SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913499_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 27813). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913499_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2225.990 s (21.210 µs/read; 2.83 M reads/minute). === Summary === Total reads processed: 104,947,850 Reads with adapters: 43,103,575 (41.1%) Reads written (passing filters): 104,947,850 (100.0%) Total basepairs processed: 15,742,177,500 bp Quality-trimmed: 15,090,307 bp (0.1%) Total written (filtered): 15,572,339,014 bp (98.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 43103575 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.2% G: 14.2% T: 48.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 32081713 26236962.5 0 32081713 2 4869639 6559240.6 0 4869639 3 1920931 1639810.2 0 1920931 4 841193 409952.5 0 841193 5 95403 102488.1 0 95403 6 79388 25622.0 0 79388 7 80855 6405.5 0 80855 8 85111 1601.4 0 85111 9 83204 400.3 0 82203 1001 10 82872 100.1 1 75737 7135 11 89350 25.0 1 78857 10493 12 84379 6.3 1 76532 7847 13 76089 1.6 1 68001 8088 14 80483 1.6 1 70287 10196 15 76414 1.6 1 67772 8642 16 77596 1.6 1 67184 10412 17 79949 1.6 1 69532 10417 18 75003 1.6 1 66899 8104 19 68713 1.6 1 61398 7315 20 70930 1.6 1 63218 7712 21 69605 1.6 1 61774 7831 22 66299 1.6 1 59809 6490 23 67009 1.6 1 59835 7174 24 67929 1.6 1 59866 8063 25 63344 1.6 1 57136 6208 26 62441 1.6 1 56460 5981 27 64218 1.6 1 57964 6254 28 58913 1.6 1 53605 5308 29 61948 1.6 1 55508 6440 30 59780 1.6 1 54655 5125 31 56033 1.6 1 51168 4865 32 54501 1.6 1 49807 4694 33 54482 1.6 1 48693 5789 34 50901 1.6 1 46625 4276 35 52166 1.6 1 46926 5240 36 52227 1.6 1 47079 5148 37 46117 1.6 1 42370 3747 38 45232 1.6 1 41584 3648 39 46622 1.6 1 42267 4355 40 47619 1.6 1 43610 4009 41 41549 1.6 1 37700 3849 42 42962 1.6 1 38962 4000 43 43057 1.6 1 39211 3846 44 44559 1.6 1 41470 3089 45 33639 1.6 1 29781 3858 46 36029 1.6 1 32996 3033 47 55884 1.6 1 51932 3952 48 16683 1.6 1 15132 1551 49 31772 1.6 1 29682 2090 50 39189 1.6 1 36179 3010 51 28791 1.6 1 26721 2070 52 33088 1.6 1 30270 2818 53 34444 1.6 1 31528 2916 54 26119 1.6 1 24362 1757 55 22169 1.6 1 20704 1465 56 28414 1.6 1 26304 2110 57 30187 1.6 1 28187 2000 58 21983 1.6 1 20420 1563 59 30299 1.6 1 28535 1764 60 18463 1.6 1 17199 1264 61 23118 1.6 1 21539 1579 62 18254 1.6 1 17257 997 63 11419 1.6 1 10599 820 64 20934 1.6 1 19697 1237 65 21363 1.6 1 20257 1106 66 10626 1.6 1 9933 693 67 11614 1.6 1 10856 758 68 16299 1.6 1 15371 928 69 13568 1.6 1 12775 793 70 16217 1.6 1 15218 999 71 15368 1.6 1 14379 989 72 27591 1.6 1 25960 1631 73 2796 1.6 1 2450 346 74 1125 1.6 1 978 147 75 3986 1.6 1 3773 213 76 6651 1.6 1 6309 342 77 7299 1.6 1 6891 408 78 7120 1.6 1 6706 414 79 6754 1.6 1 6377 377 80 6136 1.6 1 5779 357 81 5711 1.6 1 5345 366 82 5358 1.6 1 5023 335 83 4655 1.6 1 4337 318 84 4077 1.6 1 3825 252 85 3609 1.6 1 3372 237 86 3144 1.6 1 2939 205 87 2818 1.6 1 2630 188 88 2420 1.6 1 2269 151 89 2268 1.6 1 2105 163 90 1856 1.6 1 1730 126 91 1679 1.6 1 1552 127 92 1380 1.6 1 1269 111 93 1122 1.6 1 1021 101 94 1061 1.6 1 986 75 95 848 1.6 1 772 76 96 687 1.6 1 626 61 97 623 1.6 1 552 71 98 501 1.6 1 438 63 99 442 1.6 1 390 52 100 370 1.6 1 324 46 101 314 1.6 1 254 60 102 287 1.6 1 240 47 103 241 1.6 1 200 41 104 204 1.6 1 153 51 105 190 1.6 1 146 44 106 176 1.6 1 143 33 107 160 1.6 1 123 37 108 145 1.6 1 107 38 109 139 1.6 1 98 41 110 130 1.6 1 92 38 111 133 1.6 1 92 41 112 114 1.6 1 86 28 113 97 1.6 1 72 25 114 105 1.6 1 76 29 115 82 1.6 1 56 26 116 86 1.6 1 61 25 117 80 1.6 1 61 19 118 109 1.6 1 78 31 119 107 1.6 1 74 33 120 66 1.6 1 51 15 121 86 1.6 1 67 19 122 84 1.6 1 63 21 123 71 1.6 1 49 22 124 69 1.6 1 54 15 125 70 1.6 1 51 19 126 54 1.6 1 38 16 127 58 1.6 1 38 20 128 52 1.6 1 38 14 129 66 1.6 1 48 18 130 53 1.6 1 31 22 131 50 1.6 1 39 11 132 33 1.6 1 25 8 133 65 1.6 1 32 33 134 29 1.6 1 17 12 135 30 1.6 1 22 8 136 31 1.6 1 20 11 137 35 1.6 1 22 13 138 40 1.6 1 18 22 139 29 1.6 1 13 16 140 24 1.6 1 11 13 141 19 1.6 1 6 13 142 17 1.6 1 7 10 143 43 1.6 1 10 33 144 31 1.6 1 10 21 145 32 1.6 1 18 14 146 26 1.6 1 15 11 147 23 1.6 1 5 18 148 32 1.6 1 12 20 149 35 1.6 1 8 27 150 575 1.6 1 10 565 RUN STATISTICS FOR INPUT FILE: ERR13913499_1.fastq.gz ============================================= 104947850 sequences processed in total