SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913501_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 80631). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913501_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1267.838 s (17.100 µs/read; 3.51 M reads/minute). === Summary === Total reads processed: 74,143,100 Reads with adapters: 33,937,220 (45.8%) Reads written (passing filters): 74,143,100 (100.0%) Total basepairs processed: 11,121,465,000 bp Quality-trimmed: 11,151,258 bp (0.1%) Total written (filtered): 10,836,084,984 bp (97.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 33937220 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.2% C: 0.3% G: 16.2% T: 47.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 20976336 18535775.0 0 20976336 2 3310608 4633943.8 0 3310608 3 1406752 1158485.9 0 1406752 4 688349 289621.5 0 688349 5 184842 72405.4 0 184842 6 153867 18101.3 0 153867 7 159670 4525.3 0 159670 8 168630 1131.3 0 168630 9 168324 282.8 0 167515 809 10 168147 70.7 1 154706 13441 11 182120 17.7 1 160883 21237 12 175808 4.4 1 159229 16579 13 158783 1.1 1 129965 28818 14 169897 1.1 1 136600 33297 15 161260 1.1 1 132201 29059 16 167318 1.1 1 133842 33476 17 169003 1.1 1 135438 33565 18 160332 1.1 1 132354 27978 19 149414 1.1 1 122846 26568 20 154861 1.1 1 127117 27744 21 153028 1.1 1 125610 27418 22 147010 1.1 1 122117 24893 23 150099 1.1 1 124012 26087 24 151166 1.1 1 123659 27507 25 142499 1.1 1 118673 23826 26 141660 1.1 1 118431 23229 27 144197 1.1 1 120584 23613 28 133518 1.1 1 112530 20988 29 139535 1.1 1 115875 23660 30 134245 1.1 1 113579 20666 31 126737 1.1 1 106974 19763 32 125372 1.1 1 105973 19399 33 126427 1.1 1 104923 21504 34 127941 1.1 1 107754 20187 35 114899 1.1 1 97809 17090 36 113856 1.1 1 96393 17463 37 129180 1.1 1 110398 18782 38 99133 1.1 1 83842 15291 39 111424 1.1 1 93604 17820 40 110173 1.1 1 93459 16714 41 98659 1.1 1 84760 13899 42 100389 1.1 1 85204 15185 43 101899 1.1 1 86738 15161 44 112512 1.1 1 97471 15041 45 75828 1.1 1 63262 12566 46 87868 1.1 1 75394 12474 47 133795 1.1 1 116268 17527 48 41263 1.1 1 35200 6063 49 75977 1.1 1 66327 9650 50 81984 1.1 1 70844 11140 51 74112 1.1 1 64553 9559 52 74188 1.1 1 63978 10210 53 74219 1.1 1 63860 10359 54 68646 1.1 1 59875 8771 55 62070 1.1 1 54344 7726 56 62460 1.1 1 54298 8162 57 64902 1.1 1 56442 8460 58 55879 1.1 1 48501 7378 59 61040 1.1 1 53425 7615 60 57762 1.1 1 50517 7245 61 47481 1.1 1 41334 6147 62 49889 1.1 1 44325 5564 63 38653 1.1 1 33940 4713 64 46051 1.1 1 40593 5458 65 46062 1.1 1 40820 5242 66 37380 1.1 1 32956 4424 67 29465 1.1 1 25856 3609 68 36852 1.1 1 32674 4178 69 38211 1.1 1 33912 4299 70 41452 1.1 1 36822 4630 71 34834 1.1 1 30837 3997 72 61268 1.1 1 54480 6788 73 4810 1.1 1 4022 788 74 2960 1.1 1 2557 403 75 11291 1.1 1 10232 1059 76 16187 1.1 1 14468 1719 77 17102 1.1 1 15375 1727 78 16286 1.1 1 14617 1669 79 15242 1.1 1 13706 1536 80 14204 1.1 1 12775 1429 81 12809 1.1 1 11521 1288 82 11260 1.1 1 10172 1088 83 10251 1.1 1 9145 1106 84 9096 1.1 1 8156 940 85 8180 1.1 1 7324 856 86 7117 1.1 1 6385 732 87 6356 1.1 1 5712 644 88 5668 1.1 1 5092 576 89 4896 1.1 1 4406 490 90 4363 1.1 1 3925 438 91 3804 1.1 1 3384 420 92 3127 1.1 1 2823 304 93 2711 1.1 1 2415 296 94 2350 1.1 1 2105 245 95 2025 1.1 1 1816 209 96 1789 1.1 1 1586 203 97 1462 1.1 1 1297 165 98 1195 1.1 1 1052 143 99 1051 1.1 1 939 112 100 880 1.1 1 770 110 101 709 1.1 1 620 89 102 591 1.1 1 512 79 103 523 1.1 1 440 83 104 450 1.1 1 382 68 105 365 1.1 1 315 50 106 335 1.1 1 278 57 107 299 1.1 1 245 54 108 267 1.1 1 221 46 109 225 1.1 1 186 39 110 178 1.1 1 131 47 111 198 1.1 1 155 43 112 159 1.1 1 113 46 113 174 1.1 1 136 38 114 137 1.1 1 97 40 115 135 1.1 1 106 29 116 148 1.1 1 105 43 117 128 1.1 1 92 36 118 142 1.1 1 121 21 119 168 1.1 1 132 36 120 141 1.1 1 117 24 121 147 1.1 1 124 23 122 159 1.1 1 124 35 123 161 1.1 1 128 33 124 151 1.1 1 129 22 125 144 1.1 1 120 24 126 127 1.1 1 96 31 127 136 1.1 1 108 28 128 127 1.1 1 105 22 129 111 1.1 1 84 27 130 156 1.1 1 132 24 131 104 1.1 1 84 20 132 124 1.1 1 100 24 133 104 1.1 1 63 41 134 98 1.1 1 75 23 135 68 1.1 1 51 17 136 116 1.1 1 95 21 137 74 1.1 1 57 17 138 76 1.1 1 48 28 139 63 1.1 1 44 19 140 88 1.1 1 67 21 141 60 1.1 1 35 25 142 55 1.1 1 41 14 143 79 1.1 1 46 33 144 79 1.1 1 43 36 145 83 1.1 1 62 21 146 85 1.1 1 60 25 147 56 1.1 1 42 14 148 80 1.1 1 43 37 149 82 1.1 1 52 30 150 813 1.1 1 46 767 RUN STATISTICS FOR INPUT FILE: ERR13913501_1.fastq.gz ============================================= 74143100 sequences processed in total