##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR546471_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70799691
Total Bases	6.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90
%GC	19
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.27116967219532	38.0	38.0	38.0	36.0	38.0
2	37.24951600424358	38.0	38.0	38.0	36.0	38.0
3	37.23033518606741	38.0	38.0	38.0	36.0	38.0
4	37.226089065840696	38.0	38.0	38.0	36.0	38.0
5	37.23042813845049	38.0	38.0	38.0	36.0	38.0
6	37.24353909962686	38.0	38.0	38.0	36.0	38.0
7	37.19448241659699	38.0	38.0	38.0	36.0	38.0
8	37.17453939170441	38.0	38.0	38.0	36.0	38.0
9	37.16753405039579	38.0	38.0	38.0	36.0	38.0
10-11	37.11757562614221	38.0	38.0	38.0	36.0	38.0
12-13	37.11018717581691	38.0	38.0	38.0	36.0	38.0
14-15	37.07497579332656	38.0	38.0	38.0	36.0	38.0
16-17	37.0395132021127	38.0	38.0	38.0	36.0	38.0
18-19	36.99094986869363	38.0	38.0	38.0	36.0	38.0
20-21	36.933984796628565	38.0	38.0	38.0	35.0	38.0
22-23	36.84249325325445	38.0	37.5	38.0	35.0	38.0
24-25	36.78736508186172	38.0	37.0	38.0	35.0	38.0
26-27	36.54835615031145	38.0	37.0	38.0	35.0	38.0
28-29	36.67661953496379	38.0	37.0	38.0	35.0	38.0
30-31	36.667732003237134	38.0	37.0	38.0	35.0	38.0
32-33	36.644463349423376	38.0	37.0	38.0	35.0	38.0
34-35	36.58610904106912	38.0	37.0	38.0	35.0	38.0
36-37	36.52153738919567	38.0	37.0	38.0	35.0	38.0
38-39	36.554019134066564	38.0	37.0	38.0	35.0	38.0
40-41	36.52048490578864	38.0	37.0	38.0	35.0	38.0
42-43	36.455262530736185	38.0	37.0	38.0	35.0	38.0
44-45	36.39223308192122	38.0	37.0	38.0	34.5	38.0
46-47	36.03695256240596	38.0	36.0	38.0	33.5	38.0
48-49	35.962048337188364	38.0	36.0	38.0	33.0	38.0
50-51	35.81556108062675	38.0	36.0	38.0	32.5	38.0
52-53	35.69467744569676	37.0	36.0	38.0	32.0	38.0
54-55	35.67537896457768	37.0	36.0	38.0	32.0	38.0
56-57	36.150130634044714	38.0	37.0	38.0	34.0	38.0
58-59	36.087001933384144	38.0	36.5	38.0	34.0	38.0
60-61	36.0592028925663	38.0	36.5	38.0	34.0	38.0
62-63	35.9847428359539	38.0	36.0	38.0	33.0	38.0
64-65	35.92286522126206	38.0	36.0	38.0	33.0	38.0
66-67	35.83403628131654	38.0	36.0	38.0	33.0	38.0
68-69	35.76133301909467	38.0	36.0	38.0	33.0	38.0
70-71	35.69255515536078	38.0	36.0	38.0	33.0	38.0
72-73	35.602435891987156	38.0	36.0	38.0	32.0	38.0
74-75	35.53524025267286	38.0	36.0	38.0	32.0	38.0
76-77	35.47328445261152	38.0	36.0	38.0	32.0	38.0
78-79	35.36270954911371	38.0	36.0	38.0	32.0	38.0
80-81	35.265614012354945	38.0	36.0	38.0	32.0	38.0
82-83	35.11499680132785	37.0	35.5	38.0	32.0	38.0
84-85	34.965579235367	37.0	35.0	38.0	31.5	38.0
86-87	34.78409809726429	37.0	35.0	38.0	31.0	38.0
88-89	34.58007837209346	37.0	35.0	38.0	30.0	38.0
90	34.39824661099156	37.0	35.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	274631.0
3	17280.0
4	14894.0
5	21111.0
6	23686.0
7	33320.0
8	31140.0
9	47057.0
10	43450.0
11	63623.0
12	60913.0
13	78294.0
14	67081.0
15	75674.0
16	63266.0
17	76513.0
18	68791.0
19	87467.0
20	87700.0
21	115292.0
22	130080.0
23	165502.0
24	195282.0
25	243630.0
26	281243.0
27	342290.0
28	397774.0
29	483849.0
30	582960.0
31	734086.0
32	936069.0
33	1275355.0
34	1853381.0
35	3171019.0
36	8081642.0
37	5.0572041E7
38	2305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.4363221318729185	47.316560603671704	31.78134407480665	20.46577318964873
2	0.43877266467413845	51.798624120841716	31.14855562727063	16.614047587213513
3	0.3892969969105278	48.73110292797359	32.47631709428314	18.403282980832735
4	0.3789774606965619	48.916375997622694	31.98444792730259	18.720198614378155
5	0.37068311902331	49.418904419100755	32.02441541068351	18.18599705119242
