SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913494_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 24809). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913494_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2601.465 s (19.260 µs/read; 3.12 M reads/minute). === Summary === Total reads processed: 135,068,733 Reads with adapters: 55,224,290 (40.9%) Reads written (passing filters): 135,068,733 (100.0%) Total basepairs processed: 20,260,309,950 bp Quality-trimmed: 27,805,781 bp (0.1%) Total written (filtered): 20,047,935,939 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 55224290 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.5% C: 0.1% G: 13.8% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 41682830 33767183.2 0 41682830 2 6226552 8441795.8 0 6226552 3 2433750 2110449.0 0 2433750 4 1028979 527612.2 0 1028979 5 112468 131903.1 0 112468 6 80883 32975.8 0 80883 7 87043 8243.9 0 87043 8 91434 2061.0 0 91434 9 91335 515.2 0 90197 1138 10 92088 128.8 1 82584 9504 11 101121 32.2 1 86556 14565 12 93621 8.1 1 82902 10719 13 85149 2.0 1 74225 10924 14 90134 2.0 1 76722 13412 15 85558 2.0 1 73813 11745 16 89459 2.0 1 75137 14322 17 93666 2.0 1 79882 13784 18 85036 2.0 1 75248 9788 19 74188 2.0 1 64703 9485 20 82233 2.0 1 71310 10923 21 78889 2.0 1 68805 10084 22 75982 2.0 1 66983 8999 23 76742 2.0 1 66836 9906 24 78319 2.0 1 66891 11428 25 73388 2.0 1 64454 8934 26 70794 2.0 1 62152 8642 27 73031 2.0 1 63842 9189 28 68121 2.0 1 60587 7534 29 71739 2.0 1 61741 9998 30 70013 2.0 1 62408 7605 31 60620 2.0 1 54200 6420 32 63883 2.0 1 57077 6806 33 63352 2.0 1 55495 7857 34 66933 2.0 1 58554 8379 35 64280 2.0 1 57957 6323 36 46572 2.0 1 41996 4576 37 55943 2.0 1 50241 5702 38 53252 2.0 1 47520 5732 39 55618 2.0 1 49664 5954 40 49137 2.0 1 44430 4707 41 49689 2.0 1 45187 4502 42 54106 2.0 1 48499 5607 43 42303 2.0 1 38083 4220 44 45758 2.0 1 41535 4223 45 43979 2.0 1 39088 4891 46 46549 2.0 1 41086 5463 47 59730 2.0 1 55116 4614 48 23152 2.0 1 20382 2770 49 33268 2.0 1 30700 2568 50 38260 2.0 1 34870 3390 51 36028 2.0 1 33025 3003 52 35374 2.0 1 31939 3435 53 37655 2.0 1 33349 4306 54 33217 2.0 1 30252 2965 55 27946 2.0 1 25850 2096 56 28412 2.0 1 26115 2297 57 31171 2.0 1 28578 2593 58 26449 2.0 1 24085 2364 59 28836 2.0 1 26556 2280 60 28666 2.0 1 26405 2261 61 22661 2.0 1 20578 2083 62 22026 2.0 1 20654 1372 63 18793 2.0 1 17260 1533 64 23218 2.0 1 21297 1921 65 21570 2.0 1 20269 1301 66 18509 2.0 1 17096 1413 67 13030 2.0 1 11906 1124 68 19349 2.0 1 17975 1374 69 19195 2.0 1 17790 1405 70 23730 2.0 1 21962 1768 71 16417 2.0 1 15009 1408 72 23545 2.0 1 21716 1829 73 1878 2.0 1 1560 318 74 2213 2.0 1 2015 198 75 5997 2.0 1 5610 387 76 7643 2.0 1 7196 447 77 8220 2.0 1 7694 526 78 7981 2.0 1 7466 515 79 7476 2.0 1 6908 568 80 6953 2.0 1 6421 532 81 6428 2.0 1 5960 468 82 5739 2.0 1 5313 426 83 5292 2.0 1 4867 425 84 4721 2.0 1 4331 390 85 4103 2.0 1 3762 341 86 3603 2.0 1 3277 326 87 3244 2.0 1 2977 267 88 2927 2.0 1 2630 297 89 2512 2.0 1 2277 235 90 2251 2.0 1 2016 235 91 1880 2.0 1 1696 184 92 1614 2.0 1 1409 205 93 1384 2.0 1 1215 169 94 1238 2.0 1 1067 171 95 1024 2.0 1 874 150 96 873 2.0 1 728 145 97 765 2.0 1 629 136 98 704 2.0 1 569 135 99 558 2.0 1 445 113 100 544 2.0 1 416 128 101 474 2.0 1 374 100 102 451 2.0 1 344 107 103 367 2.0 1 259 108 104 368 2.0 1 246 122 105 309 2.0 1 211 98 106 290 2.0 1 208 82 107 276 2.0 1 191 85 108 238 2.0 1 155 83 109 224 2.0 1 143 81 110 256 2.0 1 177 79 111 217 2.0 1 138 79 112 194 2.0 1 125 69 113 174 2.0 1 113 61 114 205 2.0 1 137 68 115 205 2.0 1 127 78 116 176 2.0 1 116 60 117 135 2.0 1 90 45 118 161 2.0 1 114 47 119 149 2.0 1 98 51 120 146 2.0 1 99 47 121 148 2.0 1 89 59 122 145 2.0 1 95 50 123 131 2.0 1 84 47 124 132 2.0 1 86 46 125 134 2.0 1 88 46 126 141 2.0 1 89 52 127 102 2.0 1 64 38 128 114 2.0 1 76 38 129 79 2.0 1 61 18 130 91 2.0 1 70 21 131 80 2.0 1 60 20 132 66 2.0 1 41 25 133 50 2.0 1 38 12 134 61 2.0 1 42 19 135 53 2.0 1 38 15 136 40 2.0 1 28 12 137 25 2.0 1 20 5 138 20 2.0 1 14 6 139 26 2.0 1 15 11 140 13 2.0 1 5 8 141 12 2.0 1 7 5 142 16 2.0 1 12 4 143 16 2.0 1 4 12 144 16 2.0 1 9 7 145 14 2.0 1 5 9 146 23 2.0 1 11 12 147 23 2.0 1 4 19 148 20 2.0 1 3 17 149 49 2.0 1 7 42 150 944 2.0 1 4 940 RUN STATISTICS FOR INPUT FILE: ERR13913494_1.fastq.gz ============================================= 135068733 sequences processed in total