##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR546471_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70799691
Total Bases	6.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90
%GC	19
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.267705349166	38.0	37.0	38.0	36.0	38.0
2	37.19805613275911	38.0	37.0	38.0	36.0	38.0
3	37.18356735483493	38.0	37.0	38.0	36.0	38.0
4	37.126145720042764	38.0	37.0	38.0	36.0	38.0
5	37.1581553936443	38.0	37.0	38.0	36.0	38.0
6	37.20026834580394	38.0	37.0	38.0	36.0	38.0
7	37.16310068358914	38.0	37.0	38.0	36.0	38.0
8	37.13240406656577	38.0	37.0	38.0	36.0	38.0
9	37.11490163142096	38.0	37.0	38.0	36.0	38.0
10-11	37.077413049726445	38.0	37.0	38.0	35.5	38.0
12-13	37.00743823161601	38.0	37.0	38.0	35.0	38.0
14-15	36.977190882090156	38.0	37.0	38.0	35.0	38.0
16-17	36.87847446255097	38.0	37.0	38.0	35.0	38.0
18-19	36.81794830855971	38.0	37.0	38.0	35.0	38.0
20-21	36.73641275496527	38.0	36.5	38.0	35.0	38.0
22-23	36.61669319997456	38.0	36.0	38.0	34.5	38.0
24-25	36.62136154662031	38.0	36.0	38.0	34.5	38.0
26-27	36.70705797289426	38.0	37.0	38.0	35.0	38.0
28-29	36.68031573047402	38.0	36.0	38.0	35.0	38.0
30-31	36.66307291510637	38.0	36.5	38.0	35.0	38.0
32-33	36.642623122465324	38.0	36.5	38.0	35.0	38.0
34-35	36.58062065553365	38.0	36.0	38.0	35.0	38.0
36-37	36.459497775209215	38.0	36.0	38.0	34.0	38.0
38-39	36.37761465653854	38.0	36.0	38.0	34.0	38.0
40-41	36.286597105346125	38.0	36.0	38.0	34.0	38.0
42-43	36.17729325259343	38.0	36.0	38.0	33.5	38.0
44-45	36.097255438586586	37.0	36.0	38.0	33.0	38.0
46-47	35.98135979152791	37.0	36.0	38.0	33.0	38.0
48-49	35.883772020982406	37.0	36.0	38.0	33.0	38.0
50-51	35.74537764578662	37.0	36.0	38.0	33.0	38.0
52-53	35.591675378922204	37.0	36.0	38.0	32.0	38.0
54-55	35.45858692377627	37.0	35.5	38.0	32.0	38.0
56-57	35.24972718454379	37.0	35.0	38.0	32.0	38.0
58-59	35.095850545449416	37.0	35.0	38.0	31.5	38.0
60-61	34.88020866503499	37.0	35.0	38.0	31.0	38.0
62-63	34.69372463080383	36.5	35.0	38.0	30.0	38.0
64-65	34.5431214381995	36.0	34.0	38.0	30.0	38.0
66-67	34.25328686815879	36.0	34.0	38.0	30.0	38.0
68-69	34.058819430440735	36.0	34.0	38.0	29.0	38.0
70-71	33.49268862628229	36.0	33.5	37.0	28.0	38.0
72-73	32.891111276177746	35.0	32.0	37.0	27.0	38.0
74-75	32.67531429621635	35.0	32.0	37.0	26.0	38.0
76-77	33.05575348485631	36.0	33.0	37.0	27.0	38.0
78-79	32.90788133524481	36.0	33.0	37.0	26.5	38.0
80-81	33.120192996322544	36.0	33.5	37.5	27.0	38.0
82-83	33.27589693152757	36.0	33.5	38.0	26.5	38.0
84-85	32.95514791865405	36.0	33.0	37.0	25.5	38.0
86-87	32.22246933252858	36.0	32.5	37.0	23.5	38.0
88-89	31.831148133118262	36.0	32.0	37.0	20.5	38.0
90	31.498220324153674	35.0	32.0	37.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49559.0
3	5459.0
4	2853.0
5	5394.0
6	9650.0
7	14592.0
8	21667.0
9	27192.0
10	33074.0
11	46237.0
12	50135.0
13	70610.0
14	76421.0
15	98737.0
16	100736.0
17	125642.0
18	135333.0
19	163682.0
20	176239.0
21	206978.0
22	223913.0
23	265876.0
24	301122.0
25	364873.0
26	428008.0
27	524094.0
28	634992.0
29	787784.0
30	986838.0
31	1276734.0
32	1689717.0
33	2367497.0
34	3737496.0
35	7152621.0
36	2.2597365E7
37	2.6040565E7
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.25611438561531	32.736751253958666	49.14962958183126	0.8575047785947545
2	18.938688159635102	34.45084681590119	46.14945303329279	0.4610119911709219
3	17.88963281891003	32.57600353221986	49.08500279732942	0.4493608515406923
4	18.437150416028707	32.61890809548527	48.512344044225735	0.43159744426028973
