5	37.03480121462177	38.0	37.0	38.0	35.0	38.0
6	37.083385017680385	38.0	37.0	38.0	35.0	38.0
7	37.063865265595574	38.0	37.0	38.0	35.0	38.0
8	37.01253621079637	38.0	37.0	38.0	35.0	38.0
9	37.015190495355725	38.0	37.0	38.0	35.0	38.0
10-11	36.96116515370404	38.0	37.0	38.0	35.0	38.0
12-13	36.901443297672216	38.0	37.0	38.0	35.0	38.0
14-15	36.872655505207575	38.0	37.0	38.0	35.0	38.0
16-17	36.80813046059979	38.0	37.0	38.0	35.0	38.0
18-19	36.77203658755906	38.0	37.0	38.0	35.0	38.0
20-21	36.69197441714413	38.0	36.5	38.0	35.0	38.0
22-23	36.59431326397019	38.0	36.0	38.0	34.0	38.0
24-25	36.525483836357665	38.0	36.0	38.0	34.0	38.0
26-27	36.30245988773693	38.0	36.0	38.0	34.0	38.0
28-29	36.18757149001654	38.0	36.0	38.0	33.0	38.0
30-31	36.078704288863435	37.5	36.0	38.0	33.0	38.0
32-33	35.99934479201917	37.0	36.0	38.0	33.0	38.0
34-35	36.02780877262103	37.0	36.0	38.0	33.0	38.0
36-37	36.10646826537697	38.0	36.0	38.0	33.0	38.0
38-39	36.13114003058422	38.0	36.0	38.0	33.0	38.0
40-41	36.14542537396339	38.0	36.0	38.0	33.5	38.0
42-43	36.10248630464147	38.0	36.0	38.0	33.0	38.0
44-45	36.03646509929779	38.0	36.0	38.0	33.0	38.0
46-47	35.92156455360441	38.0	36.0	38.0	33.0	38.0
48-49	35.829853193830814	38.0	36.0	38.0	33.0	38.0
50-51	35.760132042103514	37.0	36.0	38.0	32.5	38.0
52-53	35.64852538893443	37.0	36.0	38.0	32.0	38.0
54-55	35.56196488672656	37.0	36.0	38.0	32.0	38.0
56-57	35.41891312868229	37.0	36.0	38.0	32.0	38.0
58-59	35.297292559830964	37.0	35.0	38.0	32.0	38.0
60-61	35.215763805081565	37.0	35.0	38.0	31.5	38.0
62-63	35.0498774707613	37.0	35.0	38.0	31.0	38.0
64-65	34.92762372995722	37.0	35.0	38.0	30.0	38.0
66-67	34.770174334748546	37.0	35.0	38.0	30.0	38.0
68-69	34.63977978452962	37.0	35.0	38.0	30.0	38.0
70-71	34.23130102596616	36.5	34.5	38.0	29.5	38.0
72-73	33.80807084703699	36.0	34.0	37.5	28.0	38.0
74-75	33.65271625677436	36.0	33.0	37.0	28.0	38.0
76-77	33.44307650743656	36.0	34.0	37.0	28.0	38.0
78-79	33.382995434161316	36.0	33.5	37.0	27.5	38.0
80-81	33.597129597077114	36.5	33.5	37.5	27.5	38.0
82-83	33.716105498819104	36.5	34.0	38.0	27.5	38.0
84-85	33.50668385715768	36.0	34.0	38.0	26.5	38.0
86-87	33.095074483310725	36.0	33.5	38.0	25.5	38.0
88-89	32.731946323950964	36.0	33.0	38.0	25.0	38.0
90	32.44167518280286	36.0	33.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12859.0
3	2090.0
4	3443.0
5	5460.0
6	8053.0
7	12687.0
8	20968.0
9	31370.0
10	40315.0
11	62905.0
12	55448.0
13	76882.0
14	71284.0
15	89983.0
16	88880.0
17	114703.0
18	109427.0
19	131444.0
20	141172.0
21	171307.0
22	192878.0
23	238072.0
24	280436.0
25	352082.0
26	427747.0
27	540152.0
28	669151.0
29	845812.0
30	1063836.0
31	1373326.0
32	1807099.0
33	2502806.0
34	3812980.0
35	6541887.0
36	1.4672874E7
37	3.1792962E7
38	535.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.897650844752864	32.35568675656511	49.932365762478895	0.8142966362031212
2	18.463899420342024	34.64932765979356	46.4736438353279	0.41312908453650804
3	16.981960808635197	32.69940612428978	49.92236779717902	0.3962652698959992
4	17.725853327587945	32.81745326524438	49.076595674456556	0.3800977327111201
5	17.834630031325094	32.99786009908679	48.82495019587053	0.3425596737175862
6	17.940929548850903	33.090673245636324	48.617706215498316	0.350690990014454
7	18.232326117890718	32.90880811283921	48.54302578058809	0.3158399886819799
8	17.48734720230573	32.89177121468686	49.30289284851536	0.3179887344920447
