SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913475_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 10212). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913475_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1957.230 s (18.874 µs/read; 3.18 M reads/minute). === Summary === Total reads processed: 103,702,367 Reads with adapters: 41,169,508 (39.7%) Reads written (passing filters): 103,702,367 (100.0%) Total basepairs processed: 15,555,355,050 bp Quality-trimmed: 22,033,220 bp (0.1%) Total written (filtered): 15,444,347,860 bp (99.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 41169508 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.7% C: 0.2% G: 13.5% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 32293015 25925591.8 0 32293015 2 4881386 6481397.9 0 4881386 3 1873231 1620349.5 0 1873231 4 763412 405087.4 0 763412 5 47025 101271.8 0 47025 6 34143 25318.0 0 34143 7 35346 6329.5 0 35346 8 36744 1582.4 0 36744 9 36909 395.6 0 35972 937 10 38939 98.9 1 32542 6397 11 41213 24.7 1 33473 7740 12 38161 6.2 1 32382 5779 13 34200 1.5 1 28153 6047 14 35166 1.5 1 28048 7118 15 33841 1.5 1 27502 6339 16 35658 1.5 1 28108 7550 17 36620 1.5 1 29356 7264 18 33381 1.5 1 27631 5750 19 28779 1.5 1 23461 5318 20 31216 1.5 1 25477 5739 21 30028 1.5 1 24630 5398 22 28639 1.5 1 23768 4871 23 28819 1.5 1 23565 5254 24 28746 1.5 1 22894 5852 25 27219 1.5 1 22443 4776 26 26418 1.5 1 21867 4551 27 27397 1.5 1 22522 4875 28 24565 1.5 1 20741 3824 29 26546 1.5 1 21584 4962 30 25173 1.5 1 21195 3978 31 21762 1.5 1 18363 3399 32 22164 1.5 1 18631 3533 33 21766 1.5 1 17971 3795 34 21479 1.5 1 17920 3559 35 18949 1.5 1 16125 2824 36 19574 1.5 1 16562 3012 37 18753 1.5 1 15799 2954 38 19372 1.5 1 16325 3047 39 18960 1.5 1 15917 3043 40 16009 1.5 1 13198 2811 41 16667 1.5 1 14136 2531 42 19354 1.5 1 15944 3410 43 11833 1.5 1 10086 1747 44 12944 1.5 1 11227 1717 45 16077 1.5 1 13262 2815 46 13357 1.5 1 10885 2472 47 19538 1.5 1 16892 2646 48 7068 1.5 1 5686 1382 49 10087 1.5 1 8671 1416 50 15063 1.5 1 12964 2099 51 8904 1.5 1 7585 1319 52 11405 1.5 1 9577 1828 53 12453 1.5 1 10415 2038 54 8264 1.5 1 7078 1186 55 5582 1.5 1 4931 651 56 9078 1.5 1 7982 1096 57 10696 1.5 1 9469 1227 58 6181 1.5 1 5306 875 59 9287 1.5 1 8329 958 60 4582 1.5 1 3918 664 61 6391 1.5 1 5510 881 62 3854 1.5 1 3457 397 63 2216 1.5 1 1825 391 64 5715 1.5 1 5039 676 65 5242 1.5 1 4753 489 66 2158 1.5 1 1850 308 67 2606 1.5 1 2233 373 68 5085 1.5 1 4471 614 69 2997 1.5 1 2609 388 70 4298 1.5 1 3755 543 71 3613 1.5 1 3116 497 72 4956 1.5 1 4345 611 73 474 1.5 1 320 154 74 450 1.5 1 336 114 75 1127 1.5 1 964 163 76 1685 1.5 1 1468 217 77 1701 1.5 1 1475 226 78 1762 1.5 1 1462 300 79 1683 1.5 1 1462 221 80 1544 1.5 1 1298 246 81 1476 1.5 1 1242 234 82 1328 1.5 1 1117 211 83 1190 1.5 1 1011 179 84 1112 1.5 1 900 212 85 1051 1.5 1 842 209 86 910 1.5 1 724 186 87 847 1.5 1 675 172 88 807 1.5 1 639 168 89 692 1.5 1 554 138 90 685 1.5 1 526 159 91 619 1.5 1 443 176 92 579 1.5 1 422 157 93 501 1.5 1 368 133 94 493 1.5 1 352 141 95 428 1.5 1 319 109 96 416 1.5 1 292 124 97 322 1.5 1 230 92 98 320 1.5 1 203 117 99 334 1.5 1 224 110 100 309 1.5 1 187 122 101 274 1.5 1 184 90 102 266 1.5 1 179 87 103 255 1.5 1 170 85 104 255 1.5 1 149 106 105 219 1.5 1 121 98 106 213 1.5 1 123 90 107 193 1.5 1 116 77 108 222 1.5 1 127 95 109 199 1.5 1 138 61 110 201 1.5 1 114 87 111 179 1.5 1 114 65 112 193 1.5 1 112 81 113 159 1.5 1 91 68 114 184 1.5 1 109 75 115 162 1.5 1 97 65 116 130 1.5 1 91 39 117 138 1.5 1 73 65 118 132 1.5 1 75 57 119 152 1.5 1 93 59 120 142 1.5 1 87 55 121 119 1.5 1 76 43 122 114 1.5 1 70 44 123 106 1.5 1 65 41 124 96 1.5 1 67 29 125 122 1.5 1 72 50 126 111 1.5 1 78 33 127 77 1.5 1 48 29 128 94 1.5 1 62 32 129 69 1.5 1 48 21 130 88 1.5 1 61 27 131 54 1.5 1 36 18 132 48 1.5 1 30 18 133 36 1.5 1 22 14 134 41 1.5 1 31 10 135 30 1.5 1 14 16 136 31 1.5 1 24 7 137 20 1.5 1 8 12 138 20 1.5 1 10 10 139 19 1.5 1 6 13 140 14 1.5 1 6 8 141 11 1.5 1 5 6 142 14 1.5 1 4 10 143 10 1.5 1 4 6 144 10 1.5 1 0 10 145 11 1.5 1 7 4 146 17 1.5 1 2 15 147 20 1.5 1 5 15 148 26 1.5 1 10 16 149 48 1.5 1 4 44 150 1045 1.5 1 2 1043 RUN STATISTICS FOR INPUT FILE: ERR13913475_1.fastq.gz ============================================= 103702367 sequences processed in total