5	18.573189616579157	32.61733108345985	48.412837047571436	0.39664225238956075
6	18.653797514455253	32.69729524666993	48.24554672138329	0.40336051749152413
7	18.977496667323027	32.55871272093546	48.099516423030714	0.36427418871079537
8	18.22518829919752	32.489987844720964	48.91841406482975	0.3664097912517726
9	18.48636881762662	31.843162987815866	49.294754690384174	0.3757135041733445
10-11	18.45554029324789	32.30490864712955	48.886459829323265	0.35309123029929607
12-13	18.23574837353457	32.585455069288365	48.83094970004883	0.3478468571282324
14-15	18.319554068975673	32.331884231523055	49.00133774109334	0.34722395840793047
16-17	18.465965494075714	32.173213254849976	49.00884629504363	0.351974956030673
18-19	18.58642010174875	32.018204712221134	49.033038152666514	0.36233703336360606
20-21	18.638546035462216	31.974157909813478	49.0349407033429	0.35235535138140645
22-23	18.679707938273346	31.942890824198656	49.02722103688277	0.350180200645226
24-25	18.76384531548469	31.927588133082725	48.9523544723948	0.3562120790377866
26-27	18.850365309268287	31.913757398024075	48.8806941703435	0.35518312236413263
28-29	18.902940758928665	31.869600571970036	48.868059304306236	0.3593993647950658
30-31	18.91531690989677	31.8442578091369	48.877704967030574	0.36272031393575654
32-33	18.95301091414189	31.83909654531859	48.841485160137395	0.36640738040213244
34-35	18.98778555403582	31.832326782330167	48.8133104987704	0.366577164863615
36-37	19.018443191842774	31.781939297031776	48.82429993211781	0.37531757900764007
38-39	19.083289771710156	31.743180684015503	48.7990455713232	0.3744839729511388
40-41	19.090883530462396	31.73236684207405	48.79234299259059	0.3844066348729613
42-43	19.097447675295832	31.709451384628274	48.806895711207474	0.38620522886842706
44-45	19.134631969382575	31.72230848655633	48.75258467202214	0.39047487203896175
46-47	19.178587883215865	31.691639128801295	48.730031455637615	0.39974153234522464
48-49	19.226902915314856	31.664275340330793	48.69320833021603	0.41561341413831854
50-51	19.262199748434554	31.653793117208313	48.66316433698646	0.42084279737066965
52-53	19.275118483209816	31.650614876600606	48.6409986480764	0.4332679921131751
54-55	19.311133052391767	31.60947758467411	48.622443669045175	0.4569456938889496
56-57	19.351663473647065	31.606559018499198	48.573919404964485	0.46785810288924856
58-59	19.396510379756783	31.590460433285923	48.526579074339956	0.48645011261734306
60-61	19.411990374688223	31.562336015313065	48.513499551600916	0.5121740583978018
62-63	19.45651501050019	31.55131145266985	48.457610634237966	0.5345629025919861
64-65	19.506849916762658	31.545283188137418	48.38861309557492	0.5592537995250024
66-67	19.55826957996899	31.513262130156704	48.33552497007241	0.5929433198018916
68-69	19.58851809533165	31.511809106152544	48.2735392708122	0.6261335277036068
70-71	19.62673816676311	31.48586765771591	48.22815306544017	0.6592411100808099
72-73	19.658617226999954	31.46668770617716	48.18304752695183	0.6916475398710472
74-75	19.745566315852283	31.451418714794734	48.06879562009254	0.734219349260446
76-77	19.578672198123595	31.467134243484633	48.18393026843172	0.7702632899600447
78-79	19.643469341767826	31.438618282544578	48.10614867991971	0.8117636957678783
80-81	19.717187397962878	31.43012488050839	47.97135473704726	0.8813329844814705
82-83	19.702441128949058	31.417314230589678	47.97474635629526	0.9054982841660042
84-85	19.715923837084148	31.39578213703194	47.93165216774666	0.9566418581372443
