SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913487_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 15054). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913487_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2523.784 s (21.389 µs/read; 2.81 M reads/minute). === Summary === Total reads processed: 117,994,476 Reads with adapters: 47,378,124 (40.2%) Reads written (passing filters): 117,994,476 (100.0%) Total basepairs processed: 17,699,171,400 bp Quality-trimmed: 24,324,645 bp (0.1%) Total written (filtered): 17,556,782,201 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 47378124 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.7% C: 0.2% G: 13.6% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 36763961 29498619.0 0 36763961 2 5531139 7374654.8 0 5531139 3 2126750 1843663.7 0 2126750 4 877826 460915.9 0 877826 5 67892 115229.0 0 67892 6 48533 28807.2 0 48533 7 51218 7201.8 0 51218 8 54185 1800.5 0 54185 9 54019 450.1 0 52972 1047 10 54880 112.5 1 48491 6389 11 59809 28.1 1 50714 9095 12 55568 7.0 1 49188 6380 13 50244 1.8 1 43838 6406 14 52933 1.8 1 45113 7820 15 50883 1.8 1 43790 7093 16 51334 1.8 1 43071 8263 17 54563 1.8 1 46558 8005 18 49044 1.8 1 43467 5577 19 42117 1.8 1 36794 5323 20 47169 1.8 1 40778 6391 21 45165 1.8 1 39357 5808 22 43094 1.8 1 37970 5124 23 44033 1.8 1 38296 5737 24 44273 1.8 1 37655 6618 25 41348 1.8 1 36270 5078 26 39593 1.8 1 34931 4662 27 40505 1.8 1 35362 5143 28 37252 1.8 1 33207 4045 29 39278 1.8 1 34031 5247 30 38186 1.8 1 34014 4172 31 31882 1.8 1 28344 3538 32 33956 1.8 1 30310 3646 33 33898 1.8 1 29768 4130 34 31886 1.8 1 28472 3414 35 34261 1.8 1 29481 4780 36 33433 1.8 1 30091 3342 37 25443 1.8 1 22734 2709 38 28892 1.8 1 25285 3607 39 28555 1.8 1 25447 3108 40 25832 1.8 1 23067 2765 41 24442 1.8 1 22238 2204 42 26305 1.8 1 23467 2838 43 22945 1.8 1 20541 2404 44 23923 1.8 1 21631 2292 45 26850 1.8 1 23558 3292 46 19607 1.8 1 17323 2284 47 31926 1.8 1 29219 2707 48 9191 1.8 1 8023 1168 49 15847 1.8 1 14552 1295 50 21102 1.8 1 19227 1875 51 15143 1.8 1 13732 1411 52 17509 1.8 1 15640 1869 53 19400 1.8 1 17173 2227 54 14961 1.8 1 13624 1337 55 11387 1.8 1 10548 839 56 14221 1.8 1 13064 1157 57 16326 1.8 1 15125 1201 58 10795 1.8 1 9773 1022 59 15197 1.8 1 14118 1079 60 10542 1.8 1 9675 867 61 10327 1.8 1 9394 933 62 8899 1.8 1 8367 532 63 5881 1.8 1 5329 552 64 10515 1.8 1 9715 800 65 10233 1.8 1 9682 551 66 5628 1.8 1 5154 474 67 5294 1.8 1 4733 561 68 8618 1.8 1 8026 592 69 6545 1.8 1 6040 505 70 8503 1.8 1 7815 688 71 6954 1.8 1 6318 636 72 10434 1.8 1 9563 871 73 816 1.8 1 614 202 74 744 1.8 1 630 114 75 2024 1.8 1 1841 183 76 2991 1.8 1 2772 219 77 3331 1.8 1 3061 270 78 3205 1.8 1 2945 260 79 3052 1.8 1 2785 267 80 2966 1.8 1 2723 243 81 2524 1.8 1 2282 242 82 2448 1.8 1 2220 228 83 2058 1.8 1 1857 201 84 1975 1.8 1 1774 201 85 1822 1.8 1 1637 185 86 1569 1.8 1 1359 210 87 1403 1.8 1 1241 162 88 1255 1.8 1 1076 179 89 1163 1.8 1 1010 153 90 1054 1.8 1 907 147 91 855 1.8 1 705 150 92 783 1.8 1 637 146 93 745 1.8 1 602 143 94 639 1.8 1 521 118 95 582 1.8 1 484 98 96 533 1.8 1 412 121 97 454 1.8 1 363 91 98 421 1.8 1 321 100 99 362 1.8 1 272 90 100 375 1.8 1 257 118 101 307 1.8 1 216 91 102 298 1.8 1 221 77 103 246 1.8 1 179 67 104 261 1.8 1 167 94 105 238 1.8 1 165 73 106 224 1.8 1 168 56 107 189 1.8 1 134 55 108 207 1.8 1 134 73 109 179 1.8 1 107 72 110 174 1.8 1 115 59 111 182 1.8 1 111 71 112 162 1.8 1 108 54 113 133 1.8 1 93 40 114 160 1.8 1 106 54 115 118 1.8 1 71 47 116 127 1.8 1 82 45 117 113 1.8 1 71 42 118 128 1.8 1 84 44 119 125 1.8 1 76 49 120 128 1.8 1 89 39 121 128 1.8 1 76 52 122 110 1.8 1 72 38 123 110 1.8 1 76 34 124 102 1.8 1 74 28 125 92 1.8 1 63 29 126 86 1.8 1 57 29 127 98 1.8 1 71 27 128 78 1.8 1 53 25 129 71 1.8 1 45 26 130 73 1.8 1 53 20 131 54 1.8 1 44 10 132 53 1.8 1 37 16 133 40 1.8 1 32 8 134 43 1.8 1 31 12 135 29 1.8 1 21 8 136 26 1.8 1 19 7 137 20 1.8 1 15 5 138 26 1.8 1 22 4 139 15 1.8 1 13 2 140 18 1.8 1 14 4 141 16 1.8 1 9 7 142 19 1.8 1 11 8 143 10 1.8 1 3 7 144 10 1.8 1 7 3 145 21 1.8 1 7 14 146 7 1.8 1 2 5 147 16 1.8 1 7 9 148 14 1.8 1 3 11 149 38 1.8 1 4 34 150 571 1.8 1 6 565 RUN STATISTICS FOR INPUT FILE: ERR13913487_1.fastq.gz ============================================= 117994476 sequences processed in total