SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913492_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 14238). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913492_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 3246.947 s (20.079 µs/read; 2.99 M reads/minute). === Summary === Total reads processed: 161,705,499 Reads with adapters: 64,893,214 (40.1%) Reads written (passing filters): 161,705,499 (100.0%) Total basepairs processed: 24,255,824,850 bp Quality-trimmed: 33,177,435 bp (0.1%) Total written (filtered): 24,064,330,151 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 64893214 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.7% C: 0.1% G: 13.4% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 50452308 40426374.8 0 50452308 2 7521422 10106593.7 0 7521422 3 2914278 2526648.4 0 2914278 4 1203793 631662.1 0 1203793 5 96762 157915.5 0 96762 6 69733 39478.9 0 69733 7 73209 9869.7 0 73209 8 77597 2467.4 0 77597 9 76532 616.9 0 75174 1358 10 77658 154.2 1 68718 8940 11 83339 38.6 1 71166 12173 12 76621 9.6 1 67663 8958 13 69741 2.4 1 60271 9470 14 72449 2.4 1 61186 11263 15 68631 2.4 1 58895 9736 16 71625 2.4 1 59631 11994 17 73993 2.4 1 62731 11262 18 66985 2.4 1 58793 8192 19 58509 2.4 1 50611 7898 20 63309 2.4 1 54316 8993 21 61067 2.4 1 52862 8205 22 57988 2.4 1 50800 7188 23 59554 2.4 1 51315 8239 24 59642 2.4 1 50492 9150 25 55531 2.4 1 48268 7263 26 53844 2.4 1 46950 6894 27 55511 2.4 1 48233 7278 28 49960 2.4 1 44286 5674 29 53261 2.4 1 45772 7489 30 51543 2.4 1 45598 5945 31 44588 2.4 1 39463 5125 32 46025 2.4 1 40985 5040 33 45274 2.4 1 39629 5645 34 43450 2.4 1 38704 4746 35 50887 2.4 1 44711 6176 36 33459 2.4 1 30172 3287 37 41258 2.4 1 35886 5372 38 39314 2.4 1 34844 4470 39 34405 2.4 1 30214 4191 40 35316 2.4 1 31659 3657 41 30715 2.4 1 27836 2879 42 34035 2.4 1 29844 4191 43 31442 2.4 1 28065 3377 44 29863 2.4 1 26948 2915 45 32504 2.4 1 28449 4055 46 27255 2.4 1 24142 3113 47 39889 2.4 1 36192 3697 48 14128 2.4 1 12279 1849 49 21476 2.4 1 19617 1859 50 26217 2.4 1 23724 2493 51 21336 2.4 1 19293 2043 52 21777 2.4 1 19320 2457 53 25147 2.4 1 22069 3078 54 19566 2.4 1 17713 1853 55 14648 2.4 1 13468 1180 56 17317 2.4 1 15799 1518 57 19959 2.4 1 18288 1671 58 14693 2.4 1 13250 1443 59 18631 2.4 1 17212 1419 60 13941 2.4 1 12708 1233 61 14231 2.4 1 12768 1463 62 11236 2.4 1 10483 753 63 8326 2.4 1 7508 818 64 13106 2.4 1 12009 1097 65 12105 2.4 1 11338 767 66 7602 2.4 1 6951 651 67 7013 2.4 1 6320 693 68 10436 2.4 1 9650 786 69 8307 2.4 1 7647 660 70 10480 2.4 1 9602 878 71 8535 2.4 1 7681 854 72 12631 2.4 1 11524 1107 73 975 2.4 1 723 252 74 992 2.4 1 839 153 75 2500 2.4 1 2276 224 76 3477 2.4 1 3165 312 77 3680 2.4 1 3387 293 78 3538 2.4 1 3224 314 79 3413 2.4 1 3064 349 80 3172 2.4 1 2870 302 81 2957 2.4 1 2643 314 82 2587 2.4 1 2300 287 83 2403 2.4 1 2144 259 84 2093 2.4 1 1858 235 85 1865 2.4 1 1632 233 86 1677 2.4 1 1477 200 87 1504 2.4 1 1308 196 88 1298 2.4 1 1106 192 89 1206 2.4 1 1027 179 90 1109 2.4 1 913 196 91 1018 2.4 1 842 176 92 888 2.4 1 720 168 93 810 2.4 1 671 139 94 678 2.4 1 524 154 95 620 2.4 1 471 149 96 597 2.4 1 463 134 97 517 2.4 1 391 126 98 456 2.4 1 334 122 99 475 2.4 1 327 148 100 428 2.4 1 303 125 101 376 2.4 1 266 110 102 364 2.4 1 251 113 103 325 2.4 1 226 99 104 297 2.4 1 188 109 105 264 2.4 1 185 79 106 271 2.4 1 180 91 107 236 2.4 1 143 93 108 231 2.4 1 157 74 109 225 2.4 1 151 74 110 241 2.4 1 157 84 111 202 2.4 1 126 76 112 214 2.4 1 142 72 113 193 2.4 1 128 65 114 190 2.4 1 112 78 115 171 2.4 1 118 53 116 181 2.4 1 118 63 117 167 2.4 1 107 60 118 176 2.4 1 115 61 119 153 2.4 1 105 48 120 163 2.4 1 110 53 121 151 2.4 1 101 50 122 145 2.4 1 102 43 123 132 2.4 1 76 56 124 147 2.4 1 98 49 125 141 2.4 1 96 45 126 117 2.4 1 87 30 127 106 2.4 1 76 30 128 91 2.4 1 64 27 129 122 2.4 1 82 40 130 96 2.4 1 66 30 131 88 2.4 1 59 29 132 69 2.4 1 51 18 133 58 2.4 1 41 17 134 62 2.4 1 49 13 135 36 2.4 1 19 17 136 39 2.4 1 23 16 137 42 2.4 1 32 10 138 38 2.4 1 27 11 139 15 2.4 1 7 8 140 26 2.4 1 19 7 141 20 2.4 1 9 11 142 16 2.4 1 7 9 143 22 2.4 1 7 15 144 21 2.4 1 13 8 145 23 2.4 1 9 14 146 20 2.4 1 8 12 147 26 2.4 1 6 20 148 20 2.4 1 7 13 149 61 2.4 1 8 53 150 773 2.4 1 6 767 RUN STATISTICS FOR INPUT FILE: ERR13913492_1.fastq.gz ============================================= 161705499 sequences processed in total