SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913482_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 17577). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913482_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 2302.988 s (20.620 µs/read; 2.91 M reads/minute). === Summary === Total reads processed: 111,688,457 Reads with adapters: 57,572,454 (51.5%) Reads written (passing filters): 111,688,457 (100.0%) Total basepairs processed: 16,753,268,550 bp Quality-trimmed: 64,153,244 bp (0.4%) Total written (filtered): 16,562,252,845 bp (98.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 57572454 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 52.7% C: 17.0% G: 0.3% T: 29.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 54732132 27922114.2 0 54732132 2 124675 6980528.6 0 124675 3 118628 1745132.1 0 118628 4 90902 436283.0 0 90902 5 84518 109070.8 0 84518 6 67407 27267.7 0 67407 7 71274 6816.9 0 71274 8 75610 1704.2 0 75610 9 63348 426.1 0 63325 23 10 70120 106.5 1 65558 4562 11 69170 26.6 1 63234 5936 12 67737 6.7 1 46490 21247 13 63430 1.7 1 43572 19858 14 67360 1.7 1 46818 20542 15 58030 1.7 1 40186 17844 16 60127 1.7 1 41643 18484 17 72005 1.7 1 50360 21645 18 45117 1.7 1 31473 13644 19 70892 1.7 1 50433 20459 20 51515 1.7 1 35391 16124 21 47721 1.7 1 33261 14460 22 52533 1.7 1 37192 15341 23 50928 1.7 1 36161 14767 24 56869 1.7 1 40569 16300 25 61920 1.7 1 44096 17824 26 39414 1.7 1 28100 11314 27 41323 1.7 1 29072 12251 28 47824 1.7 1 34763 13061 29 42193 1.7 1 30120 12073 30 45918 1.7 1 33440 12478 31 40790 1.7 1 29474 11316 32 41464 1.7 1 30404 11060 33 40631 1.7 1 29510 11121 34 38748 1.7 1 28063 10685 35 44206 1.7 1 32750 11456 36 36795 1.7 1 26563 10232 37 33084 1.7 1 24521 8563 38 33746 1.7 1 25187 8559 39 32222 1.7 1 23596 8626 40 30491 1.7 1 22766 7725 41 30547 1.7 1 22884 7663 42 28102 1.7 1 20697 7405 43 24886 1.7 1 18567 6319 44 26784 1.7 1 20119 6665 45 25314 1.7 1 18905 6409 46 24443 1.7 1 18454 5989 47 24621 1.7 1 18477 6144 48 24623 1.7 1 18553 6070 49 21498 1.7 1 16380 5118 50 20522 1.7 1 15430 5092 51 21592 1.7 1 16301 5291 52 18582 1.7 1 14295 4287 53 18683 1.7 1 14116 4567 54 17482 1.7 1 13218 4264 55 16157 1.7 1 12510 3647 56 17060 1.7 1 13222 3838 57 13818 1.7 1 10522 3296 58 14624 1.7 1 11284 3340 59 17049 1.7 1 13476 3573 60 11082 1.7 1 8410 2672 61 11718 1.7 1 9025 2693 62 13147 1.7 1 10333 2814 63 10371 1.7 1 8021 2350 64 9760 1.7 1 7580 2180 65 17227 1.7 1 13738 3489 66 6784 1.7 1 5135 1649 67 6142 1.7 1 4788 1354 68 6320 1.7 1 4955 1365 69 8123 1.7 1 6394 1729 70 7940 1.7 1 6109 1831 71 6173 1.7 1 4785 1388 72 6665 1.7 1 5316 1349 73 6090 1.7 1 4777 1313 74 5554 1.7 1 4318 1236 75 7709 1.7 1 6014 1695 76 4455 1.7 1 3435 1020 77 3187 1.7 1 2444 743 78 1339 1.7 1 1004 335 79 1943 1.7 1 1541 402 80 2460 1.7 1 1923 537 81 2380 1.7 1 1907 473 82 2253 1.7 1 1776 477 83 2075 1.7 1 1601 474 84 1916 1.7 1 1479 437 85 1711 1.7 1 1295 416 86 1592 1.7 1 1208 384 87 1363 1.7 1 1006 357 88 1255 1.7 1 923 332 89 1094 1.7 1 783 311 90 1007 1.7 1 702 305 91 904 1.7 1 648 256 92 845 1.7 1 578 267 93 727 1.7 1 482 245 94 714 1.7 1 464 250 95 624 1.7 1 386 238 96 508 1.7 1 328 180 97 488 1.7 1 302 186 98 429 1.7 1 262 167 99 436 1.7 1 245 191 100 400 1.7 1 225 175 101 370 1.7 1 212 158 102 391 1.7 1 213 178 103 321 1.7 1 187 134 104 303 1.7 1 159 144 105 260 1.7 1 144 116 106 270 1.7 1 132 138 107 279 1.7 1 149 130 108 252 1.7 1 128 124 109 226 1.7 1 110 116 110 245 1.7 1 115 130 111 224 1.7 1 120 104 112 214 1.7 1 109 105 113 204 1.7 1 112 92 114 226 1.7 1 115 111 115 178 1.7 1 96 82 116 185 1.7 1 95 90 117 183 1.7 1 93 90 118 177 1.7 1 85 92 119 133 1.7 1 77 56 120 166 1.7 1 92 74 121 144 1.7 1 67 77 122 141 1.7 1 88 53 123 137 1.7 1 72 65 124 120 1.7 1 66 54 125 121 1.7 1 64 57 126 107 1.7 1 71 36 127 93 1.7 1 58 35 128 94 1.7 1 51 43 129 78 1.7 1 47 31 130 81 1.7 1 55 26 131 69 1.7 1 50 19 132 60 1.7 1 45 15 133 24 1.7 1 16 8 134 38 1.7 1 26 12 135 27 1.7 1 20 7 136 39 1.7 1 30 9 137 24 1.7 1 15 9 138 9 1.7 1 6 3 139 15 1.7 1 8 7 140 10 1.7 1 9 1 141 10 1.7 1 5 5 142 10 1.7 1 7 3 143 12 1.7 1 8 4 144 12 1.7 1 10 2 145 14 1.7 1 13 1 146 18 1.7 1 8 10 147 5 1.7 1 4 1 148 8 1.7 1 5 3 149 7 1.7 1 3 4 150 6 1.7 1 2 4 RUN STATISTICS FOR INPUT FILE: ERR13913482_2.fastq.gz ============================================= 111688457 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 111688457 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 16527 (0.01%)