9	17.803944880528963	32.11651697940688	49.75372379985377	0.325814340210383
10-11	17.628608161901983	32.67544880031636	49.392062334533236	0.30388070324842353
12-13	17.29328900188641	33.09177102453196	49.31651598475045	0.2984239888311785
14-15	17.378925263490704	32.71053969399541	49.61255279815503	0.2979822443588536
16-17	17.49157156666359	32.564097013229585	49.641116990391986	0.3032144297148342
18-19	17.58994601282829	32.41153426997301	49.695814317538066	0.30270539966063204
20-21	17.636470120454213	32.38557687873089	49.67914649933658	0.29880650147831544
22-23	17.660124874726314	32.345121206565054	49.696414768219825	0.29833915048881143
24-25	17.7155164187825	32.34243012556417	49.639708965906784	0.30234448974654815
26-27	17.761418684802205	32.37001446189585	49.56994884809093	0.29861800521101056
28-29	17.822345047469014	32.31873456418693	49.56216438856149	0.29675599978256667
30-31	17.82276347121952	32.293208664086805	49.58595546535932	0.29807239933436136
32-33	17.854863067184095	32.276986694014454	49.56513692842355	0.3030133103779015
34-35	17.880244102699468	32.259724116117795	49.56098487551116	0.29904690567158193
36-37	17.902405230452977	32.23501332603497	49.55761893489502	0.30496250861703783
38-39	17.947256851190204	32.224862755141004	49.52482712706367	0.303053266605118
40-41	17.941947163477913	32.22833255568746	49.5197210981046	0.3099991827300268
42-43	17.970343677467714	32.21196970863775	49.50264163096187	0.31504498293266636
44-45	17.980494563627506	32.187473534823305	49.52116284555457	0.310869055994625
46-47	18.02687037659323	32.15964618882507	49.49822382216645	0.31525961241525224
48-49	18.043868125608167	32.152232291521756	49.47490147422182	0.328998108648261
50-51	18.055984939531726	32.16329656981832	49.453484508110535	0.32723398253941444
52-53	18.065199497355774	32.14718720391826	49.45312711807707	0.3344861806489011
54-55	18.099576912512997	32.10593558944987	49.4430019366563	0.351485561380834
56-57	18.138367369610613	32.101956366432574	49.40782038941882	0.3518558745379933
58-59	18.154066737785634	32.09423117171359	49.38705468830247	0.3646474021983028
60-61	18.151768180371864	32.078370877856976	49.39074061356112	0.37912032821004127
62-63	18.162416158291105	32.06967181257777	49.37752702825027	0.39038500088085387
64-65	18.201094401525218	32.053174639454596	49.34215412951617	0.40357682950402285
66-67	18.24906001784096	32.016938463802056	49.30977503196208	0.42422648639490407
68-69	18.268642776662524	32.02854652664593	49.25946613326087	0.44334456343066875
70-71	18.267239613001617	32.02010269693476	49.249858941221135	0.4627987488424844
72-73	18.27053971438528	32.00930901630015	49.239499973794295	0.4806512955202684
74-75	18.31259439559241	32.012692303384945	49.168376357269935	0.5063369437527041
76-77	18.167799766990875	32.01716896844899	49.285800314323026	0.5292309502371146
78-79	18.222915418288757	31.987628041098077	49.238944104963856	0.5505124356493146
80-81	18.23764851027037	31.96764277030106	49.21953599842743	0.5751727210011331
82-83	18.22243570334885	31.974739420680866	49.19940827417481	0.603416601795475
84-85	18.213448238900153	31.964643162053036	49.18875345607894	0.63315514296787
86-87	18.25652814368633	31.927408987314227	49.1539865259985	0.6620763430009413
88-89	18.238982576342952	31.88713404395281	49.181759315999216	0.6921240637050274
90	18.235025665443146	31.874607417340865	49.17418789634044	0.7161790208755442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19406.0
