SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913476_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 23311). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913476_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 3241.604 s (21.405 µs/read; 2.80 M reads/minute). === Summary === Total reads processed: 151,439,032 Reads with adapters: 61,937,022 (40.9%) Reads written (passing filters): 151,439,032 (100.0%) Total basepairs processed: 22,715,854,800 bp Quality-trimmed: 30,669,599 bp (0.1%) Total written (filtered): 22,484,253,870 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 61937022 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.1% G: 13.8% T: 48.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 46840855 37859758.0 0 46840855 2 6937633 9464939.5 0 6937633 3 2725101 2366234.9 0 2725101 4 1163102 591558.7 0 1163102 5 131448 147889.7 0 131448 6 94352 36972.4 0 94352 7 101892 9243.1 0 101892 8 106224 2310.8 0 106224 9 107860 577.7 0 106633 1227 10 107231 144.4 1 96125 11106 11 116580 36.1 1 99474 17106 12 108856 9.0 1 96224 12632 13 98333 2.3 1 85549 12784 14 103155 2.3 1 87453 15702 15 98217 2.3 1 84383 13834 16 103085 2.3 1 86202 16883 17 107985 2.3 1 91755 16230 18 96731 2.3 1 85317 11414 19 84427 2.3 1 73447 10980 20 93532 2.3 1 80971 12561 21 89334 2.3 1 77510 11824 22 85301 2.3 1 74982 10319 23 87116 2.3 1 75645 11471 24 88438 2.3 1 74946 13492 25 82560 2.3 1 72247 10313 26 79082 2.3 1 69564 9518 27 81939 2.3 1 71513 10426 28 74840 2.3 1 66523 8317 29 81215 2.3 1 69907 11308 30 77471 2.3 1 68809 8662 31 67684 2.3 1 60278 7406 32 70093 2.3 1 62391 7702 33 70010 2.3 1 61307 8703 34 72641 2.3 1 63963 8678 35 66748 2.3 1 59823 6925 36 54613 2.3 1 49362 5251 37 67392 2.3 1 58556 8836 38 58323 2.3 1 52566 5757 39 57089 2.3 1 50518 6571 40 56339 2.3 1 50647 5692 41 56089 2.3 1 50011 6078 42 54648 2.3 1 49226 5422 43 48777 2.3 1 44464 4313 44 55651 2.3 1 50748 4903 45 44556 2.3 1 39118 5438 46 50206 2.3 1 44062 6144 47 65572 2.3 1 59976 5596 48 25614 2.3 1 22342 3272 49 37327 2.3 1 34286 3041 50 48047 2.3 1 43768 4279 51 36680 2.3 1 33394 3286 52 39004 2.3 1 34956 4048 53 49182 2.3 1 43735 5447 54 32255 2.3 1 29530 2725 55 23613 2.3 1 21897 1716 56 32775 2.3 1 30151 2624 57 38655 2.3 1 35960 2695 58 26836 2.3 1 24535 2301 59 35275 2.3 1 32954 2321 60 22367 2.3 1 20634 1733 61 28325 2.3 1 25890 2435 62 18404 2.3 1 17335 1069 63 12962 2.3 1 11834 1128 64 24961 2.3 1 23257 1704 65 22734 2.3 1 21506 1228 66 11823 2.3 1 10948 875 67 13061 2.3 1 11896 1165 68 20409 2.3 1 19175 1234 69 13969 2.3 1 12943 1026 70 19010 2.3 1 17690 1320 71 15885 2.3 1 14605 1280 72 24037 2.3 1 22259 1778 73 1726 2.3 1 1380 346 74 1556 2.3 1 1359 197 75 4614 2.3 1 4293 321 76 6652 2.3 1 6220 432 77 7251 2.3 1 6758 493 78 7051 2.3 1 6555 496 79 6690 2.3 1 6199 491 80 6275 2.3 1 5824 451 81 5519 2.3 1 5126 393 82 4961 2.3 1 4536 425 83 4435 2.3 1 4086 349 84 3960 2.3 1 3646 314 85 3372 2.3 1 3036 336 86 3180 2.3 1 2888 292 87 2723 2.3 1 2439 284 88 2422 2.3 1 2175 247 89 2115 2.3 1 1886 229 90 1912 2.3 1 1683 229 91 1572 2.3 1 1386 186 92 1336 2.3 1 1176 160 93 1172 2.3 1 997 175 94 1119 2.3 1 954 165 95 909 2.3 1 766 143 96 735 2.3 1 603 132 97 675 2.3 1 558 117 98 582 2.3 1 454 128 99 551 2.3 1 426 125 100 543 2.3 1 410 133 101 446 2.3 1 338 108 102 419 2.3 1 319 100 103 337 2.3 1 245 92 104 360 2.3 1 263 97 105 290 2.3 1 199 91 106 305 2.3 1 222 83 107 280 2.3 1 187 93 108 268 2.3 1 186 82 109 224 2.3 1 153 71 110 265 2.3 1 179 86 111 214 2.3 1 156 58 112 234 2.3 1 145 89 113 231 2.3 1 160 71 114 198 2.3 1 134 64 115 173 2.3 1 132 41 116 180 2.3 1 124 56 117 162 2.3 1 108 54 118 185 2.3 1 142 43 119 192 2.3 1 134 58 120 185 2.3 1 121 64 121 149 2.3 1 94 55 122 170 2.3 1 115 55 123 140 2.3 1 92 48 124 139 2.3 1 97 42 125 127 2.3 1 90 37 126 127 2.3 1 87 40 127 118 2.3 1 80 38 128 110 2.3 1 73 37 129 93 2.3 1 75 18 130 98 2.3 1 70 28 131 89 2.3 1 62 27 132 80 2.3 1 66 14 133 79 2.3 1 61 18 134 78 2.3 1 53 25 135 65 2.3 1 47 18 136 56 2.3 1 45 11 137 28 2.3 1 24 4 138 30 2.3 1 23 7 139 21 2.3 1 15 6 140 24 2.3 1 15 9 141 23 2.3 1 13 10 142 20 2.3 1 10 10 143 21 2.3 1 8 13 144 25 2.3 1 11 14 145 29 2.3 1 12 17 146 15 2.3 1 8 7 147 17 2.3 1 8 9 148 28 2.3 1 11 17 149 60 2.3 1 11 49 150 671 2.3 1 6 665 RUN STATISTICS FOR INPUT FILE: ERR13913476_1.fastq.gz ============================================= 151439032 sequences processed in total