Bismark report for: ERR13913499_val_1.fq.gz and ERR13913499_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 104928714 Number of paired-end alignments with a unique best hit: 50256230 Mapping efficiency: 47.9% Sequence pairs with no alignments under any condition: 46427415 Sequence pairs did not map uniquely: 8245069 Sequence pairs which were discarded because genomic sequence could not be extracted: 17 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25123512 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 25132701 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2464239968 Total methylated C's in CpG context: 33489446 Total methylated C's in CHG context: 1459845 Total methylated C's in CHH context: 5386430 Total methylated C's in Unknown context: 82545 Total unmethylated C's in CpG context: 259995863 Total unmethylated C's in CHG context: 406707985 Total unmethylated C's in CHH context: 1757200399 Total unmethylated C's in Unknown context: 7179778 C methylated in CpG context: 11.4% C methylated in CHG context: 0.4% C methylated in CHH context: 0.3% C methylated in Unknown context (CN or CHN): 1.1% Bismark completed in 0d 4h 57m 54s