SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913474_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 21647). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913474_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 2177.417 s (21.212 µs/read; 2.83 M reads/minute). === Summary === Total reads processed: 102,651,278 Reads with adapters: 53,102,109 (51.7%) Reads written (passing filters): 102,651,278 (100.0%) Total basepairs processed: 15,397,691,700 bp Quality-trimmed: 57,447,964 bp (0.4%) Total written (filtered): 15,203,964,046 bp (98.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 53102109 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 52.6% C: 17.3% G: 0.3% T: 29.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 49998625 25662819.5 0 49998625 2 123672 6415704.9 0 123672 3 114826 1603926.2 0 114826 4 87158 400981.6 0 87158 5 82747 100245.4 0 82747 6 67690 25061.3 0 67690 7 71574 6265.3 0 71574 8 76049 1566.3 0 76049 9 61861 391.6 0 61832 29 10 69739 97.9 1 65314 4425 11 68889 24.5 1 63477 5412 12 66578 6.1 1 46223 20355 13 64686 1.5 1 44589 20097 14 66922 1.5 1 47011 19911 15 59204 1.5 1 41422 17782 16 62547 1.5 1 43531 19016 17 74769 1.5 1 52390 22379 18 45865 1.5 1 32111 13754 19 76331 1.5 1 54701 21630 20 51295 1.5 1 35644 15651 21 47276 1.5 1 33354 13922 22 54726 1.5 1 38839 15887 23 53078 1.5 1 37837 15241 24 64006 1.5 1 46056 17950 25 57872 1.5 1 41138 16734 26 41465 1.5 1 29741 11724 27 47095 1.5 1 33648 13447 28 49080 1.5 1 35731 13349 29 49071 1.5 1 35455 13616 30 49465 1.5 1 35956 13509 31 45594 1.5 1 33053 12541 32 45281 1.5 1 33298 11983 33 44178 1.5 1 32303 11875 34 42362 1.5 1 31254 11108 35 41573 1.5 1 30263 11310 36 39767 1.5 1 29512 10255 37 42415 1.5 1 31287 11128 38 36596 1.5 1 27019 9577 39 37740 1.5 1 27811 9929 40 38417 1.5 1 28960 9457 41 34942 1.5 1 25540 9402 42 33118 1.5 1 24807 8311 43 31139 1.5 1 23347 7792 44 33382 1.5 1 25298 8084 45 29389 1.5 1 22078 7311 46 29862 1.5 1 22805 7057 47 29723 1.5 1 22414 7309 48 29687 1.5 1 22500 7187 49 26057 1.5 1 19867 6190 50 26128 1.5 1 19854 6274 51 26821 1.5 1 20548 6273 52 23939 1.5 1 18481 5458 53 23491 1.5 1 18024 5467 54 23093 1.5 1 17707 5386 55 21211 1.5 1 16395 4816 56 22841 1.5 1 17850 4991 57 18018 1.5 1 13895 4123 58 19422 1.5 1 15200 4222 59 22465 1.5 1 17600 4865 60 15997 1.5 1 12380 3617 61 17094 1.5 1 13397 3697 62 17570 1.5 1 13792 3778 63 15049 1.5 1 11801 3248 64 14317 1.5 1 11313 3004 65 24687 1.5 1 19977 4710 66 10359 1.5 1 7935 2424 67 9675 1.5 1 7597 2078 68 9775 1.5 1 7743 2032 69 12804 1.5 1 10246 2558 70 12541 1.5 1 9852 2689 71 9514 1.5 1 7630 1884 72 10225 1.5 1 8262 1963 73 9696 1.5 1 7747 1949 74 9011 1.5 1 7191 1820 75 13093 1.5 1 10523 2570 76 7697 1.5 1 6112 1585 77 5123 1.5 1 4035 1088 78 2346 1.5 1 1884 462 79 3724 1.5 1 3012 712 80 4408 1.5 1 3634 774 81 4297 1.5 1 3471 826 82 3870 1.5 1 3164 706 83 3625 1.5 1 2925 700 84 3352 1.5 1 2691 661 85 3099 1.5 1 2514 585 86 2712 1.5 1 2201 511 87 2401 1.5 1 1905 496 88 2102 1.5 1 1657 445 89 1875 1.5 1 1452 423 90 1681 1.5 1 1293 388 91 1473 1.5 1 1109 364 92 1314 1.5 1 1020 294 93 1097 1.5 1 832 265 94 1001 1.5 1 754 247 95 902 1.5 1 650 252 96 753 1.5 1 526 227 97 695 1.5 1 493 202 98 636 1.5 1 438 198 99 540 1.5 1 375 165 100 478 1.5 1 314 164 101 452 1.5 1 287 165 102 449 1.5 1 267 182 103 342 1.5 1 217 125 104 337 1.5 1 197 140 105 276 1.5 1 156 120 106 300 1.5 1 163 137 107 279 1.5 1 148 131 108 261 1.5 1 129 132 109 267 1.5 1 129 138 110 245 1.5 1 115 130 111 218 1.5 1 103 115 112 203 1.5 1 118 85 113 208 1.5 1 115 93 114 199 1.5 1 113 86 115 167 1.5 1 89 78 116 170 1.5 1 99 71 117 165 1.5 1 82 83 118 180 1.5 1 94 86 119 163 1.5 1 82 81 120 164 1.5 1 84 80 121 157 1.5 1 79 78 122 134 1.5 1 72 62 123 149 1.5 1 81 68 124 129 1.5 1 75 54 125 132 1.5 1 76 56 126 141 1.5 1 77 64 127 102 1.5 1 58 44 128 122 1.5 1 81 41 129 76 1.5 1 44 32 130 76 1.5 1 50 26 131 54 1.5 1 30 24 132 65 1.5 1 47 18 133 34 1.5 1 19 15 134 37 1.5 1 23 14 135 31 1.5 1 23 8 136 29 1.5 1 21 8 137 25 1.5 1 13 12 138 19 1.5 1 17 2 139 16 1.5 1 5 11 140 21 1.5 1 16 5 141 9 1.5 1 6 3 142 5 1.5 1 3 2 143 9 1.5 1 5 4 144 16 1.5 1 10 6 145 11 1.5 1 9 2 146 10 1.5 1 8 2 147 9 1.5 1 4 5 148 9 1.5 1 6 3 149 8 1.5 1 3 5 150 9 1.5 1 3 6 RUN STATISTICS FOR INPUT FILE: ERR13913474_2.fastq.gz ============================================= 102651278 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 102651278 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 20820 (0.02%)