SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913495_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 23461). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913495_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2543.052 s (20.250 µs/read; 2.96 M reads/minute). === Summary === Total reads processed: 125,580,879 Reads with adapters: 51,238,093 (40.8%) Reads written (passing filters): 125,580,879 (100.0%) Total basepairs processed: 18,837,131,850 bp Quality-trimmed: 26,461,326 bp (0.1%) Total written (filtered): 18,642,009,694 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 51238093 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.4% C: 0.2% G: 14.0% T: 48.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 38674902 31395219.8 0 38674902 2 5827426 7848804.9 0 5827426 3 2281049 1962201.2 0 2281049 4 963198 490550.3 0 963198 5 102995 122637.6 0 102995 6 74643 30659.4 0 74643 7 79276 7664.8 0 79276 8 84246 1916.2 0 84246 9 83427 479.1 0 82307 1120 10 83940 119.8 1 75206 8734 11 91134 29.9 1 78034 13100 12 85745 7.5 1 76150 9595 13 77198 1.9 1 67075 10123 14 81292 1.9 1 69188 12104 15 78247 1.9 1 67212 11035 16 80838 1.9 1 67766 13072 17 85689 1.9 1 72805 12884 18 77471 1.9 1 68311 9160 19 66539 1.9 1 57856 8683 20 74593 1.9 1 64555 10038 21 71059 1.9 1 61810 9249 22 67701 1.9 1 59547 8154 23 69587 1.9 1 60388 9199 24 70132 1.9 1 59261 10871 25 66254 1.9 1 57884 8370 26 63591 1.9 1 55854 7737 27 66637 1.9 1 57958 8679 28 61261 1.9 1 54505 6756 29 66529 1.9 1 57303 9226 30 62728 1.9 1 55839 6889 31 53738 1.9 1 47808 5930 32 57298 1.9 1 50994 6304 33 56952 1.9 1 49765 7187 34 56814 1.9 1 49958 6856 35 49486 1.9 1 44360 5126 36 53317 1.9 1 47259 6058 37 49985 1.9 1 44410 5575 38 47884 1.9 1 43381 4503 39 52634 1.9 1 46312 6322 40 43960 1.9 1 39244 4716 41 43259 1.9 1 39246 4013 42 42005 1.9 1 38279 3726 43 48385 1.9 1 43652 4733 44 38615 1.9 1 34400 4215 45 41474 1.9 1 35527 5947 46 36959 1.9 1 32739 4220 47 47523 1.9 1 42777 4746 48 31968 1.9 1 28324 3644 49 33966 1.9 1 30788 3178 50 44046 1.9 1 40300 3746 51 26843 1.9 1 24383 2460 52 40332 1.9 1 35969 4363 53 40115 1.9 1 35755 4360 54 24268 1.9 1 22207 2061 55 19548 1.9 1 18047 1501 56 35593 1.9 1 32614 2979 57 33949 1.9 1 31618 2331 58 20319 1.9 1 18415 1904 59 32692 1.9 1 30537 2155 60 15859 1.9 1 14550 1309 61 23665 1.9 1 21711 1954 62 15506 1.9 1 14574 932 63 8787 1.9 1 7984 803 64 21741 1.9 1 20301 1440 65 20978 1.9 1 19834 1144 66 8737 1.9 1 8027 710 67 10845 1.9 1 9895 950 68 18843 1.9 1 17667 1176 69 11992 1.9 1 11123 869 70 17395 1.9 1 16180 1215 71 15331 1.9 1 14150 1181 72 23724 1.9 1 21903 1821 73 1690 1.9 1 1359 331 74 1477 1.9 1 1299 178 75 4545 1.9 1 4242 303 76 6791 1.9 1 6342 449 77 7395 1.9 1 6917 478 78 7482 1.9 1 7025 457 79 7034 1.9 1 6535 499 80 6775 1.9 1 6259 516 81 6000 1.9 1 5549 451 82 5676 1.9 1 5195 481 83 5068 1.9 1 4659 409 84 4509 1.9 1 4131 378 85 3924 1.9 1 3592 332 86 3454 1.9 1 3167 287 87 3158 1.9 1 2881 277 88 2858 1.9 1 2582 276 89 2548 1.9 1 2266 282 90 2341 1.9 1 2092 249 91 1935 1.9 1 1743 192 92 1688 1.9 1 1490 198 93 1440 1.9 1 1245 195 94 1221 1.9 1 1023 198 95 1120 1.9 1 973 147 96 884 1.9 1 735 149 97 852 1.9 1 703 149 98 706 1.9 1 568 138 99 623 1.9 1 509 114 100 528 1.9 1 404 124 101 499 1.9 1 383 116 102 479 1.9 1 379 100 103 376 1.9 1 273 103 104 327 1.9 1 235 92 105 309 1.9 1 215 94 106 300 1.9 1 205 95 107 255 1.9 1 174 81 108 259 1.9 1 172 87 109 219 1.9 1 146 73 110 240 1.9 1 152 88 111 207 1.9 1 131 76 112 207 1.9 1 143 64 113 192 1.9 1 115 77 114 181 1.9 1 119 62 115 135 1.9 1 91 44 116 166 1.9 1 101 65 117 161 1.9 1 102 59 118 150 1.9 1 95 55 119 159 1.9 1 92 67 120 151 1.9 1 97 54 121 128 1.9 1 91 37 122 130 1.9 1 88 42 123 121 1.9 1 71 50 124 116 1.9 1 77 39 125 99 1.9 1 67 32 126 112 1.9 1 69 43 127 86 1.9 1 51 35 128 84 1.9 1 49 35 129 96 1.9 1 53 43 130 80 1.9 1 60 20 131 60 1.9 1 40 20 132 66 1.9 1 45 21 133 59 1.9 1 36 23 134 60 1.9 1 42 18 135 41 1.9 1 25 16 136 39 1.9 1 29 10 137 31 1.9 1 27 4 138 29 1.9 1 13 16 139 15 1.9 1 9 6 140 19 1.9 1 14 5 141 12 1.9 1 4 8 142 13 1.9 1 7 6 143 26 1.9 1 10 16 144 28 1.9 1 7 21 145 20 1.9 1 5 15 146 15 1.9 1 9 6 147 23 1.9 1 2 21 148 23 1.9 1 4 19 149 80 1.9 1 9 71 150 1031 1.9 1 4 1027 RUN STATISTICS FOR INPUT FILE: ERR13913495_1.fastq.gz ============================================= 125580879 sequences processed in total