SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913494_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 24809). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913494_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 2712.934 s (20.086 µs/read; 2.99 M reads/minute). === Summary === Total reads processed: 135,068,733 Reads with adapters: 69,914,796 (51.8%) Reads written (passing filters): 135,068,733 (100.0%) Total basepairs processed: 20,260,309,950 bp Quality-trimmed: 81,588,467 bp (0.4%) Total written (filtered): 19,989,584,173 bp (98.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 69914796 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 52.5% C: 17.3% G: 0.4% T: 29.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 65476138 33767183.2 0 65476138 2 174359 8441795.8 0 174359 3 165952 2110449.0 0 165952 4 124043 527612.2 0 124043 5 116044 131903.1 0 116044 6 94569 32975.8 0 94569 7 101466 8243.9 0 101466 8 105624 2061.0 0 105624 9 87501 515.2 0 87465 36 10 98896 128.8 1 93034 5862 11 99096 32.2 1 91543 7553 12 93815 8.1 1 67572 26243 13 92113 2.0 1 65019 27094 14 96100 2.0 1 68697 27403 15 83751 2.0 1 59937 23814 16 88869 2.0 1 63142 25727 17 107058 2.0 1 76850 30208 18 64880 2.0 1 46425 18455 19 111104 2.0 1 81731 29373 20 73223 2.0 1 52064 21159 21 67581 2.0 1 48679 18902 22 78656 2.0 1 57129 21527 23 76242 2.0 1 55898 20344 24 94169 2.0 1 69361 24808 25 85063 2.0 1 61554 23509 26 57390 2.0 1 41952 15438 27 65215 2.0 1 47580 17635 28 72462 2.0 1 54043 18419 29 67496 2.0 1 49563 17933 30 71874 2.0 1 53577 18297 31 64797 2.0 1 48185 16612 32 67682 2.0 1 50565 17117 33 68201 2.0 1 50996 17205 34 58899 2.0 1 43974 14925 35 60714 2.0 1 45090 15624 36 55431 2.0 1 41651 13780 37 56203 2.0 1 42446 13757 38 54524 2.0 1 41154 13370 39 59523 2.0 1 45339 14184 40 48231 2.0 1 36246 11985 41 55714 2.0 1 42941 12773 42 50520 2.0 1 38051 12469 43 41482 2.0 1 31348 10134 44 46142 2.0 1 35528 10614 45 43068 2.0 1 32998 10070 46 43462 2.0 1 33343 10119 47 42985 2.0 1 32847 10138 48 44458 2.0 1 34165 10293 49 37606 2.0 1 29417 8189 50 37202 2.0 1 28530 8672 51 39459 2.0 1 30481 8978 52 34628 2.0 1 26899 7729 53 34324 2.0 1 26629 7695 54 32879 2.0 1 25552 7327 55 30145 2.0 1 23860 6285 56 32573 2.0 1 25827 6746 57 26792 2.0 1 20964 5828 58 28318 2.0 1 22224 6094 59 35606 2.0 1 28499 7107 60 19849 2.0 1 15340 4509 61 23841 2.0 1 18863 4978 62 26636 2.0 1 21413 5223 63 20740 2.0 1 16400 4340 64 20595 2.0 1 16425 4170 65 39045 2.0 1 31657 7388 66 12974 2.0 1 9992 2982 67 12483 2.0 1 9707 2776 68 13896 2.0 1 11150 2746 69 18397 2.0 1 14894 3503 70 16866 2.0 1 13410 3456 71 13004 2.0 1 10435 2569 72 15222 2.0 1 12377 2845 73 13088 2.0 1 10465 2623 74 12658 2.0 1 10176 2482 75 17915 2.0 1 14476 3439 76 11066 2.0 1 8794 2272 77 7507 2.0 1 5966 1541 78 3460 2.0 1 2709 751 79 5153 2.0 1 4171 982 80 6003 2.0 1 4980 1023 81 5833 2.0 1 4794 1039 82 5411 2.0 1 4386 1025 83 5118 2.0 1 4143 975 84 4645 2.0 1 3756 889 85 4174 2.0 1 3323 851 86 3633 2.0 1 2926 707 87 3416 2.0 1 2695 721 88 3075 2.0 1 2437 638 89 2649 2.0 1 2057 592 90 2350 2.0 1 1825 525 91 2012 2.0 1 1520 492 92 1770 2.0 1 1346 424 93 1535 2.0 1 1151 384 94 1419 2.0 1 1002 417 95 1168 2.0 1 880 288 96 1034 2.0 1 735 299 97 948 2.0 1 662 286 98 846 2.0 1 590 256 99 749 2.0 1 490 259 100 711 2.0 1 448 263 101 657 2.0 1 413 244 102 632 2.0 1 399 233 103 535 2.0 1 300 235 104 491 2.0 1 277 214 105 453 2.0 1 254 199 106 419 2.0 1 239 180 107 377 2.0 1 209 168 108 362 2.0 1 196 166 109 338 2.0 1 180 158 110 346 2.0 1 180 166 111 329 2.0 1 175 154 112 284 2.0 1 148 136 113 273 2.0 1 154 119 114 306 2.0 1 166 140 115 284 2.0 1 149 135 116 253 2.0 1 154 99 117 235 2.0 1 121 114 118 245 2.0 1 137 108 119 232 2.0 1 108 124 120 224 2.0 1 117 107 121 224 2.0 1 127 97 122 213 2.0 1 108 105 123 219 2.0 1 119 100 124 209 2.0 1 110 99 125 185 2.0 1 104 81 126 176 2.0 1 101 75 127 148 2.0 1 72 76 128 162 2.0 1 102 60 129 126 2.0 1 76 50 130 111 2.0 1 73 38 131 82 2.0 1 56 26 132 71 2.0 1 43 28 133 69 2.0 1 41 28 134 57 2.0 1 40 17 135 55 2.0 1 30 25 136 39 2.0 1 24 15 137 21 2.0 1 14 7 138 20 2.0 1 12 8 139 17 2.0 1 13 4 140 8 2.0 1 5 3 141 9 2.0 1 5 4 142 14 2.0 1 7 7 143 9 2.0 1 3 6 144 10 2.0 1 8 2 145 9 2.0 1 2 7 146 13 2.0 1 7 6 147 9 2.0 1 4 5 148 6 2.0 1 3 3 149 9 2.0 1 6 3 150 10 2.0 1 3 7 RUN STATISTICS FOR INPUT FILE: ERR13913494_2.fastq.gz ============================================= 135068733 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 135068733 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 27162 (0.02%)