SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913485_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 20096). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913485_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1798.294 s (18.610 µs/read; 3.22 M reads/minute). === Summary === Total reads processed: 96,630,117 Reads with adapters: 39,170,600 (40.5%) Reads written (passing filters): 96,630,117 (100.0%) Total basepairs processed: 14,494,517,550 bp Quality-trimmed: 20,144,735 bp (0.1%) Total written (filtered): 14,355,636,201 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 39170600 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.5% C: 0.1% G: 13.9% T: 48.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 29882117 24157529.2 0 29882117 2 4512954 6039382.3 0 4512954 3 1742274 1509845.6 0 1742274 4 723398 377461.4 0 723398 5 67586 94365.3 0 67586 6 48690 23591.3 0 48690 7 51792 5897.8 0 51792 8 54510 1474.5 0 54510 9 54751 368.6 0 53946 805 10 55160 92.2 1 49237 5923 11 60666 23.0 1 51798 8868 12 56924 5.8 1 50499 6425 13 51681 1.4 1 45062 6619 14 54056 1.4 1 46018 8038 15 51708 1.4 1 44566 7142 16 53346 1.4 1 44804 8542 17 55761 1.4 1 47561 8200 18 50859 1.4 1 44981 5878 19 44129 1.4 1 38373 5756 20 49175 1.4 1 42725 6450 21 47344 1.4 1 41372 5972 22 45811 1.4 1 40317 5494 23 46458 1.4 1 40384 6074 24 47136 1.4 1 40063 7073 25 44227 1.4 1 38818 5409 26 42798 1.4 1 37732 5066 27 43937 1.4 1 38439 5498 28 40360 1.4 1 35910 4450 29 42279 1.4 1 36548 5731 30 40739 1.4 1 36302 4437 31 35609 1.4 1 31880 3729 32 37722 1.4 1 33588 4134 33 36744 1.4 1 32361 4383 34 35999 1.4 1 32176 3823 35 37327 1.4 1 33206 4121 36 36697 1.4 1 31647 5050 37 33204 1.4 1 29865 3339 38 32386 1.4 1 29268 3118 39 28256 1.4 1 25295 2961 40 30782 1.4 1 27672 3110 41 30824 1.4 1 27826 2998 42 27457 1.4 1 24780 2677 43 27690 1.4 1 24614 3076 44 26857 1.4 1 24241 2616 45 28937 1.4 1 25890 3047 46 26254 1.4 1 22891 3363 47 36110 1.4 1 33237 2873 48 13223 1.4 1 11530 1693 49 18950 1.4 1 17544 1406 50 23314 1.4 1 21237 2077 51 20344 1.4 1 18580 1764 52 20514 1.4 1 18482 2032 53 22663 1.4 1 20055 2608 54 19947 1.4 1 18184 1763 55 16069 1.4 1 14855 1214 56 17078 1.4 1 15576 1502 57 19221 1.4 1 17535 1686 58 16123 1.4 1 14665 1458 59 17984 1.4 1 16584 1400 60 16603 1.4 1 15205 1398 61 13906 1.4 1 12642 1264 62 13033 1.4 1 12130 903 63 10320 1.4 1 9452 868 64 14306 1.4 1 13173 1133 65 13432 1.4 1 12557 875 66 10427 1.4 1 9659 768 67 8392 1.4 1 7691 701 68 12377 1.4 1 11520 857 69 10887 1.4 1 10115 772 70 13886 1.4 1 12900 986 71 10179 1.4 1 9348 831 72 15002 1.4 1 13843 1159 73 1205 1.4 1 996 209 74 1313 1.4 1 1171 142 75 3629 1.4 1 3362 267 76 4876 1.4 1 4559 317 77 5254 1.4 1 4912 342 78 4972 1.4 1 4629 343 79 4661 1.4 1 4356 305 80 4409 1.4 1 4073 336 81 4084 1.4 1 3741 343 82 3896 1.4 1 3599 297 83 3383 1.4 1 3099 284 84 3142 1.4 1 2881 261 85 2804 1.4 1 2577 227 86 2448 1.4 1 2239 209 87 2175 1.4 1 1985 190 88 1989 1.4 1 1820 169 89 1737 1.4 1 1556 181 90 1536 1.4 1 1389 147 91 1304 1.4 1 1125 179 92 1227 1.4 1 1102 125 93 1067 1.4 1 918 149 94 868 1.4 1 723 145 95 807 1.4 1 682 125 96 709 1.4 1 587 122 97 596 1.4 1 506 90 98 508 1.4 1 410 98 99 420 1.4 1 346 74 100 398 1.4 1 315 83 101 334 1.4 1 245 89 102 313 1.4 1 232 81 103 255 1.4 1 178 77 104 251 1.4 1 174 77 105 216 1.4 1 152 64 106 204 1.4 1 143 61 107 224 1.4 1 161 63 108 185 1.4 1 116 69 109 181 1.4 1 125 56 110 172 1.4 1 112 60 111 168 1.4 1 110 58 112 152 1.4 1 107 45 113 156 1.4 1 93 63 114 142 1.4 1 92 50 115 112 1.4 1 73 39 116 153 1.4 1 97 56 117 110 1.4 1 76 34 118 120 1.4 1 79 41 119 106 1.4 1 75 31 120 105 1.4 1 73 32 121 100 1.4 1 68 32 122 78 1.4 1 54 24 123 105 1.4 1 64 41 124 84 1.4 1 57 27 125 88 1.4 1 58 30 126 89 1.4 1 55 34 127 74 1.4 1 55 19 128 79 1.4 1 54 25 129 66 1.4 1 46 20 130 57 1.4 1 38 19 131 46 1.4 1 33 13 132 35 1.4 1 22 13 133 35 1.4 1 27 8 134 35 1.4 1 27 8 135 22 1.4 1 16 6 136 35 1.4 1 30 5 137 13 1.4 1 12 1 138 22 1.4 1 14 8 139 16 1.4 1 9 7 140 15 1.4 1 11 4 141 9 1.4 1 4 5 142 9 1.4 1 6 3 143 10 1.4 1 4 6 144 9 1.4 1 5 4 145 14 1.4 1 7 7 146 7 1.4 1 2 5 147 13 1.4 1 7 6 148 17 1.4 1 5 12 149 49 1.4 1 5 44 150 632 1.4 1 1 631 RUN STATISTICS FOR INPUT FILE: ERR13913485_1.fastq.gz ============================================= 96630117 sequences processed in total