SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913500_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 32013). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913500_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1515.229 s (17.290 µs/read; 3.47 M reads/minute). === Summary === Total reads processed: 87,636,960 Reads with adapters: 36,416,508 (41.6%) Reads written (passing filters): 87,636,960 (100.0%) Total basepairs processed: 13,145,544,000 bp Quality-trimmed: 13,757,534 bp (0.1%) Total written (filtered): 12,989,082,585 bp (98.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 36416508 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.8% C: 0.2% G: 14.1% T: 47.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 26876469 21909240.0 0 26876469 2 4038895 5477310.0 0 4038895 3 1582418 1369327.5 0 1582418 4 696031 342331.9 0 696031 5 87595 85583.0 0 87595 6 71591 21395.7 0 71591 7 72906 5348.9 0 72906 8 75930 1337.2 0 75930 9 76799 334.3 0 76025 774 10 76131 83.6 1 69771 6360 11 81137 20.9 1 72011 9126 12 77658 5.2 1 70460 7198 13 69516 1.3 1 62273 7243 14 74820 1.3 1 65266 9554 15 70751 1.3 1 62787 7964 16 73789 1.3 1 63802 9987 17 73672 1.3 1 63894 9778 18 68795 1.3 1 61503 7292 19 64427 1.3 1 57420 7007 20 65806 1.3 1 58626 7180 21 65564 1.3 1 58016 7548 22 62173 1.3 1 56096 6077 23 63315 1.3 1 56408 6907 24 63380 1.3 1 55797 7583 25 59605 1.3 1 53686 5919 26 58600 1.3 1 53025 5575 27 59702 1.3 1 53815 5887 28 55920 1.3 1 50949 4971 29 58061 1.3 1 51857 6204 30 55469 1.3 1 50619 4850 31 52081 1.3 1 47526 4555 32 51591 1.3 1 46986 4605 33 52024 1.3 1 46429 5595 34 51895 1.3 1 47077 4818 35 53204 1.3 1 49084 4120 36 41136 1.3 1 37148 3988 37 49568 1.3 1 44350 5218 38 45108 1.3 1 41387 3721 39 42528 1.3 1 39158 3370 40 42828 1.3 1 39128 3700 41 42852 1.3 1 39169 3683 42 39259 1.3 1 36197 3062 43 41345 1.3 1 37782 3563 44 38194 1.3 1 34861 3333 45 43213 1.3 1 37934 5279 46 37710 1.3 1 34143 3567 47 55431 1.3 1 51235 4196 48 20542 1.3 1 18587 1955 49 30728 1.3 1 28429 2299 50 56186 1.3 1 52130 4056 51 29728 1.3 1 27434 2294 52 37271 1.3 1 34235 3036 53 46942 1.3 1 43223 3719 54 22216 1.3 1 20604 1612 55 14334 1.3 1 13365 969 56 42514 1.3 1 39941 2573 57 33387 1.3 1 31473 1914 58 19817 1.3 1 18396 1421 59 26274 1.3 1 25009 1265 60 8114 1.3 1 7495 619 61 27425 1.3 1 25725 1700 62 10128 1.3 1 9435 693 63 5528 1.3 1 5038 490 64 21142 1.3 1 19989 1153 65 17395 1.3 1 16524 871 66 3998 1.3 1 3632 366 67 11305 1.3 1 10529 776 68 16295 1.3 1 15410 885 69 9167 1.3 1 8584 583 70 14747 1.3 1 13861 886 71 15036 1.3 1 14026 1010 72 27510 1.3 1 25970 1540 73 5356 1.3 1 4859 497 74 1299 1.3 1 1140 159 75 3130 1.3 1 2955 175 76 6179 1.3 1 5851 328 77 7198 1.3 1 6809 389 78 7265 1.3 1 6803 462 79 7055 1.3 1 6658 397 80 6498 1.3 1 6132 366 81 5997 1.3 1 5615 382 82 5321 1.3 1 5025 296 83 4607 1.3 1 4323 284 84 4218 1.3 1 3964 254 85 3721 1.3 1 3486 235 86 3206 1.3 1 2974 232 87 2813 1.3 1 2653 160 88 2390 1.3 1 2225 165 89 2162 1.3 1 2011 151 90 1844 1.3 1 1707 137 91 1567 1.3 1 1448 119 92 1411 1.3 1 1309 102 93 1023 1.3 1 952 71 94 871 1.3 1 791 80 95 819 1.3 1 744 75 96 612 1.3 1 548 64 97 546 1.3 1 490 56 98 432 1.3 1 375 57 99 376 1.3 1 330 46 100 334 1.3 1 289 45 101 263 1.3 1 209 54 102 218 1.3 1 188 30 103 180 1.3 1 152 28 104 184 1.3 1 150 34 105 128 1.3 1 102 26 106 147 1.3 1 112 35 107 129 1.3 1 96 33 108 96 1.3 1 80 16 109 105 1.3 1 87 18 110 83 1.3 1 56 27 111 84 1.3 1 60 24 112 90 1.3 1 67 23 113 78 1.3 1 58 20 114 96 1.3 1 70 26 115 70 1.3 1 42 28 116 71 1.3 1 53 18 117 66 1.3 1 46 20 118 78 1.3 1 60 18 119 66 1.3 1 48 18 120 48 1.3 1 33 15 121 68 1.3 1 45 23 122 81 1.3 1 61 20 123 55 1.3 1 45 10 124 77 1.3 1 57 20 125 55 1.3 1 46 9 126 56 1.3 1 47 9 127 50 1.3 1 44 6 128 57 1.3 1 49 8 129 51 1.3 1 41 10 130 46 1.3 1 39 7 131 52 1.3 1 43 9 132 33 1.3 1 27 6 133 33 1.3 1 23 10 134 32 1.3 1 28 4 135 23 1.3 1 19 4 136 26 1.3 1 21 5 137 21 1.3 1 17 4 138 20 1.3 1 15 5 139 19 1.3 1 13 6 140 18 1.3 1 15 3 141 14 1.3 1 9 5 142 10 1.3 1 5 5 143 12 1.3 1 10 2 144 12 1.3 1 8 4 145 18 1.3 1 13 5 146 13 1.3 1 7 6 147 12 1.3 1 8 4 148 11 1.3 1 6 5 149 23 1.3 1 4 19 150 359 1.3 1 8 351 RUN STATISTICS FOR INPUT FILE: ERR13913500_1.fastq.gz ============================================= 87636960 sequences processed in total