86-87	19.750859571573997	31.37005520945493	47.87247721014428	1.0066080088267948
88-89	19.784744260071186	31.343123358197133	47.81453799654626	1.0575943851854248
90	19.766821380363158	31.308479451929237	47.82476863384804	1.0999305338595666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6237.0
1	6368.0
2	8142.5
3	13740.5
4	27851.5
5	62831.5
6	141302.5
7	299528.5
8	584278.5
9	1033550.0
10	1302650.0
11	1659669.0
12	2441191.5
13	3314557.5
14	4179230.0
15	4924356.0
16	5461642.0
17	5728519.5
18	5704778.5
19	5424507.0
20	5226884.0
21	4957195.0
22	4387885.0
23	3780317.0
24	3183411.5
25	2634515.5
26	2149537.5
27	1741554.5
28	1403219.0
29	1126083.5
30	1004341.0
31	907704.5
32	730229.5
33	583051.5
34	462110.0
35	365952.0
36	293417.5
37	236029.0
38	188503.5
39	147693.0
40	127998.0
41	112533.0
42	84214.0
43	61976.5
44	46279.5
45	34564.0
46	25804.0
47	19828.0
48	16030.0
49	22436.5
50	30023.0
51	23116.0
52	13464.0
53	9119.0
54	6563.5
55	5101.5
56	4134.0
57	3373.5
58	2694.0
59	2118.5
60	1923.0
61	1746.5
62	1391.5
63	1098.0
64	864.0
65	675.0
66	552.0
67	448.0
68	340.5
69	273.0
70	262.0
71	239.5
72	203.0
73	161.0
74	119.0
75	97.5
76	72.5
77	50.5
78	42.5
79	34.5
80	30.0
81	23.0
82	17.0
83	18.0
84	13.0
85	11.0
86	10.0
87	7.0
88	8.0
89	10.5
90	13.0
91	12.5
92	10.5
93	13.5
94	16.0
95	16.0
96	20.5
97	28.0
98	43.5
99	55.0
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011980278275508294
2	0.007679412047151449
3	2.9661146402460992E-5
4	0.1507534828082795
5	0.002310744548306009
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0038601863389488523
16-17	7.274043046317815E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0057697991930501505
26-27	4.187871384918897E-4
28-29	2.514135266494313E-4
30-31	3.354534414564041E-4
32-33	1.1299484343794664E-4
34-35	0.0
36-37	0.024719740655365287
38-39	0.02375999070391423
40-41	0.057212820321489825
42-43	0.037160472917883214
44-45	0.01754456809705568
46-47	0.015465462977797459
48-49	0.007480964853363555
50-51	0.0424225015332341
52-53	0.005509204835371386
54-55	0.05166336107314366
56-57	0.07142545297266904
58-59	0.05202494457214509
60-61	0.12841369604282593
62-63	0.03973463669495394
64-65	0.05732087164052736
66-67	0.0690892845845895
68-69	0.04085328564498961
70-71	0.07845231979896636
72-73	0.008336194574634513
74-75	0.012413895987201414
76-77	0.03713787394919563
78-79	0.030308041881143236
80-81	0.032137145909295
82-83	0.03088290314713379
84-85	0.07256458223807785
86-87	0.09062468930831916
88-89	0.06377852694300601
90	0.14194835963337749
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
90	7.0799691E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.64139172238329
#Duplication Level	Percentage of total
1	82.02773601816952
2	8.15506031403049
3	2.032714746483083
4	1.075081944473578
5	0.7225665343699555
6	0.5628393211487527
7	0.4449779403907257
8	0.41219683898446097
9	0.34825676447720044
>10	3.3236356645942524
>50	0.5055667953810148
>100	0.28894345226590484
>500	0.022964349580527708
>1k	0.04489013771649443
>5k	0.03256917793404211
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.954819520328132E-5	0.0	0.0	0.0	0.09751313745140498	0.0010014167999688021
2	4.661037291815299E-5	0.0	0.0	0.0	0.10309367028169657	0.0014985941110957674
3	5.932229280492199E-5	0.0	0.0	0.0	0.10588040560798492	0.00200142116439463
4	6.497203497681932E-5	0.0	0.0	0.0	0.10838889113230735	0.0025282596219240562
5	6.920934160574232E-5	0.0	0.0	0.0	0.1115061363756517	0.0031991665048368644
