SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913483_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 21228). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913483_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2520.599 s (21.037 µs/read; 2.85 M reads/minute). === Summary === Total reads processed: 119,818,219 Reads with adapters: 48,685,431 (40.6%) Reads written (passing filters): 119,818,219 (100.0%) Total basepairs processed: 17,972,732,850 bp Quality-trimmed: 24,935,456 bp (0.1%) Total written (filtered): 17,798,852,377 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 48685431 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.2% G: 13.7% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 37059177 29954554.8 0 37059177 2 5503590 7488638.7 0 5503590 3 2153740 1872159.7 0 2153740 4 914619 468039.9 0 914619 5 94922 117010.0 0 94922 6 68351 29252.5 0 68351 7 72880 7313.1 0 72880 8 76616 1828.3 0 76616 9 77387 457.1 0 76388 999 10 77179 114.3 1 69115 8064 11 85755 28.6 1 72943 12812 12 79698 7.1 1 70496 9202 13 70582 1.8 1 61282 9300 14 74038 1.8 1 62676 11362 15 70351 1.8 1 60418 9933 16 73235 1.8 1 60954 12281 17 76563 1.8 1 64761 11802 18 70207 1.8 1 61672 8535 19 60365 1.8 1 52494 7871 20 67030 1.8 1 57918 9112 21 64685 1.8 1 56030 8655 22 61926 1.8 1 54288 7638 23 62663 1.8 1 54102 8561 24 62695 1.8 1 52956 9739 25 59045 1.8 1 51554 7491 26 57233 1.8 1 50129 7104 27 58798 1.8 1 51239 7559 28 54260 1.8 1 48101 6159 29 58711 1.8 1 50328 8383 30 57089 1.8 1 50677 6412 31 49795 1.8 1 44304 5491 32 51319 1.8 1 45559 5760 33 50119 1.8 1 43779 6340 34 49573 1.8 1 43752 5821 35 43654 1.8 1 39148 4506 36 44872 1.8 1 40173 4699 37 44196 1.8 1 39261 4935 38 43905 1.8 1 38156 5749 39 40051 1.8 1 36006 4045 40 42771 1.8 1 37274 5497 41 43617 1.8 1 38555 5062 42 34105 1.8 1 30924 3181 43 37663 1.8 1 33831 3832 44 39185 1.8 1 35748 3437 45 30768 1.8 1 26704 4064 46 32851 1.8 1 28986 3865 47 46137 1.8 1 42091 4046 48 16538 1.8 1 14458 2080 49 26163 1.8 1 23883 2280 50 31804 1.8 1 28902 2902 51 25239 1.8 1 22985 2254 52 28510 1.8 1 25426 3084 53 29273 1.8 1 25846 3427 54 23389 1.8 1 21290 2099 55 18667 1.8 1 17200 1467 56 21485 1.8 1 19617 1868 57 24488 1.8 1 22487 2001 58 17855 1.8 1 16145 1710 59 23891 1.8 1 22087 1804 60 18925 1.8 1 17349 1576 61 17019 1.8 1 15424 1595 62 15256 1.8 1 14326 930 63 10551 1.8 1 9641 910 64 16720 1.8 1 15489 1231 65 16140 1.8 1 15167 973 66 10508 1.8 1 9689 819 67 8538 1.8 1 7757 781 68 13584 1.8 1 12685 899 69 11628 1.8 1 10782 846 70 15082 1.8 1 13956 1126 71 11106 1.8 1 10126 980 72 15609 1.8 1 14288 1321 73 1186 1.8 1 957 229 74 1318 1.8 1 1162 156 75 3358 1.8 1 3119 239 76 4781 1.8 1 4434 347 77 5048 1.8 1 4692 356 78 5023 1.8 1 4617 406 79 4605 1.8 1 4257 348 80 4288 1.8 1 3965 323 81 4168 1.8 1 3837 331 82 3735 1.8 1 3411 324 83 3432 1.8 1 3136 296 84 3031 1.8 1 2725 306 85 2556 1.8 1 2333 223 86 2215 1.8 1 1991 224 87 2076 1.8 1 1849 227 88 1751 1.8 1 1568 183 89 1649 1.8 1 1476 173 90 1455 1.8 1 1284 171 91 1232 1.8 1 1068 164 92 1197 1.8 1 1047 150 93 991 1.8 1 862 129 94 827 1.8 1 713 114 95 718 1.8 1 603 115 96 681 1.8 1 569 112 97 587 1.8 1 471 116 98 529 1.8 1 425 104 99 443 1.8 1 347 96 100 469 1.8 1 364 105 101 355 1.8 1 266 89 102 318 1.8 1 237 81 103 270 1.8 1 194 76 104 297 1.8 1 202 95 105 273 1.8 1 208 65 106 221 1.8 1 148 73 107 231 1.8 1 162 69 108 231 1.8 1 155 76 109 203 1.8 1 145 58 110 170 1.8 1 124 46 111 164 1.8 1 106 58 112 159 1.8 1 112 47 113 153 1.8 1 98 55 114 184 1.8 1 125 59 115 126 1.8 1 95 31 116 147 1.8 1 99 48 117 132 1.8 1 85 47 118 142 1.8 1 97 45 119 140 1.8 1 82 58 120 138 1.8 1 98 40 121 117 1.8 1 74 43 122 124 1.8 1 81 43 123 119 1.8 1 90 29 124 91 1.8 1 68 23 125 105 1.8 1 83 22 126 95 1.8 1 69 26 127 93 1.8 1 65 28 128 79 1.8 1 55 24 129 84 1.8 1 58 26 130 69 1.8 1 49 20 131 64 1.8 1 46 18 132 62 1.8 1 50 12 133 39 1.8 1 37 2 134 57 1.8 1 42 15 135 37 1.8 1 28 9 136 38 1.8 1 22 16 137 22 1.8 1 19 3 138 23 1.8 1 15 8 139 13 1.8 1 8 5 140 17 1.8 1 9 8 141 19 1.8 1 10 9 142 16 1.8 1 8 8 143 16 1.8 1 6 10 144 7 1.8 1 4 3 145 13 1.8 1 9 4 146 17 1.8 1 5 12 147 16 1.8 1 6 10 148 29 1.8 1 7 22 149 51 1.8 1 3 48 150 557 1.8 1 8 549 RUN STATISTICS FOR INPUT FILE: ERR13913483_1.fastq.gz ============================================= 119818219 sequences processed in total