SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913496_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 23102). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913496_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 3699.123 s (21.988 µs/read; 2.73 M reads/minute). === Summary === Total reads processed: 168,235,405 Reads with adapters: 68,552,047 (40.7%) Reads written (passing filters): 168,235,405 (100.0%) Total basepairs processed: 25,235,310,750 bp Quality-trimmed: 35,161,692 bp (0.1%) Total written (filtered): 24,981,440,624 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 68552047 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.2% G: 13.8% T: 48.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 52113285 42058851.2 0 52113285 2 7741711 10514712.8 0 7741711 3 3025864 2628678.2 0 3025864 4 1273656 657169.6 0 1273656 5 127736 164292.4 0 127736 6 92288 41073.1 0 92288 7 98644 10268.3 0 98644 8 104086 2567.1 0 104086 9 103854 641.8 0 102311 1543 10 104441 160.4 1 93383 11058 11 115502 40.1 1 98465 17037 12 107197 10.0 1 95147 12050 13 97474 2.5 1 84704 12770 14 102793 2.5 1 87441 15352 15 98989 2.5 1 85045 13944 16 101385 2.5 1 85013 16372 17 106136 2.5 1 90156 15980 18 97137 2.5 1 85752 11385 19 83042 2.5 1 72626 10416 20 92754 2.5 1 80384 12370 21 89368 2.5 1 77863 11505 22 86013 2.5 1 75813 10200 23 87852 2.5 1 76468 11384 24 88066 2.5 1 74714 13352 25 83290 2.5 1 72770 10520 26 79896 2.5 1 70429 9467 27 83309 2.5 1 72764 10545 28 76126 2.5 1 67366 8760 29 79928 2.5 1 68734 11194 30 77959 2.5 1 69422 8537 31 67399 2.5 1 60043 7356 32 71875 2.5 1 63986 7889 33 71457 2.5 1 62688 8769 34 75097 2.5 1 65755 9342 35 64947 2.5 1 57922 7025 36 62797 2.5 1 56781 6016 37 61638 2.5 1 54997 6641 38 66592 2.5 1 58880 7712 39 56884 2.5 1 51215 5669 40 57455 2.5 1 51706 5749 41 55114 2.5 1 49747 5367 42 56049 2.5 1 50725 5324 43 56907 2.5 1 50942 5965 44 52848 2.5 1 48006 4842 45 49515 2.5 1 43504 6011 46 51047 2.5 1 45169 5878 47 70590 2.5 1 64929 5661 48 24062 2.5 1 21297 2765 49 37933 2.5 1 34964 2969 50 44225 2.5 1 40387 3838 51 39662 2.5 1 36277 3385 52 40239 2.5 1 36283 3956 53 41781 2.5 1 37043 4738 54 37932 2.5 1 34513 3419 55 32864 2.5 1 30393 2471 56 33417 2.5 1 30602 2815 57 36063 2.5 1 33052 3011 58 30135 2.5 1 27493 2642 59 33863 2.5 1 31111 2752 60 32888 2.5 1 30153 2735 61 26002 2.5 1 23558 2444 62 26700 2.5 1 25031 1669 63 21315 2.5 1 19589 1726 64 26428 2.5 1 24395 2033 65 26518 2.5 1 24864 1654 66 21109 2.5 1 19467 1642 67 15735 2.5 1 14382 1353 68 22911 2.5 1 21262 1649 69 22503 2.5 1 20868 1635 70 28097 2.5 1 26068 2029 71 19452 2.5 1 17817 1635 72 28711 2.5 1 26479 2232 73 2285 2.5 1 1871 414 74 2603 2.5 1 2366 237 75 6918 2.5 1 6481 437 76 9519 2.5 1 8851 668 77 9755 2.5 1 9099 656 78 9882 2.5 1 9197 685 79 9112 2.5 1 8482 630 80 8553 2.5 1 7912 641 81 7795 2.5 1 7200 595 82 7202 2.5 1 6648 554 83 6440 2.5 1 5943 497 84 5904 2.5 1 5451 453 85 5218 2.5 1 4799 419 86 4656 2.5 1 4250 406 87 4174 2.5 1 3819 355 88 3567 2.5 1 3227 340 89 3291 2.5 1 2967 324 90 2918 2.5 1 2639 279 91 2525 2.5 1 2252 273 92 2182 2.5 1 1934 248 93 1882 2.5 1 1666 216 94 1655 2.5 1 1425 230 95 1380 2.5 1 1196 184 96 1159 2.5 1 975 184 97 991 2.5 1 803 188 98 840 2.5 1 689 151 99 739 2.5 1 594 145 100 726 2.5 1 572 154 101 656 2.5 1 512 144 102 540 2.5 1 409 131 103 497 2.5 1 366 131 104 425 2.5 1 303 122 105 382 2.5 1 283 99 106 364 2.5 1 247 117 107 352 2.5 1 240 112 108 324 2.5 1 225 99 109 275 2.5 1 182 93 110 270 2.5 1 178 92 111 268 2.5 1 173 95 112 283 2.5 1 193 90 113 221 2.5 1 153 68 114 230 2.5 1 158 72 115 224 2.5 1 155 69 116 244 2.5 1 156 88 117 207 2.5 1 129 78 118 196 2.5 1 124 72 119 201 2.5 1 136 65 120 193 2.5 1 130 63 121 164 2.5 1 111 53 122 211 2.5 1 136 75 123 165 2.5 1 102 63 124 179 2.5 1 125 54 125 159 2.5 1 114 45 126 160 2.5 1 113 47 127 126 2.5 1 101 25 128 133 2.5 1 101 32 129 121 2.5 1 87 34 130 116 2.5 1 94 22 131 83 2.5 1 53 30 132 113 2.5 1 84 29 133 87 2.5 1 60 27 134 64 2.5 1 46 18 135 65 2.5 1 52 13 136 62 2.5 1 46 16 137 32 2.5 1 21 11 138 36 2.5 1 31 5 139 42 2.5 1 26 16 140 33 2.5 1 17 16 141 21 2.5 1 16 5 142 25 2.5 1 10 15 143 19 2.5 1 11 8 144 30 2.5 1 18 12 145 21 2.5 1 10 11 146 26 2.5 1 15 11 147 21 2.5 1 7 14 148 33 2.5 1 17 16 149 59 2.5 1 14 45 150 881 2.5 1 9 872 RUN STATISTICS FOR INPUT FILE: ERR13913496_1.fastq.gz ============================================= 168235405 sequences processed in total