SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913478_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 19699). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913478_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2209.235 s (21.751 µs/read; 2.76 M reads/minute). === Summary === Total reads processed: 101,568,210 Reads with adapters: 41,183,247 (40.5%) Reads written (passing filters): 101,568,210 (100.0%) Total basepairs processed: 15,235,231,500 bp Quality-trimmed: 21,309,513 bp (0.1%) Total written (filtered): 15,091,728,219 bp (99.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 41183247 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.5% C: 0.2% G: 13.8% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 31333144 25392052.5 0 31333144 2 4706599 6348013.1 0 4706599 3 1843181 1587003.3 0 1843181 4 780281 396750.8 0 780281 5 83834 99187.7 0 83834 6 60399 24796.9 0 60399 7 63884 6199.2 0 63884 8 67504 1549.8 0 67504 9 67719 387.5 0 66852 867 10 67426 96.9 1 60347 7079 11 74314 24.2 1 63236 11078 12 67691 6.1 1 59716 7975 13 61174 1.5 1 53208 7966 14 63826 1.5 1 54150 9676 15 60903 1.5 1 52492 8411 16 63107 1.5 1 52733 10374 17 66094 1.5 1 56204 9890 18 59903 1.5 1 52803 7100 19 51887 1.5 1 45244 6643 20 56771 1.5 1 49191 7580 21 54093 1.5 1 47117 6976 22 51239 1.5 1 45000 6239 23 51870 1.5 1 44909 6961 24 52681 1.5 1 44507 8174 25 49422 1.5 1 43161 6261 26 47166 1.5 1 41573 5593 27 48862 1.5 1 42544 6318 28 44810 1.5 1 39962 4848 29 48561 1.5 1 41761 6800 30 46649 1.5 1 41540 5109 31 39588 1.5 1 35207 4381 32 40896 1.5 1 36444 4452 33 40413 1.5 1 35444 4969 34 43164 1.5 1 37664 5500 35 35133 1.5 1 31082 4051 36 36239 1.5 1 32602 3637 37 33787 1.5 1 30235 3552 38 36848 1.5 1 32387 4461 39 34756 1.5 1 31262 3494 40 28459 1.5 1 25373 3086 41 31554 1.5 1 28494 3060 42 28530 1.5 1 25894 2636 43 29765 1.5 1 26743 3022 44 28034 1.5 1 25633 2401 45 25205 1.5 1 22247 2958 46 25857 1.5 1 22792 3065 47 36752 1.5 1 33606 3146 48 12528 1.5 1 10905 1623 49 20245 1.5 1 18562 1683 50 23287 1.5 1 21207 2080 51 19973 1.5 1 18236 1737 52 20592 1.5 1 18559 2033 53 21921 1.5 1 19332 2589 54 18333 1.5 1 16681 1652 55 15271 1.5 1 14134 1137 56 16197 1.5 1 14775 1422 57 17752 1.5 1 16284 1468 58 14236 1.5 1 12875 1361 59 16864 1.5 1 15522 1342 60 15220 1.5 1 13939 1281 61 12920 1.5 1 11658 1262 62 11847 1.5 1 11152 695 63 8828 1.5 1 8043 785 64 12388 1.5 1 11417 971 65 11906 1.5 1 11174 732 66 8566 1.5 1 7950 616 67 6955 1.5 1 6325 630 68 10007 1.5 1 9300 707 69 9183 1.5 1 8516 667 70 11394 1.5 1 10543 851 71 8806 1.5 1 8025 781 72 12927 1.5 1 11870 1057 73 909 1.5 1 716 193 74 1037 1.5 1 919 118 75 2818 1.5 1 2626 192 76 3772 1.5 1 3515 257 77 4115 1.5 1 3827 288 78 3954 1.5 1 3658 296 79 3631 1.5 1 3355 276 80 3493 1.5 1 3217 276 81 3136 1.5 1 2901 235 82 2955 1.5 1 2714 241 83 2671 1.5 1 2422 249 84 2306 1.5 1 2103 203 85 2012 1.5 1 1846 166 86 1960 1.5 1 1770 190 87 1654 1.5 1 1491 163 88 1483 1.5 1 1335 148 89 1310 1.5 1 1185 125 90 1111 1.5 1 980 131 91 1049 1.5 1 938 111 92 939 1.5 1 829 110 93 790 1.5 1 686 104 94 627 1.5 1 544 83 95 555 1.5 1 465 90 96 525 1.5 1 439 86 97 449 1.5 1 370 79 98 396 1.5 1 320 76 99 343 1.5 1 274 69 100 336 1.5 1 260 76 101 321 1.5 1 254 67 102 269 1.5 1 201 68 103 237 1.5 1 172 65 104 200 1.5 1 144 56 105 175 1.5 1 122 53 106 166 1.5 1 119 47 107 159 1.5 1 113 46 108 177 1.5 1 121 56 109 141 1.5 1 86 55 110 129 1.5 1 92 37 111 112 1.5 1 86 26 112 134 1.5 1 84 50 113 113 1.5 1 80 33 114 100 1.5 1 78 22 115 124 1.5 1 81 43 116 126 1.5 1 92 34 117 102 1.5 1 68 34 118 109 1.5 1 75 34 119 98 1.5 1 69 29 120 105 1.5 1 69 36 121 93 1.5 1 57 36 122 89 1.5 1 71 18 123 72 1.5 1 52 20 124 84 1.5 1 56 28 125 96 1.5 1 76 20 126 84 1.5 1 54 30 127 69 1.5 1 51 18 128 74 1.5 1 51 23 129 51 1.5 1 42 9 130 63 1.5 1 51 12 131 52 1.5 1 35 17 132 54 1.5 1 36 18 133 41 1.5 1 34 7 134 47 1.5 1 38 9 135 50 1.5 1 41 9 136 35 1.5 1 26 9 137 23 1.5 1 18 5 138 17 1.5 1 11 6 139 17 1.5 1 12 5 140 15 1.5 1 10 5 141 10 1.5 1 7 3 142 12 1.5 1 4 8 143 13 1.5 1 8 5 144 15 1.5 1 7 8 145 25 1.5 1 16 9 146 15 1.5 1 5 10 147 28 1.5 1 17 11 148 9 1.5 1 6 3 149 27 1.5 1 5 22 150 414 1.5 1 7 407 RUN STATISTICS FOR INPUT FILE: ERR13913478_1.fastq.gz ============================================= 101568210 sequences processed in total