SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913498_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 16843). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913498_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 2501.433 s (22.247 µs/read; 2.70 M reads/minute). === Summary === Total reads processed: 112,438,079 Reads with adapters: 57,695,167 (51.3%) Reads written (passing filters): 112,438,079 (100.0%) Total basepairs processed: 16,865,711,850 bp Quality-trimmed: 70,006,840 bp (0.4%) Total written (filtered): 16,669,306,361 bp (98.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 57695167 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 52.5% C: 17.3% G: 0.4% T: 29.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 54970040 28109519.8 0 54970040 2 137250 7027379.9 0 137250 3 121953 1756845.0 0 121953 4 87926 439211.2 0 87926 5 79326 109802.8 0 79326 6 61487 27450.7 0 61487 7 66251 6862.7 0 66251 8 71286 1715.7 0 71286 9 53574 428.9 0 53543 31 10 65677 107.2 1 60953 4724 11 63314 26.8 1 57924 5390 12 61344 6.7 1 43681 17663 13 57618 1.7 1 40689 16929 14 62937 1.7 1 44809 18128 15 52223 1.7 1 37293 14930 16 55121 1.7 1 39172 15949 17 67539 1.7 1 48318 19221 18 39677 1.7 1 28341 11336 19 68318 1.7 1 49721 18597 20 45500 1.7 1 32220 13280 21 40896 1.7 1 29363 11533 22 47934 1.7 1 34739 13195 23 46456 1.7 1 33771 12685 24 54459 1.7 1 39602 14857 25 50177 1.7 1 36346 13831 26 36521 1.7 1 26633 9888 27 41291 1.7 1 30019 11272 28 42800 1.7 1 31825 10975 29 40606 1.7 1 29695 10911 30 41270 1.7 1 30508 10762 31 40112 1.7 1 29663 10449 32 37310 1.7 1 27840 9470 33 37987 1.7 1 28153 9834 34 35727 1.7 1 26566 9161 35 37553 1.7 1 28224 9329 36 34354 1.7 1 25603 8751 37 29930 1.7 1 22389 7541 38 30160 1.7 1 22788 7372 39 32860 1.7 1 24868 7992 40 27776 1.7 1 20918 6858 41 28324 1.7 1 21402 6922 42 32549 1.7 1 24862 7687 43 21775 1.7 1 16448 5327 44 26393 1.7 1 20239 6154 45 23247 1.7 1 17809 5438 46 23873 1.7 1 18317 5556 47 23782 1.7 1 18168 5614 48 23409 1.7 1 17875 5534 49 20809 1.7 1 16123 4686 50 20983 1.7 1 16030 4953 51 20603 1.7 1 15936 4667 52 19009 1.7 1 14755 4254 53 18471 1.7 1 14308 4163 54 18198 1.7 1 14040 4158 55 16715 1.7 1 13103 3612 56 17627 1.7 1 13954 3673 57 14115 1.7 1 10924 3191 58 15086 1.7 1 11867 3219 59 17947 1.7 1 14223 3724 60 11317 1.7 1 8820 2497 61 13160 1.7 1 10438 2722 62 13516 1.7 1 10779 2737 63 11948 1.7 1 9488 2460 64 10857 1.7 1 8574 2283 65 19317 1.7 1 15566 3751 66 7966 1.7 1 6241 1725 67 7170 1.7 1 5701 1469 68 7114 1.7 1 5751 1363 69 9957 1.7 1 8055 1902 70 9610 1.7 1 7549 2061 71 7496 1.7 1 6047 1449 72 7982 1.7 1 6434 1548 73 7593 1.7 1 6129 1464 74 7130 1.7 1 5697 1433 75 9864 1.7 1 7886 1978 76 5816 1.7 1 4582 1234 77 4203 1.7 1 3314 889 78 1873 1.7 1 1511 362 79 2896 1.7 1 2397 499 80 3414 1.7 1 2776 638 81 3300 1.7 1 2686 614 82 3139 1.7 1 2559 580 83 2963 1.7 1 2401 562 84 2590 1.7 1 2081 509 85 2401 1.7 1 1909 492 86 2172 1.7 1 1732 440 87 2053 1.7 1 1630 423 88 1783 1.7 1 1373 410 89 1577 1.7 1 1223 354 90 1392 1.7 1 1074 318 91 1224 1.7 1 980 244 92 1164 1.7 1 885 279 93 1018 1.7 1 746 272 94 916 1.7 1 646 270 95 779 1.7 1 559 220 96 658 1.7 1 453 205 97 625 1.7 1 419 206 98 564 1.7 1 387 177 99 483 1.7 1 325 158 100 507 1.7 1 334 173 101 428 1.7 1 264 164 102 412 1.7 1 248 164 103 348 1.7 1 209 139 104 284 1.7 1 175 109 105 285 1.7 1 175 110 106 270 1.7 1 147 123 107 259 1.7 1 141 118 108 228 1.7 1 126 102 109 248 1.7 1 141 107 110 209 1.7 1 106 103 111 218 1.7 1 104 114 112 185 1.7 1 101 84 113 191 1.7 1 98 93 114 180 1.7 1 102 78 115 168 1.7 1 101 67 116 208 1.7 1 127 81 117 155 1.7 1 88 67 118 168 1.7 1 90 78 119 142 1.7 1 76 66 120 162 1.7 1 91 71 121 147 1.7 1 79 68 122 124 1.7 1 72 52 123 99 1.7 1 56 43 124 134 1.7 1 74 60 125 110 1.7 1 55 55 126 119 1.7 1 58 61 127 90 1.7 1 49 41 128 95 1.7 1 52 43 129 68 1.7 1 35 33 130 93 1.7 1 62 31 131 61 1.7 1 40 21 132 52 1.7 1 29 23 133 29 1.7 1 20 9 134 30 1.7 1 18 12 135 36 1.7 1 22 14 136 28 1.7 1 18 10 137 14 1.7 1 13 1 138 17 1.7 1 11 6 139 7 1.7 1 6 1 140 11 1.7 1 6 5 141 5 1.7 1 3 2 142 12 1.7 1 4 8 143 2 1.7 1 2 144 9 1.7 1 5 4 145 13 1.7 1 5 8 146 10 1.7 1 5 5 147 18 1.7 1 7 11 148 6 1.7 1 5 1 149 7 1.7 1 0 7 150 11 1.7 1 3 8 RUN STATISTICS FOR INPUT FILE: ERR13913498_2.fastq.gz ============================================= 112438079 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 112438079 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 25743 (0.02%)