6	0.36488736976052377	49.60884835822812	31.211735761047883	18.81452851096347
7	0.3679774844842339	49.74462101870584	31.689883233307825	18.1975182635021
8	0.34110191869122286	49.08158265973811	32.000691988389185	18.57662343318148
9	0.34542911881040417	48.666390458351074	32.3019116470556	18.68626877578292
10-11	0.34930025491971933	48.977509406653056	32.084341743528434	18.588848594898792
12-13	0.34015354114413493	49.01787564087251	32.08734683864172	18.554623979341628
14-15	0.34627774909756226	48.85986641354228	32.20957283189192	18.58428300546824
16-17	0.3468977268784482	48.80872363471604	32.106638982131955	18.73773965627356
18-19	0.355684269825095	48.69972901921155	32.01089559786999	18.933691113093367
20-21	0.354012772010777	48.724920918973844	31.923668370785073	18.997397938230307
22-23	0.35199504825219297	48.719282006176215	31.919796374829396	19.00892657074219
24-25	0.357673537793028	48.755021104510746	31.83781490149327	19.049490456202957
26-27	0.37488967345051005	48.81170465281128	31.67292445941254	19.14048121432568
28-29	0.3582470268312886	48.79681801986679	31.723861273793556	19.121073679508363
30-31	0.36026094941292375	48.77241814311273	31.676284383292476	19.19103652418187
32-33	0.3631986539916587	48.800355485181655	31.691919746713843	19.14452611411285
34-35	0.3632221953844087	48.81792388630235	31.67143047279935	19.147423445513887
36-37	0.3723150545513455	48.81806701662504	31.628273260551477	19.181344668272146
38-39	0.3787175381320798	48.812278566124746	31.610094743119095	19.198909152624083
40-41	0.3895177183729134	48.79086189742791	31.59110295773777	19.228517426461405
42-43	0.4078741337961333	48.73734312830976	31.593754190733353	19.26102854716075
44-45	0.4160882830521304	48.78079667563283	31.583213586943298	19.219901454371744
46-47	0.43974371104409365	48.79180163986071	31.541651615689446	19.226803033405755
48-49	0.4644240826675037	48.76217620654536	31.51908307027995	19.25431664050718
50-51	0.4902880638758022	48.776545890360666	31.51471528409201	19.21845076167152
52-53	0.5175621518401996	48.73415302185294	31.528283364861103	19.22000146144576
54-55	0.5519183238996219	48.70365947077932	31.5052303012073	19.239191904113763
56-57	0.6001229586276685	48.71617760306427	31.456953279574062	19.226746158734002
58-59	0.6486402600835278	48.6961905282941	31.437099849123673	19.218069362498703
60-61	0.7081474804684849	48.62788137912627	31.418848081681766	19.245123058723472
62-63	0.7612254717182818	48.606941356226294	31.405469904832632	19.22636326722279
64-65	0.8228907970075142	48.59353122594927	31.371536858307113	19.2120411187361
66-67	0.885650634632283	48.56767319435516	31.339855417558464	19.206820753454092
68-69	0.9481003911363535	48.55295918150409	31.31450264006861	19.184437787290943
70-71	1.0217389172635882	48.49872337554609	31.298581972848368	19.180955734341953
72-73	1.0913135712147062	48.454586760749905	31.270080753379116	19.184018914656274
74-75	1.1646261416606234	48.435553754663644	31.24228640159744	19.157533702078297
76-77	1.2418383790647145	48.392193655670624	31.223632049014032	19.142335916250627
78-79	1.322290486169997	48.34440753761192	31.17562119988796	19.15768077633012
80-81	1.4017512029269767	48.31196570006557	31.164749819112085	19.121533277895363
82-83	1.4827166955978572	48.28182829830408	31.123753552026727	19.11170145407134
84-85	1.562757703042115	48.19244076799517	31.11719029799287	19.127611230969848
86-87	1.6430498153036555	48.17474483897288	31.109713784212257	19.07249156151121
88-89	1.7258358053752094	48.11455380132681	31.09214431026818	19.06746608302981
90	1.7839490630278159	48.04114701212392	31.0612298043549	19.11367412049336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16907.0
