SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913498_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 16843). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913498_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2483.171 s (22.085 µs/read; 2.72 M reads/minute). === Summary === Total reads processed: 112,438,079 Reads with adapters: 45,351,000 (40.3%) Reads written (passing filters): 112,438,079 (100.0%) Total basepairs processed: 16,865,711,850 bp Quality-trimmed: 23,349,529 bp (0.1%) Total written (filtered): 16,719,751,691 bp (99.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 45351000 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.5% C: 0.2% G: 13.6% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 34975429 28109519.8 0 34975429 2 5203633 7027379.9 0 5203633 3 2021131 1756845.0 0 2021131 4 842738 439211.2 0 842738 5 74718 109802.8 0 74718 6 54059 27450.7 0 54059 7 56709 6862.7 0 56709 8 61435 1715.7 0 61435 9 59167 428.9 0 58212 955 10 60207 107.2 1 53580 6627 11 66188 26.8 1 56345 9843 12 61235 6.7 1 54075 7160 13 54527 1.7 1 48000 6527 14 57265 1.7 1 49387 7878 15 54848 1.7 1 47530 7318 16 56413 1.7 1 47683 8730 17 59044 1.7 1 50474 8570 18 53908 1.7 1 48017 5891 19 45426 1.7 1 39819 5607 20 50844 1.7 1 44251 6593 21 48402 1.7 1 42410 5992 22 46475 1.7 1 41225 5250 23 47827 1.7 1 41771 6056 24 46900 1.7 1 40187 6713 25 44535 1.7 1 39408 5127 26 42700 1.7 1 37933 4767 27 43744 1.7 1 38606 5138 28 40212 1.7 1 36064 4148 29 43071 1.7 1 37419 5652 30 42511 1.7 1 38174 4337 31 35147 1.7 1 31604 3543 32 36968 1.7 1 33233 3735 33 36575 1.7 1 32484 4091 34 34448 1.7 1 31020 3428 35 36514 1.7 1 32070 4444 36 35771 1.7 1 32718 3053 37 27690 1.7 1 25246 2444 38 30222 1.7 1 27104 3118 39 31658 1.7 1 27845 3813 40 28145 1.7 1 25715 2430 41 30155 1.7 1 26363 3792 42 31395 1.7 1 27891 3504 43 23546 1.7 1 21639 1907 44 24310 1.7 1 22328 1982 45 29089 1.7 1 25520 3569 46 24689 1.7 1 21649 3040 47 42847 1.7 1 39061 3786 48 15479 1.7 1 13380 2099 49 17759 1.7 1 16275 1484 50 37316 1.7 1 34767 2549 51 16317 1.7 1 14844 1473 52 22355 1.7 1 20329 2026 53 28236 1.7 1 25624 2612 54 15541 1.7 1 14361 1180 55 8319 1.7 1 7763 556 56 21350 1.7 1 20071 1279 57 21760 1.7 1 20552 1208 58 10761 1.7 1 9864 897 59 16481 1.7 1 15681 800 60 5733 1.7 1 5250 483 61 13630 1.7 1 12668 962 62 6197 1.7 1 5838 359 63 3463 1.7 1 3105 358 64 12138 1.7 1 11491 647 65 9948 1.7 1 9506 442 66 3050 1.7 1 2761 289 67 5574 1.7 1 5104 470 68 10438 1.7 1 9882 556 69 5141 1.7 1 4779 362 70 8629 1.7 1 8042 587 71 8483 1.7 1 7859 624 72 13169 1.7 1 12298 871 73 1214 1.7 1 968 246 74 774 1.7 1 659 115 75 2290 1.7 1 2138 152 76 3626 1.7 1 3402 224 77 4317 1.7 1 4052 265 78 4126 1.7 1 3867 259 79 4087 1.7 1 3817 270 80 3845 1.7 1 3569 276 81 3547 1.7 1 3281 266 82 3280 1.7 1 3038 242 83 3011 1.7 1 2767 244 84 2559 1.7 1 2344 215 85 2303 1.7 1 2108 195 86 2113 1.7 1 1923 190 87 1905 1.7 1 1731 174 88 1709 1.7 1 1554 155 89 1475 1.7 1 1305 170 90 1315 1.7 1 1168 147 91 1146 1.7 1 1020 126 92 1062 1.7 1 951 111 93 893 1.7 1 796 97 94 794 1.7 1 680 114 95 675 1.7 1 567 108 96 586 1.7 1 486 100 97 528 1.7 1 432 96 98 479 1.7 1 379 100 99 401 1.7 1 335 66 100 361 1.7 1 287 74 101 332 1.7 1 241 91 102 303 1.7 1 233 70 103 262 1.7 1 196 66 104 207 1.7 1 151 56 105 202 1.7 1 146 56 106 183 1.7 1 122 61 107 183 1.7 1 136 47 108 165 1.7 1 109 56 109 166 1.7 1 111 55 110 159 1.7 1 105 54 111 133 1.7 1 88 45 112 130 1.7 1 87 43 113 119 1.7 1 80 39 114 130 1.7 1 84 46 115 99 1.7 1 61 38 116 147 1.7 1 99 48 117 108 1.7 1 69 39 118 103 1.7 1 61 42 119 98 1.7 1 65 33 120 118 1.7 1 86 32 121 109 1.7 1 75 34 122 77 1.7 1 58 19 123 83 1.7 1 51 32 124 77 1.7 1 42 35 125 63 1.7 1 41 22 126 83 1.7 1 57 26 127 58 1.7 1 34 24 128 64 1.7 1 43 21 129 56 1.7 1 38 18 130 74 1.7 1 58 16 131 48 1.7 1 34 14 132 44 1.7 1 36 8 133 26 1.7 1 23 3 134 37 1.7 1 20 17 135 31 1.7 1 22 9 136 22 1.7 1 17 5 137 19 1.7 1 10 9 138 21 1.7 1 11 10 139 13 1.7 1 4 9 140 12 1.7 1 8 4 141 4 1.7 1 3 1 142 11 1.7 1 7 4 143 8 1.7 1 2 6 144 11 1.7 1 5 6 145 14 1.7 1 9 5 146 8 1.7 1 4 4 147 25 1.7 1 11 14 148 10 1.7 1 6 4 149 21 1.7 1 4 17 150 366 1.7 1 3 363 RUN STATISTICS FOR INPUT FILE: ERR13913498_1.fastq.gz ============================================= 112438079 sequences processed in total