SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913474_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 21647). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913474_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2231.168 s (21.735 µs/read; 2.76 M reads/minute). === Summary === Total reads processed: 102,651,278 Reads with adapters: 41,774,785 (40.7%) Reads written (passing filters): 102,651,278 (100.0%) Total basepairs processed: 15,397,691,700 bp Quality-trimmed: 22,016,662 bp (0.1%) Total written (filtered): 15,242,692,074 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 41774785 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.4% C: 0.2% G: 13.9% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 31668798 25662819.5 0 31668798 2 4772969 6415704.9 0 4772969 3 1856811 1603926.2 0 1856811 4 778442 400981.6 0 778442 5 80605 100245.4 0 80605 6 58640 25061.3 0 58640 7 62101 6265.3 0 62101 8 65012 1566.3 0 65012 9 65511 391.6 0 64606 905 10 64530 97.9 1 57755 6775 11 71349 24.5 1 61143 10206 12 66073 6.1 1 58606 7467 13 60240 1.5 1 52430 7810 14 63650 1.5 1 54067 9583 15 60276 1.5 1 51670 8606 16 62476 1.5 1 52376 10100 17 66175 1.5 1 56164 10011 18 59874 1.5 1 52717 7157 19 51421 1.5 1 44605 6816 20 57500 1.5 1 49604 7896 21 55121 1.5 1 47810 7311 22 52609 1.5 1 46304 6305 23 53554 1.5 1 46558 6996 24 54455 1.5 1 46338 8117 25 51978 1.5 1 45405 6573 26 49340 1.5 1 43406 5934 27 51187 1.5 1 44705 6482 28 46700 1.5 1 41490 5210 29 50730 1.5 1 43609 7121 30 49494 1.5 1 44019 5475 31 41693 1.5 1 37045 4648 32 43945 1.5 1 39005 4940 33 43907 1.5 1 38377 5530 34 43951 1.5 1 38716 5235 35 40898 1.5 1 36066 4832 36 37460 1.5 1 33950 3510 37 40341 1.5 1 35659 4682 38 35414 1.5 1 31903 3511 39 38202 1.5 1 33910 4292 40 35750 1.5 1 32372 3378 41 41774 1.5 1 37203 4571 42 30008 1.5 1 26983 3025 43 28840 1.5 1 25862 2978 44 30983 1.5 1 28184 2799 45 33842 1.5 1 30050 3792 46 30758 1.5 1 26947 3811 47 41943 1.5 1 38537 3406 48 15317 1.5 1 13432 1885 49 22834 1.5 1 20995 1839 50 27511 1.5 1 24989 2522 51 23933 1.5 1 21853 2080 52 24622 1.5 1 22108 2514 53 26343 1.5 1 23286 3057 54 22598 1.5 1 20607 1991 55 18832 1.5 1 17453 1379 56 20211 1.5 1 18509 1702 57 22350 1.5 1 20509 1841 58 17771 1.5 1 16276 1495 59 21735 1.5 1 19990 1745 60 19216 1.5 1 17625 1591 61 16055 1.5 1 14514 1541 62 15300 1.5 1 14294 1006 63 11376 1.5 1 10408 968 64 16284 1.5 1 14997 1287 65 16067 1.5 1 15122 945 66 11667 1.5 1 10809 858 67 9222 1.5 1 8342 880 68 13857 1.5 1 12906 951 69 12666 1.5 1 11737 929 70 15995 1.5 1 14910 1085 71 11891 1.5 1 10875 1016 72 17035 1.5 1 15740 1295 73 1279 1.5 1 1065 214 74 1460 1.5 1 1312 148 75 3956 1.5 1 3706 250 76 5200 1.5 1 4891 309 77 5801 1.5 1 5421 380 78 5726 1.5 1 5301 425 79 5362 1.5 1 4996 366 80 5001 1.5 1 4674 327 81 4672 1.5 1 4307 365 82 4094 1.5 1 3754 340 83 3700 1.5 1 3413 287 84 3366 1.5 1 3086 280 85 3058 1.5 1 2810 248 86 2644 1.5 1 2451 193 87 2261 1.5 1 2058 203 88 2027 1.5 1 1837 190 89 1809 1.5 1 1646 163 90 1571 1.5 1 1409 162 91 1413 1.5 1 1269 144 92 1210 1.5 1 1091 119 93 1015 1.5 1 906 109 94 909 1.5 1 790 119 95 824 1.5 1 703 121 96 682 1.5 1 566 116 97 595 1.5 1 498 97 98 519 1.5 1 433 86 99 424 1.5 1 352 72 100 399 1.5 1 310 89 101 342 1.5 1 277 65 102 332 1.5 1 253 79 103 245 1.5 1 196 49 104 239 1.5 1 170 69 105 207 1.5 1 144 63 106 215 1.5 1 149 66 107 192 1.5 1 121 71 108 199 1.5 1 130 69 109 167 1.5 1 118 49 110 167 1.5 1 122 45 111 139 1.5 1 97 42 112 152 1.5 1 112 40 113 131 1.5 1 101 30 114 121 1.5 1 72 49 115 105 1.5 1 75 30 116 121 1.5 1 83 38 117 105 1.5 1 64 41 118 132 1.5 1 92 40 119 108 1.5 1 70 38 120 132 1.5 1 83 49 121 115 1.5 1 77 38 122 75 1.5 1 51 24 123 100 1.5 1 70 30 124 102 1.5 1 68 34 125 105 1.5 1 72 33 126 97 1.5 1 79 18 127 76 1.5 1 49 27 128 85 1.5 1 60 25 129 65 1.5 1 45 20 130 72 1.5 1 47 25 131 42 1.5 1 34 8 132 59 1.5 1 53 6 133 30 1.5 1 16 14 134 35 1.5 1 27 8 135 27 1.5 1 23 4 136 32 1.5 1 22 10 137 25 1.5 1 23 2 138 24 1.5 1 11 13 139 11 1.5 1 7 4 140 26 1.5 1 20 6 141 18 1.5 1 9 9 142 9 1.5 1 3 6 143 12 1.5 1 6 6 144 23 1.5 1 13 10 145 18 1.5 1 9 9 146 19 1.5 1 9 10 147 16 1.5 1 7 9 148 28 1.5 1 6 22 149 63 1.5 1 3 60 150 830 1.5 1 5 825 RUN STATISTICS FOR INPUT FILE: ERR13913474_1.fastq.gz ============================================= 102651278 sequences processed in total