SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913484_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 24901). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913484_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2309.685 s (21.450 µs/read; 2.80 M reads/minute). === Summary === Total reads processed: 107,677,452 Reads with adapters: 44,158,890 (41.0%) Reads written (passing filters): 107,677,452 (100.0%) Total basepairs processed: 16,151,617,800 bp Quality-trimmed: 21,447,693 bp (0.1%) Total written (filtered): 15,981,243,555 bp (98.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 44158890 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.2% G: 13.6% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 33227902 26919363.0 0 33227902 2 4929749 6729840.8 0 4929749 3 1945111 1682460.2 0 1945111 4 827843 420615.0 0 827843 5 96357 105153.8 0 96357 6 69944 26288.4 0 69944 7 74308 6572.1 0 74308 8 79273 1643.0 0 79273 9 78228 410.8 0 77349 879 10 78689 102.7 1 70729 7960 11 87700 25.7 1 74917 12783 12 81598 6.4 1 72259 9339 13 74034 1.6 1 64908 9126 14 78092 1.6 1 67024 11068 15 74754 1.6 1 64747 10007 16 77410 1.6 1 65447 11963 17 81278 1.6 1 69487 11791 18 73824 1.6 1 65761 8063 19 62972 1.6 1 55197 7775 20 70905 1.6 1 61716 9189 21 68087 1.6 1 59697 8390 22 65529 1.6 1 58174 7355 23 67966 1.6 1 59351 8615 24 66549 1.6 1 56950 9599 25 62767 1.6 1 55346 7421 26 60993 1.6 1 54062 6931 27 62287 1.6 1 54822 7465 28 57367 1.6 1 51329 6038 29 61793 1.6 1 53694 8099 30 58526 1.6 1 52432 6094 31 50658 1.6 1 45526 5132 32 53801 1.6 1 48358 5443 33 53742 1.6 1 47544 6198 34 53838 1.6 1 47842 5996 35 55188 1.6 1 49081 6107 36 44511 1.6 1 40261 4250 37 45800 1.6 1 41649 4151 38 45267 1.6 1 40791 4476 39 43615 1.6 1 39657 3958 40 47729 1.6 1 42870 4859 41 41254 1.6 1 36448 4806 42 37894 1.6 1 35021 2873 43 41135 1.6 1 36671 4464 44 40673 1.6 1 37069 3604 45 41012 1.6 1 36142 4870 46 32262 1.6 1 29073 3189 47 51450 1.6 1 47388 4062 48 17226 1.6 1 15380 1846 49 28522 1.6 1 26450 2072 50 32503 1.6 1 29853 2650 51 27608 1.6 1 25563 2045 52 28786 1.6 1 25942 2844 53 29182 1.6 1 26104 3078 54 26601 1.6 1 24533 2068 55 23387 1.6 1 21715 1672 56 23752 1.6 1 21772 1980 57 25407 1.6 1 23409 1998 58 20200 1.6 1 18622 1578 59 23476 1.6 1 21709 1767 60 22383 1.6 1 20671 1712 61 16187 1.6 1 14670 1517 62 17702 1.6 1 16691 1011 63 13883 1.6 1 12889 994 64 17730 1.6 1 16407 1323 65 17191 1.6 1 16190 1001 66 13874 1.6 1 12966 908 67 9704 1.6 1 8923 781 68 14233 1.6 1 13328 905 69 14172 1.6 1 13299 873 70 17485 1.6 1 16306 1179 71 11476 1.6 1 10630 846 72 16160 1.6 1 14994 1166 73 1375 1.6 1 1155 220 74 1594 1.6 1 1459 135 75 4009 1.6 1 3760 249 76 5333 1.6 1 5015 318 77 5418 1.6 1 5060 358 78 5235 1.6 1 4902 333 79 4866 1.6 1 4520 346 80 4420 1.6 1 4103 317 81 3991 1.6 1 3713 278 82 3592 1.6 1 3314 278 83 3361 1.6 1 3091 270 84 2921 1.6 1 2685 236 85 2487 1.6 1 2296 191 86 2244 1.6 1 2037 207 87 1919 1.6 1 1759 160 88 1727 1.6 1 1553 174 89 1476 1.6 1 1349 127 90 1217 1.6 1 1076 141 91 1055 1.6 1 941 114 92 1000 1.6 1 880 120 93 819 1.6 1 715 104 94 760 1.6 1 654 106 95 587 1.6 1 500 87 96 532 1.6 1 457 75 97 458 1.6 1 387 71 98 446 1.6 1 348 98 99 365 1.6 1 298 67 100 351 1.6 1 276 75 101 285 1.6 1 217 68 102 255 1.6 1 182 73 103 231 1.6 1 175 56 104 181 1.6 1 128 53 105 181 1.6 1 136 45 106 182 1.6 1 123 59 107 175 1.6 1 119 56 108 171 1.6 1 112 59 109 137 1.6 1 96 41 110 151 1.6 1 98 53 111 173 1.6 1 114 59 112 124 1.6 1 87 37 113 125 1.6 1 86 39 114 110 1.6 1 78 32 115 126 1.6 1 77 49 116 100 1.6 1 62 38 117 100 1.6 1 71 29 118 117 1.6 1 80 37 119 96 1.6 1 68 28 120 129 1.6 1 89 40 121 82 1.6 1 57 25 122 90 1.6 1 48 42 123 97 1.6 1 66 31 124 94 1.6 1 62 32 125 89 1.6 1 57 32 126 80 1.6 1 55 25 127 86 1.6 1 67 19 128 61 1.6 1 41 20 129 59 1.6 1 40 19 130 69 1.6 1 47 22 131 44 1.6 1 31 13 132 44 1.6 1 30 14 133 31 1.6 1 21 10 134 32 1.6 1 26 6 135 26 1.6 1 19 7 136 34 1.6 1 27 7 137 18 1.6 1 15 3 138 15 1.6 1 12 3 139 20 1.6 1 15 5 140 11 1.6 1 6 5 141 6 1.6 1 1 5 142 19 1.6 1 10 9 143 17 1.6 1 3 14 144 10 1.6 1 4 6 145 17 1.6 1 5 12 146 10 1.6 1 5 5 147 14 1.6 1 6 8 148 13 1.6 1 8 5 149 34 1.6 1 7 27 150 467 1.6 1 8 459 RUN STATISTICS FOR INPUT FILE: ERR13913484_1.fastq.gz ============================================= 107677452 sequences processed in total