SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913493_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 14006). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913493_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2310.638 s (19.904 µs/read; 3.01 M reads/minute). === Summary === Total reads processed: 116,088,097 Reads with adapters: 46,487,924 (40.0%) Reads written (passing filters): 116,088,097 (100.0%) Total basepairs processed: 17,413,214,550 bp Quality-trimmed: 22,587,718 bp (0.1%) Total written (filtered): 17,278,886,713 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 46487924 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.5% C: 0.1% G: 13.6% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 36057275 29022024.2 0 36057275 2 5413444 7255506.1 0 5413444 3 2116160 1813876.5 0 2116160 4 888107 453469.1 0 888107 5 73236 113367.3 0 73236 6 51901 28341.8 0 51901 7 55087 7085.5 0 55087 8 57480 1771.4 0 57480 9 57980 442.8 0 56880 1100 10 57515 110.7 1 51180 6335 11 62576 27.7 1 53474 9102 12 57069 6.9 1 50562 6507 13 52280 1.7 1 45496 6784 14 54397 1.7 1 46385 8012 15 51828 1.7 1 44833 6995 16 53429 1.7 1 44789 8640 17 54986 1.7 1 46691 8295 18 49957 1.7 1 44220 5737 19 42926 1.7 1 37388 5538 20 47089 1.7 1 40885 6204 21 44704 1.7 1 38849 5855 22 42787 1.7 1 37579 5208 23 43303 1.7 1 37729 5574 24 42974 1.7 1 36551 6423 25 40837 1.7 1 35878 4959 26 38825 1.7 1 34168 4657 27 40425 1.7 1 35109 5316 28 36768 1.7 1 32640 4128 29 38670 1.7 1 33409 5261 30 36476 1.7 1 32529 3947 31 31252 1.7 1 27714 3538 32 32761 1.7 1 29199 3562 33 32277 1.7 1 28391 3886 34 33161 1.7 1 29262 3899 35 30829 1.7 1 27861 2968 36 26552 1.7 1 23024 3528 37 27714 1.7 1 24658 3056 38 27339 1.7 1 24515 2824 39 25059 1.7 1 22664 2395 40 27565 1.7 1 24321 3244 41 21960 1.7 1 20056 1904 42 25641 1.7 1 22013 3628 43 20360 1.7 1 18416 1944 44 19872 1.7 1 18081 1791 45 24358 1.7 1 21146 3212 46 18795 1.7 1 16424 2371 47 28832 1.7 1 26187 2645 48 10385 1.7 1 8917 1468 49 15346 1.7 1 13989 1357 50 29608 1.7 1 27038 2570 51 13698 1.7 1 12288 1410 52 16084 1.7 1 14460 1624 53 22138 1.7 1 19859 2279 54 11593 1.7 1 10486 1107 55 5651 1.7 1 5214 437 56 14547 1.7 1 13595 952 57 15835 1.7 1 14837 998 58 7257 1.7 1 6588 669 59 11176 1.7 1 10533 643 60 4067 1.7 1 3688 379 61 8528 1.7 1 7784 744 62 3815 1.7 1 3539 276 63 2133 1.7 1 1887 246 64 7790 1.7 1 7258 532 65 5820 1.7 1 5512 308 66 1861 1.7 1 1647 214 67 3430 1.7 1 3072 358 68 6204 1.7 1 5816 388 69 3173 1.7 1 2879 294 70 5039 1.7 1 4627 412 71 4646 1.7 1 4174 472 72 7010 1.7 1 6416 594 73 755 1.7 1 568 187 74 438 1.7 1 348 90 75 1243 1.7 1 1125 118 76 2018 1.7 1 1851 167 77 2256 1.7 1 2078 178 78 2201 1.7 1 1993 208 79 2181 1.7 1 1980 201 80 2041 1.7 1 1835 206 81 1874 1.7 1 1668 206 82 1676 1.7 1 1469 207 83 1541 1.7 1 1351 190 84 1472 1.7 1 1277 195 85 1312 1.7 1 1135 177 86 1117 1.7 1 980 137 87 1037 1.7 1 884 153 88 911 1.7 1 769 142 89 856 1.7 1 723 133 90 788 1.7 1 648 140 91 659 1.7 1 554 105 92 631 1.7 1 501 130 93 580 1.7 1 462 118 94 515 1.7 1 401 114 95 417 1.7 1 317 100 96 417 1.7 1 296 121 97 401 1.7 1 290 111 98 358 1.7 1 256 102 99 328 1.7 1 230 98 100 317 1.7 1 224 93 101 305 1.7 1 221 84 102 291 1.7 1 209 82 103 248 1.7 1 162 86 104 247 1.7 1 167 80 105 216 1.7 1 155 61 106 202 1.7 1 125 77 107 221 1.7 1 139 82 108 201 1.7 1 127 74 109 210 1.7 1 152 58 110 191 1.7 1 116 75 111 168 1.7 1 112 56 112 144 1.7 1 87 57 113 152 1.7 1 103 49 114 141 1.7 1 96 45 115 146 1.7 1 85 61 116 135 1.7 1 87 48 117 122 1.7 1 78 44 118 121 1.7 1 83 38 119 100 1.7 1 62 38 120 134 1.7 1 92 42 121 120 1.7 1 69 51 122 119 1.7 1 81 38 123 107 1.7 1 67 40 124 97 1.7 1 58 39 125 121 1.7 1 81 40 126 99 1.7 1 67 32 127 111 1.7 1 77 34 128 98 1.7 1 70 28 129 76 1.7 1 46 30 130 83 1.7 1 47 36 131 57 1.7 1 47 10 132 53 1.7 1 36 17 133 52 1.7 1 28 24 134 43 1.7 1 27 16 135 32 1.7 1 18 14 136 40 1.7 1 24 16 137 13 1.7 1 9 4 138 26 1.7 1 13 13 139 26 1.7 1 10 16 140 22 1.7 1 13 9 141 16 1.7 1 4 12 142 16 1.7 1 8 8 143 19 1.7 1 4 15 144 25 1.7 1 4 21 145 26 1.7 1 6 20 146 21 1.7 1 2 19 147 33 1.7 1 11 22 148 33 1.7 1 7 26 149 44 1.7 1 4 40 150 609 1.7 1 4 605 RUN STATISTICS FOR INPUT FILE: ERR13913493_1.fastq.gz ============================================= 116088097 sequences processed in total