SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913477_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 21031). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913477_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2409.580 s (18.766 µs/read; 3.20 M reads/minute). === Summary === Total reads processed: 128,399,290 Reads with adapters: 52,280,622 (40.7%) Reads written (passing filters): 128,399,290 (100.0%) Total basepairs processed: 19,259,893,500 bp Quality-trimmed: 27,278,400 bp (0.1%) Total written (filtered): 19,073,990,105 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 52280622 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.7% C: 0.2% G: 13.7% T: 48.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 39886347 32099822.5 0 39886347 2 5897732 8024955.6 0 5897732 3 2309868 2006238.9 0 2309868 4 974772 501559.7 0 974772 5 98705 125389.9 0 98705 6 70945 31347.5 0 70945 7 75736 7836.9 0 75736 8 79751 1959.2 0 79751 9 79293 489.8 0 78158 1135 10 79740 122.5 1 71276 8464 11 87282 30.6 1 74552 12730 12 81842 7.7 1 72474 9368 13 73557 1.9 1 63997 9560 14 77715 1.9 1 66352 11363 15 74498 1.9 1 63945 10553 16 76412 1.9 1 64149 12263 17 81346 1.9 1 69072 12274 18 72406 1.9 1 64129 8277 19 62333 1.9 1 54313 8020 20 70327 1.9 1 60708 9619 21 66660 1.9 1 57909 8751 22 64270 1.9 1 56695 7575 23 65378 1.9 1 56864 8514 24 65946 1.9 1 55848 10098 25 61699 1.9 1 54179 7520 26 59167 1.9 1 52268 6899 27 61155 1.9 1 53478 7677 28 55979 1.9 1 49884 6095 29 60045 1.9 1 51743 8302 30 58292 1.9 1 51902 6390 31 49208 1.9 1 43870 5338 32 52187 1.9 1 46529 5658 33 52104 1.9 1 45876 6228 34 49289 1.9 1 44155 5134 35 49615 1.9 1 43663 5952 36 52588 1.9 1 46653 5935 37 47424 1.9 1 42909 4515 38 37936 1.9 1 34431 3505 39 43371 1.9 1 38635 4736 40 44308 1.9 1 38869 5439 41 39302 1.9 1 35779 3523 42 42441 1.9 1 37461 4980 43 40854 1.9 1 36459 4395 44 39482 1.9 1 35564 3918 45 43656 1.9 1 37840 5816 46 30941 1.9 1 27460 3481 47 54481 1.9 1 49424 5057 48 17614 1.9 1 15363 2251 49 30267 1.9 1 27498 2769 50 54192 1.9 1 49711 4481 51 25384 1.9 1 22991 2393 52 36633 1.9 1 32916 3717 53 41050 1.9 1 37000 4050 54 22735 1.9 1 20754 1981 55 13394 1.9 1 12437 957 56 32274 1.9 1 30117 2157 57 33026 1.9 1 31104 1922 58 15348 1.9 1 14019 1329 59 26568 1.9 1 25171 1397 60 9220 1.9 1 8364 856 61 18348 1.9 1 17030 1318 62 10506 1.9 1 9866 640 63 5107 1.9 1 4610 497 64 17912 1.9 1 16857 1055 65 15524 1.9 1 14781 743 66 4820 1.9 1 4402 418 67 7940 1.9 1 7237 703 68 15133 1.9 1 14273 860 69 8330 1.9 1 7717 613 70 12430 1.9 1 11598 832 71 11855 1.9 1 10919 936 72 18290 1.9 1 16933 1357 73 1618 1.9 1 1319 299 74 1124 1.9 1 958 166 75 3088 1.9 1 2849 239 76 5056 1.9 1 4695 361 77 5703 1.9 1 5329 374 78 5815 1.9 1 5438 377 79 5521 1.9 1 5134 387 80 5303 1.9 1 4866 437 81 4650 1.9 1 4307 343 82 4223 1.9 1 3867 356 83 3811 1.9 1 3501 310 84 3447 1.9 1 3175 272 85 2937 1.9 1 2656 281 86 2625 1.9 1 2392 233 87 2355 1.9 1 2130 225 88 2099 1.9 1 1888 211 89 1886 1.9 1 1697 189 90 1672 1.9 1 1475 197 91 1436 1.9 1 1258 178 92 1336 1.9 1 1163 173 93 1091 1.9 1 959 132 94 984 1.9 1 832 152 95 821 1.9 1 693 128 96 756 1.9 1 631 125 97 615 1.9 1 502 113 98 563 1.9 1 462 101 99 538 1.9 1 429 109 100 445 1.9 1 326 119 101 380 1.9 1 275 105 102 374 1.9 1 278 96 103 321 1.9 1 233 88 104 343 1.9 1 232 111 105 275 1.9 1 212 63 106 262 1.9 1 168 94 107 237 1.9 1 155 82 108 233 1.9 1 154 79 109 231 1.9 1 146 85 110 206 1.9 1 134 72 111 179 1.9 1 124 55 112 189 1.9 1 112 77 113 175 1.9 1 107 68 114 161 1.9 1 106 55 115 152 1.9 1 90 62 116 165 1.9 1 114 51 117 162 1.9 1 99 63 118 152 1.9 1 102 50 119 137 1.9 1 93 44 120 125 1.9 1 86 39 121 155 1.9 1 105 50 122 129 1.9 1 94 35 123 111 1.9 1 76 35 124 111 1.9 1 73 38 125 111 1.9 1 74 37 126 131 1.9 1 82 49 127 89 1.9 1 64 25 128 91 1.9 1 67 24 129 77 1.9 1 57 20 130 68 1.9 1 45 23 131 56 1.9 1 34 22 132 76 1.9 1 56 20 133 46 1.9 1 34 12 134 55 1.9 1 36 19 135 29 1.9 1 18 11 136 33 1.9 1 22 11 137 36 1.9 1 26 10 138 19 1.9 1 15 4 139 26 1.9 1 15 11 140 23 1.9 1 14 9 141 11 1.9 1 3 8 142 12 1.9 1 6 6 143 23 1.9 1 8 15 144 17 1.9 1 7 10 145 17 1.9 1 7 10 146 18 1.9 1 13 5 147 15 1.9 1 5 10 148 27 1.9 1 9 18 149 60 1.9 1 6 54 150 688 1.9 1 7 681 RUN STATISTICS FOR INPUT FILE: ERR13913477_1.fastq.gz ============================================= 128399290 sequences processed in total