1	14201.5
2	12835.0
3	18141.5
4	31751.5
5	65849.0
6	143270.5
7	298560.5
8	571760.5
9	997812.0
10	1252987.0
11	1592950.5
12	2342106.0
13	3198847.0
14	4078138.5
15	4859857.5
16	5434375.5
17	5735394.5
18	5736108.5
19	5470739.0
20	5281133.0
21	5009895.5
22	4434721.5
23	3824771.5
24	3228507.0
25	2676974.0
26	2195004.5
27	1788759.5
28	1444131.5
29	1159434.0
30	1033807.0
31	932505.5
32	746596.5
33	592217.0
34	463508.5
35	360247.5
36	286637.5
37	228066.5
38	179101.0
39	140140.5
40	120860.0
41	104931.0
42	76772.5
43	55326.5
44	40482.5
45	30834.5
46	24375.0
47	20228.5
48	17899.0
49	23938.0
50	30599.0
51	21482.0
52	10700.5
53	8212.0
54	6634.0
55	5373.5
56	4563.0
57	3988.5
58	3484.5
59	3025.5
60	2800.0
61	2613.0
62	2296.5
63	2069.0
64	1878.0
65	1755.5
66	1674.5
67	1519.5
68	1419.5
69	1354.0
70	1285.0
71	1261.0
72	1238.0
73	1205.5
74	1186.5
75	1123.0
76	1047.0
77	1012.0
78	963.5
79	927.5
80	903.0
81	849.5
82	786.0
83	770.0
84	730.5
85	686.0
86	635.5
87	600.0
88	557.5
89	498.5
90	486.0
91	479.0
92	428.5
93	341.0
94	280.0
95	254.5
96	210.5
97	155.0
98	115.0
99	97.5
100	100.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15950069612591952
2	0.12834095561236278
3	0.12374630279106727
4	0.1382223546710112
5	0.11335925180803402
6	0.11037053819910032
7	0.09708093217525483
8	0.10729001627987332
9	0.1029383023719694
10-11	0.19773320762092025
12-13	0.09286975560387685
14-15	0.035313713445444275
16-17	0.020569298812335213
18-19	0.018014202915094642
20-21	0.04107433180746509
22-23	0.03828335917454781
24-25	0.08618964170337975
26-27	0.03550227359043135
28-29	0.06516695108174977
30-31	0.06494025517710239
32-33	0.029565100785538735
34-35	0.0759028736438977
36-37	0.04928270096546043
38-39	0.05290206704433216
40-41	0.03747262117288054
42-43	0.07882520278231157
44-45	0.13229224404383347
46-47	0.13688972173621494
48-49	0.0927023819920344
50-51	0.16245974858845075
52-53	0.08689303460378096
54-55	0.11860786228572663
56-57	0.09572005052959906
58-59	0.048188769637426805
60-61	0.08860349404632288
62-63	0.040223339392823056
64-65	0.10386839117701799
66-67	0.11265868377871874
68-69	0.07920232307228572
70-71	0.1804697141969165
72-73	0.08123199294753984
74-75	0.07697632465655818
76-77	0.052175368957471865
78-79	0.025858163703002604
80-81	0.07365921978388296
82-83	0.018846127449906526
84-85	0.04700938595904324
86-87	0.03513292169594356
88-89	0.016074928914590884
90	0.09322215827184896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
90	7.0799691E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.07836750242997
#Duplication Level	Percentage of total
1	82.70336680936587
2	7.825977751989169
3	1.8899202867318905
4	0.9451298291255756
5	0.7596421744906809
6	0.5698848566935756
7	0.4750733594683942
8	0.39717777084224815
9	0.36433736476493495
>10	3.2338292603321652
>50	0.43382999409597306
>100	0.3040339887764819
>500	0.021653184417694846
>1k	0.04105366775637156
>5k	0.020377174592115497
>10k+	0.01471252655703376
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.649742171897332E-6	0.0	0.0	0.0	0.19359689013331993	1.3983111875445897E-4
2	1.1299484343794665E-5	0.0	0.0	1.412435542974333E-6	0.2429375009560423	1.8502905612963763E-4
3	2.1186533144614997E-5	0.0	0.0	1.412435542974333E-6	0.2814984037147846	2.344643001337393E-4
4	2.6836275316512327E-5	0.0	0.0	1.412435542974333E-6	0.32086721960410813	2.8813685076676393E-4
5	3.107358194543533E-5	0.0	0.0	1.412435542974333E-6	0.3612953621506625	3.3474722368491693E-4
6	3.672332411733266E-5	0.0	0.0	1.412435542974333E-6	0.4008492070961157	3.968943875757876E-4
