Bismark report for: ERR13913473_val_1.fq.gz and ERR13913473_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 115344154 Number of paired-end alignments with a unique best hit: 53915399 Mapping efficiency: 46.7% Sequence pairs with no alignments under any condition: 53092319 Sequence pairs did not map uniquely: 8336436 Sequence pairs which were discarded because genomic sequence could not be extracted: 28 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 26961057 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 26954314 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2660193034 Total methylated C's in CpG context: 37054493 Total methylated C's in CHG context: 1142230 Total methylated C's in CHH context: 3951374 Total methylated C's in Unknown context: 85192 Total unmethylated C's in CpG context: 276758949 Total unmethylated C's in CHG context: 438774157 Total unmethylated C's in CHH context: 1902511831 Total unmethylated C's in Unknown context: 7754618 C methylated in CpG context: 11.8% C methylated in CHG context: 0.3% C methylated in CHH context: 0.2% C methylated in Unknown context (CN or CHN): 1.1% Bismark completed in 0d 5h 55m 1s