SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913497_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 17551). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913497_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 2740.823 s (19.412 µs/read; 3.09 M reads/minute). === Summary === Total reads processed: 141,189,139 Reads with adapters: 72,417,934 (51.3%) Reads written (passing filters): 141,189,139 (100.0%) Total basepairs processed: 21,178,370,850 bp Quality-trimmed: 85,343,014 bp (0.4%) Total written (filtered): 20,934,487,653 bp (98.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 72417934 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 52.6% C: 17.1% G: 0.4% T: 30.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 69012910 35297284.8 0 69012910 2 161288 8824321.2 0 161288 3 147817 2206080.3 0 147817 4 108228 551520.1 0 108228 5 96775 137880.0 0 96775 6 76051 34470.0 0 76051 7 81565 8617.5 0 81565 8 86024 2154.4 0 86024 9 67983 538.6 0 67948 35 10 80865 134.6 1 75474 5391 11 78451 33.7 1 71717 6734 12 76358 8.4 1 54919 21439 13 72713 2.1 1 52063 20650 14 77816 2.1 1 56443 21373 15 66408 2.1 1 47793 18615 16 69899 2.1 1 50270 19629 17 83529 2.1 1 60689 22840 18 52026 2.1 1 37771 14255 19 83505 2.1 1 61669 21836 20 59693 2.1 1 43020 16673 21 53624 2.1 1 38895 14729 22 61024 2.1 1 44895 16129 23 59340 2.1 1 43785 15555 24 76499 2.1 1 57063 19436 25 66276 2.1 1 48276 18000 26 43679 2.1 1 32387 11292 27 46481 2.1 1 34075 12406 28 57231 2.1 1 43393 13838 29 48160 2.1 1 35564 12596 30 56484 2.1 1 42667 13817 31 44125 2.1 1 32910 11215 32 58006 2.1 1 44392 13614 33 45105 2.1 1 33803 11302 34 43861 2.1 1 33217 10644 35 43389 2.1 1 32821 10568 36 40590 2.1 1 30701 9889 37 40181 2.1 1 30528 9653 38 40016 2.1 1 30905 9111 39 41460 2.1 1 31729 9731 40 37536 2.1 1 28737 8799 41 38836 2.1 1 30116 8720 42 33606 2.1 1 25668 7938 43 33074 2.1 1 25179 7895 44 32582 2.1 1 25301 7281 45 30815 2.1 1 23823 6992 46 30445 2.1 1 23690 6755 47 30460 2.1 1 23561 6899 48 30932 2.1 1 24180 6752 49 26050 2.1 1 20285 5765 50 26271 2.1 1 20313 5958 51 26543 2.1 1 20676 5867 52 23450 2.1 1 18420 5030 53 23429 2.1 1 18482 4947 54 23030 2.1 1 17974 5056 55 20852 2.1 1 16517 4335 56 22643 2.1 1 18193 4450 57 17813 2.1 1 13984 3829 58 18957 2.1 1 15104 3853 59 22093 2.1 1 17627 4466 60 15095 2.1 1 11901 3194 61 16127 2.1 1 12823 3304 62 16476 2.1 1 13148 3328 63 14669 2.1 1 11713 2956 64 13834 2.1 1 11046 2788 65 24159 2.1 1 19743 4416 66 9381 2.1 1 7325 2056 67 8719 2.1 1 6891 1828 68 9045 2.1 1 7338 1707 69 12166 2.1 1 9837 2329 70 11790 2.1 1 9279 2511 71 8717 2.1 1 7092 1625 72 9452 2.1 1 7676 1776 73 8864 2.1 1 7146 1718 74 8315 2.1 1 6634 1681 75 11917 2.1 1 9565 2352 76 6661 2.1 1 5326 1335 77 4592 2.1 1 3595 997 78 2102 2.1 1 1661 441 79 3324 2.1 1 2729 595 80 3872 2.1 1 3146 726 81 3679 2.1 1 2997 682 82 3570 2.1 1 2875 695 83 3340 2.1 1 2671 669 84 2898 2.1 1 2282 616 85 2771 2.1 1 2175 596 86 2314 2.1 1 1809 505 87 2289 2.1 1 1805 484 88 1922 2.1 1 1480 442 89 1803 2.1 1 1361 442 90 1544 2.1 1 1166 378 91 1409 2.1 1 1041 368 92 1241 2.1 1 926 315 93 1062 2.1 1 759 303 94 1013 2.1 1 700 313 95 930 2.1 1 636 294 96 759 2.1 1 475 284 97 707 2.1 1 458 249 98 666 2.1 1 412 254 99 585 2.1 1 379 206 100 592 2.1 1 361 231 101 518 2.1 1 309 209 102 465 2.1 1 267 198 103 450 2.1 1 269 181 104 442 2.1 1 243 199 105 412 2.1 1 228 184 106 382 2.1 1 223 159 107 336 2.1 1 191 145 108 348 2.1 1 200 148 109 320 2.1 1 175 145 110 295 2.1 1 160 135 111 290 2.1 1 152 138 112 304 2.1 1 171 133 113 258 2.1 1 126 132 114 287 2.1 1 170 117 115 267 2.1 1 149 118 116 250 2.1 1 141 109 117 227 2.1 1 137 90 118 219 2.1 1 123 96 119 232 2.1 1 135 97 120 237 2.1 1 132 105 121 217 2.1 1 116 101 122 192 2.1 1 103 89 123 174 2.1 1 85 89 124 208 2.1 1 125 83 125 163 2.1 1 105 58 126 173 2.1 1 99 74 127 158 2.1 1 95 63 128 121 2.1 1 80 41 129 103 2.1 1 64 39 130 127 2.1 1 75 52 131 72 2.1 1 52 20 132 69 2.1 1 52 17 133 52 2.1 1 41 11 134 53 2.1 1 37 16 135 46 2.1 1 34 12 136 30 2.1 1 20 10 137 28 2.1 1 19 9 138 33 2.1 1 19 14 139 16 2.1 1 11 5 140 12 2.1 1 10 2 141 20 2.1 1 10 10 142 10 2.1 1 5 5 143 12 2.1 1 8 4 144 17 2.1 1 12 5 145 6 2.1 1 2 4 146 10 2.1 1 2 8 147 13 2.1 1 5 8 148 14 2.1 1 10 4 149 12 2.1 1 8 4 150 11 2.1 1 0 11 RUN STATISTICS FOR INPUT FILE: ERR13913497_2.fastq.gz ============================================= 141189139 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 141189139 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 27615 (0.02%)