1	18753.0
2	23832.0
3	42419.0
4	80773.0
5	159257.0
6	309399.5
7	568270.0
8	977638.0
9	1562176.0
10	1899059.0
11	2308777.0
12	3162329.0
13	4030077.0
14	4793308.5
15	5353215.0
16	5645919.5
17	5652058.5
18	5394355.0
19	4934134.5
20	4665641.0
21	4354226.0
22	3726857.5
23	3112669.0
24	2547823.0
25	2051142.0
26	1632306.5
27	1290065.5
28	1013154.0
29	794236.0
30	698754.0
31	624946.5
32	492242.5
33	385593.0
34	299012.5
35	232373.0
36	183834.5
37	145217.5
38	113181.0
39	86718.0
40	74401.0
41	64677.0
42	47226.5
43	33932.5
44	24467.0
45	17728.0
46	12733.0
47	9278.5
48	7361.0
49	9788.5
50	12833.0
51	9710.5
52	5298.5
53	3269.0
54	2136.0
55	1504.0
56	1054.0
57	773.5
58	602.0
59	412.5
60	325.0
61	293.5
62	228.5
63	157.5
64	109.0
65	93.0
66	71.0
67	48.5
68	37.5
69	34.5
70	37.0
71	30.0
72	21.5
73	16.5
74	9.5
75	8.5
76	9.0
77	5.5
78	7.5
79	7.0
80	3.0
81	4.5
82	6.0
83	5.5
84	4.0
85	4.5
86	5.5
87	3.0
88	2.0
89	2.5
90	2.0
91	1.5
92	3.0
93	3.5
94	5.5
95	11.0
96	12.5
97	15.0
98	22.0
99	42.0
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06241469084140108
2	0.0
3	0.0
4	0.28477891164547403
5	0.0
6	0.0
7	1.4627300408108995E-6
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.925460081621799E-6
22-23	0.0
24-25	0.015366710443738904
26-27	0.01133469508624366
28-29	0.04641900648011349
30-31	0.06459708406229095
32-33	0.09485804314658683
34-35	0.09898001640159194
36-37	0.13265937559126292
38-39	0.14881522889201929
40-41	0.1752533430146559
42-43	0.20428634022969105
44-45	0.23766876522107738
46-47	0.2659638151305234
48-49	0.26902530910594064
50-51	0.27715808813284926
52-53	0.24162544998147087
54-55	0.2735027257608628
56-57	0.30072120636027205
58-59	0.2743145409335129
60-61	0.2582332868648378
62-63	0.26544308323599475
64-65	0.24539856212174257
66-67	0.27162384902344117
68-69	0.2517065854227396
70-71	0.2426793469758751
72-73	0.2824941273217274
74-75	0.2785849812876603
76-77	0.2419516387805717
78-79	0.24034482983074096
80-81	0.22802644879205192
82-83	0.2119035069172138
84-85	0.23264136207081032
86-87	0.20890710442861266
88-89	0.27374700167767824
90	0.27818492462149846
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
90	6.8365315E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.07684453425361
#Duplication Level	Percentage of total
1	85.31589708815402
2	6.8989150416242815
3	1.7122409926893567
4	0.9458256056653698
5	0.6921103709541111
6	0.5282334485891276
7	0.41025745924953716
8	0.3272416664985387
9	0.2722312806360145
>10	2.410950395818163
>50	0.2485876963999328
>100	0.17613252074176455
>500	0.007789754236140894
>1k	0.03391126217438276
>5k	0.0
>10k+	0.019675416569531052
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.313650204054497E-6	0.0	0.0	0.0	0.007259529192544494	9.259081158332994E-4
2	1.4627300408108995E-5	0.0	0.0	0.0	0.010060657220697367	0.0014071462992600854
3	1.7552760489730794E-5	0.0	0.0	0.0	0.012848620678482942	0.001831338011095246
4	2.3403680652974392E-5	0.0	0.0	0.0	0.015548820333819862	0.0023052625443179776
5	2.6329140734596192E-5	0.0	0.0	0.0	0.01798572858181082	0.002864025419907741
6	2.779187077540709E-5	0.0	0.0	0.0	0.020500161521964756	0.0035134775580277808
7	3.218006089783979E-5	0.0	0.0	0.0	0.023080417313955184	0.004307739970188099
8	3.9493711101894284E-5	0.0	0.0	0.0	0.025656284915823176	0.005195617104960315
9	4.095644114270519E-5	0.0	0.0	0.0	0.02844132291352713	0.006298515555731733
