SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913491_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 14735). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913491_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2516.344 s (21.445 µs/read; 2.80 M reads/minute). === Summary === Total reads processed: 117,338,871 Reads with adapters: 46,932,996 (40.0%) Reads written (passing filters): 117,338,871 (100.0%) Total basepairs processed: 17,600,830,650 bp Quality-trimmed: 16,300,909 bp (0.1%) Total written (filtered): 17,468,830,063 bp (99.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 46932996 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.7% C: 0.1% G: 13.9% T: 48.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 36258634 29334717.8 0 36258634 2 5543324 7333679.4 0 5543324 3 2129136 1833419.9 0 2129136 4 912382 458355.0 0 912382 5 68899 114588.7 0 68899 6 56680 28647.2 0 56680 7 56844 7161.8 0 56844 8 60070 1790.4 0 60070 9 59174 447.6 0 58014 1160 10 59425 111.9 1 53886 5539 11 62173 28.0 1 54888 7285 12 59342 7.0 1 53815 5527 13 52564 1.7 1 47238 5326 14 56358 1.7 1 49562 6796 15 52957 1.7 1 47311 5646 16 54491 1.7 1 47514 6977 17 54458 1.7 1 47686 6772 18 50489 1.7 1 45323 5166 19 47363 1.7 1 42523 4840 20 47677 1.7 1 42706 4971 21 47234 1.7 1 42044 5190 22 44681 1.7 1 40464 4217 23 44713 1.7 1 40193 4520 24 44885 1.7 1 39744 5141 25 41857 1.7 1 37910 3947 26 41554 1.7 1 37664 3890 27 41500 1.7 1 37620 3880 28 37913 1.7 1 34692 3221 29 38460 1.7 1 34452 4008 30 36271 1.7 1 33211 3060 31 33958 1.7 1 31053 2905 32 33590 1.7 1 30768 2822 33 33331 1.7 1 29738 3593 34 33087 1.7 1 30203 2884 35 28874 1.7 1 26755 2119 36 30397 1.7 1 27433 2964 37 29117 1.7 1 26482 2635 38 30647 1.7 1 28191 2456 39 24009 1.7 1 21767 2242 40 24604 1.7 1 22825 1779 41 26313 1.7 1 23805 2508 42 23658 1.7 1 21659 1999 43 21940 1.7 1 20023 1917 44 20912 1.7 1 19352 1560 45 22491 1.7 1 20242 2249 46 19751 1.7 1 18031 1720 47 27972 1.7 1 26114 1858 48 9654 1.7 1 8784 870 49 15180 1.7 1 14195 985 50 17000 1.7 1 15727 1273 51 15203 1.7 1 14099 1104 52 15368 1.7 1 14017 1351 53 15513 1.7 1 14055 1458 54 13812 1.7 1 12715 1097 55 12097 1.7 1 11275 822 56 12164 1.7 1 11266 898 57 12632 1.7 1 11645 987 58 10604 1.7 1 9716 888 59 11536 1.7 1 10748 788 60 10249 1.7 1 9435 814 61 9179 1.7 1 8351 828 62 8467 1.7 1 7949 518 63 8341 1.7 1 7687 654 64 8664 1.7 1 8030 634 65 8123 1.7 1 7615 508 66 6912 1.7 1 6420 492 67 5604 1.7 1 5148 456 68 6616 1.7 1 6167 449 69 6699 1.7 1 6221 478 70 7407 1.7 1 6851 556 71 6547 1.7 1 6003 544 72 11468 1.7 1 10679 789 73 942 1.7 1 789 153 74 552 1.7 1 461 91 75 1876 1.7 1 1738 138 76 2800 1.7 1 2630 170 77 3040 1.7 1 2812 228 78 3012 1.7 1 2789 223 79 2830 1.7 1 2608 222 80 2646 1.7 1 2443 203 81 2309 1.7 1 2116 193 82 2133 1.7 1 1974 159 83 1861 1.7 1 1711 150 84 1754 1.7 1 1591 163 85 1596 1.7 1 1456 140 86 1402 1.7 1 1250 152 87 1190 1.7 1 1060 130 88 1097 1.7 1 968 129 89 1027 1.7 1 917 110 90 893 1.7 1 755 138 91 792 1.7 1 714 78 92 688 1.7 1 595 93 93 638 1.7 1 524 114 94 535 1.7 1 449 86 95 467 1.7 1 386 81 96 401 1.7 1 333 68 97 356 1.7 1 289 67 98 320 1.7 1 248 72 99 303 1.7 1 229 74 100 247 1.7 1 202 45 101 185 1.7 1 132 53 102 223 1.7 1 160 63 103 215 1.7 1 142 73 104 183 1.7 1 122 61 105 149 1.7 1 97 52 106 146 1.7 1 97 49 107 133 1.7 1 92 41 108 130 1.7 1 86 44 109 125 1.7 1 86 39 110 116 1.7 1 77 39 111 96 1.7 1 65 31 112 122 1.7 1 86 36 113 69 1.7 1 46 23 114 96 1.7 1 56 40 115 95 1.7 1 59 36 116 88 1.7 1 59 29 117 84 1.7 1 49 35 118 99 1.7 1 67 32 119 77 1.7 1 61 16 120 78 1.7 1 47 31 121 74 1.7 1 46 28 122 71 1.7 1 49 22 123 54 1.7 1 33 21 124 69 1.7 1 49 20 125 74 1.7 1 45 29 126 50 1.7 1 32 18 127 53 1.7 1 34 19 128 45 1.7 1 27 18 129 56 1.7 1 29 27 130 43 1.7 1 27 16 131 26 1.7 1 15 11 132 35 1.7 1 24 11 133 35 1.7 1 12 23 134 29 1.7 1 15 14 135 23 1.7 1 13 10 136 20 1.7 1 12 8 137 19 1.7 1 13 6 138 11 1.7 1 5 6 139 24 1.7 1 9 15 140 24 1.7 1 7 17 141 15 1.7 1 2 13 142 9 1.7 1 5 4 143 18 1.7 1 0 18 144 13 1.7 1 3 10 145 17 1.7 1 3 14 146 15 1.7 1 2 13 147 15 1.7 1 1 14 148 21 1.7 1 4 17 149 43 1.7 1 3 40 150 581 1.7 1 1 580 RUN STATISTICS FOR INPUT FILE: ERR13913491_1.fastq.gz ============================================= 117338871 sequences processed in total