SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913482_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 17577). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913482_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2216.710 s (19.847 µs/read; 3.02 M reads/minute). === Summary === Total reads processed: 111,688,457 Reads with adapters: 45,132,630 (40.4%) Reads written (passing filters): 111,688,457 (100.0%) Total basepairs processed: 16,753,268,550 bp Quality-trimmed: 22,622,749 bp (0.1%) Total written (filtered): 16,607,086,839 bp (99.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 45132630 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.2% G: 13.6% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 34655179 27922114.2 0 34655179 2 5171250 6980528.6 0 5171250 3 2018649 1745132.1 0 2018649 4 849972 436283.0 0 849972 5 82127 109070.8 0 82127 6 59910 27267.7 0 59910 7 63029 6816.9 0 63029 8 68201 1704.2 0 68201 9 63865 426.1 0 62957 908 10 65526 106.5 1 58746 6780 11 71649 26.6 1 61407 10242 12 66833 6.7 1 59509 7324 13 59898 1.7 1 52131 7767 14 62363 1.7 1 53367 8996 15 60654 1.7 1 52201 8453 16 61017 1.7 1 51181 9836 17 64749 1.7 1 55003 9746 18 58703 1.7 1 51909 6794 19 49940 1.7 1 43543 6397 20 55741 1.7 1 48179 7562 21 53320 1.7 1 46484 6836 22 51278 1.7 1 45063 6215 23 52381 1.7 1 45495 6886 24 51087 1.7 1 43493 7594 25 48444 1.7 1 42505 5939 26 46691 1.7 1 41081 5610 27 48131 1.7 1 42139 5992 28 43547 1.7 1 38679 4868 29 46822 1.7 1 40369 6453 30 45575 1.7 1 40615 4960 31 38031 1.7 1 33851 4180 32 40370 1.7 1 36025 4345 33 39980 1.7 1 35075 4905 34 37474 1.7 1 33493 3981 35 39156 1.7 1 34232 4924 36 34401 1.7 1 30911 3490 37 33546 1.7 1 30116 3430 38 36943 1.7 1 32846 4097 39 30792 1.7 1 26746 4046 40 28748 1.7 1 26294 2454 41 31126 1.7 1 27522 3604 42 29682 1.7 1 26862 2820 43 27632 1.7 1 24346 3286 44 24996 1.7 1 22924 2072 45 30560 1.7 1 26621 3939 46 24308 1.7 1 21380 2928 47 35820 1.7 1 32653 3167 48 14259 1.7 1 12501 1758 49 18879 1.7 1 17298 1581 50 25682 1.7 1 23495 2187 51 16368 1.7 1 14897 1471 52 21245 1.7 1 18997 2248 53 21368 1.7 1 18984 2384 54 15445 1.7 1 14110 1335 55 12947 1.7 1 12026 921 56 17526 1.7 1 16049 1477 57 17792 1.7 1 16434 1358 58 13195 1.7 1 11899 1296 59 17770 1.7 1 16632 1138 60 10365 1.7 1 9506 859 61 12791 1.7 1 11653 1138 62 9433 1.7 1 8859 574 63 5587 1.7 1 5062 525 64 11666 1.7 1 10827 839 65 11275 1.7 1 10677 598 66 5290 1.7 1 4862 428 67 5758 1.7 1 5193 565 68 9382 1.7 1 8758 624 69 6451 1.7 1 5955 496 70 9178 1.7 1 8503 675 71 7440 1.7 1 6781 659 72 10239 1.7 1 9398 841 73 767 1.7 1 589 178 74 843 1.7 1 740 103 75 2153 1.7 1 1985 168 76 2970 1.7 1 2721 249 77 3310 1.7 1 3030 280 78 3305 1.7 1 3030 275 79 3031 1.7 1 2796 235 80 2821 1.7 1 2566 255 81 2541 1.7 1 2329 212 82 2295 1.7 1 2086 209 83 2078 1.7 1 1865 213 84 1849 1.7 1 1646 203 85 1625 1.7 1 1428 197 86 1439 1.7 1 1273 166 87 1220 1.7 1 1050 170 88 1109 1.7 1 955 154 89 970 1.7 1 842 128 90 896 1.7 1 771 125 91 779 1.7 1 665 114 92 724 1.7 1 602 122 93 606 1.7 1 514 92 94 589 1.7 1 469 120 95 475 1.7 1 388 87 96 414 1.7 1 340 74 97 366 1.7 1 282 84 98 348 1.7 1 243 105 99 293 1.7 1 220 73 100 274 1.7 1 201 73 101 263 1.7 1 189 74 102 287 1.7 1 211 76 103 226 1.7 1 166 60 104 216 1.7 1 143 73 105 167 1.7 1 100 67 106 203 1.7 1 126 77 107 193 1.7 1 123 70 108 166 1.7 1 98 68 109 158 1.7 1 113 45 110 178 1.7 1 124 54 111 145 1.7 1 95 50 112 154 1.7 1 86 68 113 134 1.7 1 89 45 114 153 1.7 1 100 53 115 139 1.7 1 93 46 116 117 1.7 1 78 39 117 119 1.7 1 80 39 118 142 1.7 1 83 59 119 104 1.7 1 73 31 120 111 1.7 1 75 36 121 98 1.7 1 58 40 122 92 1.7 1 64 28 123 101 1.7 1 69 32 124 109 1.7 1 74 35 125 92 1.7 1 62 30 126 80 1.7 1 53 27 127 69 1.7 1 43 26 128 78 1.7 1 60 18 129 58 1.7 1 38 20 130 67 1.7 1 56 11 131 52 1.7 1 39 13 132 56 1.7 1 46 10 133 31 1.7 1 18 13 134 44 1.7 1 32 12 135 30 1.7 1 25 5 136 43 1.7 1 35 8 137 26 1.7 1 16 10 138 13 1.7 1 10 3 139 15 1.7 1 10 5 140 11 1.7 1 7 4 141 17 1.7 1 7 10 142 12 1.7 1 9 3 143 18 1.7 1 9 9 144 13 1.7 1 9 4 145 20 1.7 1 14 6 146 15 1.7 1 11 4 147 18 1.7 1 6 12 148 12 1.7 1 6 6 149 43 1.7 1 6 37 150 405 1.7 1 3 402 RUN STATISTICS FOR INPUT FILE: ERR13913482_1.fastq.gz ============================================= 111688457 sequences processed in total