SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913489_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 14589). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913489_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2252.327 s (20.683 µs/read; 2.90 M reads/minute). === Summary === Total reads processed: 108,898,335 Reads with adapters: 43,788,739 (40.2%) Reads written (passing filters): 108,898,335 (100.0%) Total basepairs processed: 16,334,750,250 bp Quality-trimmed: 21,539,428 bp (0.1%) Total written (filtered): 16,203,613,136 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 43788739 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.7% C: 0.1% G: 13.5% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 33950664 27224583.8 0 33950664 2 5066521 6806145.9 0 5066521 3 1959812 1701536.5 0 1959812 4 820412 425384.1 0 820412 5 68445 106346.0 0 68445 6 49578 26586.5 0 49578 7 52404 6646.6 0 52404 8 55208 1661.7 0 55208 9 55154 415.4 0 54212 942 10 54751 103.9 1 48652 6099 11 60224 26.0 1 51407 8817 12 55732 6.5 1 49442 6290 13 50839 1.6 1 44534 6305 14 52538 1.6 1 44931 7607 15 50027 1.6 1 43414 6613 16 52354 1.6 1 44129 8225 17 53412 1.6 1 45780 7632 18 48788 1.6 1 43415 5373 19 41611 1.6 1 36520 5091 20 46161 1.6 1 40218 5943 21 44363 1.6 1 38743 5620 22 42131 1.6 1 37376 4755 23 42477 1.6 1 37051 5426 24 42540 1.6 1 36320 6220 25 40440 1.6 1 35523 4917 26 38593 1.6 1 34073 4520 27 39164 1.6 1 34358 4806 28 35140 1.6 1 31492 3648 29 38016 1.6 1 32894 5122 30 35413 1.6 1 31728 3685 31 30579 1.6 1 27434 3145 32 32208 1.6 1 28863 3345 33 31784 1.6 1 27953 3831 34 33221 1.6 1 29293 3928 35 28236 1.6 1 25199 3037 36 27767 1.6 1 25151 2616 37 26264 1.6 1 23841 2423 38 29495 1.6 1 25918 3577 39 23160 1.6 1 20732 2428 40 23700 1.6 1 21626 2074 41 22308 1.6 1 20178 2130 42 22589 1.6 1 20402 2187 43 22718 1.6 1 20015 2703 44 19524 1.6 1 17932 1592 45 22540 1.6 1 19778 2762 46 19002 1.6 1 16895 2107 47 26139 1.6 1 24139 2000 48 9931 1.6 1 8751 1180 49 14311 1.6 1 13190 1121 50 16670 1.6 1 15241 1429 51 14185 1.6 1 12937 1248 52 14761 1.6 1 13146 1615 53 16127 1.6 1 14205 1922 54 13312 1.6 1 12190 1122 55 10931 1.6 1 10139 792 56 11790 1.6 1 10810 980 57 12443 1.6 1 11384 1059 58 10416 1.6 1 9460 956 59 11741 1.6 1 10877 864 60 10719 1.6 1 9818 901 61 9128 1.6 1 8246 882 62 8150 1.6 1 7646 504 63 6395 1.6 1 5793 602 64 8864 1.6 1 8150 714 65 8422 1.6 1 7900 522 66 6280 1.6 1 5759 521 67 5148 1.6 1 4635 513 68 7282 1.6 1 6796 486 69 6840 1.6 1 6360 480 70 8584 1.6 1 7910 674 71 6244 1.6 1 5657 587 72 8641 1.6 1 7927 714 73 707 1.6 1 552 155 74 812 1.6 1 704 108 75 2060 1.6 1 1918 142 76 2824 1.6 1 2631 193 77 3086 1.6 1 2874 212 78 3151 1.6 1 2890 261 79 2898 1.6 1 2653 245 80 2696 1.6 1 2438 258 81 2454 1.6 1 2226 228 82 2373 1.6 1 2152 221 83 2038 1.6 1 1845 193 84 1912 1.6 1 1710 202 85 1767 1.6 1 1579 188 86 1670 1.6 1 1486 184 87 1420 1.6 1 1253 167 88 1274 1.6 1 1131 143 89 1134 1.6 1 1009 125 90 1180 1.6 1 1021 159 91 1026 1.6 1 880 146 92 895 1.6 1 753 142 93 789 1.6 1 673 116 94 745 1.6 1 633 112 95 621 1.6 1 505 116 96 568 1.6 1 445 123 97 543 1.6 1 454 89 98 431 1.6 1 331 100 99 422 1.6 1 328 94 100 381 1.6 1 292 89 101 313 1.6 1 223 90 102 294 1.6 1 222 72 103 266 1.6 1 190 76 104 224 1.6 1 154 70 105 254 1.6 1 183 71 106 246 1.6 1 178 68 107 241 1.6 1 143 98 108 200 1.6 1 143 57 109 189 1.6 1 130 59 110 164 1.6 1 120 44 111 157 1.6 1 104 53 112 143 1.6 1 86 57 113 161 1.6 1 108 53 114 160 1.6 1 101 59 115 133 1.6 1 96 37 116 126 1.6 1 78 48 117 123 1.6 1 93 30 118 131 1.6 1 79 52 119 118 1.6 1 73 45 120 142 1.6 1 96 46 121 85 1.6 1 59 26 122 111 1.6 1 68 43 123 112 1.6 1 73 39 124 101 1.6 1 76 25 125 81 1.6 1 51 30 126 78 1.6 1 56 22 127 82 1.6 1 54 28 128 78 1.6 1 55 23 129 62 1.6 1 43 19 130 63 1.6 1 50 13 131 53 1.6 1 33 20 132 47 1.6 1 37 10 133 33 1.6 1 26 7 134 42 1.6 1 28 14 135 27 1.6 1 20 7 136 33 1.6 1 22 11 137 17 1.6 1 9 8 138 14 1.6 1 11 3 139 13 1.6 1 8 5 140 12 1.6 1 7 5 141 18 1.6 1 13 5 142 5 1.6 1 2 3 143 4 1.6 1 1 3 144 11 1.6 1 5 6 145 9 1.6 1 3 6 146 13 1.6 1 3 10 147 15 1.6 1 9 6 148 18 1.6 1 2 16 149 26 1.6 1 3 23 150 353 1.6 1 4 349 RUN STATISTICS FOR INPUT FILE: ERR13913489_1.fastq.gz ============================================= 108898335 sequences processed in total