SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913497_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 17551). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913497_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2631.332 s (18.637 µs/read; 3.22 M reads/minute). === Summary === Total reads processed: 141,189,139 Reads with adapters: 57,037,100 (40.4%) Reads written (passing filters): 141,189,139 (100.0%) Total basepairs processed: 21,178,370,850 bp Quality-trimmed: 28,658,320 bp (0.1%) Total written (filtered): 20,994,904,573 bp (99.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 57037100 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.1% G: 13.7% T: 48.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 43907054 35297284.8 0 43907054 2 6604049 8824321.2 0 6604049 3 2565182 2206080.3 0 2565182 4 1062517 551520.1 0 1062517 5 92388 137880.0 0 92388 6 66746 34470.0 0 66746 7 70070 8617.5 0 70070 8 74015 2154.4 0 74015 9 73766 538.6 0 72537 1229 10 75675 134.6 1 67218 8457 11 81458 33.7 1 69438 12020 12 75337 8.4 1 66835 8502 13 68902 2.1 1 60485 8417 14 71346 2.1 1 61072 10274 15 68292 2.1 1 59360 8932 16 71138 2.1 1 59876 11262 17 74156 2.1 1 63670 10486 18 67161 2.1 1 59873 7288 19 58086 2.1 1 50908 7178 20 64179 2.1 1 56037 8142 21 61870 2.1 1 54150 7720 22 58899 2.1 1 52273 6626 23 60121 2.1 1 52629 7492 24 60836 2.1 1 51972 8864 25 56369 2.1 1 49767 6602 26 54843 2.1 1 48468 6375 27 56864 2.1 1 50134 6730 28 50266 2.1 1 45180 5086 29 54596 2.1 1 47394 7202 30 51558 2.1 1 46218 5340 31 44985 2.1 1 40338 4647 32 46887 2.1 1 42015 4872 33 47135 2.1 1 41460 5675 34 48978 2.1 1 43411 5567 35 39347 2.1 1 35761 3586 36 41465 2.1 1 37344 4121 37 44832 2.1 1 40307 4525 38 40866 2.1 1 36641 4225 39 37528 2.1 1 32791 4737 40 36687 2.1 1 33266 3421 41 34306 2.1 1 31407 2899 42 34963 2.1 1 31224 3739 43 35823 2.1 1 32348 3475 44 34841 2.1 1 31891 2950 45 28953 2.1 1 25538 3415 46 31460 2.1 1 28002 3458 47 44620 2.1 1 40962 3658 48 14913 2.1 1 13099 1814 49 23978 2.1 1 22175 1803 50 28911 2.1 1 26474 2437 51 23298 2.1 1 21417 1881 52 24659 2.1 1 22178 2481 53 26956 2.1 1 24020 2936 54 21999 2.1 1 20162 1837 55 17986 2.1 1 16719 1267 56 20466 2.1 1 18810 1656 57 22777 2.1 1 20974 1803 58 16998 2.1 1 15431 1567 59 21934 2.1 1 20399 1535 60 17288 2.1 1 15999 1289 61 15424 2.1 1 13962 1462 62 14009 2.1 1 13265 744 63 9864 2.1 1 9019 845 64 15777 2.1 1 14672 1105 65 15410 2.1 1 14597 813 66 9689 2.1 1 8942 747 67 8329 2.1 1 7635 694 68 13200 2.1 1 12330 870 69 11301 2.1 1 10534 767 70 14235 2.1 1 13232 1003 71 10763 2.1 1 9898 865 72 15211 2.1 1 14027 1184 73 1181 2.1 1 947 234 74 1238 2.1 1 1085 153 75 3473 2.1 1 3255 218 76 4829 2.1 1 4514 315 77 5023 2.1 1 4678 345 78 4886 2.1 1 4521 365 79 4680 2.1 1 4312 368 80 4437 2.1 1 4095 342 81 4015 2.1 1 3734 281 82 3626 2.1 1 3325 301 83 3405 2.1 1 3112 293 84 2885 2.1 1 2630 255 85 2617 2.1 1 2341 276 86 2239 2.1 1 2030 209 87 2115 2.1 1 1917 198 88 1793 2.1 1 1576 217 89 1649 2.1 1 1452 197 90 1440 2.1 1 1244 196 91 1311 2.1 1 1124 187 92 1053 2.1 1 898 155 93 930 2.1 1 789 141 94 899 2.1 1 753 146 95 735 2.1 1 605 130 96 664 2.1 1 523 141 97 560 2.1 1 438 122 98 515 2.1 1 406 109 99 440 2.1 1 339 101 100 467 2.1 1 354 113 101 364 2.1 1 266 98 102 361 2.1 1 244 117 103 331 2.1 1 226 105 104 331 2.1 1 241 90 105 296 2.1 1 196 100 106 259 2.1 1 181 78 107 236 2.1 1 153 83 108 221 2.1 1 157 64 109 219 2.1 1 145 74 110 188 2.1 1 127 61 111 187 2.1 1 119 68 112 227 2.1 1 153 74 113 160 2.1 1 107 53 114 183 2.1 1 114 69 115 185 2.1 1 107 78 116 177 2.1 1 120 57 117 136 2.1 1 88 48 118 176 2.1 1 122 54 119 155 2.1 1 105 50 120 149 2.1 1 86 63 121 160 2.1 1 102 58 122 127 2.1 1 95 32 123 116 2.1 1 74 42 124 141 2.1 1 92 49 125 127 2.1 1 84 43 126 140 2.1 1 85 55 127 122 2.1 1 80 42 128 98 2.1 1 65 33 129 76 2.1 1 52 24 130 94 2.1 1 62 32 131 64 2.1 1 48 16 132 62 2.1 1 50 12 133 50 2.1 1 33 17 134 63 2.1 1 45 18 135 43 2.1 1 32 11 136 38 2.1 1 26 12 137 34 2.1 1 23 11 138 33 2.1 1 18 15 139 16 2.1 1 11 5 140 21 2.1 1 11 10 141 23 2.1 1 12 11 142 17 2.1 1 6 11 143 24 2.1 1 10 14 144 23 2.1 1 12 11 145 13 2.1 1 5 8 146 15 2.1 1 6 9 147 16 2.1 1 7 9 148 38 2.1 1 10 28 149 53 2.1 1 10 43 150 817 2.1 1 1 816 RUN STATISTICS FOR INPUT FILE: ERR13913497_1.fastq.gz ============================================= 141189139 sequences processed in total