10-11	4.7538726326354236E-5	0.0	0.0	0.0	0.03260864079979738	0.008238095589846986
12-13	6.728558187730138E-5	0.0	0.0	7.313650204054497E-7	0.03822844961659286	0.01174937905281355
14-15	2.530522970602856E-4	0.0	0.0	1.4627300408108995E-6	0.04340358850098182	0.01618657063161341
16-17	4.4759539248813527E-4	0.0	0.0	1.4627300408108995E-6	0.048308122327820766	0.021028938431717898
18-19	5.097614192225985E-4	0.0	0.0	1.4627300408108995E-6	0.05321631297976174	0.02578281106435332
20-21	0.008917533693803648	0.0	0.0	1.4627300408108995E-6	0.05802064979880514	0.030628835689559833
22-23	0.009193258306496503	0.0	0.0	1.4627300408108995E-6	0.06274599919564475	0.035482173964970394
24-25	0.009374636831557055	0.0	0.0	1.4627300408108995E-6	0.06730606009787272	0.04035233363585028
26-27	0.009734468421596537	0.0	0.0	1.4627300408108995E-6	0.07191804791654949	0.04538266224619897
28-29	0.01065160015718497	0.0	0.0	1.4627300408108995E-6	0.07644592875787964	0.050520501514547254
30-31	0.012504147753871975	0.0	0.0	1.4627300408108995E-6	0.0811120375880664	0.055712461794405546
32-33	0.015908651923859343	0.0	0.0	1.4627300408108995E-6	0.08584543200013048	0.0608473756026722
34-35	0.021388038656736972	0.0	0.0	1.4627300408108995E-6	0.09047058438917455	0.06591427246404116
36-37	0.029620283326420714	0.0	0.0	1.4627300408108995E-6	0.09514181277450415	0.07114572645500133
38-39	0.04120071705952061	0.0	0.0	1.4627300408108995E-6	0.0997121127870178	0.07645616786816531
40-41	0.056499410556361804	0.0	0.0	1.4627300408108995E-6	0.10437602752214335	0.08181634210271685
42-43	0.07602173704604448	0.0	0.0	1.4627300408108995E-6	0.10903774816220768	0.087284026995268
44-45	0.10023942696673013	0.0	0.0	1.4627300408108995E-6	0.11363876550557839	0.09276853328328846
46-47	0.12988238260878343	0.0	0.0	1.4627300408108995E-6	0.11836777172752003	0.09833641518363515
48-49	0.16502227774420405	0.0	0.0	1.4627300408108995E-6	0.1230887329342372	0.10401107637696104
50-51	0.20580611674209356	0.0	0.0	1.4627300408108995E-6	0.12786015832736236	0.10974205267685813
52-53	0.2522053763666561	0.0	0.0	1.4627300408108995E-6	0.13254528264807966	0.11545328212120429
54-55	0.30451918491123753	0.0	0.0	1.4627300408108995E-6	0.13733206670663334	0.12122814032232573
56-57	0.36243890633722675	0.0	0.0	1.4627300408108995E-6	0.14208886479935037	0.12728164859622165
58-59	0.42681950635347765	0.0	0.0	1.4627300408108995E-6	0.14672059947357807	0.1332737222084035
60-61	0.4966985085931367	0.0	0.0	1.4627300408108995E-6	0.15137134963833634	0.13930236407160562
62-63	0.5725118066083656	0.0	0.0	1.4627300408108995E-6	0.1560762208146046	0.14539097786648097
64-65	0.6534095542454532	0.0	0.0	1.4627300408108995E-6	0.16084910893777055	0.15144082931527486
66-67	0.7395387558735012	0.0	0.0	1.4627300408108995E-6	0.16553277052844706	0.15699774074031547
68-69	0.8306397769102651	0.0	0.0	1.4627300408108995E-6	0.17030273319153139	0.16273749342045743
70-71	0.9258847121526463	0.0	0.0	1.4627300408108995E-6	0.17517947514759497	0.16848529111582386
72-73	1.025154349102319	0.0	0.0	1.4627300408108995E-6	0.1800569484686789	0.17414825046882326
74-75	1.1278277588569585	0.0	0.0	1.4627300408108995E-6	0.18496001956547703	0.17974684970002697
76-77	1.2335926485528517	0.0	0.0	1.4627300408108995E-6	0.1899910795408461	0.18522038551274136
78-79	1.3415399314696348	0.0	0.0	1.4627300408108995E-6	0.19501994542115397	0.19080070061843496
>>END_MODULE