6	7.627151932061398E-5	0.0	0.0	0.0	0.1139877856246576	0.003920921067296749
7	8.192126149251131E-5	0.0	0.0	0.0	0.11651604524658166	0.004745783424393759
8	8.898343920738299E-5	0.0	0.0	0.0	0.11905277948176356	0.005728838562303894
9	9.322074583630598E-5	0.0	0.0	0.0	0.12170109612484044	0.006813589059308183
10-11	1.0734510126604931E-4	0.0	0.0	0.0	0.12566792134728383	0.008772637157413582
12-13	1.2782541663917713E-4	0.0	0.0	0.0	0.13077316961736457	0.012293132748277109
14-15	2.6906897093661043E-4	0.0	0.0	0.0	0.13545680587786746	0.016422388058162568
16-17	4.1808092072040257E-4	0.0	0.0	0.0	0.13983323740777343	0.020610259443081466
18-19	4.597477692381454E-4	0.0	0.0	0.0	0.1440401766725225	0.024748695583996262
20-21	0.007940006404830214	0.0	0.0	0.0	0.14825770920384385	0.02887653845833875
22-23	0.00819989454473749	0.0	0.0	0.0	0.15239967643361607	0.03307924041645888
24-25	0.008384217383095641	0.0	0.0	1.412435542974333E-6	0.15632059750091282	0.03738646260475911
26-27	0.008866564121021376	0.0	0.0	1.412435542974333E-6	0.16029307246552815	0.04186670814707369
28-29	0.010102445221123916	0.0	0.0	1.412435542974333E-6	0.16428249665665914	0.04641333815990807
30-31	0.012649772722878127	0.0	0.0	1.412435542974333E-6	0.1683771472957417	0.05101999668331886
32-33	0.01741462402710204	0.0	0.0	1.412435542974333E-6	0.17234538495372811	0.05569374589502092
34-35	0.025532597310347016	0.0	0.0	1.412435542974333E-6	0.17625783140776702	0.06036325780009407
36-37	0.03812869748259212	0.0	0.0	1.412435542974333E-6	0.18018369599946418	0.06510127282900147
38-39	0.05627072581432594	0.0	0.0	1.412435542974333E-6	0.18417382640836666	0.06972488057892795
40-41	0.08059427829988693	0.0	0.0	1.412435542974333E-6	0.18818161226155633	0.0744544209945775
42-43	0.11226743913331486	0.0	0.0	2.824871085948666E-6	0.1922896810382972	0.07934992258652654
44-45	0.15199854473941138	0.0	0.0	2.824871085948666E-6	0.1963122974646881	0.08431251486676686
46-47	0.2014472068811713	0.0	0.0	2.824871085948666E-6	0.20035398177090913	0.08931324290666749
48-49	0.2610809417233191	0.0	0.0	2.824871085948666E-6	0.20437659819730003	0.09440012951468954
50-51	0.33158548672196886	0.0	0.0	2.824871085948666E-6	0.20849243536952725	0.09945099901636575
52-53	0.4119177582286341	0.0	0.0	2.824871085948666E-6	0.21262380933272718	0.10462475041028074
54-55	0.5023277573344211	0.0	0.0	2.824871085948666E-6	0.21679614392667335	0.1097059872761309
56-57	0.6028755125499065	0.0	0.0	2.824871085948666E-6	0.2208385344506659	0.11497366563365369
58-59	0.714034895999758	0.0	0.0	2.824871085948666E-6	0.22485055761048447	0.12031549685718261
60-61	0.8350029380777948	0.0	0.0	2.824871085948666E-6	0.2288279760995002	0.12563119802316652
62-63	0.9648389567123958	0.0	0.0	2.824871085948666E-6	0.2328181065084027	0.13090240746954673
64-65	1.1022470422928823	0.0	0.0	2.824871085948666E-6	0.236654281443121	0.13652107605949862
66-67	1.2480965488959548	0.0	0.0	2.824871085948666E-6	0.24058014603481814	0.14177392384382018
68-69	1.4005385983958603	0.0	0.0	2.824871085948666E-6	0.24447069973794094	0.14700205400613967
70-71	1.5605901443835397	0.0	0.0	2.824871085948666E-6	0.24849896590650372	0.1521616810446249
72-73	1.7265936937493132	0.0	0.0	2.824871085948666E-6	0.25251522637295126	0.1573403759629403
74-75	1.8979496676051877	0.0	0.0	2.824871085948666E-6	0.25656326663911566	0.16239972007787434
76-77	2.074079673596316	0.0	0.0	2.824871085948666E-6	0.26061836908299496	0.16747530720155263
78-79	2.25608682388176	0.0	0.0	2.824871085948666E-6	0.2646734715268743	0.17265117724878207
>>END_MODULE
