SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913481_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 14121). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913481_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1687.409 s (17.332 µs/read; 3.46 M reads/minute). === Summary === Total reads processed: 97,358,675 Reads with adapters: 39,085,573 (40.1%) Reads written (passing filters): 97,358,675 (100.0%) Total basepairs processed: 14,603,801,250 bp Quality-trimmed: 14,135,144 bp (0.1%) Total written (filtered): 14,495,818,714 bp (99.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 39085573 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 38.0% C: 0.1% G: 13.6% T: 48.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 30312543 24339668.8 0 30312543 2 4585706 6084917.2 0 4585706 3 1761772 1521229.3 0 1761772 4 750305 380307.3 0 750305 5 55737 95076.8 0 55737 6 45209 23769.2 0 45209 7 45702 5942.3 0 45702 8 47896 1485.6 0 47896 9 46663 371.4 0 45792 871 10 47359 92.8 1 42718 4641 11 49793 23.2 1 43762 6031 12 47285 5.8 1 42832 4453 13 42170 1.5 1 37696 4474 14 45134 1.5 1 39475 5659 15 42226 1.5 1 37488 4738 16 43536 1.5 1 37793 5743 17 43440 1.5 1 37685 5755 18 40867 1.5 1 36606 4261 19 37556 1.5 1 33528 4028 20 38380 1.5 1 34173 4207 21 37528 1.5 1 33197 4331 22 35759 1.5 1 32202 3557 23 36218 1.5 1 32416 3802 24 35493 1.5 1 31326 4167 25 33233 1.5 1 30048 3185 26 33120 1.5 1 29994 3126 27 32857 1.5 1 29833 3024 28 30252 1.5 1 27552 2700 29 31731 1.5 1 28357 3374 30 29723 1.5 1 27213 2510 31 27589 1.5 1 25212 2377 32 27245 1.5 1 24823 2422 33 27363 1.5 1 24343 3020 34 27448 1.5 1 24782 2666 35 23194 1.5 1 21310 1884 36 24018 1.5 1 21860 2158 37 24251 1.5 1 22082 2169 38 22049 1.5 1 20353 1696 39 21485 1.5 1 19651 1834 40 20675 1.5 1 18680 1995 41 19309 1.5 1 17769 1540 42 19945 1.5 1 17885 2060 43 17621 1.5 1 16344 1277 44 19747 1.5 1 18032 1715 45 17607 1.5 1 15396 2211 46 16059 1.5 1 14509 1550 47 24720 1.5 1 22809 1911 48 9063 1.5 1 8145 918 49 14267 1.5 1 13133 1134 50 41972 1.5 1 39275 2697 51 14100 1.5 1 12981 1119 52 14474 1.5 1 13347 1127 53 21782 1.5 1 20265 1517 54 8194 1.5 1 7519 675 55 2911 1.5 1 2647 264 56 17385 1.5 1 16545 840 57 8893 1.5 1 8385 508 58 3676 1.5 1 3336 340 59 5640 1.5 1 5324 316 60 1309 1.5 1 1145 164 61 6986 1.5 1 6475 511 62 1811 1.5 1 1640 171 63 1102 1.5 1 968 134 64 5870 1.5 1 5535 335 65 3163 1.5 1 2991 172 66 676 1.5 1 593 83 67 2849 1.5 1 2625 224 68 4471 1.5 1 4200 271 69 1909 1.5 1 1742 167 70 4154 1.5 1 3847 307 71 4448 1.5 1 4128 320 72 7734 1.5 1 7252 482 73 2384 1.5 1 2183 201 74 612 1.5 1 520 92 75 665 1.5 1 600 65 76 1607 1.5 1 1497 110 77 2133 1.5 1 2011 122 78 2246 1.5 1 2081 165 79 2040 1.5 1 1898 142 80 1960 1.5 1 1811 149 81 1789 1.5 1 1665 124 82 1568 1.5 1 1455 113 83 1460 1.5 1 1345 115 84 1242 1.5 1 1146 96 85 1117 1.5 1 1002 115 86 1000 1.5 1 917 83 87 880 1.5 1 799 81 88 869 1.5 1 788 81 89 695 1.5 1 624 71 90 604 1.5 1 544 60 91 570 1.5 1 499 71 92 495 1.5 1 437 58 93 420 1.5 1 380 40 94 395 1.5 1 340 55 95 312 1.5 1 268 44 96 306 1.5 1 252 54 97 251 1.5 1 216 35 98 244 1.5 1 193 51 99 186 1.5 1 152 34 100 192 1.5 1 147 45 101 171 1.5 1 135 36 102 131 1.5 1 97 34 103 129 1.5 1 104 25 104 145 1.5 1 102 43 105 108 1.5 1 78 30 106 101 1.5 1 61 40 107 84 1.5 1 51 33 108 94 1.5 1 76 18 109 97 1.5 1 75 22 110 87 1.5 1 60 27 111 64 1.5 1 38 26 112 62 1.5 1 46 16 113 79 1.5 1 59 20 114 74 1.5 1 52 22 115 69 1.5 1 48 21 116 72 1.5 1 46 26 117 69 1.5 1 50 19 118 51 1.5 1 37 14 119 46 1.5 1 32 14 120 69 1.5 1 53 16 121 44 1.5 1 28 16 122 46 1.5 1 32 14 123 53 1.5 1 35 18 124 49 1.5 1 40 9 125 60 1.5 1 48 12 126 45 1.5 1 37 8 127 35 1.5 1 25 10 128 45 1.5 1 32 13 129 23 1.5 1 13 10 130 34 1.5 1 27 7 131 21 1.5 1 12 9 132 33 1.5 1 28 5 133 28 1.5 1 14 14 134 25 1.5 1 15 10 135 21 1.5 1 11 10 136 25 1.5 1 19 6 137 11 1.5 1 7 4 138 20 1.5 1 11 9 139 13 1.5 1 5 8 140 22 1.5 1 9 13 141 16 1.5 1 7 9 142 12 1.5 1 4 8 143 15 1.5 1 6 9 144 17 1.5 1 4 13 145 13 1.5 1 2 11 146 13 1.5 1 5 8 147 6 1.5 1 1 5 148 17 1.5 1 4 13 149 17 1.5 1 5 12 150 393 1.5 1 7 386 RUN STATISTICS FOR INPUT FILE: ERR13913481_1.fastq.gz ============================================= 97358675 sequences processed in total