SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913488_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 16534). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913488_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2343.193 s (20.637 µs/read; 2.91 M reads/minute). === Summary === Total reads processed: 113,542,853 Reads with adapters: 45,835,362 (40.4%) Reads written (passing filters): 113,542,853 (100.0%) Total basepairs processed: 17,031,427,950 bp Quality-trimmed: 23,753,464 bp (0.1%) Total written (filtered): 16,888,415,138 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 45835362 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.1% G: 13.6% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 35357398 28385713.2 0 35357398 2 5281511 7096428.3 0 5281511 3 2050951 1774107.1 0 2050951 4 858406 443526.8 0 858406 5 77125 110881.7 0 77125 6 55791 27720.4 0 55791 7 59366 6930.1 0 59366 8 61703 1732.5 0 61703 9 61815 433.1 0 60911 904 10 62508 108.3 1 55694 6814 11 67471 27.1 1 57814 9657 12 63091 6.8 1 55954 7137 13 57765 1.7 1 51033 6732 14 59467 1.7 1 51117 8350 15 56803 1.7 1 49504 7299 16 58819 1.7 1 49917 8902 17 61271 1.7 1 52773 8498 18 54811 1.7 1 48944 5867 19 47587 1.7 1 41845 5742 20 52032 1.7 1 45640 6392 21 50395 1.7 1 44274 6121 22 48033 1.7 1 42785 5248 23 49013 1.7 1 43154 5859 24 48515 1.7 1 41701 6814 25 45025 1.7 1 39813 5212 26 43135 1.7 1 38371 4764 27 44778 1.7 1 39431 5347 28 42204 1.7 1 37913 4291 29 42382 1.7 1 36797 5585 30 42448 1.7 1 38262 4186 31 33475 1.7 1 30136 3339 32 37018 1.7 1 33380 3638 33 35979 1.7 1 31864 4115 34 34900 1.7 1 31519 3381 35 37398 1.7 1 33212 4186 36 29343 1.7 1 26879 2464 37 34574 1.7 1 30374 4200 38 30223 1.7 1 27524 2699 39 26515 1.7 1 24317 2198 40 29165 1.7 1 26119 3046 41 27575 1.7 1 25173 2402 42 26272 1.7 1 23634 2638 43 26042 1.7 1 23733 2309 44 29054 1.7 1 26165 2889 45 26828 1.7 1 22684 4144 46 21834 1.7 1 19227 2607 47 34643 1.7 1 31387 3256 48 15814 1.7 1 13766 2048 49 22719 1.7 1 20529 2190 50 104825 1.7 1 99078 5747 51 23940 1.7 1 21882 2058 52 18325 1.7 1 16816 1509 53 28615 1.7 1 26549 2066 54 12168 1.7 1 11055 1113 55 2172 1.7 1 1853 319 56 16123 1.7 1 15334 789 57 7912 1.7 1 7464 448 58 2627 1.7 1 2301 326 59 4540 1.7 1 4274 266 60 1244 1.7 1 1013 231 61 5142 1.7 1 4734 408 62 1399 1.7 1 1170 229 63 924 1.7 1 748 176 64 6501 1.7 1 6115 386 65 2480 1.7 1 2325 155 66 777 1.7 1 646 131 67 2480 1.7 1 2217 263 68 5957 1.7 1 5623 334 69 1820 1.7 1 1609 211 70 4809 1.7 1 4480 329 71 5156 1.7 1 4740 416 72 8807 1.7 1 8177 630 73 2146 1.7 1 1884 262 74 494 1.7 1 387 107 75 1064 1.7 1 949 115 76 2087 1.7 1 1923 164 77 2983 1.7 1 2796 187 78 3262 1.7 1 3074 188 79 3028 1.7 1 2816 212 80 2905 1.7 1 2688 217 81 2742 1.7 1 2517 225 82 2476 1.7 1 2268 208 83 2203 1.7 1 2006 197 84 1996 1.7 1 1780 216 85 1759 1.7 1 1581 178 86 1629 1.7 1 1449 180 87 1398 1.7 1 1242 156 88 1307 1.7 1 1139 168 89 1067 1.7 1 960 107 90 982 1.7 1 861 121 91 835 1.7 1 712 123 92 777 1.7 1 654 123 93 708 1.7 1 596 112 94 675 1.7 1 566 109 95 500 1.7 1 417 83 96 456 1.7 1 359 97 97 422 1.7 1 331 91 98 378 1.7 1 290 88 99 314 1.7 1 249 65 100 324 1.7 1 252 72 101 289 1.7 1 211 78 102 264 1.7 1 186 78 103 225 1.7 1 162 63 104 217 1.7 1 155 62 105 200 1.7 1 128 72 106 183 1.7 1 121 62 107 160 1.7 1 97 63 108 190 1.7 1 118 72 109 153 1.7 1 100 53 110 124 1.7 1 82 42 111 152 1.7 1 102 50 112 138 1.7 1 92 46 113 128 1.7 1 89 39 114 127 1.7 1 84 43 115 102 1.7 1 57 45 116 102 1.7 1 69 33 117 135 1.7 1 88 47 118 114 1.7 1 82 32 119 98 1.7 1 67 31 120 93 1.7 1 59 34 121 98 1.7 1 70 28 122 106 1.7 1 68 38 123 82 1.7 1 56 26 124 77 1.7 1 48 29 125 72 1.7 1 48 24 126 77 1.7 1 49 28 127 73 1.7 1 46 27 128 77 1.7 1 61 16 129 49 1.7 1 33 16 130 59 1.7 1 41 18 131 44 1.7 1 26 18 132 40 1.7 1 32 8 133 34 1.7 1 21 13 134 28 1.7 1 20 8 135 16 1.7 1 11 5 136 22 1.7 1 16 6 137 24 1.7 1 13 11 138 11 1.7 1 8 3 139 9 1.7 1 4 5 140 7 1.7 1 5 2 141 6 1.7 1 3 3 142 8 1.7 1 5 3 143 6 1.7 1 2 4 144 7 1.7 1 2 5 145 6 1.7 1 3 3 146 9 1.7 1 4 5 147 14 1.7 1 6 8 148 8 1.7 1 3 5 149 26 1.7 1 4 22 150 423 1.7 1 5 418 RUN STATISTICS FOR INPUT FILE: ERR13913488_1.fastq.gz ============================================= 113542853 sequences processed in total