SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913486_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 28018). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913486_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2365.179 s (20.843 µs/read; 2.88 M reads/minute). === Summary === Total reads processed: 113,478,377 Reads with adapters: 46,833,753 (41.3%) Reads written (passing filters): 113,478,377 (100.0%) Total basepairs processed: 17,021,756,550 bp Quality-trimmed: 24,529,922 bp (0.1%) Total written (filtered): 16,828,457,230 bp (98.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 46833753 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.5% C: 0.2% G: 13.9% T: 48.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 34941574 28369594.2 0 34941574 2 5195860 7092398.6 0 5195860 3 2051051 1773099.6 0 2051051 4 876681 443274.9 0 876681 5 111685 110818.7 0 111685 6 81141 27704.7 0 81141 7 87193 6926.2 0 87193 8 91752 1731.5 0 91752 9 90935 432.9 0 89979 956 10 91131 108.2 1 82030 9101 11 99876 27.1 1 85601 14275 12 92951 6.8 1 82452 10499 13 84245 1.7 1 73718 10527 14 89169 1.7 1 76245 12924 15 86041 1.7 1 74441 11600 16 89218 1.7 1 75060 14158 17 93990 1.7 1 80294 13696 18 85411 1.7 1 75790 9621 19 73475 1.7 1 64060 9415 20 81464 1.7 1 70699 10765 21 77573 1.7 1 67705 9868 22 73925 1.7 1 65484 8441 23 75179 1.7 1 65514 9665 24 77215 1.7 1 65698 11517 25 72454 1.7 1 63654 8800 26 69310 1.7 1 61311 7999 27 72418 1.7 1 63419 8999 28 66656 1.7 1 59598 7058 29 71286 1.7 1 61544 9742 30 68098 1.7 1 60983 7115 31 57940 1.7 1 51731 6209 32 62584 1.7 1 55918 6666 33 61334 1.7 1 54024 7310 34 63683 1.7 1 56314 7369 35 54930 1.7 1 49507 5423 36 53300 1.7 1 48380 4920 37 58298 1.7 1 51579 6719 38 54225 1.7 1 48190 6035 39 50579 1.7 1 45883 4696 40 50402 1.7 1 44974 5428 41 52118 1.7 1 47153 4965 42 43187 1.7 1 39493 3694 43 48803 1.7 1 44089 4714 44 46358 1.7 1 41833 4525 45 48609 1.7 1 41730 6879 46 46131 1.7 1 40590 5541 47 62694 1.7 1 57215 5479 48 27491 1.7 1 24093 3398 49 34348 1.7 1 31587 2761 50 56193 1.7 1 51747 4446 51 33962 1.7 1 30771 3191 52 37584 1.7 1 33859 3725 53 52978 1.7 1 47856 5122 54 27916 1.7 1 25558 2358 55 16244 1.7 1 15104 1140 56 34241 1.7 1 31861 2380 57 38959 1.7 1 36731 2228 58 22891 1.7 1 21068 1823 59 31611 1.7 1 29844 1767 60 14027 1.7 1 12880 1147 61 26171 1.7 1 24229 1942 62 12823 1.7 1 12033 790 63 7687 1.7 1 6954 733 64 22247 1.7 1 20944 1303 65 18301 1.7 1 17453 848 66 7113 1.7 1 6570 543 67 10835 1.7 1 9979 856 68 18390 1.7 1 17388 1002 69 9908 1.7 1 9198 710 70 15273 1.7 1 14256 1017 71 14350 1.7 1 13291 1059 72 22277 1.7 1 20774 1503 73 1899 1.7 1 1547 352 74 1300 1.7 1 1130 170 75 3805 1.7 1 3553 252 76 5891 1.7 1 5549 342 77 6521 1.7 1 6136 385 78 6505 1.7 1 6064 441 79 6180 1.7 1 5760 420 80 5822 1.7 1 5385 437 81 5361 1.7 1 4922 439 82 4795 1.7 1 4461 334 83 4302 1.7 1 3959 343 84 3625 1.7 1 3332 293 85 3226 1.7 1 2925 301 86 2913 1.7 1 2634 279 87 2509 1.7 1 2294 215 88 2288 1.7 1 2056 232 89 2027 1.7 1 1838 189 90 1684 1.7 1 1485 199 91 1563 1.7 1 1410 153 92 1386 1.7 1 1227 159 93 1176 1.7 1 1027 149 94 965 1.7 1 826 139 95 881 1.7 1 740 141 96 752 1.7 1 627 125 97 634 1.7 1 546 88 98 620 1.7 1 506 114 99 513 1.7 1 432 81 100 459 1.7 1 346 113 101 392 1.7 1 298 94 102 393 1.7 1 295 98 103 330 1.7 1 240 90 104 310 1.7 1 227 83 105 247 1.7 1 174 73 106 237 1.7 1 162 75 107 219 1.7 1 164 55 108 210 1.7 1 136 74 109 199 1.7 1 126 73 110 186 1.7 1 132 54 111 160 1.7 1 107 53 112 178 1.7 1 102 76 113 166 1.7 1 108 58 114 155 1.7 1 97 58 115 153 1.7 1 104 49 116 136 1.7 1 95 41 117 114 1.7 1 86 28 118 147 1.7 1 94 53 119 109 1.7 1 71 38 120 135 1.7 1 89 46 121 102 1.7 1 73 29 122 119 1.7 1 86 33 123 103 1.7 1 71 32 124 109 1.7 1 77 32 125 119 1.7 1 84 35 126 103 1.7 1 73 30 127 88 1.7 1 58 30 128 91 1.7 1 62 29 129 62 1.7 1 38 24 130 74 1.7 1 55 19 131 63 1.7 1 50 13 132 68 1.7 1 52 16 133 49 1.7 1 35 14 134 42 1.7 1 29 13 135 34 1.7 1 22 12 136 30 1.7 1 21 9 137 25 1.7 1 19 6 138 21 1.7 1 14 7 139 22 1.7 1 14 8 140 28 1.7 1 21 7 141 19 1.7 1 10 9 142 24 1.7 1 18 6 143 16 1.7 1 8 8 144 16 1.7 1 12 4 145 14 1.7 1 7 7 146 12 1.7 1 7 5 147 14 1.7 1 4 10 148 24 1.7 1 11 13 149 34 1.7 1 4 30 150 498 1.7 1 6 492 RUN STATISTICS FOR INPUT FILE: ERR13913486_1.fastq.gz ============================================= 113478377 sequences processed in total