SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913473_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 8002). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913473_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2555.140 s (22.147 µs/read; 2.71 M reads/minute). === Summary === Total reads processed: 115,373,304 Reads with adapters: 45,578,334 (39.5%) Reads written (passing filters): 115,373,304 (100.0%) Total basepairs processed: 17,305,995,600 bp Quality-trimmed: 23,135,452 bp (0.1%) Total written (filtered): 17,195,655,495 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 45578334 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.1% G: 13.4% T: 48.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 35998569 28843326.0 0 35998569 2 5470363 7210831.5 0 5470363 3 2085722 1802707.9 0 2085722 4 847240 450677.0 0 847240 5 46784 112669.2 0 46784 6 33640 28167.3 0 33640 7 34234 7041.8 0 34234 8 36191 1760.5 0 36191 9 35967 440.1 0 34990 977 10 36844 110.0 1 32241 4603 11 39237 27.5 1 33133 6104 12 35961 6.9 1 31706 4255 13 32506 1.7 1 28231 4275 14 33673 1.7 1 28750 4923 15 32390 1.7 1 27826 4564 16 33091 1.7 1 27570 5521 17 34034 1.7 1 28869 5165 18 30958 1.7 1 27314 3644 19 26334 1.7 1 22983 3351 20 29425 1.7 1 25403 4022 21 27876 1.7 1 24088 3788 22 26360 1.7 1 23147 3213 23 27109 1.7 1 23489 3620 24 26144 1.7 1 22090 4054 25 24751 1.7 1 21584 3167 26 23412 1.7 1 20499 2913 27 23853 1.7 1 20739 3114 28 21714 1.7 1 19281 2433 29 23010 1.7 1 19747 3263 30 21898 1.7 1 19412 2486 31 17956 1.7 1 15929 2027 32 18745 1.7 1 16575 2170 33 18110 1.7 1 15770 2340 34 17282 1.7 1 15350 1932 35 17479 1.7 1 15447 2032 36 17162 1.7 1 14708 2454 37 16769 1.7 1 14844 1925 38 13721 1.7 1 12210 1511 39 15605 1.7 1 13725 1880 40 17227 1.7 1 15604 1623 41 8272 1.7 1 7324 948 42 11633 1.7 1 10440 1193 43 11295 1.7 1 9822 1473 44 10036 1.7 1 9130 906 45 11682 1.7 1 10189 1493 46 10273 1.7 1 8874 1399 47 12562 1.7 1 11516 1046 48 5477 1.7 1 4698 779 49 7220 1.7 1 6569 651 50 7971 1.7 1 7188 783 51 7056 1.7 1 6327 729 52 6983 1.7 1 6125 858 53 7241 1.7 1 6238 1003 54 6408 1.7 1 5635 773 55 4987 1.7 1 4513 474 56 4899 1.7 1 4400 499 57 5517 1.7 1 4965 552 58 4599 1.7 1 4049 550 59 4979 1.7 1 4470 509 60 4744 1.7 1 4179 565 61 3584 1.7 1 3096 488 62 3207 1.7 1 2972 235 63 2740 1.7 1 2386 354 64 3325 1.7 1 2934 391 65 3016 1.7 1 2782 234 66 2609 1.7 1 2286 323 67 1973 1.7 1 1668 305 68 2576 1.7 1 2307 269 69 2567 1.7 1 2266 301 70 3073 1.7 1 2688 385 71 2176 1.7 1 1837 339 72 2957 1.7 1 2568 389 73 354 1.7 1 242 112 74 347 1.7 1 243 104 75 703 1.7 1 596 107 76 988 1.7 1 856 132 77 1036 1.7 1 885 151 78 998 1.7 1 855 143 79 949 1.7 1 793 156 80 953 1.7 1 791 162 81 812 1.7 1 679 133 82 843 1.7 1 694 149 83 734 1.7 1 592 142 84 640 1.7 1 506 134 85 631 1.7 1 472 159 86 606 1.7 1 487 119 87 546 1.7 1 436 110 88 519 1.7 1 379 140 89 494 1.7 1 372 122 90 483 1.7 1 358 125 91 417 1.7 1 296 121 92 414 1.7 1 309 105 93 372 1.7 1 279 93 94 371 1.7 1 272 99 95 374 1.7 1 243 131 96 330 1.7 1 222 108 97 304 1.7 1 199 105 98 272 1.7 1 177 95 99 265 1.7 1 177 88 100 280 1.7 1 185 95 101 256 1.7 1 157 99 102 254 1.7 1 154 100 103 226 1.7 1 153 73 104 218 1.7 1 146 72 105 207 1.7 1 132 75 106 173 1.7 1 116 57 107 189 1.7 1 125 64 108 205 1.7 1 118 87 109 175 1.7 1 110 65 110 181 1.7 1 120 61 111 179 1.7 1 113 66 112 162 1.7 1 101 61 113 156 1.7 1 99 57 114 157 1.7 1 88 69 115 114 1.7 1 74 40 116 135 1.7 1 92 43 117 116 1.7 1 75 41 118 129 1.7 1 76 53 119 108 1.7 1 69 39 120 114 1.7 1 80 34 121 99 1.7 1 57 42 122 98 1.7 1 69 29 123 107 1.7 1 60 47 124 100 1.7 1 68 32 125 89 1.7 1 58 31 126 98 1.7 1 66 32 127 70 1.7 1 42 28 128 101 1.7 1 63 38 129 66 1.7 1 44 22 130 52 1.7 1 31 21 131 44 1.7 1 29 15 132 49 1.7 1 31 18 133 33 1.7 1 20 13 134 47 1.7 1 32 15 135 27 1.7 1 20 7 136 30 1.7 1 19 11 137 18 1.7 1 12 6 138 19 1.7 1 14 5 139 19 1.7 1 10 9 140 13 1.7 1 7 6 141 11 1.7 1 6 5 142 7 1.7 1 2 5 143 11 1.7 1 5 6 144 8 1.7 1 2 6 145 8 1.7 1 2 6 146 8 1.7 1 2 6 147 17 1.7 1 4 13 148 19 1.7 1 5 14 149 31 1.7 1 1 30 150 563 1.7 1 1 562 RUN STATISTICS FOR INPUT FILE: ERR13913473_1.fastq.gz ============================================= 115373304 sequences processed in total