SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913479_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 10267). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913479_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2128.136 s (21.079 µs/read; 2.85 M reads/minute). === Summary === Total reads processed: 100,960,578 Reads with adapters: 40,156,127 (39.8%) Reads written (passing filters): 100,960,578 (100.0%) Total basepairs processed: 15,144,086,700 bp Quality-trimmed: 13,742,393 bp (0.1%) Total written (filtered): 15,046,196,629 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 40156127 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.9% C: 0.1% G: 13.4% T: 48.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 31447575 25240144.5 0 31447575 2 4797812 6310036.1 0 4797812 3 1829056 1577509.0 0 1829056 4 773337 394377.3 0 773337 5 47455 98594.3 0 47455 6 39208 24648.6 0 39208 7 39028 6162.1 0 39028 8 40838 1540.5 0 40838 9 39955 385.1 0 39076 879 10 40128 96.3 1 35965 4163 11 41210 24.1 1 36235 4975 12 39145 6.0 1 35453 3692 13 34584 1.5 1 31079 3505 14 36704 1.5 1 32222 4482 15 35116 1.5 1 31284 3832 16 35811 1.5 1 31206 4605 17 35711 1.5 1 31172 4539 18 33509 1.5 1 30072 3437 19 30884 1.5 1 27803 3081 20 31073 1.5 1 27873 3200 21 30201 1.5 1 26956 3245 22 28610 1.5 1 25958 2652 23 28781 1.5 1 25829 2952 24 28022 1.5 1 24883 3139 25 26496 1.5 1 23958 2538 26 26096 1.5 1 23654 2442 27 26473 1.5 1 23988 2485 28 24370 1.5 1 22372 1998 29 25284 1.5 1 22708 2576 30 23744 1.5 1 21745 1999 31 21580 1.5 1 19746 1834 32 20854 1.5 1 19070 1784 33 20050 1.5 1 17974 2076 34 19652 1.5 1 17900 1752 35 18447 1.5 1 16897 1550 36 17507 1.5 1 15898 1609 37 17901 1.5 1 16412 1489 38 17773 1.5 1 16265 1508 39 16234 1.5 1 14675 1559 40 15855 1.5 1 14535 1320 41 14551 1.5 1 13304 1247 42 13057 1.5 1 12101 956 43 13906 1.5 1 12772 1134 44 12628 1.5 1 11494 1134 45 12225 1.5 1 10781 1444 46 10939 1.5 1 9937 1002 47 16927 1.5 1 15664 1263 48 5741 1.5 1 5170 571 49 9521 1.5 1 8908 613 50 11730 1.5 1 10846 884 51 7638 1.5 1 6988 650 52 9211 1.5 1 8456 755 53 9279 1.5 1 8465 814 54 6384 1.5 1 5899 485 55 5417 1.5 1 5064 353 56 7796 1.5 1 7228 568 57 7565 1.5 1 7026 539 58 5248 1.5 1 4780 468 59 7598 1.5 1 7182 416 60 3961 1.5 1 3654 307 61 5603 1.5 1 5140 463 62 3641 1.5 1 3429 212 63 2128 1.5 1 1910 218 64 4458 1.5 1 4217 241 65 4421 1.5 1 4169 252 66 1821 1.5 1 1656 165 67 2357 1.5 1 2152 205 68 3427 1.5 1 3190 237 69 2717 1.5 1 2516 201 70 3341 1.5 1 3110 231 71 3260 1.5 1 2987 273 72 4958 1.5 1 4574 384 73 552 1.5 1 443 109 74 236 1.5 1 179 57 75 745 1.5 1 683 62 76 1218 1.5 1 1116 102 77 1342 1.5 1 1233 109 78 1298 1.5 1 1196 102 79 1277 1.5 1 1152 125 80 1236 1.5 1 1113 123 81 1129 1.5 1 1016 113 82 973 1.5 1 876 97 83 867 1.5 1 781 86 84 720 1.5 1 636 84 85 683 1.5 1 614 69 86 609 1.5 1 526 83 87 523 1.5 1 473 50 88 486 1.5 1 426 60 89 481 1.5 1 414 67 90 421 1.5 1 354 67 91 385 1.5 1 323 62 92 343 1.5 1 281 62 93 299 1.5 1 243 56 94 280 1.5 1 221 59 95 257 1.5 1 205 52 96 241 1.5 1 186 55 97 196 1.5 1 159 37 98 221 1.5 1 169 52 99 195 1.5 1 149 46 100 179 1.5 1 133 46 101 174 1.5 1 133 41 102 166 1.5 1 125 41 103 127 1.5 1 87 40 104 150 1.5 1 103 47 105 108 1.5 1 81 27 106 107 1.5 1 80 27 107 114 1.5 1 69 45 108 103 1.5 1 69 34 109 85 1.5 1 59 26 110 99 1.5 1 65 34 111 82 1.5 1 48 34 112 84 1.5 1 59 25 113 98 1.5 1 72 26 114 86 1.5 1 60 26 115 60 1.5 1 38 22 116 81 1.5 1 60 21 117 57 1.5 1 41 16 118 74 1.5 1 53 21 119 55 1.5 1 35 20 120 62 1.5 1 44 18 121 59 1.5 1 38 21 122 64 1.5 1 42 22 123 39 1.5 1 29 10 124 51 1.5 1 31 20 125 49 1.5 1 33 16 126 60 1.5 1 47 13 127 45 1.5 1 31 14 128 41 1.5 1 31 10 129 48 1.5 1 29 19 130 28 1.5 1 20 8 131 30 1.5 1 23 7 132 37 1.5 1 26 11 133 26 1.5 1 14 12 134 18 1.5 1 12 6 135 12 1.5 1 8 4 136 19 1.5 1 11 8 137 10 1.5 1 3 7 138 9 1.5 1 6 3 139 6 1.5 1 5 1 140 7 1.5 1 2 5 141 5 1.5 1 3 2 142 7 1.5 1 2 5 143 12 1.5 1 4 8 144 14 1.5 1 3 11 145 11 1.5 1 1 10 146 19 1.5 1 5 14 147 14 1.5 1 4 10 148 12 1.5 1 2 10 149 23 1.5 1 3 20 150 465 1.5 1 2 463 RUN STATISTICS FOR INPUT FILE: ERR13913479_1.fastq.gz ============================================= 100960578 sequences processed in total