SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913490_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 20533). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913490_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1813.388 s (18.649 µs/read; 3.22 M reads/minute). === Summary === Total reads processed: 97,236,200 Reads with adapters: 39,508,586 (40.6%) Reads written (passing filters): 97,236,200 (100.0%) Total basepairs processed: 14,585,430,000 bp Quality-trimmed: 13,185,660 bp (0.1%) Total written (filtered): 14,455,107,662 bp (99.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 39508586 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.9% C: 0.1% G: 13.7% T: 48.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 30089804 24309050.0 0 30089804 2 4527475 6077262.5 0 4527475 3 1748891 1519315.6 0 1748891 4 757688 379828.9 0 757688 5 72284 94957.2 0 72284 6 59073 23739.3 0 59073 7 59957 5934.8 0 59957 8 61571 1483.7 0 61571 9 62823 370.9 0 61948 875 10 61762 92.7 1 56262 5500 11 66200 23.2 1 58644 7556 12 63169 5.8 1 57415 5754 13 56867 1.4 1 50994 5873 14 60799 1.4 1 53256 7543 15 57067 1.4 1 50844 6223 16 59062 1.4 1 51166 7896 17 59161 1.4 1 51557 7604 18 54568 1.4 1 48876 5692 19 51230 1.4 1 45866 5364 20 52185 1.4 1 46720 5465 21 52423 1.4 1 46618 5805 22 49267 1.4 1 44578 4689 23 50281 1.4 1 45234 5047 24 50091 1.4 1 44381 5710 25 46421 1.4 1 42021 4400 26 46040 1.4 1 41719 4321 27 46120 1.4 1 41943 4177 28 42566 1.4 1 38969 3597 29 43560 1.4 1 39047 4513 30 41624 1.4 1 38169 3455 31 39420 1.4 1 35995 3425 32 38630 1.4 1 35323 3307 33 38938 1.4 1 35126 3812 34 36632 1.4 1 33548 3084 35 37304 1.4 1 33785 3519 36 36702 1.4 1 33366 3336 37 37953 1.4 1 34983 2970 38 36993 1.4 1 33416 3577 39 27535 1.4 1 25344 2191 40 26759 1.4 1 24522 2237 41 29493 1.4 1 27129 2364 42 28659 1.4 1 26382 2277 43 26243 1.4 1 24190 2053 44 28123 1.4 1 25905 2218 45 24907 1.4 1 22659 2248 46 25987 1.4 1 23608 2379 47 35224 1.4 1 32929 2295 48 12512 1.4 1 11354 1158 49 19145 1.4 1 17956 1189 50 21044 1.4 1 19569 1475 51 20224 1.4 1 18850 1374 52 19718 1.4 1 18164 1554 53 20242 1.4 1 18539 1703 54 18432 1.4 1 17157 1275 55 15969 1.4 1 14945 1024 56 15865 1.4 1 14796 1069 57 16761 1.4 1 15506 1255 58 14623 1.4 1 13476 1147 59 15148 1.4 1 14152 996 60 14802 1.4 1 13752 1050 61 12033 1.4 1 11110 923 62 12374 1.4 1 11656 718 63 10228 1.4 1 9518 710 64 11838 1.4 1 11039 799 65 11237 1.4 1 10646 591 66 9744 1.4 1 9146 598 67 7415 1.4 1 6901 514 68 9314 1.4 1 8737 577 69 9329 1.4 1 8741 588 70 10110 1.4 1 9488 622 71 8434 1.4 1 7850 584 72 14990 1.4 1 14061 929 73 1175 1.4 1 1007 168 74 801 1.4 1 704 97 75 2845 1.4 1 2681 164 76 4105 1.4 1 3888 217 77 4125 1.4 1 3911 214 78 3948 1.4 1 3750 198 79 3548 1.4 1 3325 223 80 3461 1.4 1 3233 228 81 3042 1.4 1 2857 185 82 2718 1.4 1 2552 166 83 2416 1.4 1 2259 157 84 2168 1.4 1 2028 140 85 1918 1.4 1 1794 124 86 1701 1.4 1 1558 143 87 1504 1.4 1 1374 130 88 1403 1.4 1 1291 112 89 1147 1.4 1 1057 90 90 921 1.4 1 856 65 91 873 1.4 1 776 97 92 750 1.4 1 681 69 93 678 1.4 1 609 69 94 574 1.4 1 519 55 95 483 1.4 1 435 48 96 421 1.4 1 378 43 97 394 1.4 1 337 57 98 316 1.4 1 263 53 99 294 1.4 1 243 51 100 269 1.4 1 222 47 101 220 1.4 1 185 35 102 174 1.4 1 134 40 103 204 1.4 1 165 39 104 167 1.4 1 132 35 105 138 1.4 1 102 36 106 146 1.4 1 103 43 107 126 1.4 1 82 44 108 111 1.4 1 80 31 109 113 1.4 1 90 23 110 110 1.4 1 73 37 111 90 1.4 1 58 32 112 65 1.4 1 47 18 113 83 1.4 1 62 21 114 80 1.4 1 61 19 115 72 1.4 1 50 22 116 91 1.4 1 59 32 117 78 1.4 1 53 25 118 85 1.4 1 57 28 119 76 1.4 1 56 20 120 77 1.4 1 57 20 121 64 1.4 1 46 18 122 70 1.4 1 56 14 123 61 1.4 1 44 17 124 62 1.4 1 46 16 125 55 1.4 1 36 19 126 53 1.4 1 44 9 127 63 1.4 1 43 20 128 56 1.4 1 45 11 129 32 1.4 1 20 12 130 42 1.4 1 31 11 131 21 1.4 1 18 3 132 17 1.4 1 12 5 133 29 1.4 1 25 4 134 33 1.4 1 20 13 135 27 1.4 1 22 5 136 25 1.4 1 23 2 137 22 1.4 1 14 8 138 15 1.4 1 11 4 139 17 1.4 1 10 7 140 19 1.4 1 13 6 141 11 1.4 1 6 5 142 10 1.4 1 6 4 143 9 1.4 1 6 3 144 11 1.4 1 5 6 145 12 1.4 1 7 5 146 9 1.4 1 3 6 147 14 1.4 1 7 7 148 18 1.4 1 9 9 149 20 1.4 1 4 16 150 323 1.4 1 7 316 RUN STATISTICS FOR INPUT FILE: ERR13913490_1.fastq.gz ============================================= 97236200 sequences processed in total