7	4.2373066289229994E-5	0.0	0.0	2.824871085948666E-6	0.43979287988700405	4.4350476049394055E-4
8	4.661037291815299E-5	0.0	0.0	2.824871085948666E-6	0.478093894505839	4.887026978691192E-4
9	5.084767954707599E-5	0.0	0.0	4.237306628922999E-6	0.516451406546393	5.466125551310669E-4
10-11	6.355959943384499E-5	0.0	0.0	4.237306628922999E-6	0.5740695958687164	6.348897765669627E-4
12-13	8.474613257845997E-5	0.0	0.0	4.237306628922999E-6	0.6501292498578843	7.577716688057297E-4
14-15	2.2669590464738045E-4	0.0	0.0	4.237306628922999E-6	0.7230158673997602	8.940716987027527E-4
16-17	3.757078544311726E-4	0.0	0.0	5.6497421718973315E-6	0.7952923127870714	0.0010459085195724937
18-19	4.166684851774282E-4	0.0	0.0	7.062177714871665E-6	0.8665906465608727	0.0011617282340963889
20-21	0.007475315111191658	0.0	0.0	7.062177714871665E-6	0.9377209005050602	0.0012761355130773099
22-23	0.0077189602423547304	0.0	0.0	7.062177714871665E-6	1.0086816621840906	0.0013587629923413084
24-25	0.007896220902998009	0.0	0.0	7.062177714871665E-6	1.0789192568651182	0.0014519837381776143
26-27	0.008363030849951027	0.0	0.0	7.768395486358832E-6	1.1497606677407675	0.0015226055153263309
28-29	0.009581256505766387	0.0	0.0	8.474613257845998E-6	1.2201493930248932	0.0015755718481878685
30-31	0.01213070266083506	0.0	0.0	8.474613257845998E-6	1.2898848668703935	0.001615826261162637
32-33	0.016892729093973022	0.0	0.0	8.474613257845998E-6	1.3591860167864294	0.0016617304163093027
34-35	0.024912538106981283	0.0	0.0	8.474613257845998E-6	1.427729395033659	0.0016991599581981226
36-37	0.037452847075278906	0.0	0.0	8.474613257845998E-6	1.496224750472428	0.001725996233514635
38-39	0.05554049663860822	0.0	0.0	8.474613257845998E-6	1.5640541425526844	0.0017485952022022245
40-41	0.07975034806295976	0.0	0.0	8.474613257845998E-6	1.6317557092163015	0.0017662506464894035
42-43	0.11094045594069048	0.0	0.0	8.474613257845998E-6	1.6992560038150448	0.0017944993573488902
44-45	0.14979726394568588	0.0	0.0	8.474613257845998E-6	1.7668657621683688	0.0018142734549505307
46-47	0.1985849062533338	0.0	0.0	8.474613257845998E-6	1.8337841333234066	0.00183687242363812
48-49	0.25639871789835916	0.0	0.0	8.474613257845998E-6	1.9008402169438847	0.0018580589567827352
50-51	0.3261659715435764	0.0	0.0	8.474613257845998E-6	1.9681364146066684	0.001875008183298427
52-53	0.405400074415579	0.0	0.0	8.474613257845998E-6	2.0353973013808773	0.001904669329700888
54-55	0.494880690934089	0.0	0.0	8.474613257845998E-6	2.1020084678053186	0.0019364491294178105
56-57	0.5953528808480252	0.0	0.0	9.180831029333164E-6	2.1687029679267953	0.001963285404734323
58-59	0.7064889591114176	0.0	0.0	9.88704880082033E-6	2.2348649798485702	0.001997183857765707
60-61	0.8273757861457334	0.0	0.0	9.88704880082033E-6	2.30051145279716	0.0020303760930256037
62-63	0.9573135001394286	0.0	0.0	1.1299484343794665E-5	2.366469367782975	0.002067805634914424
64-65	1.095006897699596	0.0	0.0	1.1299484343794665E-5	2.4319209246266342	0.0021059413945747306
66-67	1.2417469449125138	0.0	0.0	1.1299484343794665E-5	2.4971330736457595	0.0021476082430924737
68-69	1.39519606095456	0.0	0.0	1.1299484343794665E-5	2.561958215326109	0.0021765631717234474
70-71	1.5566911160671593	0.0	0.0	1.1299484343794665E-5	2.6266717745985644	0.002216817584698216
72-73	1.724866991298027	0.0	0.0	1.1299484343794665E-5	2.691429825590623	0.0022676652642452918
74-75	1.8986876651763918	0.0	0.0	1.1299484343794665E-5	2.7558001630261355	0.0023065072416770856
76-77	2.07797093351721	0.0	0.0	1.2005702115281831E-5	2.8196041138089147	0.0023375808236225214
78-79	2.2636362918589574	0.0	0.0	1.2711919886768997E-5	2.8848819410807884	0.0023757165832828282
>>END_MODULE
