SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913485_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 20096). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913485_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1962.377 s (20.308 µs/read; 2.95 M reads/minute). === Summary === Total reads processed: 96,630,117 Reads with adapters: 49,392,126 (51.1%) Reads written (passing filters): 96,630,117 (100.0%) Total basepairs processed: 14,494,517,550 bp Quality-trimmed: 68,335,778 bp (0.5%) Total written (filtered): 14,304,624,119 bp (98.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 49392126 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 52.3% C: 17.4% G: 0.4% T: 30.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 46684820 24157529.2 0 46684820 2 128501 6039382.3 0 128501 3 116650 1509845.6 0 116650 4 80503 377461.4 0 80503 5 71990 94365.3 0 71990 6 55774 23591.3 0 55774 7 59974 5897.8 0 59974 8 63862 1474.5 0 63862 9 49770 368.6 0 49746 24 10 59865 92.2 1 55578 4287 11 58146 23.0 1 53122 5024 12 57017 5.8 1 40229 16788 13 54718 1.4 1 38066 16652 14 58634 1.4 1 41204 17430 15 50030 1.4 1 35278 14752 16 52333 1.4 1 36627 15706 17 59090 1.4 1 41665 17425 18 42908 1.4 1 30491 12417 19 60559 1.4 1 43361 17198 20 47194 1.4 1 33258 13936 21 42535 1.4 1 30134 12401 22 47109 1.4 1 33627 13482 23 45678 1.4 1 32680 12998 24 52026 1.4 1 37229 14797 25 54016 1.4 1 38574 15442 26 36953 1.4 1 26663 10290 27 39280 1.4 1 28322 10958 28 43411 1.4 1 31907 11504 29 39388 1.4 1 28314 11074 30 40723 1.4 1 29843 10880 31 39304 1.4 1 28935 10369 32 38268 1.4 1 28178 10090 33 39118 1.4 1 28625 10493 34 36114 1.4 1 26644 9470 35 34718 1.4 1 25562 9156 36 34613 1.4 1 25664 8949 37 35943 1.4 1 26796 9147 38 31870 1.4 1 23810 8060 39 35109 1.4 1 26368 8741 40 30779 1.4 1 22838 7941 41 29351 1.4 1 22139 7212 42 28238 1.4 1 20839 7399 43 28481 1.4 1 21338 7143 44 28395 1.4 1 21552 6843 45 24420 1.4 1 18385 6035 46 25915 1.4 1 19671 6244 47 25271 1.4 1 19248 6023 48 24252 1.4 1 18583 5669 49 23306 1.4 1 17851 5455 50 22445 1.4 1 17014 5431 51 21469 1.4 1 16458 5011 52 20406 1.4 1 15720 4686 53 20360 1.4 1 15702 4658 54 20192 1.4 1 15539 4653 55 18298 1.4 1 14145 4153 56 19296 1.4 1 15090 4206 57 16138 1.4 1 12530 3608 58 17381 1.4 1 13435 3946 59 21076 1.4 1 16602 4474 60 12122 1.4 1 9247 2875 61 14245 1.4 1 11118 3127 62 15517 1.4 1 12221 3296 63 13220 1.4 1 10346 2874 64 12432 1.4 1 9791 2641 65 21965 1.4 1 17640 4325 66 9116 1.4 1 6996 2120 67 8555 1.4 1 6698 1857 68 8359 1.4 1 6590 1769 69 11249 1.4 1 9009 2240 70 11079 1.4 1 8692 2387 71 8251 1.4 1 6608 1643 72 9158 1.4 1 7311 1847 73 8695 1.4 1 6886 1809 74 8238 1.4 1 6577 1661 75 11724 1.4 1 9334 2390 76 6708 1.4 1 5329 1379 77 4803 1.4 1 3800 1003 78 2239 1.4 1 1765 474 79 3249 1.4 1 2609 640 80 3906 1.4 1 3168 738 81 3753 1.4 1 3080 673 82 3711 1.4 1 3026 685 83 3375 1.4 1 2671 704 84 3120 1.4 1 2490 630 85 2877 1.4 1 2290 587 86 2538 1.4 1 2027 511 87 2331 1.4 1 1810 521 88 2108 1.4 1 1652 456 89 1799 1.4 1 1383 416 90 1629 1.4 1 1238 391 91 1393 1.4 1 1044 349 92 1375 1.4 1 1037 338 93 1175 1.4 1 863 312 94 966 1.4 1 696 270 95 944 1.4 1 670 274 96 795 1.4 1 563 232 97 723 1.4 1 511 212 98 645 1.4 1 441 204 99 558 1.4 1 350 208 100 543 1.4 1 322 221 101 448 1.4 1 268 180 102 449 1.4 1 277 172 103 353 1.4 1 195 158 104 364 1.4 1 204 160 105 318 1.4 1 186 132 106 311 1.4 1 162 149 107 320 1.4 1 177 143 108 275 1.4 1 134 141 109 270 1.4 1 134 136 110 273 1.4 1 154 119 111 260 1.4 1 144 116 112 222 1.4 1 120 102 113 215 1.4 1 130 85 114 220 1.4 1 121 99 115 180 1.4 1 86 94 116 218 1.4 1 138 80 117 182 1.4 1 94 88 118 188 1.4 1 110 78 119 192 1.4 1 107 85 120 148 1.4 1 86 62 121 158 1.4 1 93 65 122 134 1.4 1 67 67 123 145 1.4 1 87 58 124 132 1.4 1 77 55 125 132 1.4 1 83 49 126 129 1.4 1 74 55 127 98 1.4 1 67 31 128 101 1.4 1 53 48 129 88 1.4 1 57 31 130 80 1.4 1 44 36 131 48 1.4 1 23 25 132 46 1.4 1 34 12 133 38 1.4 1 24 14 134 47 1.4 1 34 13 135 23 1.4 1 14 9 136 31 1.4 1 22 9 137 14 1.4 1 8 6 138 14 1.4 1 11 3 139 18 1.4 1 13 5 140 10 1.4 1 10 141 4 1.4 1 3 1 142 8 1.4 1 6 2 143 6 1.4 1 3 3 144 5 1.4 1 4 1 145 11 1.4 1 6 5 146 7 1.4 1 1 6 147 7 1.4 1 6 1 148 7 1.4 1 3 4 149 7 1.4 1 1 6 150 1 1.4 1 1 RUN STATISTICS FOR INPUT FILE: ERR13913485_2.fastq.gz ============================================= 96630117 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 96630117 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 33250 (0.03%)